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Citations for New England Biolabs :
201 - 250 of 10000+ citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The tcaA promoter region was also digested with EcoRI and SmaI and ligated into pSB2019 using T4 DNA ligase (NEB). This was transformed into DC10B37 ...
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bioRxiv - Molecular Biology 2023Quote: ... The promoters and CpG islands of the three studied species were first PCR amplified with Q5 DNA polymerase (New England BioLabs) using gene-specific primers (Table 2 ...
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bioRxiv - Plant Biology 2022Quote: All ObANS genes were inserted into the pBI121 plasmid under the control of the constitutive Cauliflower mosaic virus 35S promoter using the SacI and XbaI restriction enzymes (Thermo-scientific) and the NEBuilder ligation system (NEB). The plasmids were transformed into Agrobacterium tumefaciens strain GV3101 using electrotransformation and positive colonies were selected with kanamycin ...
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bioRxiv - Plant Biology 2022Quote: PCR-generated fragments of the CNX1 and CNX2 genomic regions lacking stop codons and including the 1-kbp promoter regions were obtained using Phusion HF DNA polymerase (New England Biolabs), inserted into pENTR-2B ...
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bioRxiv - Bioengineering 2024Quote: ... The TSA1 promoter was amplified by PCR from genomic DNA obtained from the X-33 strain using Q5 polymerase (New England Biolabs) and primers TSA1_BstBI_Fw and TSA1-GFP_Rv_KpnI ...
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bioRxiv - Cell Biology 2024Quote: ... or N-terminus of PEmax (PEmax-hP53DD-P2A-hMLH1dn) under the constitutive expression from a CMV promoter using Hifi DNA assembly (NEB). To generate the other two all-in-one prime editor with linkages in between the three components ...
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bioRxiv - Cancer Biology 2024Quote: ... and two BsmBI Type IIS restriction enzyme sites was cloned into the 3’ end of the bovine U6 promoter using Gibson Assembly (NEBuilder HiFi, NEB) (see Supplementary Figure 1a) ...
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bioRxiv - Microbiology 2024Quote: ... 2007) onto empty pBSV2G and replaced the flaB promoter on pcon++flgV using NEBuilder HiFi DNA Assembly (New England Biolabs) to create pind and pind+flgV ...
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bioRxiv - Molecular Biology 2024Quote: ... were generated by extending a common sgRNA(F+E) sequence with gene-specific crRNA sequence downstream of a T7 promoter by PCR with Phusion polymerase (NEB). PCR product was gel extracted and sgRNA was in vitro transcribed using the MegaShortScript™ T7 Transcription Kit (Invitrogen AM1354 ...
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bioRxiv - Evolutionary Biology 2024Quote: Plasmids carrying the gfp reporter under the control of different versions of hisJ promoter were constructed using the NEBuilder HiFi DNA Assembly (NEB). Primers used to amplify the backbone or the promoter regions from the evolved bacterial lines are reported in the primer list ...
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bioRxiv - Microbiology 2024Quote: ... the kanamycin resistance cassette was replaced with a chloramphenicol resistance cassette using ITA and the region downstream of the promoter was deleted using site-directed mutagenesis using KLD enzyme mix (NEB). The pEMH120 empty vector was constructed from a pTGR5 (88 ...
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bioRxiv - Neuroscience 2023Quote: T7 promoter was inserted upstream of the dual-luciferase coding sequence by using NEBuilder HiFi DNA Assembly (New England Biolabs). Verified plasmid DNAs were digested with restriction enzymes into linear double-stranded DNA templates ...
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bioRxiv - Molecular Biology 2023Quote: ... and a synthetic 391 bp double-stranded DNA fragment encoding 5′-(1st gRNA/scaffold/H1 promoter/2nd gRNA)-3′ was inserted using the NEBuilder HiFi assembly system (NEB). Synthetic DNA fragments were ordered from Genewiz and sequences are listed in Table S6 ...
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bioRxiv - Cell Biology 2024Quote: ... under a CamKII promoter were both restriction digested with AgeI and the HALO protein was ligated with Quick Ligase (NEB) into the ER-GCaMP yielding the CamKII ER-Halo-GCaMP6-150 ...
