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Citations for New England Biolabs :
2451 - 2500 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The gRNA cassette was amplified using the NEBNext® High-Fidelity 2X PCR Master Mix (NEB) and primers including Illumina adaptors and 8nt barcodes to allow Next Generation Sequencing analysis (Supplementary Table 2) ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich MA, USA). For ITS we used the following conditions ...
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bioRxiv - Microbiology 2023Quote: ... Amplification of the products was performed using Q5 high-fidelity DNA polymerase (New England Biolabs, USA) according to the guidelines of the producer with 2 μM of 5’-GTT TCC CAG TCA CGA TA-3’ ...
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bioRxiv - Molecular Biology 2023Quote: ... downstream of a T7 promoter using overlap-extension PCR with Q5 High-Fidelity DNA Polymerase (NEB) and InFusion cloning (Takara Bio) ...
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bioRxiv - Developmental Biology 2023Quote: ... were amplified in NEB® Stable Competent Escherichia coli (High Efficiency) cells (New England BioLabs, #C3040), purified using NucleoSpin Plasmid kit (MACHENREY-NAGEL ...
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bioRxiv - Immunology 2023Quote: ... All cloning PCR amplifications were performed using the Q5 High-Fidelity DNA Polymerase (New England Biolabs) according to standard procedures ...
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bioRxiv - Bioengineering 2024Quote: ... PCR replication of Geneblocks was conducted using Q5® Hot Start High-Fidelity Master Mix (NEB; as per the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... we amplified the oligos for each tile (Q5 Hot Start High-Fidelity 2X Master Mix, NEB). In parallel ...
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bioRxiv - Genetics 2024Quote: ... and libraries were generated by PCR with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541). Prior to sequencing ...
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bioRxiv - Genomics 2024Quote: ... Libraries were amplified for 12 cycles using NEBNext® High-Fidelity 2X PCR Master Mix (NEB) in total volume of 25µl in the presence of 800nM of barcoded primers (400nM each ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3% dimethyl sulphoxide (DMSO) and 0.4 U of Taq-Phusion High-Fidelity (New England Biolabs, USA). PCR conditions were as follows ...
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bioRxiv - Genetics 2024Quote: ... and all PCR products were generated with Phusion® High-Fidelity DNA Polymerase (New England BioLabs) and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel).
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bioRxiv - Genomics 2024Quote: ... and Q5® Hot Start High-Fidelity 2X Master Mix (NEB, Cat No. M0494, Ipswich, MA), according to the recommended reaction conditions (Supplemental 8) ...
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bioRxiv - Genetics 2024Quote: ... and all PCR products were generated with Phusion® High-Fidelity DNA Polymerase (New England BioLabs) and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel) ...
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bioRxiv - Molecular Biology 2024Quote: ... hnRNPA1 effector domain was amplified from HEK293T cDNA using the Q5 High fidelity polymerase (#M0491S, NEB). Overhanging BsWI sites were added to the oligonucleotides for cloning into the dCasRx plasmid with Quick Ligation kit (M2200S ...
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bioRxiv - Molecular Biology 2024Quote: ... CD46 or PKM whole transcript cDNA was amplified using the Q5 High fidelity polymerase (#M0491S, NEB) and the oligonucleotides indicated in Supplementary Table 4 using the program ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplification of target sequences under 5 kb were produced using Q5 high fidelity polymerase (NEB # M0491S) under the recommended three-step amplification conditions ...
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bioRxiv - Molecular Biology 2024Quote: ... Inserts and fragments generated by PCR were synthesized using Q5 High Fidelity DNA Polymerase (NEB, M0491). All primers were synthesized by Integrated DNA Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... genomic sites of interest were amplified with the Q5® High-Fidelity 2X Master Mix (NEB) using conventional thermocycling conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... Inverse PCR was performed using 4C_ALKATI_EcoRI or 4C_ULK4int31_NlaIII (reading) and 4C_ALKATI_DpnII or 4C_ULK4int31_DpnII (non-reading) primers with 2x Phusion high-fidelity PCR mix (NEB). Each 4C sample was amplified in 4 PCR reactions ...
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bioRxiv - Microbiology 2024Quote: ... 16S ribosomal RNA (rRNA) was amplified with Phusion High-Fidelity polymerase (New England Biolabs, Ipswich, MA) and dual indexed primers (319F ...
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bioRxiv - Cancer Biology 2024Quote: ... purified with tRNA-coated streptavidin beads and amplified with 2x Phusion high-fidelity PCR mix (NEB). CAGE libraries were treated with Exonuclease I (NEB ...
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bioRxiv - Microbiology 2024Quote: ... PCR reactions were carried out using Phusion high-fidelity DNA polymerase (New England Biolabs, Ipswich, MA). Plasmids were digested using NEB high-fidelity restriction endonucleases in Cutsmart buffer and plasmid assembly reactions were performed with NEBuilder HiFi DNA assembly ...
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bioRxiv - Microbiology 2020Quote: ... Ligations were performed in 96 well-plates with 1U T4 Ligase (NEB) and incubated at room temperate overnight ...
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bioRxiv - Cell Biology 2023Quote: ... the plate libraries were pooled together and AmpureXP beads (New England BioLabs) were used to purify the cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were resolved on an 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane for detection with the following antibodies: anti-Glucocorticoid Receptor D6H2L (1:1000 dilution, Cell Signaling, New England BioLabs #12041), anti-PAX7c (1:500 dilution ...
