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Citations for New England Biolabs :
101 - 150 of 10000+ citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... the PCR-amplified Cxcl1 promoter fragment was digested with KpnI and BglII (New England Biolabs) and then purified by gel extraction using the QIAquick Gel Extraction Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: ... We inserted it upstream of the TK- promoter into the SphI-HF®-digested (NEB) TK-mCherry plasmid ...
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bioRxiv - Molecular Biology 2024Quote: ... the Sox2-promoter-containing pCR Zero plasmid was digested with Xbal + SalI-HF (R3138S, NEB) and ligated (T4 Ligase 5 U/µL ...
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bioRxiv - Molecular Biology 2021Quote: ... depleted with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat, NEB, Figures 6 and S6), or not depleted (Figures 2B ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: DNA from human cells was isolated using the Monarch®Genomic DNA Purification Kit (NEB) following the manufacturer’s instructions and including the recommended RNaseA digestion step ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: RNA from human cells was isolated using the Monarch®Total RNA Miniprep Kit (NEB) following the manufacturer’s instructions and including the on-column DnaseI digestion ...
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bioRxiv - Neuroscience 2022Quote: ... was used to cut out Dlx enhancer and HBB promoter with MluI and EheI (NEB: R0606S).
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bioRxiv - Plant Biology 2021Quote: ... reilianum ortholog open reading frame or promoters were amplified by Phusion DNA polymerase (NEB, Ipswich, USA) with primers (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-fold molar access of pre-annealed T7 promoter adapters and either 80 units HindIII (NEB) or 40 units SpeI (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... The PBL35 and PBL36 promoters were introduced into a pCAMBIA1305,1 3xNLS-VENUS by Gibson cloning (NEB). The POL native promoter (1.6 kb-5’ ...
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bioRxiv - Plant Biology 2021Quote: ... and the Medicago truncatula MtU6.6 promoter and scaffold fragments from HindIII and EcoRI (New England BioLabs) digested pUC-gRNA shuttle vector (Jacobs et al. ...
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bioRxiv - Genetics 2023Quote: ... the 10XUAS-DSCP promoter was amplified from plasmid pNP with Q5 High-Fidelity DNA Polymerase (NEB) using primers UP0394 and UP0395 ...
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bioRxiv - Microbiology 2023Quote: ... and the fleQ gene and its native promoter were amplified using Phusion polymerase (New England BioLabs) with primers containing 20bp of homology to the mTn7/pMQ56 SmaI cut-site at their 5’ end ...
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bioRxiv - Developmental Biology 2023Quote: ... Complete sgDNA templates were assembled and amplified using the T7 promoter (Phusion High Fidelity, NEB E0553). sgRNA was then transcribed (MEGAshortscript T7 ...
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bioRxiv - Molecular Biology 2023Quote: ... downstream of a T7 promoter using overlap-extension PCR with Q5 High-Fidelity DNA Polymerase (NEB) and InFusion cloning (Takara Bio) ...
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bioRxiv - Bioengineering 2023Quote: ... Bricks were assembled into cassettes (promoter + ORF + terminator) via Golden Gate (using BsaI-HF v2.0 (NEB) and T4 DNA Ligase (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: The Gr64f promoter was PCR amplified using Q5 High-Fidelity 2× Master Mix (New England Biolabs) from the Gr64f-GAL4 (107 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were analysed by an MSD Elisa assay (Pacific Biolabs) using a CEP55 antibody (Novux ...
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bioRxiv - Molecular Biology 2024Quote: ... The PAGFP-TIRR plasmid was generated by amplifying a PAGFP fragment from a pPAGFP-C1 plasmid and a backbone containing TIRR using Q5® High-Fidelity DNA Polymerase and associated Q5® Reaction Buffer and GC enhancer (New England Biolabs). The PAGFP fragment was inserted C-terminally to TIRR.
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bioRxiv - Cancer Biology 2023Quote: ... library was prepared using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). The RNA-Seq libraries were sequenced on NextSeq 500 using the NextSeq 500/550 High Output Kit v2.5 (75 cycles ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was depleted using NEBNext® rRNA Depletion Kit (Bacteria) and NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) with RNA Sample Purification Beads (New England Biolabs, UK). An equimolar mixture of depletion solutions from each kit was used during the hybridization step to deplete both eukaryotic and prokaryotic rRNA simultaneously ...
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bioRxiv - Microbiology 2022Quote: ... Clean RNA was first rRNA depleted using the NEBNext rRNA depletion kit (Human/Mouse/Rat) (NEB) before being prepared for sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ribosomal RNA was removed using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) according to the manufacturer’s instructions with 6 µl total RNA used as input per sample ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the NEBNext rRNA Depletion Kit v2 (Human/ mouse) (NEB #E7405), NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Cell Biology 2024Quote: ... The mRNA was purified from human PAECs using Magnic mRNA isolation kit (New England Biolabs, S1550S). In order to obtain the required quantity of RNA ...
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bioRxiv - Microbiology 2021Quote: ... Chitinase genes and their endogenous promoters were amplified using Q5 High-Fidelity DNA Polymerase (New England Biolabs) with wild-type Salmonella IR715 as the template and the primer sets STM0233_Tn7Com_FW and STM0233_Tn7Com_RV or chiA_Tn7Com_FW and chiA_Tn7Com_RV ...
