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Citations for New England Biolabs :
3451 - 3500 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... was performed to amplify the DNA sequence around the knockin position using Phusion high-fidelity DNA polymerase master mixture reaction (NEB); forward primer (5’-CTT CGG AAT GAG TCT CTG TCA TC-3’ ...
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bioRxiv - Neuroscience 2024Quote: ... Capsid library cDNA was then amplified in multiple 50-µL PCR reactions unsing Q5 HotStart high-fidelity 2X master mix (NEB).
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bioRxiv - Synthetic Biology 2023Quote: All PCR and ligation reactions were performed with OneTaq polymerase and Gibson Assembly Master Mix from New England Biolabs (NEB), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were genotyped by polymerase chain reaction (PCR) using isopropanol-precipitated genomic DNA as template and OneTaq DNA polymerase (New England Biolabs, (NEB), #M0480 ...
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bioRxiv - Microbiology 2023Quote: ... Polymerase chain reactions (PCR) were performed with Taq polymerase (Feldan) for screening purposes or Q5 high-fidelity DNA polymerase (New England Biolabs) for cloning and site-directed mutagenesis ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.25 µM of forward and reverse primers mixed in a final volume of 25 µl reaction using Q5 Hot Start High-Fidelity 2X Master Mix (NEB), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The double stranded DNA templates were then synthesized through a 200 µL PCR reaction containing 10x Taq Buffer (NEB, #B9014S), 250 µM dNTP Mix (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Targets were then transcribed to RNA using the T7 HiScribe High Yield RNA Synthesis Kit in 55 μL reactions (New England Biolabs) with a 16h incubation step at 37 °C and purified with 1.8X volume AMPure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... sgRNA amplicons were generated by PCR of 2 ug genomic DNA per 100 ul reaction with Q5 2X Hot Start Master Mix (M0494L, NEB) and enough reactions to maintain 1000x screening sgRNA representation ...
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bioRxiv - Neuroscience 2023Quote: ... as wildtype or T205A variant were amplified by polymerase chain reaction (PCR) using Q5 High-Fidelity master mix (M0492, New England Biolabs (NEB)) from previous plasmid constructs 16 ...
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bioRxiv - Microbiology 2023Quote: ... 50 fmol of each amplicon and 15 fmol of the YCpBAC vector were assembled using the standard Gibson assembly reaction (NEB), transformed into E ...
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bioRxiv - Bioengineering 2024Quote: ... input genomic DNA was amplified in a 10-μl reaction for 26 cycles using NEBNext High-Fidelity 2x PCR Master Mix (NEB). PCR products were purified using Sera-Mag magnetic beads (Cytiva ...
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bioRxiv - Genomics 2023Quote: ... A transcription reaction was then carried out on the purified and quantified dsDNA using the T7 HiScribe transcription kit (NEB). The T7 RNA Polymerase recognizes the T7 promoter region that seeds transcription of the adjacent 20-mer target sequence thus generating the targeting sgRNA for Cas9-mediated nicking of genomic DNA templates ...
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bioRxiv - Systems Biology 2024Quote: Approximately 6ng of each loxcode cassette was combined with approximately 75ng of the linear digested plasmid pool in a final 10uL of 1x HiFi assembly reaction (NEB). This was incubated on a thermocycler at 50C for 15min ...
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bioRxiv - Systems Biology 2024Quote: ... The samples then underwent two separate digestion reactions (with up to 4 µg of genomic DNA) using NlaIII and MseI enzymes (NEB) at 37°C overnight ...
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bioRxiv - Immunology 2022Quote: ... 1µl of the reverse transcribed cDNA was used in each reaction for qPCR using the Luna Universal qPCR Master Mix (NEB) in a 10µl final reaction in a 384 well plate ...
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bioRxiv - Cell Biology 2022Quote: MKLP1(1-711)-3xFLAG-Avi was cloned by stitching four oligonucleotide primer sequences together into a digested MKLP1(1-711)-Avitag plasmid using a HiFi DNA assembly reaction kit (M5520A, New England Biolabs). Ten 10cm plates were seeded with COS-7 cells and each plate was co-transfected 24 hours later with 4.08 μg MKLP1(1-711)-3xFLAG-Avi and 4.08 μg HA-BirA plasmids using TransIT-LT1 transfection reagent (Mirus) ...
