Labshake search
Citations for New England Biolabs :
2351 - 2400 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... Proteinase-K (PK) reaction mixes were prepared by diluting Proteinase K (20 mg/ml stock, NEB) to a final concentration of 2 mg/ml in the PK buffer and incubated at 37°C for 20 minutes to deactivate any RNAse activity ...
-
bioRxiv - Molecular Biology 2021Quote: ... Customized PURExpress reconstituted systems lacking all amino acids and release factors (Δaa, ΔRF123) (New England Biolabs) (Fig ...
-
bioRxiv - Microbiology 2022Quote: ... aliquots of 200 ng nucleic acid were treated with 2 U DNase I (New England Biolabs), 10 U S1 nuclease (Thermo Scientific) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Genomic deoxyribonucleic acid (gDNA) was extracted using Monarch Genomic DNA Purification Kit (New England BioLabs, T3010S) following procedures for Tissue gDNA isolation ...
-
bioRxiv - Genomics 2023Quote: ... 10 μL of extracted nucleic acid was treated with DNase I (New England Biolabs, Ipswich, MA), followed by a clean up step using a ratio of 1.8:1 beads to sample ...
-
bioRxiv - Molecular Biology 2024Quote: ... To digest sialic acid: 2 μL of α2-3,6,8 Neuraminidase (50U/mL, New England Biolabs, NEB) with GlycoBuffer 1 (NEB ...
-
bioRxiv - Molecular Biology 2024Quote: ... To digest sialic acid: 2 μL of α2-3,6,8 Neuraminidase (50U/mL, New England Biolabs, NEB) with GlycoBuffer 1 (NEB ...
-
bioRxiv - Biochemistry 2024Quote: ... Single amino acid substitutions were introduced using NEB’s site-directed mutagenesis kit (New England Biolabs, E0552S). Primers used for cloning and site-directed mutagenesis are listed in Table S2 ...
-
bioRxiv - Cell Biology 2020Quote: ... PCR amplified products were generated and two-or three-piece Gibson assembly was performed per manufacture’s instruction (New England Biolabs)
-
bioRxiv - Genetics 2021Quote: ... 20 μl of this product was used for PCR amplification using Q5 Hot Start High-Fidelity polymerase (NEB #M0494S) and a unique combination of the dual barcoded primers P5 and P7 Nextera XT Index kit (Illumina #15055293 ...
-
bioRxiv - Developmental Biology 2021Quote: ... To remove the adaptor sequences from the resulting PCR products up to 2.5 μg of product was transferred to a new 1.5 ml tube and was digested with AlwI restriction enzyme (New England Biolabs) at 37 °C for 16 hours ...
-
bioRxiv - Molecular Biology 2021Quote: sgRNA products synthesized by T7 RNAP WT and S43Y mutant were purified with RNA purification kits (New England Biolabs) before analyzed by high performance liquid chromatography (HPLC ...
-
bioRxiv - Molecular Biology 2021Quote: ... The PCR product was purified using Monarch PCR and DNA Cleanup kit (New England Biolabs, Inc., Ipswich, MA, USA) and DNA concentration was quantified using NanoDrop spectrophotometer (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2020Quote: ... PCR products were cleaned using one unit each of Exonuclease I and Antarctic Phosphatase (New England Biolabs, Massachusetts, USA), with sequencing reactions performed using an ABI3730 Genetic Analyzer (Applied Biosystems ...
-
bioRxiv - Biochemistry 2021Quote: ... and then digested PCR product and CIP-treated vector backbone were ligated with T4 DNA ligase (New England Biolabs). Ligation reactions were transformed into chemically competent E ...
-
Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 2 μl of the circularized product was then used for PCR amplification using Phusion High-Fidelity DNA Polymerase (NEB) for a maximum of 16 cycles ...
