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Citations for New England Biolabs :
351 - 400 of 4746 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Each PCR2 reaction contains 25µL of Q5 High-Fidelity 2X Master Mix (NEB), 2.5µL of a unique Nuc PCR#2 Fwd Primer (10µM) ...
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bioRxiv - Cancer Biology 2022Quote: ... Each “PCR1” reaction contains 25µL of Q5 High-Fidelity 2X Master Mix (NEB), 2.5µL of Nuc PCR#1 Fwd Primer (10µM) ...
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bioRxiv - Developmental Biology 2022Quote: ... using the NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs #M0451S) with Nextera DNA CD Indexes (Illumina #20015882) ...
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bioRxiv - Genetics 2022Quote: Conversion tracts were PCR amplified using OneTaq 2x Master Mix (New England Biolabs). Noncrossover recombination products were amplified using forward primer DLO822 (5’-ATTTTAACCCTTCGGGGTACG-3’ ...
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bioRxiv - Biophysics 2022Quote: ... We performed routine cloning using Q5 high-fidelity 2X master mix (NEB M0492) for PCR amplification and isothermal assembly using 2X Gibson master mix (NEB E2611 ...
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bioRxiv - Biophysics 2022Quote: ... for PCR amplification and isothermal assembly using 2X Gibson master mix (NEB E2611) before transformation into NEB Stable or 5-alpha chemically competent cells.
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bioRxiv - Developmental Biology 2021Quote: ... and then amplified with NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L). Libraries were sequenced on HiSeq 125bp paired-end platform.
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bioRxiv - Genetics 2019Quote: ... and 10 µL 2X Hot Start Taq PCR Master Mix (New England Biolabs). The cycling conditions in the Bio-Rad T100™ Thermal Cycler were ...
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bioRxiv - Genetics 2019Quote: ... and 12.5 µL 2X Hot Start Taq PCR Master Mix (New England Biolabs). Samples were run on a Bio-Rad T100™ Thermal Cycler and cycled for 5 minutes at 95°C ...
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bioRxiv - Genetics 2019Quote: ... and 10 µL 2X Hot Start Taq PCR Master Mix (New England Biolabs). Samples were loaded into a Biorad T100™ Thermal Cycler for 3 minutes at 95°C ...
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bioRxiv - Microbiology 2019Quote: ... 20] and LongAmp™ Taq 2X Master Mix (New England Biolabs, Ipswich, MA). Amplification was performed using an Applied Biosystems Veriti™ Thermal Cycler (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2019Quote: ... PCR reactions were performed using Q5® High-Fidelity 2x Master Mix (NEB) for cloning and sequencing otherwise DreamTaq Green PCR Master Mix 2x (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2019Quote: ... We used the Q5 High-Fidelity Polymerase 2x Master Mix (New England Biolabs) to amplify genes and vectors under the following general conditions ...
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bioRxiv - Immunology 2020Quote: ... using Q5® Hotstart High-Fidelity 2X Master Mix (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Immunology 2020Quote: ... using Q5® Hotstart High-Fidelity 2X Master Mix (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Synthetic Biology 2019Quote: ... libraries were amplified using Q5 High-Fidelity 2X Master Mix (New England Biolabs), a final primer concentration of 0.8 µM ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were then amplified with 2X NEBNext master mix (NEB, Ipswitch, MA M0541S) using cycle numbers determined by qPCR as described in the standard ATAC-seq protocol (Buenrostro et al. ...
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bioRxiv - Genomics 2021Quote: ... 25 μL NEBNext Hi-Fi 2x PCR Master Mix (NEB (Ipswich, MA, USA) M0541S) ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCRs were performed using Q5® High-Fidelity 2X Master Mix (NEB #M0492L), sfGfp as the template ...
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bioRxiv - Genetics 2021Quote: ... PCR amplicons were generated using Q5® High-Fidelity 2X Master Mix (NEB) using primers PKS12_F2 and PKS12_R2 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then amplified twice using 2x Q5 Master Mix (New England BioLabs) and two unique primer sets ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were performed with Taq 2X Master Mix (New England BioLabs, M0270L). The thermocycler conditions for Mouse Chk1 and GAPDH PCR product were as follows ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA (20 ng) was PCR amplified using LongAmpTaq 2X master mix (NEB). The PCR amplicons of selected regions were subjected to Sanger sequencing and BLAST analysis to confirm the presence of eight variants using the primers listed in Supplemental Table 1.
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bioRxiv - Genetics 2020Quote: ... 50 μl LongAmp Taq 2x master mix (New England Biolabs, Ipswich, MA, USA). Initial denaturation was for 3 min at 95 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Q5-high Fidelity 2X master mix was used as polymerase from NEB (# M0429L). PCR condition for the first PCR was ...
