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Citations for New England Biolabs :
3151 - 3200 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Reactions were incubated at 37°C for 3 hours then digested with 64 units of RNase Free DNaseI (NEB) at 37°C for 15 minutes ...
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bioRxiv - Genomics 2024Quote: ... a 16 hour RCA reaction was performed at 30°C using a mixture containing 500 µM dNTP (NEB #N0447S), 50 µM amino-allyl-dUTP (Thermo Scientific #R1091) ...
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bioRxiv - Genetics 2024Quote: ... All reactions were performed in a sterile Clean Prep Hood using NanoDrop 1000 and SandWatch software (New England Biolabs) and 250ng input RNA in a 20ul reaction according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... All polymerase chain reactions (PCRs) were performed with the respective primers using the Phusion High-Fidelity DNA Polymerase (NEB).
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bioRxiv - Synthetic Biology 2024Quote: ... and a reaction mixture was set with the Gibson Assembly Master Mix by following the supplier’s instructions (New England BioLabs). The reaction was carried out in a thermocycler at 50°C for 15 min ...
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bioRxiv - Biochemistry 2024Quote: ... 500 fmol of each plasmids were mixed in a total of a 10 µL reaction of 1x Cutsmart (NEB) and with 1 µL PmeI (NEB #R0560) ...
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bioRxiv - Biochemistry 2024Quote: ... 25 fmol of each plasmid was added per reaction and assembled with NEBridge® Ligase Master Mix (NEB #M1100) and BasI-HF-V2 (NEB #R3733 ...
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bioRxiv - Cell Biology 2024Quote: ... all extracted gDNA was used in 100ul PCR reactions of NEBNext Ultra II Q5 Master Mix (NEB Cat M0544X) using 10ug of gDNA as template and 22 cycles of exponential amplification ...
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bioRxiv - Cell Biology 2024Quote: ... three DNA fragments were generated by polymerase chain-reaction (PCR) using Q5 High-Fidelity DNA Polymerase (New England Biolabs). The first fragment (445 bp) ...
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bioRxiv - Genetics 2020Quote: Total RNA was extracted using the standard hot acid phenol method and treated with DNase 1 (New England Biolabs). qRT-PCR was performed using amfiRivert cDNA synthesis Platinum Master Mix from Gendepot ...
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bioRxiv - Biochemistry 2021Quote: ... The alkyne-PAR was purified to remove excess alkyne-PEG1-amine using the Monarch Nucleic Acid Cleanup Kit (NEB) following the recommended protocol ...
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bioRxiv - Biochemistry 2021Quote: ... 70 and 80 amino acids deletion mutants were carried out with the Q5 mutagenesis kit (New England Biolabs, MA). All AtAtm3 constructs were overexpressed in Escherichia coli BL21-gold (DE3 ...
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bioRxiv - Biochemistry 2023Quote: ... Single or multiple amino acid mutations in full-length GPR161 were generated using Q5 site-directed mutagenesis kit (NEB).
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bioRxiv - Biophysics 2023Quote: ... Single-amino acid exchanges were generated by Q5® site-directed mutagenesis (New England Biolabs GmbH, Frankfurt am Main) using the appropriate primer pairs which were designed by using NEBaseChanger ...
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bioRxiv - Microbiology 2023Quote: 20 ng of nucleic acid was enriched for viral genomic sequences using NEBNext Microbiome DNA Enrichment Kit (NEB, E2612) to reduce host genomic DNA contamination ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA was purified from single-humanized Hsα1 and quintuple-humanized Hsα1,α2,α3,α4,α7 strains using the Monarch Nucleic acid purification kit according to the manufacturer’s protocol (NEB). Spheroplasts were obtained before the genomic DNA extraction for a high-quality DNA prep ...
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bioRxiv - Biochemistry 2023Quote: ... The extracted plasmid DNA was reconstituted in cacodylic acid (10 mM, pH 6.5) and subjected to linearization by HINDIII-HF restriction digestion (New England Biolabs). Following digestion ...
