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Citations for New England Biolabs :
3101 - 3150 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... The ligation reaction was performed with 25ng of digested vector and 3.8ng of inserts using T4 DNA ligase (NEB) at 16°C for 15 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... The reactions were performed in triplicate for 14 cycles using Q5 Hot Start High-Fidelity 2X Master Mix (NEB), pooled and purified using AMPure XP beads (Beckman Coulter).
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bioRxiv - Neuroscience 2023Quote: ... DNA ligation was performed in the same reaction mixture containing the phosphorylated samples using 5U T4 DNA ligase (NEB). A transformation assay was performed using 3 μL DNA ligation product in DH5alpha bacterial cells and the positive clones were verified by Sanger sequencing.
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bioRxiv - Neuroscience 2023Quote: ... These two amplicons were incubated with SfoI digested pET23a-His-TEV plasmid in a HiFi Assembly reaction (NEB, E2621S) to generate a plasmid where HaloTag and SpyCatcher3 is linked with a GSGESGSG linker sequence.
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bioRxiv - Cell Biology 2024Quote: ... 16.4 mL elution was taken into 40 mL ligation reaction (13 Quick ligase buffer [NEB], 4000U Quick ligase [NEB] ...
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bioRxiv - Microbiology 2023Quote: ... several 50 μl PCR reactions were run using these oligos and Q5 or Phusion polymerase (New England Biolabs, USA). A sample was run on a 1% agarose gel and imaged via UV transilluminator ...
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bioRxiv - Molecular Biology 2023Quote: ... the circular fragments were amplified by two rounds of polymerase chain reaction (PCR) with Q5-High Fidelity polymerase (NEB). Both PCR rounds begin with an initial denaturation at 98 °C for 30 s ...
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bioRxiv - Biochemistry 2023Quote: 3 μL of the transcription and co-transcriptional reactions were added to a 6 μL mixture containing 1x DNase I reaction buffer and 0.5 U/μL DNase I (New England Biolabs). After incubation at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... 16 reactions were carried out per amplicon per cell line using Q5 High-Fidelity 2x master mix (NEB, #M0492L) with primers containing partial Illumina adapters ...
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bioRxiv - Bioengineering 2023Quote: ... 20 ng of each integrase amplicon was used per reaction using PURExpress® In Vitro Protein Synthesis Kit (NEB) in a total volume of 9 µl ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... The quantitative PCR reaction mix contained 5 µl of the Luna Universal qPCR Master Mix (2X, New England BioLabs), 1 µl of the forward primer (10 µM) ...
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bioRxiv - Microbiology 2023Quote: A 10 µL reaction containing 200 ng of pTrc200HA plasmid DNA and 1× CutSmart Buffer (New England BioLabs, USA) with partially purified V2c or its variants normalized by the major V2c protein band was incubated at 37°C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Reactions were set up as per manufacturer’s instructions with the addition of 4 units of Murine RNase Inhibitor (NEB) and 5% DMSO ...
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bioRxiv - Microbiology 2022Quote: ... A volume of ultrapure water needed to make the reaction up to 37.5μL after the addition of rCutsmart (NEB B6004S) and PspGI (NEB R0611 ...
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bioRxiv - Molecular Biology 2022Quote: ... The RT reaction was then amplified using PHUSION® high-fidelity DNA polymerase using HF buffer (New England Biolabs) via PCR to generate a 1675-bp amplicon ...
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bioRxiv - Biophysics 2022Quote: ... After the IVT reaction was complete the DNA template was digested using DNase 1 enzyme (New England Biolabs, UK). The reaction mixture was purified ...
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bioRxiv - Evolutionary Biology 2022Quote: 75ng of genomic DNA were used for PCR amplification in two 50µl reactions with 25µl 2xQ5 mix (NEB M0492), 2.5µM of each forward (FOS ...