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bioRxiv - Developmental Biology 2022Quote: ... Libraries were prepared using NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541) with the following conditions ...
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bioRxiv - Cell Biology 2022Quote: ... The first reaction with Q5 Hot Start High-Fidelity 2X Master Mix (NEB) amplifies the gRNA cassette (primers #1 and #2 for CRISPRi and small pooled screen libraries ...
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bioRxiv - Synthetic Biology 2023Quote: ... from PCR fragments obtained with Q5 High-Fidelity 2X Master Mix (#M0492L, NEB). eolTRPA1 coding sequence from Texas Rat snake (Elaphe (Pantherophis ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1x of OneTaq Hot Start 2X Master Mix with Standard Buffer (NEB, M0484L), and 1x Chai Green Dye (CHAI ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1x of OneTaq Hot Start 2X Master Mix with Standard Buffer (NEB, M0484L). The qPCR was performed on CFX96 Touch Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... Samples were then amplified twice using 2x Q5 Master Mix (New England BioLabs) and two unique primer sets ...
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bioRxiv - Evolutionary Biology 2022Quote: ... using 25 ul of Q5 Hot Start Master Mix (2X) (New England Biolabs) for a total volume reaction of 50 ul.
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bioRxiv - Evolutionary Biology 2022Quote: ... using 25 ul of Q5 Hot Start Master Mix (2X) (New England Biolabs) for a total volume reaction of 50 ul.
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A laboratory framework for ongoing optimisation of amplification based genomic surveillance programsbioRxiv - Genomics 2023Quote: ... Each PCR contained 12.5μL Q5 High Fidelity 2x Master Mix (New England Biolabs), 1.1μL of pooled primers mixes at 10μM (final concentration of each primer was ∼10-11pM) ...
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bioRxiv - Microbiology 2023Quote: ... Linearized pExTra plasmid was prepared via PCR (NEB Q5 HotStart 2X Master Mix) of pExTra01 (Heller et al ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA PCR was performed with using OneTaq® 2X Master Mix (NEB) and used primer pairs were ...
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bioRxiv - Evolutionary Biology 2023Quote: ... All PCR mixtures used Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the specified primers and templates below ...
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bioRxiv - Genomics 2023Quote: ... The PCR was performed using Q5 High-Fidelity 2X Master Mix (NEB, M0492L) with conditions described in Supplementary Table 4.
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bioRxiv - Biophysics 2023Quote: ... and Q5 Hot Start High-Fidelity 2X PCR Master Mix (New England Biolabs). The transcribed region had the following spacings ...
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bioRxiv - Plant Biology 2022Quote: ... using a LongAmp Hot Start Taq 2x Master Mix (M0533S, New England Biolabs), and 16S barcoded primers.
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bioRxiv - Genetics 2023Quote: ... 10 μl of Q5 Hot Start High-Fidelity 2x Master Mix (NEB M0494S), 1 μl of 10 μM Primer 1 (5’-ATGAAGCTACTGTCTTCTATCGAACAAGC-3’ ...
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bioRxiv - Cell Biology 2024Quote: ... and PCR was performed using Q5® High-Fidelity 2X Master Mix (NEB). The splicing efficiency is monitored using RT-PCR with primers located on two adjacent exons ...
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bioRxiv - Microbiology 2023Quote: ... 25 µl NEBNext High-Fidelity 2X PCR Master Mix (NEB, catalog number M0541S). In each 50 µl reaction ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed using the Phusion Hot Start Flex 2X Master Mix (NEB) in a GeneAmp PCR system 9700 (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... and amplified and indexed with NEBNext High- Fidelity 2X PCR Master Mix (NEB) and custom primers ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... coli DH5alpha colonies using OneTaq® 2X Master Mix (New England Biolabs, USA) to confirm the correct insertions and further sequenced with Sanger method (Eurofins Scientific ...
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bioRxiv - Microbiology 2023Quote: PCR was performed using Q5 High-Fidelity 2X Master Mix (New England Biolabs) as per manufacturer’s instructions with the following primers ...
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bioRxiv - Developmental Biology 2024Quote: ... and screened by colony PCR with OneTaq Quick-Load 2X Master Mix (NEB). Positive colonies were cultured overnight (o/n ...
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bioRxiv - Neuroscience 2024Quote: ... and Q5 High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA). RT-PCR protocol was involved in 35 cycles of PCR at 98°C for 10 sec ...
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bioRxiv - Genomics 2024Quote: ... mixed with 50 µL 2x NEBuilder HiFi DNA assembly Master mix (NEB E2621L) and incubated at 50 °C for 2 hr ...