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bioRxiv - Microbiology 2024Quote: ... purified nucleic acids were converted to complementary DNA (cDNA) while random primer mix (New England Biolabs, Ipswich, MA, USA) was used to prepare double-stranded cDNA using NEBNext Non-Directional RNA Second Strand Synthesis module ...
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bioRxiv - Plant Biology 2021Quote: ... The final library products were further purified using an 8% polyacrylamide gel to excise 130-160nt products relative to the pBR322 DNA-MspI Digest ladder (New England Biolabs, Cat# E7323AA). The purified libraries were pooled and sequenced (single end 50 bp ...
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bioRxiv - Genomics 2020Quote: ... The PCR products were cloned in to the pRF-HU2 vector system 70 using USER enzyme following manufactured protocol (New England Biolabs, MA, USA). Correctly ligated vector was transformed into Agrobacterium tumefaciens strain AGL1 used for V ...
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bioRxiv - Genetics 2021Quote: ... the poly (A) product was broken into pieces at 94 °C for 5-7 min using the Magnesium RNA Fragmentation Module (NEB, MA, USA). The RNA fragments were then reverse-transcribed using the SuperScript™ II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Around 1 µg of each PCR product was used as template for dsRNA synthesis using the HiScribe T7 High Yield RNA synthesis kit (New England BioLabs, Cat # E2040S) as per the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... Cloning of PCR products and gBlocks in the pKSB- intermediate vector 43 was performed using NEBuilder® HiFi DNA Assembly (New England Biolabs, France) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Fragmentation and library construction of the resulting PCR products were performed using an NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the DNA products from each ChIP assay were first end repaired with end repair enzyme mix (New England Biolabs, Inc., Cat#M6630), then ligated to NEBNext adaptor included in the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (Cat#E7645L ...
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bioRxiv - Plant Biology 2021Quote: ... The digestion products were run on agarose gel and purified with Monarch® DNA Gel Extraction Kit (New England Biolabs® Inc.). The asRNA genes were ligated into pLSU-1.1 with T4 DNA Ligase (New England Biolabs® Inc. ...
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Mitochondrial mutations in Caenorhabditis elegans show signatures of oxidative damage and an AT-biasbioRxiv - Genetics 2021Quote: ... The adaptor-ligated product was treated with a cocktail of three repair enzymes (Uracil-DNA Glycosylase, Fpg, and Endonuclease III in NEB CutSmart Buffer) to remove fragments with single-stranded damage and 16 ng of repaired DNA was used as input for the first round of PCR (13 cycles) ...
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bioRxiv - Genomics 2022Quote: ... The amplified products were fused and introduced into pFC332 using Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix, New England Biolabs, MA, USA). The vectors containing the sgRNA were then transformed into competent Escherichia coli TOP10 cells for multiplication overnight ...
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bioRxiv - Systems Biology 2022Quote: ... total RNA libraries were built using the NEBNext Ultra II library prep kit for Illumina (New England BioLabs inc., product code: E7775) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The mCherry cassette was cloned using G-block double-stranded DNA fragments synthesized by Integrated DNA Technologies (IDT) and PCR products generated using Q5 DNA polymerase (New England Biolabs, Cat#: M0492). The mCherry coding sequence was synthesized with silent mutations ablating potential splice donor and splice acceptor sites that could interfere with intended splicing of the intron ...
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bioRxiv - Developmental Biology 2022Quote: ... the PCR products (primers shown in Table S1) were digested with AvaII and HpyCH4IV restriction enzymes (New England BioLabs; Ipswich, MA, USA), respectively ...
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bioRxiv - Molecular Biology 2020Quote: Thermocycler was used to denature and anneal 5 µl of PCR product as recommended by manufacturer (EnGen™ Mutation Detection Kit, NEB, E3321S). Thermocycler was adjusted for heteroduplex formation as following ...
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bioRxiv - Genomics 2020Quote: ... and 10-12 day adult TRAP libraries were prepared with the NEBNext Ultra RNA library Prep Kit for Illumina (NEB, product E7530S). ChIP-seq libraries were prepared using the NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (NEB ...
