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Citations for New England Biolabs :
251 - 300 of 10000+ citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... The reactions were then column-purified and we assembled the PCR amplified vector and promoters using Gibson assembly (Gibson et al., 2009) with NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). The assembly mix was then electroporated directly into the electrocompetent MG1655 strain ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Each reaction with randomly mutated promoter variants was column-purified before Gibson assembly with the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). Each assembly mix was then electroporated into the MG1655 strain and colonies that grew on LB with Kanamycin were picked for Sanger sequencing and stored as glycerol stocks ...
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bioRxiv - Developmental Biology 2020Quote: Each Mmp13 promoter sequence was amplified by PCR using Q5 Hot Start High-Fidelity DNA Polymerase (M0493L, NEB, Ipswich, MA, USA). To generate luciferase constructs ...
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bioRxiv - Molecular Biology 2021Quote: ... TIF3 alleles with the native promoter and terminator encoding C-terminal hexahistidine tagged-eIF4B or variants were Gibson-cloned (NEB, USA) into the BamHI site of single-copy vector pHLUM (21) ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmid was linearized with SacI or XhoI (for transcription from T7 or SP6 promoter respectively) and 3’UTR fragment was transcribed in vitro using SP6 or T7 polymerases (New England Biolabs, UK) and the DIG RNA labelling Mix (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... The SCR and the truncated versions Ei and Eii were cloned in front of the Sox2 promoter by linearizing the vector with AgeI (NEB, R3552) and subsequently using Gibson assembly cloning ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The mcr-1 gene along with its natural promoter was PCR-amplified from the natural PN16 (IncI2) plasmid using Q5® High-Fidelity DNA Polymerase (New England BioLabs). The amplified and purified mcr-1 fragment was assembled together with PCR-amplified pSEVA121 backbone using NEBuilder® HiFi DNA Assembly Master Mix according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... Reporter plasmids were assembled by Gibson Assembly using promoter PCR product and pHCKan-yibDp-GFPuv PCR product following manufacturer’s protocol (New England Biolabs, Ipswhich, MA). All plasmid sequences were confirmed by DNA sequencing (Genewiz ...
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bioRxiv - Biochemistry 2021Quote: ... were synthesized in the TCRB/TCRA orientation (Integrated DNA Technologies) and cloned into a lentiviral vector under the control of the pEF1α promoter using Gibson assembly (New England Biolabs Inc). For generation of TCRs ...
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bioRxiv - Microbiology 2022Quote: ... The mcr-1 gene along with its natural promoter was PCR-amplified from the natural PN16 (IncI2) plasmid using Q5® High-Fidelity DNA Polymerase (New England BioLabs). The amplified and purified mcr-1 fragment was cloned into PCR-amplified pSEVA121 backbone using NEBuilder® HiFi DNA Assembly Master Mix according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Both the insert and vector PCR reactions were then column-purified and we assembled the vector and promoter variants using Gibson assembly (Gibson et al. 2009) with NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). The assembly mix was then electroporated into the electrocompetent MG1655 strain ...
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bioRxiv - Genetics 2020Quote: ... In vitro transcription template was amplified with T7 promoter sequence containing primer and in vitro transcribed using T7 RNA polymerase (NEB, E2040S). The IVT RNAs were capped using Vaccinia virus capping enzyme (Cellscript ...
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bioRxiv - Genetics 2021Quote: ... We then recombined 500ng of this digestion (including ∼4.4kb of backbone vector and 250bp of filler sequence including a spliced region and truncated GFP ORF) with 150ng of both the purified enhancer and promoter products using Gibson assembly (NEB, E2611) for 1 hour at 50°C in a 40uL reaction and purified the reaction using 1X volume AMPure XP with 3 total ethanol washes.
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bioRxiv - Genomics 2021Quote: ... and cloned into Drosophila STARR-seq vectors containing either the DSCP or Rps12 core-promoters using Gibson cloning (New England BioLabs; E2611S). The oligo library for human STARR-seq screens was amplified (primers see Supplementary Table 1 ...
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bioRxiv - Microbiology 2020Quote: ... the full length of MoMca1 and MoMca2 genes including their native promoter were amplified and inserted into pBGt (40) fungal expression vector by using the Gibson Assembly approach (NEB BioLabs). The pBGt-MoMca1Mca2 expression vector was transformed into ΔMomca1mca2 protoplasts and selected on gentamicin-containing plates ...
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bioRxiv - Molecular Biology 2021Quote: ... A selection cassette carrying the Puromycin resistance gene driven by the PGK promoter and flanked by FRT sites was cloned in front of the Sox2 promoter by linearizing the piggyBac vector with the AsiSI (NEB, R0630) restriction enzyme ...
