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Citations for New England Biolabs :
251 - 300 of 4746 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Quantitative PCR (qPCR) with the Luna Sybr-Green mastermix (NEB) used primer sequences in Table S3 and 2 μL of a 1:10 dilution of the cDNA ...
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bioRxiv - Microbiology 2023Quote: ... MA) or GoTaq Green Master Mix (NEB, Ipswich, MA). The pFLP2 vector was used for all allelic exchange mutants ...
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bioRxiv - Genetics 2023Quote: ... PCR reactions (15 μL) contained 7.5 μL of 2X OneTaq 2X Master Mix with Standard Buffer (NEB), 5.9 μL H2O ...
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bioRxiv - Microbiology 2020Quote: ... 12.5 μL of 2x Hot Start Taq master mix (New England BioLabs), and 7 μL of water (=25 μL total volume) ...
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bioRxiv - Microbiology 2020Quote: ... 25 μL 2x NEBnext High-Fidelity PCR Master Mix (New England Biolabs) and 10 μL nuclease-free distilled H2O (ThermoFisher Scientific) ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... All assemblies were performed with NEB HiFi 2X Master Mix (NEB #E2621L). Assembled constructs were transformed into XL-10 Gold Ultracompetent E ...
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bioRxiv - Genetics 2021Quote: ... or Q5® High-Fidelity 2X Master Mix (New England Biolabs M0492S) and purified with the Qiagen QIAquick PCR Purification Kit (Qiagen 28104).
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bioRxiv - Genomics 2020Quote: ... or Q5 Hot Start High-Fidelity 2x Master Mix (New England Biolabs). Endo-free plasmids used for mammalian transfection were prepped using ZymoPURE II Plasmid Midiprep (Zymo Research Corporation ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed with Taq 2X Master Mix (New England BioLabs, Maine), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR genotyping was performed using Q5 High-Fidelity 2X Master Mix (NEB) with the primer pairs listed in Table 2 ...
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bioRxiv - Bioengineering 2019Quote: ... Target sites were amplified using High Fidelity 2X PCR Master Mix (NEB). Primers used for PCR are listed in Supplementary Materials ...
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bioRxiv - Genetics 2019Quote: ... The gBlocks were amplified using Q5 high fidelity 2X master mix (NEB) and gBlock amplification primers (Arbab et al. ...
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bioRxiv - Neuroscience 2021Quote: ... PCRs were performed using OneTaq 2X Master Mix with Standard Buffer (NEB) using the following primers ...
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bioRxiv - Microbiology 2020Quote: ... LongAmp™ Taq 2x Master Mix (New England Biolabs, Ipswich, MA, USA), and Chai Green Dye 20x (Chai ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA assembly was performed using NEBuilder 2x Master Mix (New England Biolabs). Standard ligation of restriction enzyme digest products was performed using the Roche Rapid Ligation Kit (Merck Life Sciences) ...
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bioRxiv - Developmental Biology 2021Quote: ... gDNA was then used in Q5 High-Fidelity 2X Master Mix (NEB) PCR reactions according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 50μL Q5® Hot Start High-Fidelity 2X Master Mix (NEB # M0494L), and 5μL of a 10μM solution of each primer (P5 and P7) ...
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bioRxiv - Neuroscience 2020Quote: ... plus 25 μl NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L) was added to 20 μl of purified transposed DNA ...
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bioRxiv - Genetics 2020Quote: ... 25 μL of High-Fidelity 2X PCR Master Mix (New England BioLabs), 2.5 uL of indexing primer and 10 μL of DDW were added to the 10 μL of purified DNA and the DNA was amplified five cycles using the following PCR program ...
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bioRxiv - Synthetic Biology 2019Quote: ... and Q5 Hot Start High-Fidelity 2x Master Mix (New England Biolabs) in a final volume of 25 µL.
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bioRxiv - Microbiology 2021Quote: ... using the Q5 High-Fidelity 2X Master Mix (New England Biolabs, NEB), then digestions with restriction enzymes and ligation were performed ...
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bioRxiv - Microbiology 2021Quote: ... using the Q5 High-Fidelity 2X Master Mix (New England Biolabs, NEB), then digestions with restriction enzymes and ligation were performed ...
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bioRxiv - Evolutionary Biology 2020Quote: - 200μL OneTaq Hot Start 2X Master Mix with Standard Buffer (NEB M0484L)
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were generated using the 2x Q5 PCR Master Mix (NEB) in accordance with manufacturer instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and amplified using NEBNext® High-Fidelity 2X PCR Master Mix (NEB). The number of cycles was estimated by qPCR ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... For all PCR reactions Q5 High-Fidelity 2X Master Mix (NEB M0492L) was used ...
