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Citations for New England Biolabs :
3051 - 3100 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... we first optimized the wb-WC reaction path in G○U in gas phase using nudged elastic band (NEB) method at B3LYP-D3BJ/def2-TZVP level (Fig ...
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bioRxiv - Biochemistry 2021Quote: ... The reactions were stopped by addition of 2 μl of no SDS-purple gel loading dye (New England BioLabs) or 50% glycerol ...
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bioRxiv - Synthetic Biology 2021Quote: Linear DNA templates for expression in IVTT reactions were created by PCR using Phusion High-Fidelity DNA Polymerase (NEB) with primers pBEST_LinL_F and pBEST_LinL_R (Table S1 ...
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bioRxiv - Molecular Biology 2021Quote: ... washed with 100 μl of 1x MNase Buffer and incubated in 100μl of MNase reaction mix (1x MNase buffer and 30 gel unit/μl MNase (New England Biolabs)) during 3 min at 37°C with mix at 1400rpm ...
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bioRxiv - Genetics 2021Quote: ... The Golden Gate Assembly reaction was set in 200µl PCR tubes (ThermoScientific AB2000) with 2.5 µl 10X T4 DNA ligase buffer (NEB B0202S), 0.5 µl T4 DNA ligase (NEB M0202L) ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μl of this lysate was directly used for PCR reactions with 2× Taq start master mix (M0496L, NEB), and the two NASP clone screening primers (see primer table) ...
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bioRxiv - Biochemistry 2021Quote: ... 0.1 μg cDNA was added to a 50 μl Q5 Hot Start High-Fidelity DNA Polymerase PCR reaction with 0.2 μM each of NEBNext SR Primer for Illumina and NEBNext Index Primer for Illumina (NEB) and run for 6 cycles with a 15 s 62 °C extension step ...
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bioRxiv - Synthetic Biology 2021Quote: Sub-parts (modules, Supplemental Table 1) were amplified via high-fidelity polymerase chain reaction (PCR) (New England Biolabs #M0491S) using dsDNA templates and ssDNA primers listed in Supplemental Table 2 ...
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bioRxiv - Microbiology 2021Quote: ... 20 μl qPCR reactions were prepared in duplicate as follows: 1x Luna Universal Probe qPCR Master Mix (NEB M3004L), 300 nM of forward and reverse primer ...
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bioRxiv - Molecular Biology 2021Quote: ... sequence in a reaction containing 40 ng/μl DNA (4 μg total) and 0.5 U/μl restriction enzyme (HindIII-HF; NEB, Cat ...
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bioRxiv - Microbiology 2020Quote: ... The products of the HiFi reaction were transformed into either NEB10β (for chlamydial transformation plasmids) or NEB5αIq (for BACTH) competent cells (NEB) and plated on appropriate antibiotics ...
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bioRxiv - Molecular Biology 2020Quote: ... the first step PCR reaction was performed in a 12.5 μl mixture containing 1X OneTaq Hot Start Master Mix (NEB), 0.4 μM of each DHPV-U 1538F and DHPV-U 1887R primer and 20 ng of DNA template ...
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bioRxiv - Genomics 2020Quote: ... Capture plate wells contained 5 µl of capture solution (1:500 Phusion High-Fidelity Reaction Buffer, New England Biolabs; 1:250 RnaseOUT Ribonuclease Inhibitor ...
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bioRxiv - Molecular Biology 2021Quote: ... the resulting supernatant was diluted 20-fold and used as template either for a 12.5 uL PCR reaction with Q5 Polymerase (NEB), or for a 10 uL qPCR reaction with the SsoAdvanced Universal SYBR Green 2X Supermix (BioRad) ...
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bioRxiv - Molecular Biology 2021Quote: ... the beads were incubated with 1 µl CK2 enzyme and 1X CK2 reaction buffer (NEB Inc; catalog no. P6010S) supplemented with 200 µM ATP and 30 mM MgCl2 and rotated for 1 h at 30ºC ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions for cloning were performed by using Q5® High-Fidelity DNA Polymerase (New England Biolabs, Inc (NEB). All the products sequenced to verify the absence of any mutations (GATC-biotech).
