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Citations for New England Biolabs :
401 - 450 of 8043 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2023Quote: ... We performed 5 PCR enrichment with 15 cycles (30 ng DNA input, NEB Phusion High-Fidelity PCR Master Mix) for each library to increase fragment diversity ...
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bioRxiv - Genetics 2023Quote: ... The PCR reaction was performed using NEBNext® High-Fidelity 2× PCR Master Mix (New England BioLabs, catalog #M0541) with a reaction volume of 10 ul including 5 ul 2× PCR Master Mix ...
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bioRxiv - Microbiology 2023Quote: ... The 30 μl PCR reaction system contained 15 μl of Phusion high-fidelity PCR Master Mix (New England Biolabs), 0.2 μM forward and reverse primers ...
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bioRxiv - Genomics 2023Quote: ... All PCR reactions were performed with 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 μM of forward and reverse primers ...
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bioRxiv - Plant Biology 2022Quote: ... were analyzed by Quantitative real time PCR (qRT PCR) using Luna® Universal qPCR Master Mix (New England BioLabs) in a QuantStudio® 5 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... ATAC-seq libraries were prepared by ∼8 cycles of PCR using NEBNext High Fidelity 2X PCR Master Mix (NEB) and primers containing Illumina barcodes (Buenrostro et al ...
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bioRxiv - Genomics 2023Quote: ... Accessible DNA libraries were amplified by PCR using NEBNext High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA), custom Nextera PCR primers as previously described (29) ...
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bioRxiv - Plant Biology 2024Quote: ... The sequencing libraries were prepared by PCR amplification using the NEBNext PCR master mix (New England Biolabs, no. M0541S). The amplified libraries were purified with AMPure beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: ... Addgene cat#52961) by PCR amplification using NEB Next High-Fidelity PCR Master Mix (New England Biolabs, cat#M0541S). The PCR product was digested with Esp3I (BsmBI ...
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bioRxiv - Genetics 2019Quote: PCRs were performed with the Q5 Hot-start 2x master mix (New England Biolabs (NEB)) and cloning was performed using the In-Fusion HD cloning kit (Takara Bio ...
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bioRxiv - Developmental Biology 2020Quote: ... fusion PCR and added them to the pWPXL vector with Gibson Assembly Master Mix (NEB). We then constructed final transfer vectors by inserting target cDNA after the P2A using Gibson assembly ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR products were produced with the Q5 High-Fidelity 2X Master Mix (New England Biolabs). All inserts were verified by sequencing ...
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bioRxiv - Immunology 2022Quote: ... Realtime PCR is conducted following manufacturer standard protocol (Luna Universal qPCR Master Mix, NEB Inc). Sybr green RT-PCR primers used in this study is listed in Table 1.
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bioRxiv - Genomics 2019Quote: ... and the NEBNext High Fidelity 2X PCR Master Mix (New England Biolabs; Ipswich, MA, USA). PCR reactions were cleaned up with one volume AmpureXP beads ...
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bioRxiv - Genomics 2019Quote: ... SIC integration sites were enriched by PCR (NEBNext Ultra Q5 Master Mix, New England Biolabs) using 12 µL of the eluate with suitable pairs of adapter- and SIC-specific primers ...
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bioRxiv - Genetics 2019Quote: ... but using NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs, Ipswich, U.S.). Finally ...
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bioRxiv - Genetics 2020Quote: ... PCR amplification was performed using Phusion 2X Master Mix HotStart Flex (New England Biolabs M0536L), 10µM primer pair (see Key Resources Table Oligonucleotides) ...
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bioRxiv - Genomics 2020Quote: ... After adding 30 µl PCR MM [25 µl 2x LongAmp Taq 2X Master Mix (NEB), 0.2 µl 100 mM forward primer ...
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bioRxiv - Bioengineering 2020Quote: ... PCR was performed using Q5® High-Fidelity 2× Master Mix (New England Biolabs, US). The point mutation in dCas9 to create H840A Cas9n was made using Q5® Site-Directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Bioengineering 2020Quote: ... or colony PCR using Q5® High-Fidelity 2× Master Mix (New England Biolabs, US) if a chromosomal region was targeted ...
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bioRxiv - Neuroscience 2020Quote: The introns were PCR amplified using Q5 Hot Start 2x Master Mix (New England BioLabs) with primers designed to contain homology arms to their respective targets ...
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bioRxiv - Microbiology 2021Quote: ... and PCR carried out using Q5® High-Fidelity 2X Master Mix (New England Biolabs) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... The template was PCR amplified (Taq 2x master mix, NEB M0270; Bridge primers; 15 cycles) and size selected by 2% agar gel.
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bioRxiv - Cancer Biology 2020Quote: ... and 25 μl of NEBNext High Fidelity PCR Master 2X Mix (New Englarnd Biolabs, M0541L). The primer sequences and their description ...
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bioRxiv - Cell Biology 2021Quote: ... from all the samples and amplified by PCR using Q5 master mix (New England Biolabs) and forward-TGTGTCAATGGAACTCTTGAC and reverse-TCGTTTCGACACCGAAGCAG primers.
