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Citations for New England Biolabs :
101 - 150 of 8043 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 25 µL NEBNext HiFi 2× PCR Master Mix (New England BioLabs) was added to the DNA mixture ...
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bioRxiv - Genomics 2021Quote: ... Tagmented DNA fragments were amplified by adding 12 µL PCR master mix composed of 11 µL Q5 High-Fidelity 2x Master Mix (New England Biolabs, #M0492) and 0.5 µL each of 10 mM Nextera i5 and i7 index primers ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were generated by PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (NEB). The resulting libraries were multiplexed and sequenced in a HiSeq 4000 pair-end lane producing 100M of 49-bp pair end reads per sample.
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bioRxiv - Microbiology 2019Quote: ... Amplicon PCR was performed using the Phusion High-Fidelity PCR Master Mix (New England Biolabs) and previously described barcoded primers37 to amplify the V1-V3 region of bacterial 16S rDNA ...
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bioRxiv - Cancer Biology 2019Quote: ... All PCRs were performed with Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB).
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bioRxiv - Plant Biology 2021Quote: ... PCR was performed using High-Fidelity Phusion PCR Master Mix (NEB, Frankfurt am Main, Germany) (initial denaturation (98 °C ...
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bioRxiv - Cell Biology 2021Quote: ... The DNA segments were PCR amplified (Phusion High-Fidelity PCR Master Mix, NEB, UK, #M0531) and fused with Gibson recombination cloning method (Gibson Assembly Master Mix ...
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bioRxiv - Developmental Biology 2019Quote: ... and PCR was amplified with high-fidelity 2X PCR Master Mix (New England Biolabs M0541). One-third of the maximum fluorescent intensity was used to determine the additional cycles ...
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bioRxiv - Genetics 2019Quote: ... and amplified by PCR using Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) to produce sub-pools for Gibson assembly (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... and PCR was amplified with high-fidelity 2x PCR Master Mix (New England Biolabs M0541). One-third of the maximum fluorescent intensity during a qPCR trial run was used to determine the additional cycles for library prep ...
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bioRxiv - Bioengineering 2022Quote: Genomic PCR was performed using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs) with the primers listed in Supplementary Table 1 ...
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bioRxiv - Systems Biology 2023Quote: ... PCR amplification was carried out in a reaction mixture containing PCR Master Mix (NEB, M0543S), 0.5 μM of each primer ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were generated by PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (NEB). The resulting libraries were multiplexed and sequenced in a HiSeq 4000 paired-end lane ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR was performed with 2x NEBNext High-Fidelity PCR Master Mix (New England Biolabs, M0541S) and 10 μM primers (Table S2 ...
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bioRxiv - Genomics 2023Quote: ... The outward PCR experiments were performed using NEBNext High-Fidelity 2X PCR Master Mix (NEB). For a 50 µl reaction ...
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bioRxiv - Genomics 2023Quote: ... pooled oligonucleotides were amplified via PCR using NEBNext 2X Hi-Fi PCR Master Mix (NEB) and primers:
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were generated by PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (NEB). The resulting libraries were purified using MinElute PCR Purification Kit (Qiagen) ...
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bioRxiv - Developmental Biology 2023Quote: ... After samples were PCR-amplified using 1×NEBNext High-Fidelity PCR Master Mix (NEB, M0541S). Subsequent libraries were purified with the MinElute PCR Purification Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... 25 μL 2x NEBnext High-Fidelity PCR Master Mix (New England Biolabs) and 10 μL nuclease-free distilled H2O (ThermoFisher Scientific) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and mixed with the PCR amplicon in Gibson Assembly Master Mix (NEB; protocol as described by the manufacturer) ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed with Taq 2X Master Mix (New England BioLabs, Maine), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR genotyping was performed using Q5 High-Fidelity 2X Master Mix (NEB) with the primer pairs listed in Table 2 ...
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bioRxiv - Synthetic Biology 2019Quote: ... DNA was amplified using Phusion High-Fidelity PCR Master Mix (NEB, M0531S) and the following conditions ...
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bioRxiv - Bioengineering 2019Quote: ... Target sites were amplified using High Fidelity 2X PCR Master Mix (NEB). Primers used for PCR are listed in Supplementary Materials ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and/or Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) (Table S2) ...
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bioRxiv - Neuroscience 2021Quote: ... PCRs were performed using OneTaq 2X Master Mix with Standard Buffer (NEB) using the following primers ...
