Labshake search
Citations for New England Biolabs :
2601 - 2650 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... The whole resulting product was then PCR-amplified using indexed primers with NEBNext High-Fidelity 2X PCR Master Mix (NEB). First ...
-
bioRxiv - Genomics 2020Quote: ... was transcribed from a sgRNA-coding PCR product with a 5′ T7 promoter sequence using HiScibe T7 Quick High yield RNA Synthesis kit (NEB). The transcription was performed at 37 °C overnight and then purified by phenol ...
-
bioRxiv - Synthetic Biology 2020Quote: ... All digested products were column purified and mixed at a 6:1 molar ratio in the presence of T4 DNA ligase (NEB).
-
bioRxiv - Evolutionary Biology 2021Quote: ... The PCR product and the plasmid pT1-3B (42) was digested with the restriction enzymes Sal1 and HindIII (New England Biolab (NEB)) ...
-
bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB ...
-
bioRxiv - Biochemistry 2020Quote: ... 20 pmol of the purified product was then incubated with 25 pmol of a complementary primer in 1X T4 DNA ligase buffer (NEB) supplemented with 40 μM dNTPs ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Genetic parts were amplified using primers listed in Table S1 and PCR products were purified with the Monarch PCR & DNA Clean up kit (NEB). Parts were assembled into plasmid constructs mainly by Gibson assembly [41] using the isothermal method ...
-
bioRxiv - Neuroscience 2021Quote: ... The Purified PCR product was treated with Cre recombinase as described in the instructions (M0298, New England Biolabs, Ipswich, MA). The CaMK1 floxed PCR product and control plasmid (pLOX ...
-
bioRxiv - Bioengineering 2021Quote: ... 1 µL linearized expression plasmid was assembled with 3 µL each of PCR amplified product using 4 µL of NEBuilder HiFi DNA Assembly MasterMix (#E2621L, New England Biolabs) in a 96-well plate format ...
-
bioRxiv - Neuroscience 2021Quote: ... genomic PCR products containing the target sites of selected gRNAs were incubated with SpCas9 protein (New England Biolabs, Ipswich, MA) following the manufacturer’s protocol and analyzed on 2% agarose gel stained with ethidium bromide ...
-
bioRxiv - Microbiology 2021Quote: ... difficile strain 630 genomic DNA and purified PCR products were directly cloned into the PmeI site of pMSR vector using NEBuilder HiFi DNA Assembly (New England Biolabs). All pMSR-derived plasmids were initially transformed into E ...
-
bioRxiv - Biochemistry 2021Quote: ... The guide RNA target site was amplified from transfected cells and analyzed by T7 Endonuclease I digestion of re-annealed PCR products (New England Biolabs). The guide RNA eventually selected for genome editing in embryos was Bahd1-sg47B (protospacer sequence 5’-GCCTGTAATACCACAGG −3’) ...
-
bioRxiv - Biochemistry 2020Quote: ... the truncated CNNM2 PCR products were cloned into the pCINE-IRES-GFP vector by digestion with restriction enzymes NheI (New England Biolabs) and XhoI (New England Biolabs).
-
bioRxiv - Bioengineering 2021Quote: ... PCR products were gel-purified and used for a second round of PCR amplification (Q5 NEB master mix, 7 cycles) using custom primers to attach Illumina read sequences ...
-
bioRxiv - Microbiology 2020Quote: ... Obtained PCR product (531 bp) and pRSF-NT vector were digested with NcoI-HF and HindIII-HF (New England BioLabs) and ligated using T4 DNA ligase (New England BioLabs) ...
-
bioRxiv - Microbiology 2021Quote: ... The PCR product was ligated to a low-copy plasmid vector pWSK29 (BamHI/Sall) by Gibson assembly (New England Biolabs). Similarly ...
-
bioRxiv - Microbiology 2021Quote: ... The resulting PCR products were inserted into pSN054 cut with FseI and I-SceI respectively via Gibson Assembly (New England Biolabs). The recodonized Pf3D7_1437000 gene was synthesized by GENEWIZ (South Plainfield ...
-
bioRxiv - Microbiology 2021Quote: ... The three PCR products were assembled as one large fragment (5’ cynX - insert - lacA3’) by Gibson Assembly (New England Biolabs). The assembled DNA was transformed into electrocompetent WT E ...
-
bioRxiv - Microbiology 2021Quote: ... was digested with NotI and NheI and the mgrB PCR product was cloned using the NEBuilder HiFi DNA assembly kit (NEB), followed by transformation into the E ...
-
bioRxiv - Microbiology 2021Quote: ... The product was then transcribed to crRNA using HiScribe T7 Quick High Yield RNA Synthesis kit (New England Biolabs; E2050S) incubating at 37°C overnight ...
-
bioRxiv - Microbiology 2021Quote: ... The PCR product was cloned into NdeI-SapI site of the expression vector pTXB-1 (Table 1, New England Biolabs) with a C-terminally tagged chitin binding domain (CBD ...
-
bioRxiv - Microbiology 2020Quote: ... and the amplified product was cloned into the PCR-generated pCjSpLe94(29) by NEBuilder HiFi DNA Assembly cloning (New England BioLabs), generating pNKLiG1 ...
-
bioRxiv - Biochemistry 2021Quote: All plasmids were constructed by Gibson Assembly from PCR products generated using Q5 Hot Start DNA Polymerase (New England Biolabs) or Phusion Polymerase (generated in-house) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Purified PCR product was inserted into pAIP backbone using HpaI and EcoRI restriction endonucleases (New England Biolabs; Euroclone, Milan, Italy). Correct cloning was checked by restriction analysis and direct sequencing (Eurofins Genomics).