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bioRxiv - Immunology 2022Quote: ... VP2 and VP3 with high-fidelity Phusion HiFi PCR Master Mix (NEB, Ipswich, MA, USA) using specific primers (S6 Table) ...
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bioRxiv - Genomics 2022Quote: ... and Phusion® High-Fidelity PCR Master Mix with HF buffer (New England Biolabs Inc). The amplification was performed in 50 μL PCR reactions that contained:
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bioRxiv - Cell Biology 2022Quote: ... RAD18 knockout positive cells were confirmed with PCR using Q5 High Fidelity Master Mix (NEB), sanger sequencing ...
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bioRxiv - Immunology 2020Quote: ... Each PCR reaction was performed using Q5 Hot Start High Fidelity 2X Master Mix (NEB). For the first round of PCR ...
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bioRxiv - Neuroscience 2021Quote: ... PCR products were produced with the Q5 High-Fidelity 2X Master Mix (New England Biolabs). All inserts were verified by sequencing.
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bioRxiv - Neuroscience 2020Quote: ... 25 μl of the NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs, M0541S), and 5 µL of the Illumina PCR Primer Cocktail (PPC ...
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bioRxiv - Genomics 2022Quote: ... Each PCR#2 reaction contained 25 µL of Q5 High-Fidelity 2X Master Mix (NEB), 2.5 µL of a unique Nuc PCR#2 Fwd Primer (10 µM) ...
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bioRxiv - Genomics 2022Quote: ... Each “PCR#1” reaction contained 25 µL of Q5 High-Fidelity 2X Master Mix (NEB), 2.5 µL of Nuc PCR#1 Fwd Primer (10 µM) ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were produced with the Q5 High-Fidelity 2x Master Mix (New England Biolabs). Some fragments were ordered as gBlocks at IDT ...
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bioRxiv - Immunology 2022Quote: PCRs were done with the Q5 Hot-start 2× master mix (New England BioLabs, NEB), and cloning was performed using the Gibson Assembly 2× Master Mix (NEB ...
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bioRxiv - Immunology 2022Quote: PCRs were done with the Q5 Hot-start 2× master mix (New England BioLabs, NEB), and cloning was performed using the Gibson Assembly 2× Master Mix (NEB ...
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bioRxiv - Genomics 2019Quote: cDNA was amplified with NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs) using input amounts determined from qPCR ...
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bioRxiv - Genomics 2019Quote: cDNA was amplified with NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs) using volumes determined from qPCR distributed across 5 replicates each ...
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bioRxiv - Genomics 2019Quote: ... gDNA was amplified with NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs) with a biotinylated reverse-transcriptase primer Creb_Hand_RT_3 and a biotinylated primer specific to luciferase (Creb_Luc_Seq_R) ...
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bioRxiv - Microbiology 2019Quote: ... libraries were amplified with NEBNext High-Fidelty 2x PCR Master Mix (NEB, cat. number: M0541), the RP1 common primer and a uniquely selected index primer ...
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bioRxiv - Molecular Biology 2021Quote: ... and the resulting library was PCR amplified using Q5 Ultra II Master Mix (NEB, # M0544S). The library was size selected by agarose gel electrophoresis and column purified (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... 25□µL NEBNext High-Fidelity 2X PCR master mix (New England Biolabs Inc., Ipswich, MA), 5□µL of each index primer ...
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bioRxiv - Microbiology 2020Quote: ... Both PCR products were then assembled using NEBuilder® HiFi DNA Assembly Master Mix (NEB), resulting in VHp693.
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bioRxiv - Developmental Biology 2021Quote: ... a PCR was undertaken on yw67 fly gDNA using a Q5 polymerase master mix (NEB) and the following primer pair (preceded by the T7 promoter sequence ...
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bioRxiv - Genomics 2021Quote: ... then 20 µl of 2X PCR master mix (New England Biolabs, New England Biolabs, M0541S) was added to the samples ...
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bioRxiv - Genomics 2021Quote: ... then 20 µl of 2X PCR master mix (New England Biolabs, New England Biolabs, M0541S) was added to the samples ...
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bioRxiv - Cancer Biology 2021Quote: ... sgRNA inserts were amplified with NEBNext High-Fidelity 2X PCR Master Mix (New England BioLabs). Samples were then pooled in equimolar concentrations ...
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bioRxiv - Genetics 2019Quote: PCRs were performed with the Q5 Hot-start 2x master mix (New England Biolabs (NEB)) and cloning was performed using the In-Fusion HD cloning kit (Takara Bio ...
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bioRxiv - Genomics 2020Quote: ... Using primers 642F and 643R and NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541L), PCR was run for 10 cycles with the following parameters ...
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bioRxiv - Genetics 2019Quote: ... PCR products were produced with the Q5 High-Fidelity 2X Master Mix (New England Biolabs). All inserts were verified by sequencing ...