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bioRxiv - Genomics 2021Quote: ... Produced insert was cloned into the abovementioned pENTR vector from Twist Biosciences bearing GSH1 and GSH2 300bp homology arms as well as CMV promoter sequence using Gibson Assembly Master Mix (NEB, #E2611L). HDR donors were amplified from these plasmids using biotinylated primers with phosphorothioate bonds between the first 5 nucleotides on both 5’ and 3’ ends ...
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bioRxiv - Genomics 2022Quote: We first generated a transfer vector containing a reporter gene (hsp68 promoter + mScarlet) flanked by φC31 and BxbI attB sites using HiFi Assembly (NEB #E2621). The insulator sequences were obtained from their respective papers (cHS4 from Chung et al.9 ...
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bioRxiv - Genomics 2022Quote: ... The constructs were then inserted between the φC31 attB site and hsp68 promoter in the transfer vector by HiFi Assembly (NEB #E2621). The cHS4 mutant design is from Farrell et al.32 (cHS4 x3 ...
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bioRxiv - Plant Biology 2022Quote: ... the full region including the HrpL box promoter was PCR-amplified using primers (Supplementary Table S2) and Q5 High-Fidelity DNA Polymerase (NEB, USA). The resulting PCR fragment was gel-purified and was blunt-end-ligated into the Eco53kI (NEB ...
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bioRxiv - Genomics 2023Quote: ... Resulting sequences were cloned upstream of the core promoter (using KpnI restriction site) via Gibson assembly® (New England BioLabs, #E2611S). Each construct was verified using sanger sequencing and luciferase assays were performed as previously described1 ...
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bioRxiv - Plant Biology 2023Quote: The coding region of NIa-Pro protein was cloned from TuMV into the binary vector pMDC32 downstream of the 35S promoter as well in pSITE vector using Gibson assembly (New England Biolabs, USA)(Gibson et al. ...
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bioRxiv - Molecular Biology 2023Quote: We first amplified a 77 bp long minimal TK promoter followed by the mCherry coding sequence by PCR and inserted the fragment into an NcoI-HF®-digested (NEB) pGemT-vector (Promega) ...
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bioRxiv - Biophysics 2022Quote: ... were ordered from Integrated DNA Technologies (IDT), (Coralville, Iowa) as gBlock gene fragments downstream to a T7 promoter and flanked by EcoRI (New England Biolabs (NEB),Ipswich ...
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Enhancers display constrained sequence flexibility and context-specific modulation of motif functionbioRxiv - Genomics 2022Quote: ... Drosophila library fragments were amplified (primers see Supplementary Table S1) and cloned into Drosophila STARR-seq vectors containing the DSCP core-promoters using Gibson cloning (New England BioLabs; E2611S). The oligo library for human STARR-seq screens was amplified (primers see Supplementary Table S1 ...
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bioRxiv - Genomics 2022Quote: ... ERCC-00048 DNA with an upstream T7 promoter was cloned into pUC19 and PCR amplified (primers in Table S9) with Phusion polymerase (New England Biolabs M0530S) using the following cycling conditions ...
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bioRxiv - Plant Biology 2022Quote: ... The transformation vector pICU2:Cas9-dsRED containing the Cas9 gene from Streptococcus pyogenes expressed under the promoter of the Arabidopsis Incurvata 2 gene (ICU2, At5g67100) was combined with the gRNA combinations by Gibson assembly (NEB, USA). For RPB1 ...
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bioRxiv - Plant Biology 2022Quote: ... including the stop codon and (2) the CDS and native promoter region 1024 bp upstream using Phusion High Fidelity DNA polymerase (NEB, USA) and TA-ligated into the entry vector pCR8/GW/TOPO (Thermo ...
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bioRxiv - Developmental Biology 2023Quote: ... The 2-kpb cbln12 promoter (Dohaku et al., 2019) and lTl-Kaede-pAS were subcloned to pT2ALR-Dest by NEBuilder (NEB, USA). To generate Tg(5xUAS-hsp70l:HA-skor2-P2A-mCherry ...
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bioRxiv - Neuroscience 2024Quote: ... and Gas5 shRNAs (shRNA # 1= G5C1 and shRNA # 2= G5C2) were cloned by replacing the existing Luciferase shRNA (shRLuc) under a U6 promoter using BamHI (NEB #R0136L) and EcoRI restriction sites (Table 3) ...
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bioRxiv - Microbiology 2024Quote: ... The pBAD33 plasmid harboring the stem structure of rrsH (corresponding to -131∼-102 and +1559∼+1589 relative to +1 of rrsH gene) under T7 promoter was linearized by digestion with XbaI (NEB, R0145).
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bioRxiv - Systems Biology 2024Quote: ... These linearized plasmids with sticky ends were ligated to their corresponding ap1 promoter and lentiviral backbone pairs with T4 DNA ligase (NEB M0202S) and transformed into STBL3 competent cells (Invitrogen C737303 ...
