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Citations for New England Biolabs :
1 - 50 of 2243 citations for Estriol 2 4 16 17 D4 98% 95% Pure since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... primers that contained restriction sites for BamHI and XmaI or XbaI (Table S5, primers No. 9-16 and 95-98) and the high-fidelity polymerase Q5 (New England Biolabs) according to vendor’s manual ...
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bioRxiv - Molecular Biology 2023Quote: ... 16 U/µl of T4 RNA ligase 2 truncated KQ (NEB), 10°C 20 h ...
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bioRxiv - Synthetic Biology 2021Quote: ... annealed by temperature decrease from 95 °C to 4 °C and phosphorylated using T4 Polynucleotide Kinase (NEB, M0201) according to manufactures’ protocol ...
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bioRxiv - Genomics 2022Quote: ... 16 µl RNA were mixed with 4 µl LunaScript master mix (NEB cat# E3010L), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... We used PURE system (NEB), wheat germ extract (Promega) ...
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bioRxiv - Biophysics 2024Quote: ... A 4°C AKTA Pure FPLC system (Cytiva) was prepared with a 10 mL amylose column (NEB #E8021S) and 2 mL/min flow rate ...
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bioRxiv - Microbiology 2019Quote: ... and 4 μL was ligated at 16°C overnight with the T4 DNA ligase (NEB M0202S). After transformation into chemically-competent Top10 E ...
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bioRxiv - Biochemistry 2021Quote: ... 95 nmol of streptavidin (New England Biolabs) was added to each sample ...
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bioRxiv - Genomics 2022Quote: ... Pure PhiX174 ssDNA was purchased from NEB. Phage dsDNA concentration was estimated using a Qubit device ...
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bioRxiv - Genomics 2023Quote: ... Ligation was performed for 4 h at 16°C using 10,000 units of T4 DNA ligase (NEB) in 1.2 mL of ligation buffer (120 μL of 10× T4 DNA ligase buffer ...
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bioRxiv - Microbiology 2023Quote: ... followed by 12 cycles of 95°C for 15 seconds and 60°C for 4 min followed by exonuclease I (New England Biolabs, PN M0293S) treatment at 37°C for 30 minutes and 80°C for 15 minutes to remove any unincorporated primers ...
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bioRxiv - Plant Biology 2020Quote: ... 17 μl of the PCR reaction were mixed with 2 μl of CutSmart Buffer and 1 μl of AvaI (NEB) for a final volume of 20 μl ...
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bioRxiv - Molecular Biology 2019Quote: ... As a positive control, a concentration range (0.25, 1, 4, 16 units) of Dam enzyme was used (New England BioLabs #M0222S). Next ...
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bioRxiv - Plant Biology 2021Quote: ... DNA was ligated by incubation at 16°C for 5h in 4 ml volume using 100U of T4 DNA ligase (NEB). After reverse crosslinking and Proteinase K treatment (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... 2 μL of 10x buffer 4 (New England Biolabs, NEB), 2 μL of 1 mg/ml bovine serum albumin solution (NEB) ...
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bioRxiv - Genomics 2021Quote: ... 2 μL of 10x buffer 4 (New England Biolabs, NEB), 2 μL of 1 mg/ml bovine serum albumin solution (NEB) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 4 μl T4 RNA ligase 2 (NEB, catalog no. M0239) for 16 hours at 16°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL ligation mix (2 µL 10x T4 ligase buffer, NEB, 1 µL T4 RNA ligase 2 ...
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bioRxiv - Microbiology 2021Quote: ... 16 nM λ-DNA (NEB #N3011S) in 0.120 M NaCl was heated to 80°C for 10 minutes followed by 5 minutes on ice ...
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bioRxiv - Plant Biology 2021Quote: ... The cloning reaction consists of 25 cycles of 3 min at 37°C for digestion and 4 min at 16°C for ligation combining the restriction enzymes BsaI-HF (New England BioLabs, Ipswich, UK) for level 1 reaction or BpiI (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were incubated at 16 °C for 4 h in the presence of 1.15x of T4 ligation buffer (New England Biolabs, catalog no. B0202S) and 100U T4 ligase ...
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bioRxiv - Microbiology 2023Quote: ... DNA was ligated for 4 h in a water bath at 16 °C (30 Weiss Units of T4 DNA Ligase, M0202 New England BioLabs® Inc). 300 μg of Proteinase K was used to reverse the cross-linking overnight at 65°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM MgCl2 and 4 units of RNase Inhibitor Murine (NEB, M0314). After 30 min at room temperature ...
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bioRxiv - Genomics 2019Quote: ... 2 µg of DNA were digested for 4 hours with MmeI (NEB) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The amplification of bacterial DNA was achieved by targeting the V3–V4 region of 16S rRNA gene with 30 µL final volume containing 15 µL of 2× master mix (BioLabs, USA), 3 µL of template DNA ...
