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Citations for New England Biolabs :
201 - 250 of 2243 citations for Estriol 2 4 16 17 D4 98% 95% Pure since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: The library was ligated to the barcode oligonucleotide at a 7:1 oligo:library ratio overnight at 16 °C with T4 DNA ligase (New England Biolabs). A no-insert negative control was also ligated overnight using identical conditions ...
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bioRxiv - Microbiology 2023Quote: ... Near full-length 16S rRNA genes were amplified by PCR using Phusion Hi-Fidelity DNA Polymerase (New England BioLabs) and a bacteria-specific primer set ...
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bioRxiv - Molecular Biology 2023Quote: ... a ratio of 7:1 (oligo:library) was used overnight at 16 °C with T4 DNA ligase (New England Biolabs). The products were purified and eluted in 6 μL water (Zymo Clean and Concentrate) ...
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bioRxiv - Cell Biology 2023Quote: ... The ligation (400 μl) was performed overnight at 16°C in 1x T4 Ligase buffer (New England Biolabs (NEB)) supplemented with 5 mM Mg(OAc)2 ...
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bioRxiv - Cancer Biology 2023Quote: ... 16 reactions were carried out per amplicon per cell line using Q5 High-Fidelity 2x master mix (NEB, #M0492L) with primers containing partial Illumina adapters ...
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bioRxiv - Molecular Biology 2023Quote: ... T7 transcription was performed for 16 h and then RNA was purified using the Monarch RNA Cleanup Kit (NEB). In vitro cleavage was performed with purified recombinant ...
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bioRxiv - Cell Biology 2023Quote: ... The ligation (400 μl) was performed overnight at 16°C in 1x T4 Ligase buffer (New England Biolabs (NEB)) supplemented with 5 mM Mg(OAc)2 ...
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bioRxiv - Neuroscience 2021Quote: ... 2 (NEB #E7500) and 3 (NEB #E7710 ...
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bioRxiv - Cancer Biology 2020Quote: ... 4 μl of RCA mixture was combined with 4 μl Restriction enzyme buffer (New England Biolabs), 13 μl MilliQ ...
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bioRxiv - Molecular Biology 2019Quote: ... and 4 U of SssI (NEB) or 4 µl purified Eco72IM (diluted 1:100 ...
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bioRxiv - Neuroscience 2019Quote: ... in 1X NEBbuffer 4 (NEB #B7004), or an equivalent volume of ultra-pure water and 1X NEBbuffer 4 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 4 U DNase I (NEB) was used for each 200 μl to digest the DNA nanoswitches at 37 °C for 1 hour ...
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bioRxiv - Bioengineering 2021Quote: ... 4 μl 10mM dNTPs (N0477L, NEB), 1 μl RNase Inhibitor (30281 ...
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bioRxiv - Genetics 2020Quote: ... 4 μl T4 DNA polymerase (NEB), 1 μl DNA polymerase Klenow (NEB) ...
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bioRxiv - Genomics 2019Quote: ... 4 μl Klenow fragment (NEB M0212L) and 10 μl 10 nM dATP 20 min at 37 °C and 20 min at 75°C to inactivate the reaction ...
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bioRxiv - Physiology 2019Quote: ... 4 μl of 10xG7 Buffer (NEB), 4 μl of 10% NP-40 (NEB ...
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bioRxiv - Pathology 2019Quote: ... 4 U/ml RNase inhibitor (NEB) and proteinase inhibitor cocktail (Roche ...
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bioRxiv - Biophysics 2019Quote: ... 4 µL Murine RNase inhibitor (NEB), 1 µL T7 polymerase (NEB) ...
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bioRxiv - Pathology 2020Quote: ... 4 U/ml RNase inhibitor (NEB) and proteinase inhibitor cocktail (Roche ...
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bioRxiv - Genetics 2020Quote: ... 4 Units of terminal transferase (NEB) were added together with dCTP in a final concentration of 0.1 mM ...
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bioRxiv - Biochemistry 2021Quote: ... 4 U of Proteinase K (NEB) were added to the reaction and incubation continued for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... 4 mg/ml BSA (NEB B9000S), 300 U/μl RL Lysozyme ...
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bioRxiv - Microbiology 2020Quote: ... and 4 U/ml apyrase (NEB) were added and the reaction was incubated overnight at room temperature ...
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bioRxiv - Genomics 2023Quote: ... 3 µL 10x NEBuffer 4 (NEB), 3 µL of 1 mM each dATP/ddCTP/ddGTP/ddTTP (dATP/ddBTP ...
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bioRxiv - Genomics 2024Quote: ... 4 μl of Inorganic Pyrophosphatase (NEB) and 2 μl of Phi29 DNA Polymerase (NEB) ...
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bioRxiv - Genomics 2024Quote: ... 0.67x NEB buffer 4 (NEB, B7004S), 0.13% Triton X-100 (sigma ...
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bioRxiv - Systems Biology 2019Quote: ... vaccinia mRNA 2’-O-methyltransferase (NEB, 250 U every 2 h) and water ...
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bioRxiv - Molecular Biology 2023Quote: ... and 2′ O-methylated using Vaccinia VP39 (2′ O Methyltransferase) (NEB) in a reaction that also included 1X capping buffer (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were individually treated for 16 h at 37 °C with 20 units of DpnI enzyme (New England Biolabs). Following gel extraction using Wizard SV Gel and PCR CleanUp System (Promega) ...