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bioRxiv - Microbiology 2022Quote: ... Strand-specific CHIKV RNA primers were chosen from previous work [54] to amplify nucleotides 1-1560 of the CHIKV genome and used in reverse transcription reactions with the LunaScript8482 RT SuperMix Kit (NEB), followed by qPCR using the 2x qPCRBIO SyGreen Blue Mix (PCRBIOSYSTEMS) ...
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bioRxiv - Molecular Biology 2022Quote: ... on-bead decapping and phosphorylation were performed in a 30 μl reaction with 5 units T4 PNK 3’ phosphatase minus (NEB), 2.25 μg GST-Dcp1-Edc1-Dcp2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Ninety-six 12 μl Gibson reactions were performed in 96-well PCR plates using Gibson Assembly Master Mix (NEB E2611L). The Gibson reaction mix was transformed into Mach1 competent cells (4 μl Gibson into 40 μl cells ...
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bioRxiv - Bioengineering 2022Quote: All gene amplifications were performed using polymerase chain reaction (PCR) in a 50 µL mix with Q5 High-Fidelity DNA Polymerase (New England Biolabs) according to the manufactureŕs protocol ...
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bioRxiv - Bioengineering 2022Quote: ... a gene-specific oligonucleotide was annealed to a constant oligonucleotide encoding the CRISPR-Cas9 scaffold and then a fill-in reaction was performed using T4 DNA polymerase (New England Biolabs). For the tyr_1 and tyr_2 sites ...
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bioRxiv - Biophysics 2022Quote: ... The pACEbac1 XSMC1 XSMC3-Halo-Flag and pIDC XRAD21-8xHis were then combined by a Cre recombinase reaction (New England Biolabs). To generate the baculoviruses ...
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bioRxiv - Biophysics 2022Quote: ... The reaction was buffer exchanged in a Microspin G-50 Column (Cytiva) before treatment with Quick Calf Intestinal Phosphatase (CIP, NEB). The plasmid was digested with AgeI ...
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bioRxiv - Bioengineering 2022Quote: ... 1 μL supernatant in a total reaction volume of 20 μL was used as template and PCR was performed using Q5 polymerase (NEB). Primers are listed in Table S1 ...
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bioRxiv - Cell Biology 2022Quote: ... It was followed by a second In-Fusion HD cloning of a polymerase chain reaction using 5’-GAAGGGGATCCACCGATGGTGAGCAAGGGCGAGG-3’ / 5’-TTAGTAGCTCTAGACTTGTACAGCTCGTCCATGCC-3’ (mScarlet insert) using BamHI and XbaI (New England Biolabs).
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bioRxiv - Genomics 2022Quote: ... Two 500 ng reaction tubes of DLE1-labeled DNA were each mixed with 4 μL of 10X CutSmart buffer (New England Biolabs), 60 μM of lab-made synthetic AdoYnATTO643 (see synthesis in the Supplementary Data) ...
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bioRxiv - Immunology 2022Quote: ... cells were brought to 5×105 cells/ml and incubated with transposition reaction mix (NEBNext High-Fidelity 2X PCR master mix, NEB) for 30 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... These radioactively labeled RNAs were prepared by mixing 1 μM of RNA with 1X of Shrimp Alkaline Phosphatase buffer (rSAP reaction buffer, New England BioLabs), 1 U/μL of rSAP enzyme (New England BioLabs ...
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bioRxiv - Genetics 2022Quote: ... or three (for cloning 4 sgRNAs) fragments were ligated in an equimolar ratio in a Golden Gate reaction with T4 ligase (New England Biolabs) and the BsmBI isoschizomer Esp3I (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We amplified the wild-type EST1 gene from the genome of the haploid strain BY4742 by polymerase chain reaction (PCR) using the high-fidelity Q5 polymerase (NEB) and inserted it into the ΔEST1 cells used in [1] by CRISPR/Cas9 editing ...
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bioRxiv - Genomics 2022Quote: ... CRISPEY-BAR barcodes integrated in the genome were amplified with a first step PCR in 8 tubes of 50 uL reactions using Q5 hot-start DNA polymerase (New England Biolabs) following manufacturer recommendations ...