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bioRxiv - Microbiology 2020Quote: ... PCR (NEBNext Ultra II Q5 ® Master Mix, NEB, Ipswich, MA, USA) was performed with a gene-specific forward primer designed 700-nt upstream of the apparent boundary between the SARS-CoV-2 genome body and the putative poly-A tail ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were indexed using NEBNext High-Fidelity PCR Master Mix (NEB M0541S) for 11 cycles (size-matched input ...
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bioRxiv - Biochemistry 2020Quote: ... PCR amplifications were carried out using 2 x Q5 Master Mix (NEB), with cycling times and temperatures according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Amplicons were generated using Q5 PCR Master Mix (NEB, Ipswich, MA, USA), and the resulting PCR products were cleaned using a NucleoSpin Gel and PCR Clean-Up kit (Macherey-Nagel ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... PCR amplification was performed using Q5 hotstart high fidelity master mix (NEB) with respective primers and 2 ng of Plasmid pKR72 as template DNA ...
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bioRxiv - Microbiology 2022Quote: ... Real-time PCR was measured using the SYBR green master mix (NEB). Cq was used to calculate the relative expression of the target gene by the 2-ΔΔ Cq method ...
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bioRxiv - Neuroscience 2020Quote: ... plus 25 μl NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L) was added to 20 μl of purified transposed DNA ...
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bioRxiv - Genetics 2022Quote: ... 25 µL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 20 µL nuclease-free water ...
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bioRxiv - Genomics 2022Quote: ... Eluted DNA was amplified using NEBNext Ultra II PCR Master Mix (NEB) and purified using AMPure XP beads ...
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bioRxiv - Genetics 2020Quote: ... 25 μL of High-Fidelity 2X PCR Master Mix (New England BioLabs), 2.5 uL of indexing primer and 10 μL of DDW were added to the 10 μL of purified DNA and the DNA was amplified five cycles using the following PCR program ...
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bioRxiv - Developmental Biology 2020Quote: ... and amplification was performed in 1 × next PCR master mix (NEB, M0541S) and 1.25 μM of custom Nextera PCR primers 1 and 2 with the following PCR conditions ...
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bioRxiv - Developmental Biology 2021Quote: ... and amplified using NEBNext® High-Fidelity 2X PCR Master Mix (NEB). The number of cycles was estimated by qPCR ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... For all PCR reactions Q5 High-Fidelity 2X Master Mix (NEB M0492L) was used ...
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bioRxiv - Microbiology 2020Quote: ... 5 μL 2x NEBnext High-Fidelity PCR Master Mix (New England Biolabs) and 2.9 μL nuclease-free distilled H2O (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... A PCR cocktail consisting of NEBNext Ultra II Q5 master mix (NEB) and Illumina TruSeq PCR primers (RP-1 ...
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bioRxiv - Genomics 2021Quote: ... For Smartseq3xpress with NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB), PCR mastermix consisted of (1x NEBNext Q5 Hot Start HiFi PCR Master Mix ...
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bioRxiv - Bioengineering 2022Quote: ... then PCR amplified using the Q5 High-Fidelity 2X Master Mix (NEB). For the tetrahedra with 5 and 7 helical turns per edge ...
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bioRxiv - Microbiology 2022Quote: ... qRT-PCR was performed using Luna Universal qPCR Master Mix (NEB; M3003) with SYBR Green ...
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bioRxiv - Genomics 2022Quote: ... Eluted DNA was amplified using NEBNext Ultra II PCR Master Mix (NEB) and purified using AMPure XP beads ...
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bioRxiv - Genomics 2022Quote: ... and 20 μL NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L) were added to each well ...
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bioRxiv - Microbiology 2023Quote: ... A polymerase chain reaction (PCR) amplification using OneTaq Master Mix (NEB, M0482) was performed on C ...
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bioRxiv - Developmental Biology 2023Quote: ... 2uL 10uM uniquely barcoded i7 primer and 25uL PCR master mix (NEB Phusion® High-Fidelity PCR Master Mix with HF Buffer ...
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bioRxiv - Zoology 2023Quote: ... 5 µl consisting of 1x OneTaq® PCR master mix (NEB, USA), 0 ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative PCR was performed with Luna® Universal qPCR Master Mix (NEB). All procedures were carried out according to manufacturer instructions ...