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bioRxiv - Genomics 2020Quote: ... 2.5 μl of 10 μM NEBNext i5 and 2.5 μl of 10 μM NEBNext i7 primers (NEB) in a final volume of 50 μl ...
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bioRxiv - Cell Biology 2022Quote: ... annealed and phosphorylated sgRNA (1:10) and T4 Ligase reaction buffer (1:10, #B0202S, New England Biolabs) with 10 mM 10x Adenosine 5’-Triphosphate (#P0756S ...
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bioRxiv - Genetics 2020Quote: ... 10 mL of a 10% BSA solution was incubated with 2.5 μL of Proteinase K (NEB P8107S) for 30 minutes at room temperature (∼22°C) ...
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bioRxiv - Genomics 2024Quote: ... 25 µL 10x CutSmart buffer and 10 µl 10 U/µL MseI restriction enzyme (New England Biolabs) were added ...
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bioRxiv - Plant Biology 2020Quote: ... RGA::dRGA was amplified from genomic DNA of Col-0 using Q5 high fidelity DNA polymerase (NEB). Two overlapping fragments lacking 17 aminoacids covering the DELLA domain like described in (Feng et al ...
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bioRxiv - Synthetic Biology 2021Quote: ... Linear DNA templates from IDT were amplified by PCR using Q5 High fidelity DNA polymerase (NEB, #M0491S) and dNTPs (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... We quantified the cDNA with NanoDrop and performed PCRs with Q5 Hot Start High Fidelity polymerase (NEB) using equal amounts of obtained cDNA from each tissue sample to detect presence of TMEM16K (primer set 1 ...
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bioRxiv - Cell Biology 2020Quote: ... The fragments for the assembly were prepared by PCR using Q5 High Fidelity polymerase (New England Biolabs). The plasmids used along with details on their preparation are listed in Supplementary Table 2.
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bioRxiv - Molecular Biology 2021Quote: ... two independent amplicons were generated by PCR using Q5 Hot Start High-Fidelity 2X Master Mix (NEB). One amplicon for the targeted locus and one amplicon of the pegRNA locus (primers listed in Supplementary Table 1 ...
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bioRxiv - Microbiology 2021Quote: ... Chitinase genes and their endogenous promoters were amplified using Q5 High-Fidelity DNA Polymerase (New England Biolabs) with wild-type Salmonella IR715 as the template and the primer sets STM0233_Tn7Com_FW and STM0233_Tn7Com_RV or chiA_Tn7Com_FW and chiA_Tn7Com_RV ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We then performed PCR reactions at 12 cycles using Phusion High-Fidelity PCR Master Mix (NEB Biolabs) and primers that bind to common regions in the adaptors ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We then performed PCR reactions at 12 cycles using Phusion High-Fidelity PCR Master Mix (NEB Biolabs) and primers that bind to common regions in the adaptors ...
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bioRxiv - Developmental Biology 2020Quote: ... Final library for paired-end sequencing was prepared using NEBNext High-Fidelity 2X PCR Master Mix (NEB). PCR reaction ...
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bioRxiv - Developmental Biology 2020Quote: ... Final library for paired-end sequencing was prepared using NEBNext High-Fidelity 2X PCR Master Mix (NEB) and a nested PCR approach as described in Schwartzman et al ...
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bioRxiv - Neuroscience 2021Quote: ... Site directed mutagenesis was performed using overlapping PCR with Phusion® High-Fidelity DNA Polymerase (NEB M0530S) and primer sequences mentioned in Table 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Q5 High-Fidelity DNA Polymerase and polymerase buffer according to the manufacturer’s instructions (New England BioLabs, NEB). The PCR amplifications were performed using 96 well plates and Veriti™ 96-Well Thermal Cycler (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2020Quote: ... Q5 High-Fidelity DNA Polymerase and polymerase buffer according to the manufacturer’s instructions (New England BioLabs, NEB). The PCR amplifications were performed using 96 well plates and Veriti™ 96-Well Thermal Cycler (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2020Quote: ... pri-miRNA sequences were amplified by PCR using Phusion® High-Fidelity DNA Polymerase (NEB BioLabs, USA) from human embryonic kidney (HEK293T ...
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bioRxiv - Molecular Biology 2020Quote: ... pri-miRNA sequences were amplified by PCR using Phusion® High-Fidelity DNA Polymerase (NEB BioLabs, USA) from human embryonic kidney (HEK293T ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amount of each fragment was mixed with dNTP and Phusion® High-Fidelity DNA Polymerase (NEB) in the absence of primers ...
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bioRxiv - Developmental Biology 2019Quote: ... lin-29a and lin-29b were amplified by PCR using Phusion High-Fidelity DNA Polymerase (NEB, M0530S) from purified genomic wild-type C ...
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bioRxiv - Bioengineering 2019Quote: ... All PCR amplifications were performed using Q5 Hot Start High-Fidelity 2X Master Mix (NEB, Cat: M0494S) unless specified ...
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bioRxiv - Synthetic Biology 2019Quote: ... at a ratio of 1:40 using 0.5 µL of Q5 High-Fidelity DNA Polymerase (NEB, M0491S) in a 50 µL reaction containing 1x Q5 polymerase reaction buffer (NEB ...
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bioRxiv - Microbiology 2019Quote: ... verticillioides with the primers FvGbb2-GFP-F/R using Q5® High-Fidelity DNA Polymerase (NEB: M0491S). The DNA fragment was introduced to the pKNTG plasmid KpnI and HindIII sites via In-Fusion-HD cloning kit (Takara Bio USA ...