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Citations for New England Biolabs :
2551 - 2600 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was amplified in two stages of Polymerase Chain Reaction (PCR) using Q5 DNA polymerase (NEB). For the first PCR reaction ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PCR reactions were cleaned up using NEBNext sample purification beads (New England Biolabs GmbH, Frankfurt, Germany). Concentration of the libraries was determined by Qubit using the Qubit dsDNA HS Assay Kit (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... Illumina TruSeq adapters were ligated to DNA fragments in a reaction containing 2X Quick ligation buffer (NEB), 0.25 μM of adapter and 2 μL of Quick ligase (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... Deproteinized reactions (63 μl) were incubated for 45 min at 37°C in 1X ThermoPol buffer (NEB) with 15 units E ...
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bioRxiv - Bioengineering 2024Quote: ... The reaction mixture for polymerization contained 25 units/ml Hot-start Taq DNA polymerase (New England Biolabs), Taq standard reaction buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Digested vectors to be used in ligation reactions were treated with recombinant shrimp alkaline phosphatase (NEB, M0371). PCR fragments and digested vectors were purified using GeneJET PCR Cleanup Kit (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... a 10 uL reaction is performed using 2.5 U of α2-3,6,8 neuraminidase (NEB; Cat. No. P0720L) and 1 U of α2-3,6,8,9 neuraminidase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... Each PCR reaction (25 µl) included 12.5 µl NEBNext Ultra II Q5 Master Mix (New England Biolabs), 30 ng genomic DNA ...
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bioRxiv - Microbiology 2024Quote: 50 µl PCR reactions were performed using Q5 Hot start High-Fidelity DNA polymerase (New England Biolabs). The resulting PCR products were digested with DpnI ...
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bioRxiv - Microbiology 2024Quote: Ubiquitination reactions containing recombinant GST-PKN1 were immunoprecipitated using protein G-conjugated magnetic beads (New England Biolabs). Beads were prepared by washing three times with IP wash buffer (PBS + 0.1% Tween-20) ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA was amplified by PCR in 25 μL reactions using Q5® High-Fidelity DNA Polymerase (NEB). All PCR reactions were set up according to the manufacturer’s instructions ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... PCR reactions to amplify sgRNA and CaCas9 were carried out using Phusion High-Fidelity DNA polymerase (NEB), and Q5 polymerase (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 500 ng of cDNA was used per reaction (Luna Universal qPCR Master Mix New England Biolabs: M3003X ...
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bioRxiv - Microbiology 2024Quote: ... DNA was amplified from cDNA using 10µl of 5x Q5 reaction buffer and 2.5µl Q5 enzyme (NEB), 0.5µl 100mM dNTPs ...
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bioRxiv - Microbiology 2024Quote: ... PCR reactions were carried out using 2x Q5 PCR master mix (New England Biolabs, Ipswich, MA, USA) and 1 µL of template (∼10 ng genomic DNA ...
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bioRxiv - Plant Biology 2024Quote: ... PCR reactions were performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs Inc., Ipswich, MA, USA) according to the manufacturer’s manual at an annealing temperature of 68 °C for 15 s followed by elongation at 72 °C for 30 s and PCR products separated by gel electrophoresis.
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bioRxiv - Bioengineering 2024Quote: ... ddPCR reactions were incubated at room temp for 1 hour with 1E5 units of SalI (NEB R3138L) to facilitate digestion of linear cAAV insertion products prior to droplet generation ...
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bioRxiv - Genomics 2024Quote: ... IVT used an in vitro transcription-translation reaction (PURExpress In Vitro Protein Synthesis Kit, NEB, Cat# E6800L) to express T7-driven ...
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bioRxiv - Genetics 2024Quote: ... PCR reactions were carried out using the Q5 High-Fidelity DNA polymerase kit (New England Biolabs (NEB), USA ...
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bioRxiv - Genetics 2024Quote: ... PCR reactions were carried out using the Q5 High-Fidelity DNA polymerase kit (New England Biolabs (NEB), USA ...
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bioRxiv - Genomics 2024Quote: ... and a multiplex PCR reaction using Q5 High Fidelity Hot-Start DNA Polymerase (New England Biolabs, UK) and the Peru RABV specific multiplex primer set ...
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bioRxiv - Genomics 2024Quote: ... we ligated an RT Adapter (RTA) to the samples using NEBNext® Quick Ligation Reaction Buffer (NEB), T4 DNA Ligase (2M U/ml ...
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bioRxiv - Bioengineering 2024Quote: ... Polymerase chain reaction (PCR) was conducted using Q5® High-Fidelity DNA Polymerase (New England BioLabs, USA). PCR products were incubated with DpnI (New England BioLabs ...
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bioRxiv - Synthetic Biology 2024Quote: ... and assembled together in one reaction by the Bsal restriction enzymes based Golden Gate Assembly (NEB, E1601). The inserts possess BsaI restriction sites at both ends in the proper orientation ...
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bioRxiv - Synthetic Biology 2024Quote: ... qPCR reactions were performed on 1 µL cDNA (diluted 1:8 in water) with Q5 polymerase (NEB), SYBR Green (Thermo Fisher) ...
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bioRxiv - Synthetic Biology 2024Quote: ... A test qPCR reaction was performed using 25 μL Q5 Ultra II High-Fidelity Polymerase (NEB #M0544L), 0.5 μL library pool ...
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bioRxiv - Synthetic Biology 2024Quote: ... The purified plasmid backbone and insert was ligated by T4 ligase reaction following the standard protocol (NEB). The ligated products were transformed into the dSL2 donor cell and selected on the appropriate LB+antibiotic plates.