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bioRxiv - Synthetic Biology 2023Quote: ... Vector DNA was dephosphorylated using quick cow intestinal phosphatase (QCIP) and ligation reactions conducted using T7 DNA ligase (NEB). Purification of DNA products was done by PCR cleanup (E.Z.N.A Cycle Pure Kit ...
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bioRxiv - Systems Biology 2022Quote: ... Restriction digestion was done using specific restriction enzymes BamHI and Hind III (0.5 U final concentration) and 1x cut smart reaction buffer (NEB) incubated in the water bath at 37 °C for 2 hours and 15 minutes respectively followed by heat inactivation for 15-20 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Amplicons for long-range sequencing were generated with Q5 High-Fidelity polymerase in 100 uL reactions (New England Biolabs) including500 nM primers and up to 1000 ng genomic DNA using the following PCR protocol ...
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bioRxiv - Microbiology 2023Quote: ... The 30 μl PCR reaction system contained 15 μl of Phusion high-fidelity PCR Master Mix (New England Biolabs), 0.2 μM forward and reverse primers ...
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bioRxiv - Genetics 2023Quote: ... The PCR reaction was performed using NEBNext® High-Fidelity 2× PCR Master Mix (New England BioLabs, catalog #M0541) with a reaction volume of 10 ul including 5 ul 2× PCR Master Mix ...
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bioRxiv - Molecular Biology 2023Quote: ... in a 10 μL reaction in buffer (50 mM Tris-HCl pH 7.5, 1 mM DTT, 1 U/μL RNase Inhibitor (NEB)) and incubated for 1 hour at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... alkaline phosphatase-treated pBOMBL12CRia(e.v.)::L2 for use in a HiFi reaction according to the manufacturer’s instructions (New England Biolabs (NEB); Cambridge ...
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bioRxiv - Synthetic Biology 2023Quote: ... Reactions were purified according to the ssDNA cleanup protocol from the Monarch PCR cleanup kit (New England Biolabs; T1030L).
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bioRxiv - Genomics 2023Quote: ... Libraries were amplified by PCR for a total of 8 cycles in 100µL reactions with Phusion polymerase (New England Biolabs). No PAGE purification was performed to ensure that our libraries were not biased against short nascent RNA insertions.
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bioRxiv - Cancer Biology 2023Quote: ... PCR amplification was done in a 25µl reaction with 1 unit of NEB Taq DNA polymerase (NEB Catalog# M0273S), 1x Standard Taq Buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatant was removed and chromatin pellets were resuspended in 40 µL 1X DNase I reaction buffer (NEB, Ipswich, MA) containing 1 mM DTT ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was amplified in a 25 µl PCR reactions using 6 µl cDNA and 2X Master mix (NEB). The PCR cycling program was 98 °C for 30 seconds ...
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bioRxiv - Molecular Biology 2023Quote: ... 37°C with rotation) in 100 µL MNase reaction mix (87 µL ddH2O, 10 µL 10x MNase buffer, NEB, 1 µL 100x BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.0-3.0 μg of DNA was used for a ligation reaction in a volume of 1 mL using 1.0 μL of 2000 U/μL T4 DNA Ligase (NEB) for 3C experiments ...
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bioRxiv - Systems Biology 2023Quote: ... The purified linear DNA was used for cloning of pTS040 by Gibson assembly (NEBuilder HiFi DNA Assembly Reaction, NEB) and electroporation of E ...
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bioRxiv - Bioengineering 2023Quote: ... qPCR was performed using 67.5ng of gDNA for each condition in 10µl reactions using Luna Universal qPCR Master Mix (NEB, M3003E).
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bioRxiv - Synthetic Biology 2023Quote: ... and 50 ng of gDNA was used as the template in a 25 µL reaction along with 0.25 µL of Q5 polymerase (NEB), 0.5 µL of 10mM dNTPs ...
