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Citations for New England Biolabs :
151 - 200 of 8043 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Quantitative PCR was performed with Luna® Universal qPCR Master Mix (NEB). All procedures were carried out according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... Phusion High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA, USA) was used for all PCR steps ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic PCRs were performed using Hot Start Taq 2x Master Mix (NEB). PTGER2 primers that bind to conserved sequences in both human and mouse were used as internal control ...
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bioRxiv - Developmental Biology 2024Quote: ... 2uL 10uM uniquely barcoded i7 primer and 25uL PCR master mix (NEB Phusion® High-Fidelity PCR Master Mix with HF Buffer ...
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bioRxiv - Genomics 2024Quote: RT-PCR was conducted using Quick-Load Taq 2X Master Mix (NEB). For molecular cloning applications Q5 High-Fidelity DNA polymerase (NEB ...
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bioRxiv - Genomics 2023Quote: ... and amplified using NEBnext High-Fidelity 2x PCR Master Mix (NEB, M0541L) and custom indexed primers68 ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR assays were performed using the Luna Universal qPCR Master Mix (NEB) in a MasterCycler RealPlex 4 (Eppendorf) ...
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bioRxiv - Pathology 2024Quote: ... qRT PCR was performed with the LUNA Universal qPCR Master Mix (NEB) following manufacturer’s instructions and 1 µl of cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... Library was prepared with NEBNext High-Fidelity 2X PCR Master Mix (NEB) and SYBR Green I (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... and amplified using high-fidelity 2X PCR Master Mix (New England Biolabs) using primers with standard ATAC-seq barcodes (Buenrostro et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 µl of NEBNext HiFi 2x PCR Master Mix (New England BioLabs) was mixed with 21 µl of the CUT&Tag DNA ...
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bioRxiv - Developmental Biology 2024Quote: ... Eluted DNA was amplified using NEBNext Ultra II PCR Master Mix (NEB) and purified using AMPure XP beads ...
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bioRxiv - Bioengineering 2024Quote: ... PCR products were assembled using NEB Hifi DNA Assembly Master Mix (NEB) according to the manufacturer instructions ...
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bioRxiv - Genetics 2021Quote: ... and libraries were generated by PCR with NEBNext High Fidelity 2X PCR Master Mix (NEB M0541). Library size was confirmed on a BioAnalyzer prior to sequencing on the NextSeq 550 (40bp read length ...
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bioRxiv - Molecular Biology 2022Quote: ... Site-directed mutagenesis was performed by PCR with NEBNext High-Fidelity 2x PCR master mix (NEB) using overlapping primers with 15 nt overhangs carrying the desired mutation (IDT).
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bioRxiv - Genetics 2022Quote: ... Each PCR consisted of: 20 µL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 2 µL 10 µM i5 indexing adapter ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... All PCR reactions were carried out with Phusion High-Fidelity PCR Master Mix (New England Biolabs). The PCR products were mixed with an equal volume of 1× loading buffer (containing SYB green ...
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bioRxiv - Molecular Biology 2022Quote: ... Post-capture PCR was performed with the NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541S) for 14-20 cycles ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR reaction was performed using NEBNext High Fidelity PCR Master Mix (NEB, catalog no. M0541L) for 20 cycles ...
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bioRxiv - Biochemistry 2023Quote: ... Each PCR consisted of: 20 μL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 1 μL 10 μM forward primer ...
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bioRxiv - Synthetic Biology 2023Quote: ... General PCR for cloning was performed using the NEBNext PCR master mix (NEB cat no. M0541L), with 500 nM of forward and reverse primers ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were carried out using the Phusion High-Fidelity PCR Master Mix (New England Biolabs). The PCR products were selected by 2% agarose gel electrophoresis ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA libraries underwent PCR amplification (NEB-Next High-Fidelity 2x PCR Master Mix, New England Biolabs), indexing using previously described primers (Buenrostro et al ...
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bioRxiv - Genetics 2024Quote: ... and libraries were generated by PCR with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541). Prior to sequencing ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCRs were carried out using the Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB) in a total volume of 20ul ...
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bioRxiv - Synthetic Biology 2024Quote: All PCRs were carried out using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB), gel extractions using the QIAquick Gel Extraction Kit (QIAGEN ...
