Labshake search
Citations for New England Biolabs :
3101 - 3150 of 7437 citations for rno mir 155 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... 500 ng of RNA were retrotranscribed into cDNA using a LunaScript™ RT SuperMix cDNA Synthesis Kit (NEB, Cat. No. E3010L) using the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2024Quote: ... 500 ng total RNA was used as template with the Luna Universal One-step RT-qPCR kit (New England Biolabs, E3005L). For each condition 3 biological replicates with two technical replicate RT-qPCR reactions were run on a CFX96 Connect Real-Time System (BioRad) ...
-
bioRxiv - Genetics 2021Quote: ... The entire alh-6 genomic sequence (ATG to stop) was amplified by PCR and cloned in a linearized pMiniT 2.0 vector (NEB PCR Cloning Kit, Cat. #E1202S). Plasmid DNA was purified using the Zymo Research Zyppy Plasmid Miniprep kit (Cat ...
-
bioRxiv - Molecular Biology 2021Quote: Pooled sgRNA oligonucleotides were synthesized as 76-mers by Custom Array (Bothell, WA, USA) and were amplified by PCR with NEBNext High Fidelity PCR Master Mix (New England BioLabs, NEB, Ipswich, MA, USA) using customized primers (Additional file 2 ...
-
bioRxiv - Cell Biology 2022Quote: ... The V3-V4 hypervariable regions of bacterial 16S rRNA genes were amplified by PCR with Phusion® High-Fidelity PCR MasterMix (New England Biolabs, Ipswich, MA, USA) using specific primer as previously described59 ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... The library was then PCR amplified in five separate aliquots (12 cycles, Phusion High Fidelity® PCR kit, New England Biolabs Inc., Frankfurt, Germany). We used Agencourt® AMPure XP beads (Beckman Coulter ...
-
bioRxiv - Genetics 2022Quote: ... DNA samples were purified before and after PCR with the Monarch® PCR and DNA Clean-Up Kit (New England Biolabs, Ipswitch, MA, USA). Individual sample libraries were pooled ...
-
bioRxiv - Genomics 2021Quote: ... and 7 PCR cycles were used to amplified libraries using NEBnext High-Fidelity 2X PCR Master Mix (New England Biolabs, Ipswich MA, Catalog #M0541S) and SYBR Green I (ThermoFisher ...
-
bioRxiv - Microbiology 2024Quote: ... 5’-GCT GCG TTC TTC ATC GAT GC-3’) were chosen with barcodes for PCR amplification using Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA, USA). The PCR products were then mixed at equal density ratios and purified with a Gel extraction kit (QIAGEN ...
-
bioRxiv - Genomics 2023Quote: ... Almost full-length PCR products were obtained in a preliminary PCR using 1.25U OneTaq® Quick-Load® DNA Polymerase (New England BioLabs, Ipswich, MA, USA), 200 μM mixed dNTPs ...
-
bioRxiv - Plant Biology 2019Quote: ... The PCR products were cloned into pMiniT (New England Biolabs), and the stop codon was altered to create a NheI site using site-directed mutagenesis ...
-
bioRxiv - Synthetic Biology 2021Quote: ... All plasmid-template PCR products were treated with DpnI (NEB) following standard protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... and PCR amplified (8-12 cycles) with Phusion polymerase (NEB) using custom primers (22) ...
-
bioRxiv - Genetics 2020Quote: ... We performed this PCR using Taq DNA Polymerase (M0320S, NEB) and the following conditions ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase (NEB). Each reaction contained 1x Q5 reaction buffer ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and 1X Phusion High Fidelity PCR Mastermix (New England Biolabs). Cycle conditions were 98°C for 30 seconds ...
-
bioRxiv - Molecular Biology 2021Quote: ... PCR was performed using Q5 High-Fidelity DNA polymerase (NEB) while qPCR products were amplified using Ssofast EvaGreen Supermix (BioRad ...
-
bioRxiv - Neuroscience 2021Quote: ... The amplified PCR fragment was subcloned via HIFI cloning (NEB) under a Math1 enhancer.
-
bioRxiv - Molecular Biology 2020Quote: ... PCR was carried out with Phusion polymerase (New England Biolabs), with PCR cycling conditions ...
-
bioRxiv - Molecular Biology 2021Quote: ... Purified PCR product was digested with Hpy188I (New England Biolabs) for 2 hours at 37 °C to generate single dT 3’ overhangs ...
-
bioRxiv - Genomics 2019Quote: ... 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
-
bioRxiv - Genomics 2020Quote: ... PCR mix (NEBNext Ultra II Q5 Master mix, NEB M0544), USER enzyme (NEB M5505) ...
