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Citations for New England Biolabs :
2551 - 2600 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... was amplified with KAPA polymerase using primers #545 and #546 (Table S5) and purified with Monarch DNA Cleanup Columns (NEB). Both intermediate construct and amplified gBlock were digested with BbsI (NEB ...
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bioRxiv - Genetics 2019Quote: ... encoding a scaffold and the mouse U6 promoter was amplified with KAPA polymerase using primers #545/#546 (Table S5) and purified with Monarch DNA Cleanup Columns (NEB). Both intermediate library and amplified gBlock were digested with BbsI (NEB ...
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bioRxiv - Genetics 2020Quote: ... An 18-mer poly-N barcode was created by annealing primers (Table S1) and extended to make fully double stranded with Klenow polymerase (NEB). The barcode was then PCR purified (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... using 35 cycles with an annealing temperature of 60 °C (see table 2 for list of primers) and cloned by Gibson Assembly Cloning Kit (EE5510S, NEB). All primers were designed using SnapGene (GSL Biotech LLC ...
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bioRxiv - Microbiology 2022Quote: ... Backbone was amplified with primer pair 3/4 and fragments were assembled with NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs).
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bioRxiv - Cell Biology 2022Quote: ... treatment on the total RNA was performed and cDNAs were generated using the M-MuLV Reverse Transcriptase and random primers (NEB), as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Radioactive probe was prepared using 40 pmol of primer 59 and 5’-labelled with 10 U of T4 Polynucleotide Kinase (New England Biolabs) and [γ32P]ATP (150 μCi) ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... DNA fragments were amplified with 15-20 bp of homology regions in primers using Phusion R High Fidelity DNA polymerase (New England Biolabs; NEB) and homology based assemblies were performed using NEBuilder® HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cRFXa CDS was amplified from cDNA using primers MC252 and MC253 and Q5 DNA polymerase (NEB Cat. No. M0491L), with 2 µl of cDNA template in a 50 µl PCR reaction and 35x cycles ...
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bioRxiv - Plant Biology 2022Quote: ... Each fragment was PCR amplified using the primers listed in Supplementary Table 1 and was cloned into the pCAMBIA1300 using the T4 DNA ligase (NEB). The construct was transformed into the atalkbh9b-1 mutant and the transgenic lines were identified for homozygosity at T3 generation.
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bioRxiv - Synthetic Biology 2022Quote: The detection in the specific primer amplification experiments was performed with a lower concentration of LbCas12a (1μM, NEB, Cat. # M0653S). Interestingly ...
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bioRxiv - Microbiology 2022Quote: ... and an equimolar mix of primers P5-IR2a-d primer (10 µM) in a reaction with 50 ng of adaptor ligated template and Phusion DNA polymerase (NEB) in a thermocycler with the following program 98°C 3 min ...
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bioRxiv - Microbiology 2022Quote: ... The library of circular DNA fragments was then used as a PCR template with forward and reverse primers specific to the transposable element and amplified using Phusion High-Fidelity DNA Polymerase (New England Biolabs). PCR products were separated by electrophoresis on a 1% agarose gel and purified using the Zymoclean Gel DNA recovery Kit (Zymo Research ...
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bioRxiv - Microbiology 2022Quote: ... Vector pET16b and gene mksB (cg3103) were amplified using primer pair MG093/MG094 and MG095/MG096 respectively and cloned using the Gibson Assembly Mastermix (NEB), resulting in plasmid pMG004 ...
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bioRxiv - Systems Biology 2022Quote: ... The sgRNA oligo pool with restriction sites and flanking primer sequences was synthesized by Twist Bioscience and was amplified using Q5 polymerase (NEB). The PCR reaction was carried out as following ...
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bioRxiv - Plant Biology 2022Quote: ... For quantitative PCR 90 ng of template DNA was used in 10 μl qPCR reactions with 0.25 μM primers and Luna qPCR Master Mix (NEB, USA). qPCR was performed in a LightCycler 480 (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... PCR was performed with primers 515F–806R targeting the V4 region of the 16S rRNA using Phusion High-Fidelity DNA Polymerase (NEB). The PCR product concentration was measured with Quant-iT PicoGreen dsDNA Assay Kits (Invitrogen ...
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bioRxiv - Synthetic Biology 2022Quote: ... from Broccoli single-stranded DNA sequence (100 ng in 20 μL reaction mixture) amplified using Broccoli forward and reverse primers (0.5 μM) and Phusion® DNA polymerase (NEB). An initial denaturation at 98 °C for 30 sec was followed by denaturation at 98 °C for 10 sec ...