-
bioRxiv - Cell Biology 2021Quote: ... The PCR product was assembled into pFA6a-GFP-his3MX6 (Longtine et al., 1998) digested with SalI and PacI (New England BioLabs) using the Gibson Assembly Master Mix (New England BioLabs).
-
bioRxiv - Bioengineering 2022Quote: ... Linearized plasmids were used as templates and RNA products were then produced using the HiScribe T7 RNA Synthesis Kit (NEB) per manufacturer’s protocol.
-
bioRxiv - Microbiology 2022Quote: ... Later steps included purification of the PCR products followed by restriction with the restriction enzymes AvrII and NotI (NEB, UK) and ligation with the T4 DNA ligase (Promega ...
-
bioRxiv - Bioengineering 2022Quote: ... In vitro Cas9 cleavage assay was carried out using purified IVT product and Cas9 protein (Cas9 Nuclease, S. pyogenes, New England BioLabs) following manufacturer’s instructions ...
-
bioRxiv - Physiology 2022Quote: ... while an additional step which added 3’ A-overhangs to the slc15a2a purified PCR product was performed using Taq DNA polymerase (New England Biolabs) before cloning into pCR4-TOPO vector (Thermo Fisher Scientific) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Digested PCR products were then ligated to the digested pcs2+MCS-P2A-sfGFP plasmid using T4 DNA ligase (M0202S, NEB) in a 3:1 insert:backbone ratio ...
-
bioRxiv - Microbiology 2022Quote: ... The ORF was removed by inverse PCR to generate the genomic deletion vector (P5/P6) and plasmid recircularized from PCR product (KLD enzyme blend, NEB).
-
bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was gel-purified and assembled into an NdeI-HindIII-cut pET21b using a 2x Gibson master mix (NEB). Gibson assembly was possible owing to a 23-bp sequence shared between the NdeI-and-HindIII cut pET21b backbone and the PCR amplified fragment ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... We created a single library by pooling these products after dual barcoding using Nextera N5/N7 primers and OneTaq Polymerase (New England Biolabs). We then cleaned the library using Ampure XP (Beckman Coulter ...
-
bioRxiv - Bioengineering 2022Quote: ... the two primers which contain the gRNA and have 4 nt overhang at 5’-end were annealed (3) the annealing product was ligated with PaqC I (New England Biolabs)-digested pMM002P or pMM005 ...
-
bioRxiv - Immunology 2022Quote: Fragmentasion and adaptor ligation were performed using 10-20ng of second-PCR product as a template with NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs) with some modifications ...
-
bioRxiv - Microbiology 2020Quote: ... This amplification product and the vector were cleaved by NsiI and ligated using T4 DNA ligase (New England BioLabs, Inc.). Insert orientation was checked by sequencing ...
-
bioRxiv - Developmental Biology 2021Quote: ... Reaction products were used as a template for the in vitro transcription (HiScribe T7 High Yield RNA Synthesis Kit, England Biolabs) and purified ...
-
bioRxiv - Microbiology 2020Quote: ... A 1.5kb product for STM2441 including the native promoter was generated from template genomic DNA using Q5 DNA polymerase (New England Biolabs) with an annealing temperature of 72°C and an extension time of 40s for 35 cycles ...
-
bioRxiv - Microbiology 2020Quote: ... Ligation of the product and linearized vector was performed overnight at 14°C with T4 DNA ligase (New England Biolabs). The resulting construct was transformed into DH5α Escherichia coli by heat shock and transformants were obtained by selection on LB agar with carbenicillin and X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside ...
-
bioRxiv - Molecular Biology 2020Quote: ... were engineered to lack the guide sequence and were assembled from PCR products by Gibson assembly (Gibson Assembly Master Mix kit, New England Biolabs).
-
bioRxiv - Molecular Biology 2020Quote: ... The PCR product was then cloned into pTH19 linearized with BamHI and HindIII using the NEBbuilder assembly kit (New England Biolabs). The plasmid was transformed into yeast as described previously 71 selecting for transformants on media lacking uracil.
-
bioRxiv - Microbiology 2020Quote: ... RT-PCR product sizes were analyzed in comparison to migration of markers in 100 bp and 1 kb ladders (New England Biolabs).
-
bioRxiv - Neuroscience 2021Quote: ... and the product was inserted into a lentiviral expression vector (pSIH-H1) containing CMV promotor using NEBbuilder HIFI kit (NEW ENGLAND Biolabs). To co-express GFP-actin and mCherry-KDEL ...
-
bioRxiv - Immunology 2022Quote: ... then the PCR product was digested and ligated into SalI + XhoI digested pMSCV-neo vector with Quick Ligase (NEB, M2200L).
-
bioRxiv - Immunology 2022Quote: All CER constructs were generated by standard PCR cloning techniques and the PCR products were assembled using the NEBuilder HiFi DNA Assembly (New England Biolabs). CER21 was generated by linking the extracellular and transmembrane domain of human TIM-4 (GenBank™ accession number AAH08988.1 ...
-
bioRxiv - Cancer Biology 2022Quote: ... The PCR product was subcloned into the expression vector pFN31K-Nluc) using the XhoI and EcoRI restriction endonucleases (NEB, USA) and In-Fusion HD Enzyme (Takara ...
-
bioRxiv - Genomics 2022Quote: ... After 25 cycles of amplification the product was purified with the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030), and eluted with 20 μl of ddH2O ...
-
bioRxiv - Molecular Biology 2022Quote: ... 40 μL of each digest product were then ran on a 3% agarose gel and the appropriate band was gel extracted using a kit (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... 40 μL of digest product was then ran on a 3% agarose gel and the appropriate band was gel extracted using a kit (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... Ligation Test 1 was processed directly to index PCR while product in Ligation Test 2 was digested by 12.5 units of RNase H (NEB, Cat.No.M0297) at 37°C for 20min before index PCR.