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Citations for New England Biolabs :
201 - 250 of 8043 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... and 12.5 µL 2X Hot Start Taq PCR Master Mix (New England Biolabs). Samples were run on a Bio-Rad T100™ Thermal Cycler and cycled for 5 minutes at 95°C ...
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bioRxiv - Genetics 2019Quote: ... and 10 µL 2X Hot Start Taq PCR Master Mix (New England Biolabs). Samples were loaded into a Biorad T100™ Thermal Cycler for 3 minutes at 95°C ...
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bioRxiv - Genomics 2019Quote: ... in a 50⍰ μl reaction with 2× PCR master mix (NEB cat. M0541). PCR cycled as ...
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bioRxiv - Biochemistry 2019Quote: ... PCR was performed using Q5 Host Start High- Fidelity 2× Master Mix (NEB), and 150 ng Genomic DNA was chosen as the template ...
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bioRxiv - Microbiology 2019Quote: ... PCR reactions were performed using Q5® High-Fidelity 2x Master Mix (NEB) for cloning and sequencing otherwise DreamTaq Green PCR Master Mix 2x (Thermo Scientific ...
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bioRxiv - Immunology 2019Quote: ... and enrichment PCR performed with NEBNext Ultra Q5 Master Mix (New England BioLabs). Samples were pooled in equal proportions and size-selected using 0.6x followed by 0.2x of AMPure XP beads (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... and enrichment PCR performed with NEBNext Ultra Q5 Master Mix (New England BioLabs). Samples were pooled in equal proportions and size-selected using 0.6x then 0.2x AMPure beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2021Quote: ... The qRT-PCR assay was performed using Luna Universal qPCR Master Mix (NEB) with a total volume of 20 µl ...
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bioRxiv - Genomics 2021Quote: ... 25 μL NEBNext Hi-Fi 2x PCR Master Mix (NEB (Ipswich, MA, USA) M0541S) ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCRs were performed using Q5® High-Fidelity 2X Master Mix (NEB #M0492L), sfGfp as the template ...
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bioRxiv - Genetics 2021Quote: ... PCR amplicons were generated using Q5® High-Fidelity 2X Master Mix (NEB) using primers PKS12_F2 and PKS12_R2 ...
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bioRxiv - Microbiology 2020Quote: ... The PCR reaction was performed with Q5 High-Fidelity Polymerase Master Mix (NEB) and the product column purified with a QIAquick PCR purification kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... followed by PCR amplification with NEBNext UltraII Q5 Master Mix (New England BioLabs). Libraries were subjected to Illumina deep sequencing (HiSeqV4 SR50) ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were performed with Taq 2X Master Mix (New England BioLabs, M0270L). The thermocycler conditions for Mouse Chk1 and GAPDH PCR product were as follows ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA (20 ng) was PCR amplified using LongAmpTaq 2X master mix (NEB). The PCR amplicons of selected regions were subjected to Sanger sequencing and BLAST analysis to confirm the presence of eight variants using the primers listed in Supplemental Table 1.
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bioRxiv - Microbiology 2022Quote: Phusion High-Fidelity PCR Master Mix with HF Buffer (New England BioLabs #M0530) was used to amplify cDNA around the region of interest ...
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bioRxiv - Microbiology 2022Quote: ... for PCR amplification and the Gibson Assembly Master Mix (New England Biolabs, NEB) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... for PCR amplification and the Gibson Assembly Master Mix (New England Biolabs, NEB) according to the manufacturer instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Libraries were prepared using NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541) with the following conditions ...
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bioRxiv - Microbiology 2022Quote: ... Reverse-transcription PCR was then performed using LunaScript RT Master Mix Kit (NEB) using a gene specific primer ...
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bioRxiv - Microbiology 2022Quote: ... The PCR fragments were assembled using HiFi Assembly Master Mix (New England Biolabs) as per the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... from PCR fragments obtained with Q5 High-Fidelity 2X Master Mix (#M0492L, NEB). eolTRPA1 coding sequence from Texas Rat snake (Elaphe (Pantherophis ...
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bioRxiv - Genomics 2022Quote: ... and PCR amplified for 2 cycles using the NEBNext master mix (NEB M0544L) with Illumina Nextera primers and conditions ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were assembled with NEB Hifi assembly master mix (New England Biolabs) following the standard protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative PCR was performed in duplicate with Luna Universal qPCR Master Mix (NEB) using QuantStudio 5 Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Systems Biology 2023Quote: ... 25 μL of the NEBNext Q5 HotStart HiFi PCR Master Mix (NEB, M0543S), and nuclease-free water to a final volume of 50 μL ...