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bioRxiv - Genomics 2020Quote: The isolated total RNA for TRAP libraries underwent poly(A)+ selection using the NEBNext Poly(A) mRNA isolation module (NEB, product E7490S). 48hr APF TRAP libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Membranes were incubated overnight at 4 °C with the appropriate antibody: rabbit monoclonal anti-GSK3β (1:1,000; product # 9315, Cell Signalling Technology, New England BioLabs, Whitby, Ontario, Canada), rabbit polyclonal anti-p[Ser9]GSK3β (1:500 ...
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bioRxiv - Genetics 2021Quote: PCR products (150 – 200 ng) were digested for 30 min at 37°C in 20 ul 1X restriction enzyme buffer (NEB buffer v3.1) containing 500 ng Cas9 (Alt-R® S.p ...
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bioRxiv - Cell Biology 2020Quote: ... primers 1 - 3) and then inserting the resulting PCR product into BamHI-digested pBMN-mCherry using Gibson assembly (New England Biolabs, Ipswich, MA). The resulting pBMN-ARHGAP36-mCherry vectors with XhoI and SacII restriction sites were subsequently used in all experiments described herein.
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bioRxiv - Biochemistry 2020Quote: ... Excess iodoacetamide was quenched with DTT and the samples were digested overnight with endoproteinase GluC (1:100) at room temperature (New England Biolabs, Product #P8100S). Digested peptides were dried in a vacuum concentrator ...
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ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriersbioRxiv - Cell Biology 2021Quote: ... Gel purified PCR product soo-XbaI-Gateway-XbaI-soo was then cloned into pTGA linearized by XbaI digestion (New England Biolabs, cat#R0145S) using SoSoo mix reagent (TreliefTM SoSoo Cloning Kit ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were visualized on a 1% agarose gel using the Quick-load Purple 1-Kb DNA ladder (New England Biolabs, Ipswich, MA). Primer3 Plus (Untergasser et al. ...
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bioRxiv - Immunology 2020Quote: ... The heavy and light chain PCR products were cloned in frame with seamless cloning using the NEBuilder® HiFi DNA Assembly Master Mix (NEB, UK) after linearizing the vectors with KpnI (5’ ...
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bioRxiv - Immunology 2023Quote: ... The multiplex products were used as templates to the second PCR using Phusion High-Fidelity DNA Polymerase (New England Biolabs Inc., M0530L) that barcodes each well/perturbation separately ...
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bioRxiv - Genomics 2023Quote: ... The PCR2 product pools were subjected to three steps of purification with the Monarch PCR & DNA Cleanup Kit (New England Biolabs, no. T1030L), Gel DNA Recover Kit (Zymoclean ...
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bioRxiv - Microbiology 2023Quote: ... purified and subjected to ligation with the digested and gel-purified PCR product utilizing T4 DNA ligase (New England Biolabs, Cat. # M0202S).
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bioRxiv - Cell Biology 2022Quote: ... PRR14 1-212 PCR products were sub-cloned into the expression vector pCMV6-AN-mGFP using T7 ligase (New England Biolabs, cat# M0318S) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... The libraries were then run on a 3% agarose gel and the product was extracted using NEB Monarch DNA Gel Extraction Kit (NEB, Cat# T1020L). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... 173 bp TBR-PCR target products were excised and purified using an Exo-CIP Rapid PCR Cleanup Kit (New England Biolabs, Ipswich, USA) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... total RNA libraries were built using the NEBNext Ultra II library prep kit for Illumina (New England BioLabs inc., product code: E7775) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was incorporated into a digested pFLP2 backbone using KpnI and BamHI restriction sites and HiFi ligation mix (NEB, Ipswich, MA). All restriction enzymes are from NEB (Ipswich ...
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bioRxiv - Immunology 2022Quote: ... and unc-54 3’utr pieces were Gibson assembled using the NEBuilder assembly kit and the assembled product was then PCR-amplified with Phusion DNA polymerase (New England Biolabs, Ipswich, MA). The PCR-amplified assembly was A-tailed for 30 min at 70 °C ...