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Citations for New England Biolabs :
251 - 300 of 2243 citations for Estriol 2 4 16 17 D4 98% 95% Pure since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and the eluates were treated with proteinase K for 16 h at 65°C before library synthesis using NEBNext Ultra II DNA library preparation kits (New England Biolabs). The samples were sequenced on HiSeq 2000 (Illumina) ...
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bioRxiv - Biochemistry 2023Quote: ... and assembled using T4-ligase and BsaI-HF-v2 using cycles of 16°C and 37°C (New-England Biolabs). The resulting assembly is PCR amplified to add the final gates and assembled into a pBAD vector with appropriate gates.
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bioRxiv - Genomics 2023Quote: ... Samples were diluted in 8 ml of T4 DNA ligase buffer 1X and incubated 8 hours at 16°C with 8000 Units of T4 DNA ligase (NEB). Crosslinks were reversed overnight at 60°C in the presence of proteinase K (0.125 mg / ml final ...
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bioRxiv - Molecular Biology 2023Quote: ... The variant library was ligated into each vector with a 1:3 (insert: vector) T4 ligation at 16°C overnight (NEB). Ligations were DNA cleaned (Zymo) ...
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bioRxiv - Microbiology 2023Quote: ... Purified inserts and pTM267 backbone were then ligated together using overnight ligation at 16 °C with T4 DNA ligase (NEB). Ligation products were then transformed into chemically competent E ...
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bioRxiv - Immunology 2023Quote: ... Unincorporated primers were digested from the pre-amplified samples using 16 U/μl Exonuclease I (E. coli, New England Biolabs) at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The insert pool and digested backbone were then ligated overnight at 16°C using T4 DNA Ligase (New England Biolabs) with a vector-to-insert ratio of 1:1 ...
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bioRxiv - Biophysics 2023Quote: ... Assessment of protein phase separation or aggregation was performed by applying a total volume of 20 µL of the sample to a pretreated (Gallego et al., 2020) 16-well glass-bottom ChamberSLIP slide (Grace, BioLabs). DIC imaging was performed as previously described (Gallego et al. ...
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bioRxiv - Plant Biology 2023Quote: ... The fully reassembled BdPOX gene was ligated overnight at 16°C with the previously digested (FastDigest Eco31I) and dephosphorylated pGGC000 entry vector (with Antarctic Phosphatase, NEB) to generate pGGC_BdPOX ...
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bioRxiv - Microbiology 2023Quote: ... the full-length 16S rDNA sequence amplified with primers 8F and 1522R (Turner et al., 1999) with High Fidelity Q5 Taq polymerase (NEB). A high-quality sequence was obtained by the Sanger method and was used to query the 16S rRNA BLAST database ...
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bioRxiv - Microbiology 2023Quote: ... using JLHP836/838 and cloned into BamHI and NheI digested pDSW1278 (15) by Gibson assembly (16) using NEBuilder HiFi DNA Assembly Master Mix (NEB). This plasmid was conjugated into C ...
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Broad variation in response of individual introns to splicing inhibitors in a humanized yeast strainbioRxiv - Molecular Biology 2023Quote: ... This plasmid was cut with BaeI and a guide sequence DNA targeting the genomic region encoding Hsh155 HRs 15-16 region (Table S1) was inserted using HiFi DNA Assembly (New England BioLabs) to make p416-TEF1p-Cas9-NLS-crRNA-HSH155.
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplification was performed using NEB primers for 15-16 cycles using the Q5 Hot Start HiFi PCR Master Mix (NEB). The PCR-amplified library was purified using Ampure XP beads and its quality was assessed on a Bioanalyzer 2100 system (Agilent) ...
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bioRxiv - Genomics 2024Quote: ... A 7:1 molar ratio of barcode oligonucleotide to sublibrary was ligated overnight at 16°C with T4 DNA ligase (New England Biolabs).
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bioRxiv - Neuroscience 2024Quote: ... The final cDNA sample was then split into 16 separate reactions for final PCR amplification using 16 unique Illumina indexing primers and Q5 Hot Start High-Fidelity 2X Master Mix (NEB). After amplifications ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were diluted in 8 ml of T4 DNA ligase buffer 1X and incubated 8 hours at 16°C with 8000 Units of T4 DNA ligase (NEB). Crosslinks were reversed overnight at 60°C in the presence of proteinase K (0.125 mg / ml final ...
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bioRxiv - Molecular Biology 2019Quote: ... and 2 μl of DNase I (2 U/μl, New England Biolabs) to 30 μl RNA product ...
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bioRxiv - Biochemistry 2023Quote: ... and 2′O-methylated using Vaccinia 2′O Methyltransferase (New England Biolabs). The IRES-containing mRNAs were uncapped and polyadenylated.
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bioRxiv - Developmental Biology 2023Quote: Rehydrated embryos were blocked for 2 hs in 2% BSA (B9000, NEB) in PBS with 0.3% Triton X-100 (T9284 Sigma) ...
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bioRxiv - Genomics 2021Quote: ... 2 U M.CviPI (NEB), 160 μM S-adenosylmethionine (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mM dNTPs (NEB), 0.5 µM forward and reverse primers (IDT) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 2 μl DTT (NEB) and 2 μl of ProtoScript® II were added and the mixture incubated at 42°C for 12 hours with a 105°C heated lid ...