-
bioRxiv - Genomics 2020Quote: ... 12.5 μL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
-
bioRxiv - Developmental Biology 2019Quote: ... purified with the Monarch PCR and DNA Cleanup kit (NEB), and then dialyzed with 0.5X TE buffer using a 0.025 μm VSWP membrane (Millipore ...
-
bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were performed with Q5 polymerase (New England Biolabs) in the buffer provided ...
-
bioRxiv - Cell Biology 2019Quote: ... PCR reactions were performed using Phusion polymerase (New England Biolabs). PCR products were digested with DpnI restriction enzyme (New England Biolabs) ...
-
bioRxiv - Genomics 2019Quote: ... PCR was performed for using Q5 DNA polymerase (NEB #M0491S) with 1X GC buffer (72°C 5min ...
-
bioRxiv - Genomics 2019Quote: ... PCR was performed for using Q5 DNA polymerase (NEB #M0491S) with 1X GC buffer (72°C 5min ...
-
bioRxiv - Plant Biology 2019Quote: ... PCR was conducted using Q5® High Fidelity DNAPolymerase (NEB). DNA fragments were separated in 2% (w/v ...
-
bioRxiv - Microbiology 2020Quote: ... digesting the PCR product with BamHI-HF (New England Biolabs), dephosphorylating with Quick CIP (New England Biolabs) ...
-
bioRxiv - Immunology 2019Quote: ... The PCR product was purified and assembled into BsmBI (NEB) digested vector backbones in a Gibson reaction (NEB) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... PCR reactions were performed using Phusion high HF buffer (NEB) and standard PCR conditions (98°C for 30s ...
-
bioRxiv - Synthetic Biology 2020Quote: ... PCR reactions were performed using Phusion high HF buffer (NEB) and standard PCR conditions (98°C for 30s ...
-
bioRxiv - Microbiology 2020Quote: ... PCRs were performed using Q5 polymerase (New England Biolabs Inc) according to the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Correct PCR products were digested with DpnI (New England Biolabs) to remove the template and subsequently cleaned up with a DNA cleanup kit (Zymo Research ...
-
bioRxiv - Microbiology 2019Quote: PCR was performed using Phusion HF polymerase (New England Biolabs). All restriction enzymes and T4 polynucleotide kinase were purchased from New England Biolabs and used according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2019Quote: ... PCR was performed for using Q5 DNA polymerase (NEB #M0491S) with 1 × GC buffer (72°C 5 minutes ...
-
bioRxiv - Physiology 2021Quote: ... PCR was done using Q5 High-Fidelity DNA polymerase (NEB), PCR primers as listed in Table 1 ...
-
bioRxiv - Genomics 2021Quote: ... 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
-
bioRxiv - Genomics 2021Quote: ... 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added to each well and samples were PCR-amplified (72 °C 5 min ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... DNA absence was confirmed with PCR using Taq polymerase (NEB) with primer 1 & 2.
-
bioRxiv - Genomics 2021Quote: ... Quantitative PCR (qPCR) with the Luna Sybr-Green mastermix (NEB) used primer sequences in Table S3 and 2 μL of a 1:10 dilution of the cDNA ...
-
bioRxiv - Synthetic Biology 2021Quote: ... PCR amplification was performed with Phusion polymerase (New England Biolabs) and TruSeq primers (Sigma) ...
-
bioRxiv - Biochemistry 2021Quote: ... and they were obtained through PCR with Q5 Polymerase (NEB). The PCR products were subjected to 1% agarose gel electrophoresis and they were purified using a Zymogen gel DNA recovery kit (Zymo Research) ...
-
bioRxiv - Neuroscience 2021Quote: ... the PCR product was digested with the AflII enzyme (NEB) whose recognition site is at the targeted cut-off site ...
-
bioRxiv - Cancer Biology 2020Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase (NEB). For reactions using Q5 High-Fidelity DNA Polymerase ...
-
bioRxiv - Cell Biology 2021Quote: ... were cloned using overlap PCR followed by Gibson assembly (NEB) cloning into pHAGE-C-HA/FLAG and were resistant to siRNA # 0 ...
-
bioRxiv - Cell Biology 2021Quote: ... The resulting PCR product was cloned into pMini T2.0 (NEB).
-
bioRxiv - Cell Biology 2021Quote: ... sgRNA sequences were then PCR amplified using Q5 hotstart (NEB) with primers oDF344 (ACACGACGCTCTTCCGATCTtcttgtggaaaggacgaaac ...