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bioRxiv - Genomics 2021Quote: ... the CMV enhancer/promoter region was amplified from the pcDNA3.0 backbone using Phusion High-Fidelity Polymerase (NEB) and subcloned into the pUC19 vector upstream of the circEfnb2 or circMini backsplicing cassettes ...
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bioRxiv - Plant Biology 2024Quote: ... after the CaMV35S promoter (p35S) by Gibson Assembly using NEBuilder® HiFi DNA Assembly Master Mix (NEB). The resulting plasmids containing p35S::CYP73A412-TurboID-EGFP ...
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bioRxiv - Plant Biology 2024Quote: ... the respective promoter was isolated via PCR using Phusion High Fidelity Enzyme (New England Biolabs, no. M0530L) and inserted upstream of GUS gene of pCambia1301 vectors ...
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bioRxiv - Microbiology 2022Quote: ... The glpFKD promoter fragments were labeled with 32P using DNA Polymerase I Large Fragment (Klenow fragment) (NEB) to fill in the 5’ overhang with [α-32P]dATP ...
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bioRxiv - Cancer Biology 2023Quote: ... The TERT promoter was then amplified with PCR using corresponding primers with the Q5 DNA polymerase (NEB) (Supplementary Table S1) ...
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bioRxiv - Neuroscience 2024Quote: ... was used to replace the CMV promoter in pAAV.U6.shRLuc.CMV.ZsGreen.SV40 (Penn Core Vector) plasmid by excising out the CMV promoter using the EcoRI (NEB #R3101) and NHE-I (NEB #R3131 ...
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bioRxiv - Biophysics 2024Quote: ... 25μg of the plasmid containing the hsp70 promoter sequence was digested with HindIII restriction enzyme (NEB R3104) for 3 hours at 37C ...
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bioRxiv - Biochemistry 2024Quote: Cy3-labeled enhancer-promoter DNAs used for condensation assay were generated by PCR using Taq polymerase (NEB) with a Cy3-labeled primer (IDT ...
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bioRxiv - Genomics 2024Quote: The 493 gRNAs with associated 10N random barcodes were ordered as an IDT oPool and PCR amplified with Q5 High-Fidelity polymerase (NEB, Cat. No. M0491S) to make double stranded DNA ...
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bioRxiv - Genomics 2024Quote: ... The PCR amplified IDT oPool gRNAs with associated 10N random barcodes were cloned into the linear backbone using NEBuilder HiFi DNA Assembly (NEB, Cat. No. E2621S) using 0.15 pmol of the insert (gRNA library ...
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bioRxiv - Molecular Biology 2022Quote: ... We then carried out rRNA depletion with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat NEB # E6310S) using the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and libraries were prepared using the NEBNext rRNA Depletion Kit for Human/Mouse/Rat (NEB Cat#E6310). Adapter-ligated cDNA was amplified with 11 cycles of PCR to generate libraries ∼300 bp in length ...
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bioRxiv - Developmental Biology 2022Quote: Complementary DNA (cDNA) encoding for Human CD36 (RC221976) was cloned using Hifi DNA assembly kit (NEB E5520S) into the pJFRC-MUH vector (Addgene ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was removed using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat; New England Biolabs), and the rRNA-depleted RNA was used to prepare libraries with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... All subsequent HI.fate vectors containing different internal promoters were constructed by digesting HI.fate.E with BsiW I and Sac II restriction enzymes (NEB) and cloning each promoter ...
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bioRxiv - Microbiology 2021Quote: ... Promoter DNA fragments were excised from plasmid pSR and end-labelled with γ32-ATP using T4 PNK (NEB). EMSAs and DNase I footprints were done as previously described (42) ...
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bioRxiv - Immunology 2021Quote: ... The purified Cxcl1 promoter fragment was equally split into two groups: one treated with M.SssI (New England Biolabs) and the other without M.SssI (“mock” ...
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bioRxiv - Molecular Biology 2022Quote: Bclx and CD44-v5 minigenes were cloned in EGFP-C1 plasmid following the CMV promoter using ExoIII (NEB) enzyme following manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Wildtype apaH or yjeP expressed from the constitutive promoter J23119 (http://parts.igem.org/Part:BBa_J23119) were cloned into XhoI and HindIII (New England Biolabs, MA, USA) digested pZS21 ...
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bioRxiv - Molecular Biology 2021Quote: ... Promoters (Supplemental Figure 1, Supplemental Table 3) were amplified from genomic DNA using Q5 polymerase (New England Biolabs) and inserted between the enhancer and 24xMS2 sequences using restriction enzyme-mediated ligation ...
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bioRxiv - Microbiology 2023Quote: Promoter DNA fragments were excised from plasmid pSR and end-labelled with γ32-ATP using T4 PNK (NEB). EMSAs and DNase I footprints were done as previously described 40 ...
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bioRxiv - Plant Biology 2023Quote: ... Promoters and coding sequences were commercially synthesized (Eurofins) or PCR-amplified using the Q5 high fidelity polymerase (NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... These constructs were placed under the control of the T7 promoter and inserted into EcoRI (New England Biolabs) and NotI (New England Biolabs ...
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bioRxiv - Bioengineering 2022Quote: ... were introduced through Gibson Assembly between a T7 promoter and T7 terminator in a pUC19-based vector (NEB), pMZL ...