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bioRxiv - Plant Biology 2024Quote: The plant transcriptional effector destination plasmid pMON-Gal4DBD-GW-mNeonGreen was made by amplifying the Gal4DBD-Gateway cassette fragment from pMpGWB102-GAL4DBD and introducing it into the PCR-amplified backbone of pMON999-mNeonGreen including the 35S promoter and mNeonGreen via NEBuilder® HiFi DNA assemblage (New England Biolabs). The Marchantia endogenous ARF expression destination plasmids pJL001 and pHKDW038 were made as previously described in Mutte et al ...
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bioRxiv - Plant Biology 2024Quote: ... Assembly reactions with the p2x35S (GB0222) promoter and pTnos terminator (GB0037) using BsaI and BsmBI (New England Biolabs, Ipswich, MA, USA) as restriction enzymes in 25-cycle digestion/ligation reactions were performed essentially as described by Sarrion-Perdigones et al ...
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bioRxiv - Systems Biology 2024Quote: ... and a customized lentiviral backbone with SFFV promoter and WPRE element were PCR amplified by Q5 high fidelity master mix (NEB, M0492S) and assembled by NEBuilder® HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Genetics 2024Quote: ... a DNA fragment containing a sgRNA scaffold and another U6 promoter was ligated to the BsmBI digested pgRNA library plasmids using T4 ligase (NEB, M0202M). The ligated products were electroporated into NEB 5-α electrocompetent Escherichia coli cells (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Placenta (M0307, New England Biolabs) and 25 U of M-MuLV Reverse Transcriptase with corresponding buffer (M0253 ...
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bioRxiv - Systems Biology 2024Quote: ... human (E6320, New England Biolabs, USA) and sequenced on a MiSeq (Illumina ...
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bioRxiv - Immunology 2021Quote: Point or deletion mutations were prepared from human or mouse CRT expression clones in pCMV3-C-Myc (SinoBiological) using the Q5® Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s protocol and primers designed using the NEBaseChanger™ web tool ...
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bioRxiv - Systems Biology 2020Quote: ... rRNA depletion and library preparation for sequencing was completed with the NEBNext protocol (‘Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Genetics 2020Quote: ... RNA-seq libraries were prepared according to the protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) ...
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bioRxiv - Molecular Biology 2023Quote: ... W165F and S169A) were introduced into human PSEN1 cDNA cloned into the pMSCVpuro vector using Q5 Site-Directed Mutagenesis Kit (NEB BioLabs) according to the standard protocol ...
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bioRxiv - Immunology 2023Quote: ... Ribosomal RNA from 1000 ng total extracted RNA was depleted using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat; New England Biolabs Inc.). The remaining RNA was used to produce the sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc. ...
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bioRxiv - Immunology 2024Quote: ... and Illumina linker addition to B cell heavy chain transcripts were performed using the human heavy chain only primers of NEBNext Immune Sequencing Kit (New England Biolabs #E6320) according to the manufacturer’s instructions (provided upon request) ...
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bioRxiv - Molecular Biology 2024Quote: The total RNA from the HG and LG treated βTC6 cells were rRNA depleted using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB), followed by fragmentation and the cDNA library preparation using the NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... ribosomal RNA (rRNA) depletion was performed using NEBNext rRNA depletion kit v2 (Human/Mouse/Rat) with RNA sample purification beads (NEB, #E7405X). cDNA libraries were constructed utilizing the NEBNext Ultra II Directional RNA Library Prep (stranded ...
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bioRxiv - Microbiology 2021Quote: ... The reporter was engineered to express the red fluorescent protein mKate2 from the tad/flp promoter by cloning this segment of DNA into plasmid pLL103 through digest by KpnI and SphI restriction enzymes (NEB, Ipswich, MA) to generate pJS2020.1.
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bioRxiv - Cell Biology 2022Quote: ... mTagBFP2 and Cas9-Avidin provided by Max Wilson into an expression vector bearing a CMV promoter and flanking PiggyBac transposase-compatible inverted terminal repeats using Gibson Assembly (New England BioLabs Inc., E2611L) according to supplier instructions ...
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bioRxiv - Physiology 2020Quote: ... pNP152 was obtained by insertion of the sta-2 promoter and sta-2 gene fused to Lifeact::mKate2 [75] into the MosSCI vector pCFJ151 [76] using Gibson Assembly (NEB Inc., MA) and confirmed by PCR or sequencing ...
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bioRxiv - Biochemistry 2021Quote: ... the linearized vector was assembled with a GBlock (Dataset S5) carrying the proD promoter (60) using HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, MA). Vectors used to construct plasmids containing the bbu genes were linearized by PCR amplification from 10 ng vector template DNA in a 50 μL reaction containing 0.5 μM of forward and reverse primers (Dataset S5 ...