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bioRxiv - Molecular Biology 2022Quote: ... Molecular cloning of the genes encoding BacA-related and YejABEF-related transporters into the pSRK vector [23] was performed either by conventional restriction enzyme digestion and ligation protocol (restriction sites are specified for each gene in the corresponding primer names) or by Gibson Assembly protocol (NEB). For Gibson Assembly pSRK plasmid was PCR-amplified with primers pSRK_GA_F and pSRK_GA_R and treated with DpnI restriction endonuclease (ThermoFisher).
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bioRxiv - Synthetic Biology 2022Quote: ... or PCR amplified using primers that anneal to the 5’ or 3’ ends of each chunk with Phusion polymerase (New England Biolabs). Plasmid digested or PCR amplified chunks were excised from agarose gels or column purified ...
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bioRxiv - Plant Biology 2022Quote: ... by PCR based site-directed mutagenesis (SDM) using non-overlapping primers listed in Supplementary Table S1 and Phusion High-Fidelity DNA Polymerase (New England Biolabs). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... Tiled 1200 bp amplicons were generated using midnight primers (IDT) with Q5 Hot Start High-Fidelity 2X Master Mix (NEB) for 32 cycles (LA-GSU1 to LA-GSU19 ...
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bioRxiv - Microbiology 2022Quote: ... the pulL gene was PCR-amplified from plasmid pCHAP8258 as template using primers PulL Kpn 5 and PulL Eco 3 with the high-fidelity Q5 DNA polymerase (New England Biolabs). The PCR products were purified on a Qiaquick spin column ...
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bioRxiv - Microbiology 2024Quote: ... the coding regions were amplified with primers listed in Supplementary file Table S1B from chromosomal DNA using Phusion high-fidelity DNA polymerase (NEB) and subjected to restriction enzyme digestion ...
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bioRxiv - Synthetic Biology 2024Quote: Constructs were designed in silico in Benchling and primers for the Gibson reaction were obtained from the NEBuilder website (New England Biolabs). Primers were purchased from Sigma-Aldrich in cartridge purified quality and used in PCR reactions with the Q5 high fidelity (NEB ...
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bioRxiv - Pathology 2024Quote: ... Unincorporated primers were removed by Exonuclease digestion for 30 min at 37°C followed 15min inactivation at 80C (New England BioLabs). Samples were diluted 5-fold with DNA suspension buffer (Teknova) ...
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bioRxiv - Cancer Biology 2024Quote: ... by PCR amplification with the indicated primers (Key Resources table) followed by a digestion with Bam HI and Xba I enzymes (New England Biolabs) for 37°C 2 h ...
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bioRxiv - Cell Biology 2023Quote: ... DNA libraries were produced using NEBNext Ultra II DNA Library Preparation kit and NEBNext Unique Dual Index Primers (New England Biolabs). Libraries were quality checked using Agilent D1000 TapeStation kit and Qubit Flourometer and then sequenced on the Illumina NovaSeq X (Admera Health ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.2 µM of each primer were amplified with 0.5 units of One Taq DNA polymerase (Biolabs, Ipswich, MA, USA). Cycling conditions were established with an initial denaturation step at 95°C/2 min followed by 40 cycles at 95 °C/45 s ...
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bioRxiv - Molecular Biology 2024Quote: ... The CHD4-GFP mutant was cloned with selective primers (fwd, 5’-GGATGCTACAGGTGGAACCCTGCACCCCTA-3’; rev, 5’-GCCCAGGCCCGACGCCAT-3’) and a Q5 site-directed mutagenesis kit (NEB) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: 100 ng polyA+ RNA was annealed with 2 μl of 10 μM Oligo(dT)30VN primer (5’-TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’) and 2 μl of 10 mM dNTP mix (NEB) in 12 μl solution ...
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bioRxiv - Microbiology 2024Quote: ... The SV40 NLS was added to the C-terminus of CypA via annealing partially complimentary primers encoding the SV40 NLS (Table S1) at 20 μM with NEB buffer 2 (New England Biolabs) at 95° C for 4 min and 70° C for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Barcoded libraries were made with NEBNext Ultra II DNA Library Prep Kit for Illumina) using NEBNext Multiplex Oligos Dual Index Primers for Illumina (New England BioLabs) and sequenced on NextSeq2K (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... were generated from a pENTR cyfip2-EGFP plasmid [32] using custom primers and the Q5 Site Directed Mutagenesis Kit (NEB) to induce the desired C179R (ΔRac1 ...