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bioRxiv - Molecular Biology 2023Quote: ... ddH2O and 2μL of diluted cDNA were used in 10μL qPCR reactions containing the Luna® Universal qPCR Master Mix (NEB) and 0.3μM of gene-specific forward and reverse primer ...
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bioRxiv - Bioengineering 2023Quote: ... reactions were carried out in a total of 25 μL containing 1 X Isothermal Amplification Buffer (New England Biolabs), 5 mM MgSO4 ...
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bioRxiv - Genomics 2022Quote: ... We then amplified barcodes from both cDNA and DNA in 4 PCR reactions per sample using Q5 (NEB #M0492) and primers specific to the reporter genes (GWLP P3 ...
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bioRxiv - Genomics 2022Quote: ... OMNI ATAC-Seq was performed as previously described with 320,000 human CD4+ T cells (in total) in batches of 100,000 cells per reaction (NEB #M0544S).(26 ...
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bioRxiv - Genetics 2023Quote: ... 100 ng of genomic DNA was digested with 5U of BtsCI in a 3 μl reaction (New England Biolabs), 1 hour at 50°C incubation and no enzyme denaturation ...
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bioRxiv - Genomics 2023Quote: ... The reaction was inactivated at 25 °C for 10 min with 1.25 μL of Murine RNAse inhibitor (New England Biolabs).
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bioRxiv - Genomics 2023Quote: ... All PCR reactions were performed with 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 μM of forward and reverse primers ...
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bioRxiv - Bioengineering 2023Quote: ... previously described 34,51 containing the tetracycline resistance cassette and sacB3 gene via a three-fragment HiFi reaction (New England Biolabs). Escherichia coli S17-1 was transformed with the pMTL70641:ΔpilA construct and conjugated with C ...
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bioRxiv - Genetics 2023Quote: ... Input genomic DNA was first amplified in a 10μL reaction for 30 cycles using NEBNext High-Fidelity 2×PCR Master Mix (NEB). Amplicons were purified using AMPure XP beads (Beckman Coulter ...
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bioRxiv - Biophysics 2023Quote: ... and used in equimolar amounts in a Gibson assembly reaction with NEBuilder HiFi DNA Assembly Master mix (NEB # E2621S). The reaction was incubated at 50°C for 60 min and directly transformed into chemically competent NEB 10-beta E ...
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bioRxiv - Genetics 2023Quote: ... digested pJFRC12-10XUAS-IVS-myr::GFP backbone using the following reactions conditions: 4 µL T4 ligase buffer (10x) (NEB), 20 µl plasmid backbone DNA (0.005 pmol) ...
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bioRxiv - Plant Biology 2024Quote: ... 0.81 nmol LbCas12U protein and 0.45 nmol of each of three crRNAs were assembled in 1 x Nuclease Reaction Buffer (NEB). Protein and crRNAs were mixed and incubated for 10 min at room temperature ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Each plate’s DNA library was then amplified in two 50 µl Q5 High-Fidelity DNA Polymerase reactions (New England BioLabs) for 11 thermocycles ...
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bioRxiv - Synthetic Biology 2024Quote: ... All reactions were then digested with 1 µl ExoI and purified using the Monarch DNA Gel Extraction Kit (NEB), eluting in 10 µl of elution buffer (10 mM Tris•Cl pH 7.4) ...
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bioRxiv - Plant Biology 2024Quote: ... pDGE9 and pDGE11 respectively45 via a Golden Gate reaction with the type-IIS restriction endonuclease BpiI (New England Biolabs). These were then simultaneously transferred into a single vector via a Golden Gate level 1 reaction with BsaI (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... Reactions were then DpnI-treated for 6 hours and purified using the Monarch PCR and DNA Cleanup Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... we used 2.5 μg of the DNA in a 50 μl volume reaction and used Cre enzyme (2 units/μl) (NEB). This was incubated at 37°C for 30 minutes and then the enzyme heat-inactivated at 70°C for 10 minutes ...