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Sphingosine induction of the Pseudomonas aeruginosa hemolytic phospholipase C/sphingomyelinase, PlcHbioRxiv - Microbiology 2023Quote: The allelic exchange vector for mutation of the SphR binding site in the cerN promoter was built using HiFi assembly (NEB) from two PCR products amplified from PA14 genomic DNA and HindIII and KpnI cut pMQ30 ...
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bioRxiv - Biophysics 2022Quote: ... were ordered from Integrated DNA Technologies (IDT), (Coralville, Iowa) as gBlock gene fragments downstream to a T7 promoter and flanked by EcoRI (New England Biolabs (NEB),Ipswich ...
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bioRxiv - Genomics 2022Quote: A second-generation lentiviral transfer plasmid encoding expression of EF1ɑ promoter - CAR-P2A-mCherry (21) was digested with XbaI & MluI (New England Biolabs) to drop out the CAR-P2A-mCherry transgene ...
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bioRxiv - Plant Biology 2022Quote: ... the UBQ10 promoter was removed from pUB-RFP-DEST through digestion with restriction endonucleases PspXI and PmeI (New England Biolabs) and the vector subsequently re-ligated using Klenow polymerase (DNA Polymerase I ...
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bioRxiv - Molecular Biology 2023Quote: ... and a synthetic 391 bp double-stranded DNA fragment encoding 5′-(1st gRNA/scaffold/H1 promoter/2nd gRNA)-3′ was inserted using the NEBuilder HiFi assembly system (NEB). Synthetic DNA fragments were ordered from Genewiz and sequences are listed in Supplementary Table 3 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... so that the minimal promoter and EGFP could be reintroduced via a sticky end ligation (T4 DNA Ligase, NEB M0202). This library was transformed and purified ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplified yciF (with Strep II tag on C-terminal) and pQE60 plasmid (containing an IPTG inducible T5 promoter) were digested with BamHI and EcoRI (New England Biolabs) and purified by gel extraction (MinElute gel extraction kit ...
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bioRxiv - Neuroscience 2023Quote: Control and DRP1 gRNA were cloned into a plasmid containing a U6 promoter using BstXI and BlpI restriction enzymes (NEB) (gifted by Dr ...
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bioRxiv - Molecular Biology 2023Quote: ... under its native promoter was cloned into the plasmid pACYC184 (p15A origin of replication) using Gibson assembly (New England Biolabs) generating plasmid JW371 ...
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bioRxiv - Cell Biology 2023Quote: ... flag tag and SunTag reporter were cloned from the SINAPs construct to replace the CMV promoter and tdTomato reporter of the tdTomato-FL2 plasmid using NEBuilder HiFi DNA Assembly technology (NEB).
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bioRxiv - Developmental Biology 2023Quote: ... we cloned the Grx1-roGFP2 sequence from the “pLPCX mito Grx1-roGFP2” plasmid into a lentiviral vector under the EF1 promoter “pCDH-EF1” using Gibson Assembly (NEB) (primers described in Table 1) ...
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bioRxiv - Plant Biology 2024Quote: ... and rbcS-E9 enhancers and the synthetic enhancers syn1–11 were cloned upstream of the 35S minimal promoter in pDL by Golden Gate cloning using BsaI-HFv2 (NEB). The synthetic enhancers were ordered as synthesized DNA fragments.
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bioRxiv - Plant Biology 2024Quote: ... and enhancer fragment combinations were cloned upstream of the 35S minimal promoter by Golden Gate cloning using BsaI-HFv2 (NEB). The resulting libraries were bottlenecked to yield 10–20 barcodes per enhancer.
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bioRxiv - Systems Biology 2024Quote: Each of the selected eight promoters were amplified by PCR and individually inserted by Gibson assembly (#E2611S; New England Biolabs) into the reporter vector ...
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bioRxiv - Cancer Biology 2024Quote: ... modified with an EF1a promoter and a Basticidin resistance gene using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). Specific primers and NEBuilder HiFi DNA Assembly Master Mix were used to introduce the exon E6a or the R238W mutation into the canonical sequence of NT5C2.
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bioRxiv - Immunology 2024Quote: ... DDO268B and the control X region expressing plasmid (with a T7 promoter) were linearized and in vitro transcribed with the Hi Scribe T7 (NEB). RNA products were DNase-treated and precipitated with LiCl ...