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bioRxiv - Synthetic Biology 2024Quote: GFP fluorescence during cell free expression was performed using 25 µl reactions with PurExpress (New England BioLabs) in 384-well black microplates ...
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bioRxiv - Cell Biology 2024Quote: ... and qRT-PCR was performed in duplicate reactions using the LunaScript® RT SuperMix Kit (NEB, E3010). Expression values were normalized to HRPT-1 and GAPDH levels for GFP+ and tdTomato+ cells ...
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bioRxiv - Genetics 2024Quote: ... RT-qPCR reactions were carried out using the Luna Universal One-Step RT-qPCR Kit (NEB, #E3005L). Quantification was performed using a SYBR Green-based method on an Eppendorf Realplex Mastercycler (software version 2.2.0.84) ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 ng of mRNA was decapped using either tobacco acid pyrophosphate (250 U; Epicenter) or RppH (NEB) in buffer provided by the supplier and then dephosphorylated by Antarctic phosphatase (NEB) ...
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Mechanism of the ANT-mediated transport of fatty acid anions across the inner mitochondrial membranebioRxiv - Biophysics 2022Quote: ... and aspartic acid 134 to serine using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Austria). The sequences were verified by both DNA and amino acid sequencing ...
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bioRxiv - Synthetic Biology 2024Quote: ... nucleic acids were digested by addition of 10 µL of DNaseI (2000 units/mL, New England Biolabs) and CaCl2 to a final concentration of 10 mM ...
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bioRxiv - Biochemistry 2023Quote: ... or amino acid substitutions was carried out using the Q5 DNA polymerase and KLD enzyme mix (NEB). Plasmid DNA was extracted and purified from 5 ml or 250 ml bacterial culture using Miniprep (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... Complementary deoxyribonucleic acid (cDNA) was synthesized using the ProtoScript First-Strand cDNA Synthesis kit (New England Biolabs). Real-time PCR assays were performed using a CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... Truncated EndoS1 lacking the first 9 amino acids was created by Q5 mutagenesis (New England Biolabs, NEB) by amplifying the full-length construct using primer pairs P154/P155 ...
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bioRxiv - Biochemistry 2024Quote: ... Truncated EndoS1 lacking the first 9 amino acids was created by Q5 mutagenesis (New England Biolabs, NEB) by amplifying the full-length construct using primer pairs P154/P155 ...
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bioRxiv - Cancer Biology 2021Quote: ... The PCR products were resolved by agarose gel electrophoresis and purified using the Monarch Gel Extraction kit (New England Biolabs). Purified PCR products were Sanger sequenced by Genewiz using the BPNT1_F_Seq primer ...
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bioRxiv - Cell Biology 2020Quote: ... The extended DNA products were purified using Expin PCR SV mini (GeneAll) and transcribed by T7 RNA polymerase (New England Biolabs). After incubation at 37 °C for 16 h ...
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bioRxiv - Genetics 2021Quote: ... The digest products were then ethanol precipitated and self-ligated at room-temperature overnight using T4 DNA Ligase (New England Biolabs). The resulting PCR products were restriction-digested with EagI (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were individually treated for 16 h at 37 °C with 20 units of DpnI enzyme (New England Biolabs). Following gel extraction using Wizard SV Gel and PCR CleanUp System (Promega) ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were gel purified and transcribed in vitro using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). Cas9 mRNA was synthesized from the pX330 plasmid using the mMESSAGE mMACHINE® T7 Ultra (Thermofisher ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products of ITS and EST104 were digested with DdeI and RsaI (New England Biolabs Japan, Inc., Tokyo, Japan), respectively ...
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bioRxiv - Developmental Biology 2020Quote: ... and a 481-bp pph-5 3’UTR and assembled the PCR products with amplified pUC57 backbone via HiFi assembly (New England Biolabs) to generate the vector pph-5p::pph-5(cDNA)::pph-5 3’UTR (TU#2257) ...
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bioRxiv - Genomics 2020Quote: ... Beads containing the ssDNA extension products were suspended in 10 μl of polyadenylation master mix containing 2 units of terminal transferase TdT (New England Biolabs), 1x TdT buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Mpe1P215G and Mpe1W257A/Y260A variants were similarly generated with PCR products carrying the respective mutations and Gibson assembly into a BamHI (NEB) digested pACEBAC_TEV_SII vector ...
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Direct analysis of ribosome targeting illuminates thousand-fold regulation of translation initiationbioRxiv - Molecular Biology 2020Quote: ... Gel-purified cDNA products were ligated to the adapter oWG920 (Supplementary Table 6) using T4 RNA ligase 1 (NEB M0437M). cDNA cleanup was performed using 10 µl MyOne Silane beads (Thermo Scientific 37002D ...
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bioRxiv - Molecular Biology 2020Quote: ... the sgRNA products synthesized by VSW-3 RNAP and T7 RNAP were purified with Monarch RNA Cleanup kit (New England Biolabs) and then further purified by high performance liquid chromatography (HPLC ...
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bioRxiv - Genomics 2022Quote: ... The purified ligation product was electroporated into 100 μL of 10-beta E.coli (NEB, C3020K, 2kV, 200 ohm, 25 μF) in order to achieve a transformation efficiency equal to 200-times the number of unique oligos combinations (target CFU ...
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bioRxiv - Genomics 2022Quote: Library preparation was started with DNA repair and end-prep of 50 fmols of lrPCR mtDNA amplification product using NEBNext FFPE DNA Repair Buffer (NEB), NEBNext FFPE DNA Repair Mix (NEB) ...