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bioRxiv - Genomics 2024Quote: ... All the ligation products were used as templates for RT in a final 30 μl reaction volume containing 8 U of Bst 3.0 DNA Polymerase (8 U/μL, NEB), 1X RT reaction buffer ...
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bioRxiv - Microbiology 2024Quote: ... All PCR reactions were completed using Q5® Hot Start High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA) following the manufacture’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Ligation reactions were carried out overnight at 16°C using 1µl of T4 DNA Ligase (20 U/µl; NEB). Plasmids were then cloned in E ...
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bioRxiv - Microbiology 2024Quote: ... All primers are shown in Table S5 and all PCR reactions were performed using Q5 High-Fidelity polymerase (NEB). HIV-1NL4-3 (pHIV-WT ...
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bioRxiv - Genomics 2024Quote: ... Each sample were subjected to one reaction of NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645). Deamination reactions were conducted as described before ...
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bioRxiv - Genomics 2024Quote: ... a 200 µL indexing PCR reaction was prepared with 100 µL of NEBNext High-Fidelity 2X Master Mix (NEB), 90 µL of template DNA (SPRI purified from the previous section) ...
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bioRxiv - Genomics 2024Quote: A 30 µL indexing PCR reaction was prepared with 15 µL of NEBNext High-Fidelity 2X Master Mix (NEB), 10 µL of template DNA (SPRI purified from the previous section) ...
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bioRxiv - Molecular Biology 2024Quote: ... Three independent transformations of the Gibson Assembly reactions were carried out using NEB 10-beta electrocompetent E.coli (NEB #C3020K) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmid pEGFP-C1 was used as a template in a PCR reaction using Q5 DNA polymerase (New England Biolabs) and oligonucleotides (5’-ACGCGTAAATTGTAAGCG-3’ and 5’-AACAACAACAATTGCATTCATTTTATG-3’ ...
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bioRxiv - Synthetic Biology 2024Quote: ... For USER assembly 0.1 to 0.5 pmol fragments were added in an equimolar ratio in a 10 μl reaction that included 0.75 µL USER enzyme (Uracil-DNA Glycosylase and DNA-glycosylase-lyase Endonuclease VIII; New England Biolabs), 1ul rCutSmart Buffer (50 mM potassium acetate ...
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bioRxiv - Systems Biology 2024Quote: ... and close circular plasmids were assembled via a single restriction-ligation reaction with BsaI enzyme (R3535L, New England Biolabs) and T4 DNA ligase (M0202L ...
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bioRxiv - Microbiology 2024Quote: ... part of the ONT Kit) to the RNA sample using a mix of 3μl NEBNext Quick Ligation Reaction Buffer (New England BioLabs), 0.5μl RNA CS (ONT Kit) ...
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bioRxiv - Microbiology 2024Quote: ... Both PCR reactions used 0.02U/L Q5 Hot Start high-fidelity DNA polymerase (New England BioLabs, Ipswich, MA, USA) with 200M dNTPs and 0.5M forward and reverse primers in 1M Q5 reaction buffer ...
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bioRxiv - Neuroscience 2024Quote: ... DNA contained in the eluate was then amplified for 14 cycles in 25μl PCR reactions using NEBNext High-Fidelity Q5 2X PCR Master Mix (NEB) and 0.5 mM each of Solexa 1GA and Solexa 1GB primers ...
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bioRxiv - Neuroscience 2024Quote: ... For sexing of pups we added 1µL DNA to a reaction with the Q5 Master Mix (NEB, cat# M04925) and primer sets for amplification of the IL3 (For 5’-GGG ACT CCA AGC TTC AAT CA-3’ ...
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bioRxiv - Neuroscience 2024Quote: ... + 7.5 µL of 10X NEB DNase I Reaction Buffer (Cat. No. B0303S; New England BioLabs® Inc., Ipswich, MA) was added to the silica resin bed of the column ...