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bioRxiv - Microbiology 2023Quote: ... in NEBuffer 2 (NEB) for 2 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM ATP (NEB) in a total volume of 25 μl ...
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bioRxiv - Genomics 2023Quote: ... 1X NEBuffer 2 (NEB), 0.5 μM P5 primer ...
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bioRxiv - Bioengineering 2021Quote: ... 4 µg plasmid was digested for 4 h at 37°C with BstXI and XhoI (New England Biolabs). Products were run on a 1% agarose gel for 30 min at 120 V ...
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bioRxiv - Cancer Biology 2019Quote: ... Paired end strand-specific whole transcriptome libraries were prepared for 16 pairs using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, UK), following rRNA depletion with NEBNext rRNA Depletion Kit or Ribo-Zero Gold ...
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bioRxiv - Biochemistry 2020Quote: ... The dried glycoproteins were incubated with trypsin for 16 h at 37°C before releasing N-glycans using PNGase F (New England Biolabs, MA). Liberated N-glycans were separated from glycopeptides by C18 Sep Pak SPE cartridges (Waters Corporation ...
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bioRxiv - Microbiology 2022Quote: ... The following day swab samples were vortexed and spun down and 200 ul of the swab samples medium was combined with 16 ug/ml working concentration of TPCK-treated trypsin (New England Biolabs Ltd), 2x A/A/p/s antifungal/antibiotic solution (Wisent) ...
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bioRxiv - Zoology 2020Quote: The 16S rRNA and gltA amplicons from PCR assays were cleaned with the ExoSAP-IT kit (E1050, New England Biolabs, US) and Sanger sequenced ...
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bioRxiv - Cell Biology 2021Quote: ... HindIII and BamHI restriction sites were inserted just after codon coding for Proline 16 using the Q5-insertion mutagenesis (NEB, #E0554) according to the protocol from the manufacturer ...
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bioRxiv - Systems Biology 2023Quote: ... The first round was performed with primer pairs listed in Supplementary Table S7 with 16 cycles using NEBNext High Fidelity Master Mix (NEB, # M0541) for the screens with EpiC and Tiling libraries and NEBNext Ultra II Q5 Master Mix (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The unique barcoded region was amplified from 500ng genomic DNA with 16 cycles of PCR using NEBNext Ultra II Q5 master mix (NEB M0544L) as described in30 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We prepared genomic DNA libraries for the 16 samples using NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, Inc). We quantified the quality of the genomic DNA library using the Agilent 2100 Bioanalyzer system located at the Genome Analysis Core Laboratories at Georgia Institute of Technology (Agilent Technologies ...
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bioRxiv - Genomics 2022Quote: ... We then ordered an oligo containing 16 Ns with flanking homology arms to the landing pad plasmid (GWLP P1) and used HiFi Assembly (NEB #E2621) to assemble the oligo to the plasmid (50℃ ...
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bioRxiv - Microbiology 2023Quote: ... Two μl of a 1:250 dilution of the annealed oligos were ligated to 50 ng of dephosphorylated BsaI-digested vector at 16°C overnight in 20 μl final volume containing 1x T4 DNA ligation buffer (NEB, B0202S), and 400U T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 U of T4 DNA polymerase (NEB), 13.5 U of T4 Polynucleotide Kinase (NEB ...
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bioRxiv - Physiology 2019Quote: ... 4 μl of 10% NP-40 (NEB) and 8 μl of PNGase F (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 µL RNase H (New England Biolabs), 644 µL buffer A and 2 µL DTT (1M) ...
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bioRxiv - Biochemistry 2021Quote: ... and 4 U murine RNase inhibitor (NEB). TMAO was adjusted to pH 7.5 in a 6 M stock solution with HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... and 4 µL 10X CutSmart Buffer (NEB) in a 40 µL reaction ...
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bioRxiv - Synthetic Biology 2022Quote: ... 4 μL of Quick CIP (NEB, M0508S) was spiked into the reaction and incubated at 37°C for 30 minutes to dephosphorylate unincorporated dNTPs that may inhibit downstream processes ...
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bioRxiv - Synthetic Biology 2022Quote: ... 4 μL of T5 Exonuclease (NEB M0663S) was added to the reaction and incubated at 37°C for 30 minutes to remove unassembled products ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1 μl 10X NEB 4 buffer (NEB), 0.1 μl 10 mM dCTP and H2O ...
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bioRxiv - Biophysics 2022Quote: ○ 4 μL Klenow Exo-enzyme (NEB #M0212S) μL
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bioRxiv - Microbiology 2024Quote: ... 4 µl of 10x Apyrase buffer (NEB) and 1 µl of Apyrase (M0398S ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of 10x r3.1 buffer (NEB), 2 µl of 100µM DTT and 1 µl of NudC (M0607S ...
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bioRxiv - Genetics 2020Quote: ... followed by gap repair by adding 2 µl of 10× NEBuffer 2 (NEB), 3 µl of dNTPs (2.5 mM each ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2’O-methylated using Vaccinia VP39 (2’O Methyltransferase) (New England Biolabs), then purified by phenol-chloroform extraction and ethanol precipitation.
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bioRxiv - Genetics 2021Quote: ... Supernatant containing genomic DNA was used as a template for the PCR amplification of the 16S rRNA gene using Taq polymerase (New England Biolabs, Ipswich, MA) and the primers oMC98f (5’-GAAGAGTTTGATCATGGCTC-3’ ...