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bioRxiv - Systems Biology 2024Quote: ... The fae genes were then integrated into the pAWP78 vector under P89 promoter by Gibson assembly using NEBuilder (NEB Labs) and transformed into E.coli DH10B competent cells ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The 1392-bp region comprising the E9 region was PCR amplified from genomic DNA and cloned upstream of the DPYD promoter using KpnI and SacI sites (New England Biolabs). A vector containing the A allele of rs4294451 within E9 was confirmed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... derived from Pseudomonas aeruginosa) under the control of a T7 RNA polymerase promoter was transformed into Escherichia coli strain BL21-DE3(NEB). After culture and induction in the presence of kanamycin ...
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bioRxiv - Biochemistry 2024Quote: ... A pTrc99a plasmid backbone containing WT CpxA and a mCherry reporter gene under control of CpxAR-dependent promoter pSpy was amplified with Phusion polymerase (New England Biolabs) to produce linear vector with homology arms for assembly with the dsDNA inserts ...
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bioRxiv - Microbiology 2024Quote: ... aur or sspA genes were amplified with their native promoter and ribosomal binding site (Supp Table 6) using Q5 polymerase (NEB). Constructs were generated in the expression plasmid pCT digested with SacI and EcoRI and Antarctic phosphatase treated (NEB ...
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bioRxiv - Biochemistry 2024Quote: A fluorescein (Flc)-labeled 159 bp DNA fragment containing the glnA gene promoter was generated by PCR-amplification with Deep Vent DNA polymerase (New England Biolabs), the primer pair 5’-(Flc)CACAACCAGGAACTGAAGAC-3’ and 5’-(Flc)CGCCTGCAAGATTTCGTTAC-3’ ...
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bioRxiv - Developmental Biology 2024Quote: ... The Ace2N-mNeon fragments and the promoter-containing backbone were then assembled using Gibson Assembly (Gibson Assembly Master Mix, NEB). The final assembly was transformed into NEB® 5-alpha Competent cells ...
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bioRxiv - Cell Biology 2020Quote: ... Full-length 265 nt 5’ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Molecular Biology 2021Quote: ... Library preparation from human islets total RNA (200ng) was performed using a NEBNext Low Input RNA Library Prep kit (NEB). Sequencing was performed on a HiSeq4000 using 75 bp paired end reads according to Illumina specifications ...
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bioRxiv - Molecular Biology 2021Quote: ... Full-length 265 nt 5′ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Developmental Biology 2020Quote: ... We previously generated a human LRP5-V5 plasmid [32] and we used the Q5 site-directed mutagenesis kit (New England Biolabs) with forward primer (CTCAATGGCACATCCCGG ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of total RNA was depleted of rRNA using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) prior to cDNA synthesis primed with random hexanucleotide oligonucleotides (Random Primer 6, NEB). Sequencing library construction was carried out using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Immunology 2022Quote: ... and Illumina linker addition to B cell heavy chain transcripts were performed using the human NEBNext Immune Sequencing Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... W165F and S169A) were introduced into human PSEN1 cDNA cloned into the pMSCVpuro vector using Q5 Site-Directed Mutagenesis Kit (NEB BioLabs) according to the standard protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... rRNA depletion was performed using a NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and RNA was purified using Agencourt RNAClean XP Beads (New England Biolabs). Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... was inserted into murine Shh between amino acids 92N and 93T (corresponding to N91 and T92 in human Shh) by using Gibson assembly (HiFi Assembly Kit, NEB). Where indicated ...
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bioRxiv - Molecular Biology 2024Quote: ... and the fourth copy of the human ubiquitin gene was cloned into the resulted backbone using NEBuilder HiFi DNA Assembly Cloning Kit (NEB). To generate the pmeDPC-56 substrate ...
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bioRxiv - Microbiology 2024Quote: ... whereas rRNA-depleted RNA was prepared from 1 μg of total RNA using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (New England Biolabs). NGS libraries were generated from poly(A)+ RNA or rRNA-depleted RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: ... were cloned N-terminally to human CISD1 and point mutations introduced by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis kit (NEB). All plasmids were sequence verified ...
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bioRxiv - Cell Biology 2024Quote: ... Bulk RNA sequencing of K562 cells at 37°C was conducted by generating a cDNA library prepared by depleting rRNA using the NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) and then the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) ...