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bioRxiv - Microbiology 2020Quote: ... 12.5 μL of 2x Hot Start Taq master mix (New England BioLabs) and 10.5 μL of water (= 25 μL total volume) ...
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bioRxiv - Microbiology 2020Quote: ... 10 μL of 2x Hot Start Taq master mix (New England BioLabs) and 8.18 μL of water (= 20 μL total) ...
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bioRxiv - Microbiology 2020Quote: ... 25 μL of 2x Hot Start Taq master mix (New England BioLabs) and 21.34 μL of water (=50 μL total volume) ...
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bioRxiv - Microbiology 2020Quote: ... 12.5 μL of 2x Hot Start Taq master mix (New England BioLabs), and 10.5 μL of water (=25 ul total volume) ...
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bioRxiv - Microbiology 2020Quote: ... 5 μL 2x NEBnext High-Fidelity PCR Master Mix (New England Biolabs) and 2.9 μL nuclease-free distilled H2O (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2020Quote: ... or Q5 Hot Start High-Fidelity 2x Master Mix (New England Biolabs). All plasmids used in this work were freshly prepared from 50 mL of Mach1 culture using ZymoPURE Plasmid Midiprep (Zymo Research Corporation ...
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bioRxiv - Immunology 2020Quote: ... and Q5 Hot Start High-Fidelity 2x master mix (NEB, Ipswich, MA) and the following protocol ...
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bioRxiv - Genomics 2021Quote: ... or Q5 Hot Start High-Fidelity 2x Master Mix (New England BioLabs) unless otherwise noted ...
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bioRxiv - Bioengineering 2021Quote: ... Q5 High-fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA) was used with the following cycling conditions ...
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bioRxiv - Bioengineering 2022Quote: ... then PCR amplified using the Q5 High-Fidelity 2X Master Mix (NEB). For the tetrahedra with 5 and 7 helical turns per edge ...
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bioRxiv - Genomics 2022Quote: ... and 25μl Q5 High Fidelity 2X Master Mix (M0492, New England Biolabs) in 50μl total volume with 10 cycles amplification ...
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bioRxiv - Microbiology 2022Quote: ... 12.5 µl Quick-Load Taq 2X Master Mix (M0271L, New England Biolabs) and 2 µl template (colony resuspended in TE-T) ...
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bioRxiv - Genomics 2022Quote: ... and 20 μL NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L) were added to each well ...
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bioRxiv - Bioengineering 2023Quote: ... 50 μl of Q5 High-Fidelity 2x Master Mix (New England Biolabs) and water.
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bioRxiv - Microbiology 2023Quote: ... Plasmids were assembled via Gibson Assembly (New England BioLabs 2X Master mix) (62 ...
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bioRxiv - Microbiology 2023Quote: ... Genes were PCR-amplified (New England Biolabs Q5 HotStart 2X Master Mix) from a high-titer Hammy lysate using a forward primer complementary to the first 15-25 bp of each gene sequence (Integrated DNA Technologies) ...
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bioRxiv - Bioengineering 2023Quote: (Optional) OneTaq Quick-Load 2X Master Mix (NEB cat. No. M0486S/L), for genotyping only
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bioRxiv - Cell Biology 2023Quote: ... Genomic PCRs were performed using Hot Start Taq 2x Master Mix (NEB). PTGER2 primers that bind to conserved sequences in both human and mouse were used as internal control ...
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bioRxiv - Genomics 2023Quote: ... and amplified using NEBnext High-Fidelity 2x PCR Master Mix (NEB, M0541L) and custom indexed primers68 ...
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bioRxiv - Molecular Biology 2024Quote: ... Library was prepared with NEBNext High-Fidelity 2X PCR Master Mix (NEB) and SYBR Green I (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... and amplified using high-fidelity 2X PCR Master Mix (New England Biolabs) using primers with standard ATAC-seq barcodes (Buenrostro et al. ...
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bioRxiv - Immunology 2024Quote: ... in 2X NEBNext® Ultra™ II Q5® Master Mix (NEB) subjected to the following reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 µl of NEBNext HiFi 2x PCR Master Mix (New England BioLabs) was mixed with 21 µl of the CUT&Tag DNA ...
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bioRxiv - Genomics 2024Quote: RT-PCR was conducted using Quick-Load Taq 2X Master Mix (NEB). For molecular cloning applications Q5 High-Fidelity DNA polymerase (NEB ...