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bioRxiv - Molecular Biology 2022Quote: ... Beads were resuspended in 25 μl cDNA amplification mix (1X Phusion HF PCR Master Mix (NEB, #M0531L) and 0.5uM P5 and P7 short primer mix (see Supplementary Table 2) ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were amplified with i5/i7 Nextera primer index mix and Q5 PCR Master Mix (NEB #M0492L): 5 min 72°C ...
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Genomic dissection and mutation-specific target discovery for breast cancer PIK3CA hotspot mutationsbioRxiv - Genomics 2024Quote: ... PCR mix and thermocycler conditions were set according to the Phusion Master Mix protocol provided by NEB. PCR products were measured and visualized using a D5000 ScreenTape System (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... 2X Master Mix (NEB) and cDNA primer (cPRM ...
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bioRxiv - Genetics 2021Quote: ... RT-PCR was conducted using the Q5 High-Fidelity 2X Master Mix (NEB). All primers used in the current study are available in Table S1.
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were prepared using NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541) with the following conditions ...
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bioRxiv - Genomics 2022Quote: ... and PCR was performed using enrichment primers and Phusion HF Master Mix (NEB) for 12 cycles ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR was completed using the Luna polymerase master mix (NEB, cat# M3003) using gene-specific primers (Table S1) ...
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Pancreatic progenitor epigenome maps prioritize type 2 diabetes risk genes with roles in developmentbioRxiv - Genomics 2020Quote: ... Libraries were amplified using NEBNext High-Fidelity 2X PCR Master Mix (M0541, NEB) with primer extension at 72°C for 5 minutes ...
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bioRxiv - Genomics 2020Quote: ... Libraries were amplified using NEBNext High-Fidelity 2X PCR Master Mix (M0541, NEB) with primer extension at 72°C for 5 minutes ...
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bioRxiv - Genomics 2019Quote: ... or the Q5 Hot Start HiFi PCR master mix (New England Biolabs E6625AA) (7 - 10 cycles) ...
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bioRxiv - Cancer Biology 2021Quote: ... Sequencing libraries were prepared using NEBNext HiFi 2× PCR Master Mix (NEB, M0541L) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Libraries were prepared with NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541L), following standard protocol ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed using 2x LongAmp Taq Master Mix (New England Biolabs, M0287S) to select full- length transcripts followed by a second purification step using Agencourt beads (as described above) ...
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bioRxiv - Microbiology 2021Quote: ... for PCR amplification and the Gibson Assembly Master Mix (New England Biolabs, NEB) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... for PCR amplification and the Gibson Assembly Master Mix (New England Biolabs, NEB) according to the manufacturer instructions ...
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bioRxiv - Biochemistry 2022Quote: ... and PCR library amplification (KAPA library amplification primers; NEB Ultra 2× master mix), followed by double-sided SPRI size selection and a final 0.9× SPRI purification ...
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bioRxiv - Developmental Biology 2022Quote: ... using the NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs #M0451S) with Nextera DNA CD Indexes (Illumina #20015882) ...
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bioRxiv - Neuroscience 2022Quote: ... 25 μl NEBNext High-Fidelity 2× PCR Master Mix (New England Biolabs, M0541S), 9 μl of unique ...
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bioRxiv - Genetics 2022Quote: Conversion tracts were PCR amplified using OneTaq 2x Master Mix (New England Biolabs). Noncrossover recombination products were amplified using forward primer DLO822 (5’-ATTTTAACCCTTCGGGGTACG-3’ ...
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bioRxiv - Biophysics 2022Quote: ... for PCR amplification and isothermal assembly using 2X Gibson master mix (NEB E2611) before transformation into NEB Stable or 5-alpha chemically competent cells.
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bioRxiv - Developmental Biology 2021Quote: ... and then amplified with NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L). Libraries were sequenced on HiSeq 125bp paired-end platform.
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bioRxiv - Immunology 2022Quote: ... Quantitative RT-PCR using Luna® Universal qPCR Master Mix (New England BioLabs) was performed with the following cycling conditions on a CFX384 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Genetics 2019Quote: ... and 10 µL 2X Hot Start Taq PCR Master Mix (New England Biolabs). The cycling conditions in the Bio-Rad T100™ Thermal Cycler were ...