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bioRxiv - Microbiology 2023Quote: ... for PCR amplification and the Gibson Assembly Master Mix (New England Biolabs, NEB) for insert-plasmid assembly ...
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bioRxiv - Microbiology 2023Quote: ... for PCR amplification and the Gibson Assembly Master Mix (New England Biolabs, NEB) for insert-plasmid assembly ...
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A laboratory framework for ongoing optimisation of amplification based genomic surveillance programsbioRxiv - Genomics 2023Quote: ... Each PCR contained 12.5μL Q5 High Fidelity 2x Master Mix (New England Biolabs), 1.1μL of pooled primers mixes at 10μM (final concentration of each primer was ∼10-11pM) ...
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bioRxiv - Microbiology 2023Quote: ... Linearized pExTra plasmid was prepared via PCR (NEB Q5 HotStart 2X Master Mix) of pExTra01 (Heller et al ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA PCR was performed with using OneTaq® 2X Master Mix (NEB) and used primer pairs were ...
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bioRxiv - Evolutionary Biology 2023Quote: ... All PCR mixtures used Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the specified primers and templates below ...
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bioRxiv - Genetics 2023Quote: ... Amplification was performed with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541s) and Nextera PCR Primers (8 cycles) ...
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bioRxiv - Genomics 2023Quote: ... The PCR was performed using Q5 High-Fidelity 2X Master Mix (NEB, M0492L) with conditions described in Supplementary Table 4.
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bioRxiv - Biophysics 2023Quote: ... and Q5 Hot Start High-Fidelity 2X PCR Master Mix (New England Biolabs). The transcribed region had the following spacings ...
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bioRxiv - Genetics 2022Quote: ... PCRs were performed using Q5 Hot-start 2× master mix (New England Biolabs). Restriction digests were conducted for 2 hours at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR was performed using the Q5 ® Hot Start Master mix (NEB M0491), supplemented with 10 μΜ of each primer and 20 ng of template in a final volume of 25 μl in the following conditions ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative PCR was performed with Luna® Universal qPCR Master Mix (NEB #M3003) and Biorad CFX96 Real time PCR system ...
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bioRxiv - Cell Biology 2024Quote: ... and PCR was performed using Q5® High-Fidelity 2X Master Mix (NEB). The splicing efficiency is monitored using RT-PCR with primers located on two adjacent exons ...
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bioRxiv - Microbiology 2023Quote: ... 25 µl NEBNext High-Fidelity 2X PCR Master Mix (NEB, catalog number M0541S). In each 50 µl reaction ...
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bioRxiv - Neuroscience 2023Quote: ... PCR products were cloned into target constructs using HiFi Master mix (E2621, NEB). All constructs were verified by dideoxynucleotide sequencing (Macrogen) ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed using the Phusion Hot Start Flex 2X Master Mix (NEB) in a GeneAmp PCR system 9700 (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... and amplified and indexed with NEBNext High- Fidelity 2X PCR Master Mix (NEB) and custom primers ...
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bioRxiv - Genetics 2023Quote: ... DNA was amplified using NEBNext Ultra II Q5 PCR Master Mix (NEB, #M0544S) with Index Primers from Illumina (IDT for Illumina DNA/RNA UD Indexes Set A (Illumina ...
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bioRxiv - Genomics 2023Quote: ... using Q5 High-Fidelity PCR master mix with HF buffer (NEB Ipswich, MA) and PCR primers containing the T7 promoter sequence (Forward ...
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bioRxiv - Microbiology 2023Quote: PCR was performed using Q5 High-Fidelity 2X Master Mix (New England Biolabs) as per manufacturer’s instructions with the following primers ...
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bioRxiv - Synthetic Biology 2023Quote: ... Phusion® High-Fidelity PCR Master Mix with HF Buffer (New England BioLabs) was used to screen C ...
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bioRxiv - Developmental Biology 2023Quote: ... 10.0 μl NEBNext High-Fidelity 2×PCR Master Mix (New England Biolabs, M0541S), 0.25 μl 100 μM PCR Index 1 ...
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bioRxiv - Microbiology 2023Quote: ... The PCR was performed using Q5 2× Master Mix (New England Biolabs, M0492S), 6 µL of cDNA synthesis reaction ...
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bioRxiv - Developmental Biology 2024Quote: ... and screened by colony PCR with OneTaq Quick-Load 2X Master Mix (NEB). Positive colonies were cultured overnight (o/n ...