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bioRxiv - Biophysics 2022Quote: ... was performed using plasmid pHSCRP-His6-H17C-C178S [constructed from plasmid pAKCRP-His6 (16) using site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB)].
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bioRxiv - Genomics 2019Quote: ... 400 ng of purified DNA was ligated overnight at 16 °C to adapters (Table S4) with T4 DNA ligase (NEB). Ligated DNA was purified using MiniElute columns (Qiagen) ...
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bioRxiv - Neuroscience 2019Quote: ... pRSET-dNOS exon 16 DNA was then transformed into T7 Express lysY/lq E.coli protein expression cells (New England Biolabs #C3013) and plated on LB plus ampicillin (60 µl/ml) ...
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bioRxiv - Genomics 2020Quote: ... at 37°C overnight and purified digests were ligated overnight at 16°C using T7 DNA ligase as per manufacturer’s instructions (NEB M0318). Ligation reactions were transformed into One Shot™ Stbl3™ chemically-competent E.coli cells (42°C heat-shock ...
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bioRxiv - Molecular Biology 2019Quote: Different CpG-containing oligonucleotides with 5’-TTAA overhangs were annealed and end-to-end ligated overnight at 16°C with T4 ligase (NEB). Oligos were ethanol-precipitated and filled-in with 1 mM biotinylated dUTP (Thermo Fisher ...
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bioRxiv - Microbiology 2019Quote: ... followed by size exclusion chromatography (HiLoad 16/60 Superdex 200) using the following buffers: lysis buffer (wash buffer supplemented with protease inhibitor, DNase I [NEB]), wash buffer (20mM HEPES-KOH pH 8.0 ...
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bioRxiv - Biophysics 2019Quote: ... Both the 2 kb spacer and 1.5 kb biotin handle DNA were ligated to 5’-CGGT 1 µm polystyrene oligo beads overnight at 16 °C using T4 DNA ligase (NEB). The ligated beads were first washed with TE + 0.5 M KCl + 20 µg/mL β-casein ...
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bioRxiv - Neuroscience 2020Quote: ... either purified directly (insert) or from agarose gel (vector) and ligated (16-18 h, 20-22 °C) with T4 DNA ligase (NEB) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2021Quote: ... using auto-induction protocols (Studier, 2005) at 16 °C for 20 h or in BL21 pLysY (New England Biolabs, C3010I) for isotopically labelled proteins with IPTG induction for 16 h at 20 °C ...
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bioRxiv - Microbiology 2020Quote: RNA samples were confirmed to be DNA-free by conducting test PCRs on the 16S rRNA gene and converted to cDNA using the LunaScript RT SuperMix Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Regions of 16S rDNA gene were amplified by PCR from extracted DNA with the Q5 high-fidelity DNA polymerase (New England BioLabs) using the universal primers 926F ...
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bioRxiv - Cancer Biology 2022Quote: ... were then ligated overnight at 16°C with the dA-tailed DNA fragments in the presence of 800 units of T4 DNA ligase (NEB) and 1 mM ATP (Roche ...
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bioRxiv - Microbiology 2021Quote: ... Ligation of gRNA sequence into the plasmid was made using a T4 DNA ligase at 16°C overnight and transformed into DH5α chemically competent bacteria according to the manufacturer protocol (NEB). The presence of the new gRNA into pCas9-amdSYM was confirmed by Sanger sequencing using the M13 forward primer ...
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bioRxiv - Microbiology 2021Quote: ... The V1-V2 region of the 16S rRNA genes was amplified with Q5 High-Fidelity Polymerase Kit (New England Biolabs). (F’:AATGATACGGCGACCACCGAGATCTACAC-TATGGTAATT-CC-AGMGTTYGATYMTGGCTCAG ...
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bioRxiv - Microbiology 2022Quote: ... gDNA fragments were ligated with annealed adaptors overnight at 16 °C in the presence of T4 DNA ligase (NEB#M0202). Bands from 200 to 400 bp were selected by extraction from a 2% agarose gel using a Monarch DNA Gel Extraction Kit (NEB#T1020L) ...
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bioRxiv - Cancer Biology 2021Quote: ... chromatin from both IP and ‘Input’ samples were eluted and de-crosslinked at 65°C for 16 hours followed by DNA isolation by PCR and DNA cleanup kit (NEB). Enrichment of AR and MED1 at specified enhancers were quantified by qPCR using specific primers ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mM CaCl2 was added and the His8 tag was cleaved through the addition of 8-16 U/mL enterokinase (New England Biolabs) for 2 days at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... Tubes were centrifuged at 8000 xg for 30 sec and supernatants were used for 16S rRNA gene PCR amplification with Phusion High-Fidelity DNA Polymerase (New England Biolabs) in a 20 μL reaction according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 16 μl of SSS reaction products were combined with 24 μl HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) reagent mix containing 4 μl T7 Buffer ...
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bioRxiv - Plant Biology 2022Quote: ... PCR was performed with primers 515F–806R targeting the V4 region of the 16S rRNA using Phusion High-Fidelity DNA Polymerase (NEB). The PCR product concentration was measured with Quant-iT PicoGreen dsDNA Assay Kits (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were collected by centrifugation and in situ ligation was performed overnight at 16°C in 500 μL volumes of 1X ligase buffer (NEB) with 20,000 U T4 DNA ligase ...