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Citations for New England Biolabs :
301 - 350 of 1372 citations for IGFBP2 Human T7 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... These genes were transformed into T7 SHuffle (New England Biolabs) competent E ...
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bioRxiv - Biochemistry 2023Quote: All the variants were transformed into SHuffle T7 cells (NEB) and plated on LB plates with kanamycin (kana ...
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bioRxiv - Developmental Biology 2023Quote: ... sgRNAs were synthesized using the HiScribe T7 kit (NEB, E2050S) and purified by the RNA purification Kit (Zymogen ...
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bioRxiv - Immunology 2024Quote: ... The HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) was used to transcribe the template DNA per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5 μL of T7 Endonuclease I (New England Biolabs®) was then mixed into one tube of each sample ...
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bioRxiv - Microbiology 2019Quote: ... or Bam HI and Xho I (New England BioLabs, MA). The column isolated DNA fragments were ligated into our modified pFastBac1 plasmid having 6xHis tag at C-terminus ...
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bioRxiv - Molecular Biology 2020Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 (Illumina).
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bioRxiv - Microbiology 2021Quote: ... Followed by subcloning the PCR amplicon in Bam-HI (NEB) restriction digested pCW57-tGFP-2A-MCS plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... using NEBuilder Hi-Fi Assembly (New England Biolabs, Cat. #E2621). The pMB1052 construct was subsequently cut using BspDI and EcoRV (New England Biolabs) ...
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bioRxiv - Genomics 2022Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB).
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bioRxiv - Systems Biology 2024Quote: ... using the NEBuilder Hi-Fi Assembly kit (New England Biolabs). To ensure representation of all variants in the population ...
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bioRxiv - Cancer Biology 2023Quote: ... cut with hi-fidelity EcoRI and BamHI (New England Biolabs), and gel purified ...
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bioRxiv - Immunology 2021Quote: ... and in vitro transcribed by T7 RNA polymerase (Cat: M0251, NEB) in the presence of a trinucleotide cap1 analogue ...
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bioRxiv - Developmental Biology 2021Quote: ... and each amplicon was digested with T7 Endonuclease I (NEB; M0302S). Digested amplicons were run on agarose gels to quantify relative gRNA activity ...
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bioRxiv - Bioengineering 2019Quote: ... Mutated plasmids were transformed into T7 Express cells (New England BioLabs), plated on LB+100 µg/mL ampicillin agar plates and grown overnight at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... and digestion of the amplicon using T7 endonuclease (New England Biolabs). Outcrossed F1 fish that contained a 25bp deletion were isolated and raised to adulthood.
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bioRxiv - Cell Biology 2019Quote: ... Genome indel was assessed by T7 Endonuclease I assay (NEB #M0302) followed by SDS-PAGE gel electrophoresis (Genscript #M42012L) ...
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bioRxiv - Synthetic Biology 2021Quote: ... A HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB) was used for crRNA in vitro transcription ...
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bioRxiv - Systems Biology 2021Quote: ... 0.5 μl T7 DNA ligase (NEB New England Biolabs, Ipswich, MA) and 0.5 μl BsaI (Eco31I ...
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bioRxiv - Synthetic Biology 2021Quote: ... 12 mM MgCl2 and 1 U/ μl T7 Polymerase (all NEB). The transcription mix was incubated at 37°C for 4 h ...
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bioRxiv - Systems Biology 2021Quote: ... 0.5 μl T7 DNA ligase (NEB New England Biolabs, Ipswich, MA) and 0.5 μl BsaI (Eco31I ...
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bioRxiv - Cell Biology 2021Quote: ... SgRNA are produced by T7 Hiscribe transcription kit (New England Biolabs) and purified by EZNA microelute RNA clean up kit (Omega biotek) ...
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bioRxiv - Biophysics 2020Quote: MBP and dmMBP were overexpressed in T7 competent cells (NEB laboratories) in LB medium supplemented with 0.2% glucose and 50 μg/ml kanamycin at 30°C until OD600~0.6 ...
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bioRxiv - Systems Biology 2022Quote: ... and products were in vitro transcribed overnight using T7 polymerase (NEB) for linear amplification ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1 µl of T4 or T7 DNA Ligase (New England Biolabs), 1 µl of BsaI-HFV2 (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2022Quote: ... except for pRO16Sh5 which was blunt ligated using T7 ligase (NEB).The assemblies were transformed into chemically competent E ...