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bioRxiv - Microbiology 2023Quote: ... and 5 μM dNTP in 17 μL 1x rCutSmart buffer (NEB). The RNA and primers were then annealed by incubating as follows ...
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bioRxiv - Cell Biology 2023Quote: ... BS3-d0/BS3-d4 ((bis(sulfosuccinimidyl)suberate-d0/d4, Thermoscientific, Ref. 21590 and 21595), DSBU (Disuccinimidyl Dibutyric Urea, Thermoscientific, Ref. A35459, SNAP-Cell® Block (New England Biolabs Ref. S9106S), glutaraldehyde ...
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bioRxiv - Biophysics 2019Quote: ... λ-DNA (16 nM, New England Biolabs) was first heated in presence of 120 mM Na+ at 80 oC for 10 min and then quenched on ice for 5 min ...
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bioRxiv - Bioengineering 2021Quote: ... 16 U/mL Proteinase K (P8107S, NEB). The tissues were incubated at 37 °C for 40 min.
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bioRxiv - Bioengineering 2021Quote: ... 16 U/mL Proteinase K (P8107S, NEB)) ...
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bioRxiv - Bioengineering 2020Quote: ... with supplements (16 U RNase inhibitor (NEB), 5 mM FMN) ...
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bioRxiv - Systems Biology 2023Quote: ... 16 mU/uL proteinase K (NEB, P8107S)) ...
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bioRxiv - Physiology 2024Quote: ... 16 U/mL Proteinase K (P8107S, NEB)) at 37 °C for 40 min ...
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bioRxiv - Immunology 2023Quote: ... The libraries were generated by blunt-end cloning of 1,200 ng fragmented DNA into 1,000 ng PmeI linearized and dephosphorylated pHORF3 library vector (a gift from Michael Hust, Technische Universität Braunschweig) (16 hr at 16 °C, T4 DNA Ligase, NEB). The ligation reaction was purified and transformed into TG1 bacteria (Lucigen ...
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bioRxiv - Genomics 2019Quote: ... and resuspended in 95 µL PNK solution (1× T4 DNA ligase buffer (NEB), 0.1% Triton X-100) ...
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bioRxiv - Developmental Biology 2021Quote: ... and 16 μl of proteinase K (NEB, P8107S) was added to each polysome fraction ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed four times with 2×SSC and stored at 4 ⁰C in 2×SSC supplemented with 1:1000 murine RNase inhibitor (NEB M0314L) prior to imaging.
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bioRxiv - Plant Biology 2022Quote: ... The library was amplified 17 cycles by Q5 high fidelity polymerase (NEB, M0491L), and purified by AMPure XP beads (Beckman ...
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bioRxiv - Plant Biology 2023Quote: ... The library was amplified 17 cycles by Q5 high fidelity polymerase (NEB, M0491L). Antibodies used for histone modifications are the same as previous reported (Zhao et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... was digested for 16 hours with BsaI-HFv2 (NEB), after which the 2.2 kb fragment was isolated from gel ...
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bioRxiv - Synthetic Biology 2019Quote: ... 5 μl of the resulting amplicon were diluted 1:4 in 1x buffer 2 (NEB), followed by denaturation and re-annealing in a nexus GSX1 Mastercycler (Eppendorf ...
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bioRxiv - Molecular Biology 2023Quote: RNA was denatured for 5 min at 95°C in 1x RNA Loading Dye (NEB) and separated on denaturing polyacrylamide gels (12% ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL ligation mix (2 µL 10x T4 ligase buffer, NEB, 1 µL T4 RNA ligase 2, truncated, NEB, 1 µL Ribolock inhibitor) was added and incubated (1 h ...
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bioRxiv - Biochemistry 2021Quote: ... Pure fractions were mixed with 500 units Quick Calf Intestinal Alkaline Phosphatase (New England BioLabs) per 1 mg SMARCAD1 for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... The PCR products (∼300 ng) were incubated with 2 μl 10X NEBuffer 4 (New England Biolabs), 1 μl BtsCI restriction enzyme ...
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bioRxiv - Molecular Biology 2023Quote: ... and 16 μl of 10× rCutSmart buffer (NEB, no. B6004S) for 30 min at 37 °C ...
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bioRxiv - Plant Biology 2024Quote: ... and ligated overnight at 16°C (T4 DNA ligase; NEB). The same approach was used for the second deletion (353 bp ...
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bioRxiv - Biochemistry 2023Quote: ... 17 μl of the lysates was treated with 500 U PNGase F (NEB, cat# P0704S), 500 U Endo H (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Extracts were diluted to 1μg pure nucleotide concentration and purified by DNase treatment (New England Biolabs). cDNA was synthesized from RNA by using the iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... diluted in pure water and quantified with the Luna® Universal qPCR Master mix from NEB as previously described.