-
bioRxiv - Biochemistry 2020Quote: ... and then the product was cloned into the pCINeo-IRES-GFP vector using NheI and XhoI (New England Biolabs). To obtain truncated CNNM2 plasmids ...
-
bioRxiv - Systems Biology 2021Quote: ... all DNA samples were prepared for Illumina sequencing using the NEBNext Ultra DNA Library Prep Kit (NEB product #E7370), with either single index or dual index primers also obtained from NEB ...
-
bioRxiv - Microbiology 2020Quote: ... PCR products were then incubated at 65°C for 30 minutes in the presence of BsmI restriction enzyme (NEB). The resulting DNA fragments were separated by 1.5% agarose gel electrophoresis ...
-
bioRxiv - Immunology 2021Quote: ... Restriction digest of EtMIC3 PCR product was performed using Bam HI and Eco RI restriction enzymes (New England Biolabs) to extract antigen coding sequence for cloning into pYD1 plasmid vector ...
-
bioRxiv - Microbiology 2020Quote: ... all PCR products were purified using the Monarch® PCR and DNA Clean-up Kit (New England Biolabs, USA) following the manufacturer’s instructions and eluted in molecular grade water.
-
bioRxiv - Plant Biology 2020Quote: ... The 839-bp PCR product was digested with SapI and XhoI and ligated into SapI–XhoI-digested pTYB11 (NEB), generating pMS451 ...
-
bioRxiv - Microbiology 2021Quote: ... A 470 bp PCR product surrounding the UL23 cut site was amplified using Phusion high-fidelity polymerase (NEB, USA) and column purified (Macherey-Nagel ...
-
bioRxiv - Cancer Biology 2022Quote: ... The PCR product was then gel purified with NEB Monarch® DNA Gel Extraction Kit (NEB, Cat No. T1020). Alongside pLentiCRISPRv2 (Addgene ...
-
bioRxiv - Microbiology 2022Quote: ... The digested product was then ligated with a NdeI and HindIII cut pCT94 using Gibson assembly (NEB, Ipswich, MA). The assembly product (CT94-lpdA ...
-
bioRxiv - Synthetic Biology 2022Quote: ... in which 20-50 ng of uncut acceptor plasmid and a 2:1 molar ratio of donor plasmid or PCR products were mixed together with 1.5 μl DNA T4 ligase buffer (#B0202S; NEB), 0.0015 mg BSA (B9000S ...
-
bioRxiv - Molecular Biology 2022Quote: ... Failed ligation products and gDNA fragments were removed by nuclease treatment using 1.2U/ul of Exonuclease III (New England BioLabs) and an Enzyme Clean Up Kit (Pacific Biosciences ...
-
bioRxiv - Microbiology 2022Quote: ... The gel-purified PCR products were assembled together using NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... The PCR products (521 bp) were then treated with the restriction enzyme Bsp1286I (New England Biolabs, Ipswich, MA, USA), and the F54L mutant DNA was digested to 260 bp and 261 bp fragments ...
-
bioRxiv - Microbiology 2021Quote: ... The PCR products were assembled by Gibson assembly using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, USA).
-
bioRxiv - Microbiology 2022Quote: ... Digested pAd5-Blue and IE1 PCR products were then ligated using T4 DNA ligase (New England Biolabs, Ipswich, MA) according to manufacturer’s instructions ...
-
bioRxiv - Genetics 2022Quote: ... The digested PCR products and the pBSMVγ plasmid were ligated using T4 ligase (New England BioLabs, catalog number M0202). This plasmid was named pBSMVγQT1 (Figure 1b) ...
-
bioRxiv - Microbiology 2022Quote: ... and the fucI PCR product was cloned into the plasmid using the NEBuilder HiFi DNA Assembly Kit (NEB; E5520S), followed by transformation into the E ...
-
bioRxiv - Bioengineering 2020Quote: ... 200 ng of ligation product from the first stage were digested by Not I-HF (New England Biolabs, R3189S) and heat denatured ...