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bioRxiv - Immunology 2022Quote: A HiscribeTM T7 ARCA mRNA Kit (with tailing) (NEB, Cat # E2060S) was used to in vitro transcribe codon-optimized mRNA encoding HexaPro spikes of SARS-CoV-2 WT and Omicron variant with 50% replacement of uridine by N1-methyl-pseudouridine ...
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bioRxiv - Microbiology 2022Quote: ... and then immediately subjected to restriction digestion with T7 endonuclease (NEB) which cleaves at DNA cruciforms ...
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bioRxiv - Immunology 2022Quote: ... and in vitro transcribed by T7 RNA polymerase (Cat: M0251, NEB) in the presence of a trinucleotide cap1 analogue ...
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bioRxiv - Microbiology 2019Quote: ... 5 mM DTT (Thermo) and 250 U T7 RNA polymerase (NEB). The next day ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 T7 Express lysY/Iq cells (New England BioLabs, C3013). The cells were grown in LB medium containing 50 μg/ml kanamycin at 37°C until OD600 nm 0.5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Add 1 μl T7 DNA ligase (New England Biolabs, MA, USA) (Important ...
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bioRxiv - Microbiology 2020Quote: ... RNAs were synthesized with phage T7 RNA Polymerase (25 units, NEB) in 50µl reaction containing 1X T7-RNA Polymerase buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR product was transcribed using the T7 IVT Kit (NEB), followed by DNase I (NEB ...
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bioRxiv - Genetics 2020Quote: ... using HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB) and purified using RNeasy Mini Kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... RNA was transcribed using HiScribe T7 RNA polymerase (New England Biolabs) and purified by denaturing polyacrylamide gel electrophoresis (PAGE ...
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bioRxiv - Physiology 2021Quote: ... T7 RNA polymerase mediated in vitro transcription (NEB, Frankfurt, Germany, E2040S) was used for sgRNA-generation from the pSpCas9(BB)-2A-GFP (PX458 ...
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bioRxiv - Biochemistry 2021Quote: ... RNA was transcribed using HiScribe T7 RNA polymerase (New England Biolabs) and purified by denaturing polyacrylamide gel electrophoresis (PAGE) ...
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bioRxiv - Immunology 2020Quote: HiScribe™ T7 ARCA mRNA Kit (with tailing) (New England Biolabs);
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bioRxiv - Genomics 2021Quote: ... The HiScribe T7 High-Yield RNA Synthesis Kit (New England BioLabs) was used according to the company’s recommended instruction but with full substitution of N1-methyl-pseudouridine for uridine and co-transcriptional capping with CleanCap AG (TriLink Biotechnologies) ...
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bioRxiv - Molecular Biology 2022Quote: ... After mixing the annealed DNA template with T7 RNA polymerase (NEB) and the reaction mixture [50 mM MgCl2 ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was transcribed using the manufacturers protocol for HiScribe T7 (NEB). RNA was treated with DNase I (NEB) ...
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bioRxiv - Genomics 2022Quote: ... The three arms were ligated by T7 ligase (NEB, Cat# M0318L), at 25 C overnight ...
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bioRxiv - Genomics 2022Quote: The HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) was used for the in vitro synthesis of RNA transcripts from a FLuc control template ...
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bioRxiv - Molecular Biology 2022Quote: ... using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040). Our standard 10 μL reactions used 0.5 μg of linear plasmid template and an 8:1 ARCA:GTP ratio.
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bioRxiv - Molecular Biology 2022Quote: ... transcribed in vitro with the T7 RNA polymerase (New England Biolabs), and in vitro translated with a Rabbit Reticulocyte Lysate system (Promega).
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bioRxiv - Biophysics 2023Quote: ... Mismatched duplexes were then cleaved by T7 Endonuclease I (NEB M0302S) and cleavage products were detected by gel electrophoresis ...
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bioRxiv - Immunology 2023Quote: ... adding a T7 promoter and an AG initiation sequence (Phusion, NEB): Fw primer ...
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bioRxiv - Genomics 2023Quote: ... Template were first digested with T7 endonuclease I (M0302L, NEB, USA) as suggested in the protocol available on the ONT community website ...
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bioRxiv - Molecular Biology 2023Quote: ... using the HiScribe T7 High Yield RNA Synthesis Kit (NEB E2040S). Transcribed gRNA was cleaned using the RNA Clean and Concentrator kit (Zymo R1013).