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bioRxiv - Plant Biology 2022Quote: ... the plasmid was purified and used as template in a single reaction for dsRNA synthesis using the HiScribe T7 High Yield RNA synthesis kit (NEB). For plasmidic DNA removal ...
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bioRxiv - Microbiology 2022Quote: ... and an equimolar mix of primers P5-IR2a-d primer (10 µM) in a reaction with 50 ng of adaptor ligated template and Phusion DNA polymerase (NEB) in a thermocycler with the following program 98°C 3 min ...
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bioRxiv - Microbiology 2022Quote: ... qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (NEB) with the primers listed in (Table S3) ...
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bioRxiv - Plant Biology 2022Quote: ... the sheared cDNA was subject to end-repair and phosphorylation in a single reaction using an enzyme premix (New England Biolabs) containing T4 DNA polymerase ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 μl of the Phire PCR reaction was verified on a 2% agarose gel with a low molecular weight ladder (N3233S, NEB). 15 μl of PCR products were pooled and purified using PCR Purification Kit (D4013 ...
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bioRxiv - Microbiology 2022Quote: ... and BsmBI-v2 (#R0739S) and T7 ligase (#M0318S) were used for the Golden Gate reactions and were obtained from New England Biolabs (NEB). Media components were obtained from BD Bioscience and Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... was added reconstituted with 1 μg of purified recombinant αS (vendor) in 1 μL of 5X RNA Polymerase Reaction Buffer (New England Biolabs) and incubated at 4°C for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Second-strand cDNA was synthesized in a reaction mixture containing Second-Strand Synthesis Kit (New England Biolabs; Ipswich, MA, USA). cDNA was purified using the Qiagen purification kit ...
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bioRxiv - Synthetic Biology 2022Quote: ... The YeastFab assembly is performed according to the reaction system below: 1 μL 10X Buffer for T4 DNA Ligase (NEB), 0.1 μL Purified BSA 100X (Thermo) ...
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bioRxiv - Plant Biology 2022Quote: ... For quantitative PCR 90 ng of template DNA was used in 10 μl qPCR reactions with 0.25 μM primers and Luna qPCR Master Mix (NEB, USA). qPCR was performed in a LightCycler 480 (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) on a QuantStudio 6 Flex thermocycler (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... such that the final concentration of components in the 20 μl PCR were: 1X Q5 Reaction Buffer (New England Biolabs), 1X Q5 High GC Enhancer (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μL of the resuspended pooled oligonucleotide library or NNK-based library was used as an initial reverse primer along with 0.5 μM AAV9_K449R_Forward primer in a 25 μL PCR amplification reaction using Q5 Hot Start High-Fidelity 2X Master Mix (NEB, M0494S). 50 ng of a plasmid containing only AAV9 (K449R ...
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bioRxiv - Immunology 2022Quote: ... The cDNA libraries were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina 96 reactions (New England Biolabs). Libraries were quantified using the KAPA Library Quantification Kit (Roche ...
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bioRxiv - Biochemistry 2022Quote: Drosocin-ribosome complexes were generated by in vitro transcription-translation reactions in PURExpress ΔRF123 in vitro protein synthesis system (New England Biolabs) with the same reaction mix as described earlier in the toeprinting assays ...
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bioRxiv - Genomics 2022Quote: ... The eluted ATAC-Seq library was cloned into AgeI- and SalI-digested pLenti-STARR using a 3:1 molar ratio (insert:backbone) in a total reaction volume of 100 μL using the NEBuilder HIFI DNA Assembly Kit (NEB #E5520S). The reaction was concentrated to a total of 20 μL in a Reaction Cleanup Kit (Qiagen #28206) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The isolated DNA and the initial infectious plasmid were used as DNA template for polymerase chain reaction (PCR) using 30 cycles and the Q5 High Fidelity DNA Polymerase (New England BioLabs) under the recommended conditions by the manufacturer ...
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bioRxiv - Genetics 2022Quote: ... The purified reaction products were pooled at equal molarity and assembled into pCFD5 in a Goldengate reaction using BbsI-HF and T4 Ligase (both New England Biolabs).