-
bioRxiv - Microbiology 2020Quote: ... The resulting PCR product was cloned into pDC_A1G3 pre-digested with Sal I using Gibson Assembly master mix (NEB) according to the manufacturer’s instructions giving rise to plasmid pDC_A1G2/3.
-
bioRxiv - Pathology 2021Quote: ... Fragmented PCR products were repaired and dA-tailed using the NEBNext End Repair/dA Tailing kit (New England Biolabs) according to the manufacturer’s instructions and subsequently barcoded with Illumina-compatible ...
-
bioRxiv - Molecular Biology 2021Quote: ... F1 dumpy animals were isolated and genotyped by PCR with let-7_SEQ_F5/R5 primers followed by an analysis of the PCR product using restriction digestion using EcoRV (NEB, Cat:N3195) and Sanger sequencing as described in (Duan et al. ...
-
bioRxiv - Genetics 2020Quote: ... The Rad21l1 PCR product was then cloned into pET28b using NEBuilder HiFi DNA Assembly Master Mix (NEB, Catalog #: E5520S). BL21 (DE3 ...
-
bioRxiv - Genetics 2021Quote: ... Five µl of the hybridization product was combined with 1 µl of 20 µM Cas9 protein (New England Biolabs) at room temperaturefor 5 minutes ...
-
bioRxiv - Molecular Biology 2021Quote: ... The ligated product was transformed into Escherichia coli (E. Coli) Stbl competent cells (New England Biolabs, Ipswich, MA, USA). The clones were picked and screened using colony PCR (Supplementary Table S1) ...
-
bioRxiv - Cell Biology 2021Quote: ... Purified NLS-mNeptune2-T2A and ccz1 backbone PCR products were assembled together using NEBuilder HiFi Assembly cloning kit (NEB).
-
bioRxiv - Molecular Biology 2021Quote: PCR products were subject to in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (E2040S, NEB), which relies on the T7 RNA polymerase to initiate transcription from a T7 promoter sequence (present in our fragments) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 5 µL of the assembly product was used to transform 50 µL of DH5α chemically competent cells (NEB #C2987I) according to the manufacturer’s high-efficiency transformation protocol ...
-
bioRxiv - Developmental Biology 2022Quote: ... PCR products were separated by electrophoresis on 1.5% agarose gels stained with Green Gene Safe DNA Dye (Southern BioLabs). PCR products were purified using Zymo’s DNA Clean and Concentrator kit and sequenced with their respective primer sets to confirm correct insertion of the fluorescent tag.
-
bioRxiv - Microbiology 2023Quote: ... Gibson assembly was performed using the generated PCR products and linearized pACYC184 according to the manufacturer’s protocol with HiFi DNA Assembly Master Mix (New England Biolabs). The pdxAoperon amplicon and pACYC184 was ligated after digestion using T4 DNA ligase according to the manufacturer’s protocol (New England Biolabs) ...
-
bioRxiv - Bioengineering 2023Quote: ... Five µl of the KLD product was used directly to transform chemically-competent Escherichia coli DH5ɑ cells (NEB C2988) according to the manufacturer’s protocol and plated on lysogeny broth (LB ...
-
bioRxiv - Genetics 2023Quote: ... The isolated ePCR products were inserted into a BsmBI-digested lentiV2-mU6-evopreQ1 vector via Gibson assembly (NEB E2621L). The assembled products were electroporated into Endura electrocompetent cells (Biosearch Technologies 60242) ...
-
bioRxiv - Genomics 2023Quote: ... Then equal amounts of purified linear A and B PCR products were mixed with the Taq DNA ligase (NEB) and its buffer ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and successful PCR products were cleaned up using Monarch® PCR and DNA Clean-Up Kit (New England Biolabs) as per kit instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... The purified IP products were prepared into a library using NEBNext Ultra II DNA library prep kit (NEB E7103) using manufacturer’s protocol ...