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bioRxiv - Microbiology 2024Quote: ... Four PCR fragments of around 2,700 nucleotides long were generated from cDNA using primer pairs (Suppl. Table S2) with NEB Q5 Hot-Start high-fidelity 2× Master Mix (New England Biolabs). Fragments were gel-purified with MinElute gel extraction Kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... The radioactive probe was prepared from 40 pmoles of D072 primer (Table 1) and labeled at the 5’ end with 10 units of T4 polynucleotide kinase (New England Biolabs) and [γ-32P]-ATP (150 μCi) ...
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bioRxiv - Microbiology 2024Quote: ... On-bead PCR indexing-amplification was performed using custom-ordered indexing primers (IDT) matching the Illumina Nextera Index Kit sequences and 2x Phusion Master Mix (NEB). PCR reactions were amplified for 9-11 cycles ...
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bioRxiv - Cell Biology 2024Quote: ... and 5’-ACG CGT ACT AGT CGA TCG CTT GTA CAG CTC GTC CAT G-3’ (reverse primer) and using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 5µM of NEBNext Unique Dual Index Primers and 25 µL NEBNext Q5U Master Mix (New England Biolabs, Ipswich, MA, USA) were added to the DNA and PCR amplified ...
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bioRxiv - Genetics 2024Quote: QF2-T2A-LexA-GAD ORF PCR-amplified with FRT-QF2-fwd + FRT-lexA-GAD-rev primer pair – used Hot-Start Q5 polymerase with GC enhancer (NEB);
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bioRxiv - Plant Biology 2024Quote: ... A 517bp product was amplified using the primers specified in Table S4 and S5 and digested with BsaI-HFv2 (NEB) according to the manufacturer’s guidelines before gel electrophoresis of the resulting product on 3% agarose gel ...
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bioRxiv - Microbiology 2023Quote: ... the entire region of pET15b-His-sdeA except for the region encoding 1-199 aa of SdeA was amplified with primers 2649/2712 and then the fragment was self-ligated with a Gibson assembly kit (New England Biolabs). For construction of pmGFP-sdeAΔDUB ...
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bioRxiv - Cell Biology 2024Quote: ... with primers BA3187/BA3188 and cloned into RSFDuet-1 using BamHI/EcoRI sites with the NEBuilder HiFi DNA Assembly kit (NEB) (Table S1) ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA resistant mCherry-ALG-2 was created by mutagenesis (primers sequences 5’-ATTTCGATGTTTGACCGTGAGAAC-3’, 5’-AATGGACCTGACAGTCACTGGATTAAA-3’) using a Q5 Site-Directed Mutagenesis Kit (E0554S, NEB). The plasmid encoding IST1-GFP is as described (63 ...
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bioRxiv - Genetics 2024Quote: ... Genome PCR products which were confirmed as a single band in AGE were subjected to direct Sanger sequencing after decomposing remaining nucleotides and primers by the treatment with 2U Exonuclease I (NEB) and 0.1U Shrimp Alkaline Phosphatase (NEB).
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bioRxiv - Microbiology 2024Quote: ... The CUP1 promoter sequence was amplified from the iBFG-Y2H vectors (primers oHM487/488) and cloned into N2H N1 and N2 vectors digested with SpeI/SacI using NEBuilder assembly (NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... according to manufacturer’s instructions using 100 nM RNA-specific reverse transcription primer followed by RNase H digest with 5 µL RNase H (NEB) at 37 °C for 20 min ...
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bioRxiv - Biochemistry 2024Quote: ... were PCR amplified with indicated primers (see Table S1) and inserted into backbone plasmids via restriction digest and ligation (all enzymes from NEB) or Gateway cloning (Thermo) ...
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bioRxiv - Cell Biology 2024Quote: ... Site directed mutagenesis was used to create an additional Lv165 BMI1 overexpression vectors with a P2A sequence instead of the IRES by designing primers using the New England Biolabs (NEB)-base changer website (https://nebasechanger.neb.com ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 ng of the intermediate plasmid products were incubated with 10 uM random hexamer primers and 1X phi29 DNA polymerase buffer (NEB) at 95°C for 3 minutes and then cooled to room temperature ...