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bioRxiv - Synthetic Biology 2023Quote: ... and terminator) was assembled in one-pot Type IIS DNA assembly reaction using BsaI-HFv2 (New England Biolabs, NEB). Transcription units to assay the sensor output contained the PQS promoter expressing the gfp gene ...
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bioRxiv - Synthetic Biology 2023Quote: ... and terminator) was assembled in one-pot Type IIS DNA assembly reaction using BsaI-HFv2 (New England Biolabs, NEB). Transcription units to assay the sensor output contained the PQS promoter expressing the gfp gene ...
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bioRxiv - Pathology 2023Quote: ... Each 10 µL reaction contained Luna Probe One-Step RT-qPCR 4X Mix (New England Biolabs, Ipswich, MA, USA), primers (10 µM) ...
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bioRxiv - Microbiology 2023Quote: ... We incubated TXTL reactions at 29 °C for 6 h and digested at 37 °C with PacI (NEB R0547S) as instructed by the provider and added water instead of PacI for the undigested control ...
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bioRxiv - Plant Biology 2023Quote: ... the SQK-RNA002 kit (Oxford Nanopore Technologies) was used together with NEBNext® Quick Ligation Reaction Buffer (NEB B6058), T4 DNA Ligase 2M U/ml (NEB M0202) ...
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bioRxiv - Molecular Biology 2022Quote: ... The total isolated gDNA was processed in batches of 5 µg per PCR reaction with Q5 polymerase (NEB M0491L). One PCR reaction contained 10 µl 5x reaction buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... Genotyping PCR reactions (36 amplification cycles) were performed using Taq DNA Polymerase with ThermoPol Buffer (New England Biolabs, M0267L), dNTPs from Promega (U1511 ...
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bioRxiv - Cell Biology 2024Quote: ... or else generated by polymerase chain reaction or gBlock synthesis (IDT) and assembled using HiFi assembly (New England Biolabs) according to the manufacturer’s instructions as shown in Table 1 ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR reaction was mixed by combining 25 μL of Q5 High-Fidelity DNA Polymerase (New England Biolabs #M0494S), 2 μL of the oligo pool solution ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were amplified using inner primers ACL-P1060 and ACL-P1061 in a 40μl PCR reaction using Q5 Hot Start 2x Master Mix (New England Biolabs) for 20-25 cycles at an annealing temperature of 64°C ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared by on-bead reactions using the NEB Next Ultra II DNA Library Preparation Kit (NEB, E7645S). The beads were separated on a magnetic stand ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR reactions were performed using Phusion® high-fidelity DNA polymerase according to the manufacturer’s protocol (New Englang BioLabs). Amplified flanking recombination regions were merged with the MX cassette through Circular Polymerase Exchange Cloning into the pUC19 plasmid [67] ...
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bioRxiv - Genomics 2024Quote: For a typical blunting reaction 5-50 ng of cfDNA are mixed with 5 μl of CutSmart 10x (NEB), 5 μl of dNTPs 1mM each ...
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bioRxiv - Microbiology 2024Quote: ... 30 µL of the barcoded DNA was ligated onto 2.5 µL Native Ligation Adapter (NA, ONT SQK-NBD114-96) by addition of 12.5 µL NEBNext Quick Ligation Reaction Buffer (NEB) and 5 µL high conc ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of isolated phage T4 in Pi-Mg buffer was used as a PCR template in the first PCR with the following reaction mix: 0.125 µM each primer (Supplementary Table 2) in 1x High Fidelity Master Mix (NEB) with a total volume of 10 µl ...
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bioRxiv - Biochemistry 2023Quote: ... in a total reaction volume of 100 µL consisting of 50 units of T4 RNA ligase 1 (NEB #M0204S), 1mM ATP ...
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bioRxiv - Cancer Biology 2023Quote: ... a Poly(A) tailing reaction was performed for 1 hour according to the HiScribe T7 ARCA manual (NEB, E2060S). Subsequently ...