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Citations for New England Biolabs :
101 - 150 of 2243 citations for Estriol 2 4 16 17 D4 98% 95% Pure since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: We selected 17 sSNVs identified in brain-active enhancers and attempted cloning and site-directed mutagenesis (New England Biolabs) to recreate the mutations ...
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bioRxiv - Developmental Biology 2023Quote: RNA was isolated from 17 hpf Ciona robusta larvae by Monarch Total RNA miniprep kit (New England BioLabs Inc.). cDNA was produced by Omniscript reverse transcription kit (QIAGEN) ...
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bioRxiv - Immunology 2023Quote: pals-17::gfp and pals-20::wrmScarlet translational reporter constructs were assembled using NEBuilder HiFi kit (New England Biolabs) by fusing PCR products and gene fragments ...
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bioRxiv - Molecular Biology 2021Quote: ... Ligation was conducted overnight at 16 °C with T4 DNA Ligase (New England Biolabs). All minigene mutations were introduced via Q5 Site-Directed Mutagenesis Kit (New England Biolabs) ...
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bioRxiv - Genomics 2020Quote: ... with BspQ1 overhangs were ligated using T7 DNA ligase (NEB M0318; 16°C overnight) into BspQ1 digested (NEB R0712 ...
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bioRxiv - Molecular Biology 2022Quote: ... and ligated at 16 °C with 10,000 U of T4 DNA ligase (NEB, M0202) in ligase buffer supplemented with 0.8% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... The amplified backbone was treated with Dpn1 for 16 hours at 37°C (NEB) followed by a purification using a spin column ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were additionally treated with 16 units of PNGase F (New England Biolabs) per μL of lysate complemented with 1% NP-40 ...
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bioRxiv - Biophysics 2023Quote: ... the buffer was supplemented with 16 mM ATP and RecA (New England Biolabs, USA) as well as SSB gradients were added to the respective rows (Tab ...
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bioRxiv - Microbiology 2023Quote: ... 250 μL of sample with 16 μg/mL TPCK-treated trypsin (New England BioLabs), 2X penicillin and streptomycin and 2X antibiotic-antimycotic (Wisent ...
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bioRxiv - Biochemistry 2020Quote: ... 1.5 mg of pNL003 plasmid (38) was linearized by incubation at 37 °C for 2-4 h in the presence of EcoRV (NEB R0195S). Transcription reactions (10 mL ...
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bioRxiv - Molecular Biology 2020Quote: ... then incubated for 2 h at 37°C in 100 μl 1x TdT buffer containing 4 μl 10 mM ATP and 2 μl Terminal Transferase (NEB M0315L). Plugs were rinsed with 1 ml tris buffer (10 mM Tris HCl pH 8.0) ...
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bioRxiv - Neuroscience 2023Quote: ... the coverslips were washed in 2×SSC for 30 minutes for a total of four washes and then stored at 4°C in 2×SSC supplemented with 1:100 Murine RNase inhibitor (New England Biolabs, M0314S) for no longer than 2 weeks prior to imaging.
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bioRxiv - Genomics 2023Quote: ... cells were then pelleted at 500xg for 2 min at 4°C and then resuspended in 200 μl of 1 × T4 DNA ligase buffer (NEB, B0202S) containing 0.2% SDS ...
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bioRxiv - Synthetic Biology 2019Quote: ... An in vitro RED20 prototype was prepared by supplementing PURExpress in vitro expression system lacking tRNAs (PURE ΔtRNA, NEB# E6840S). PURE ΔtRNA supplemented with supplied control tRNAs was used as a Standard Code ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 nM Ca2+ and 10 μM purified Calmodulin (CaM) in the coupled transcription/translation PURE system (New England Biolabs, USA), which contains a defined set of purified translation factors and E ...
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bioRxiv - Cell Biology 2021Quote: ... 20µg of protein lysates and media samples were incubated with glycoprotein denaturation buffer at 95°C for 10 min and treated with 0.3µl Endo H (NEB; Catalog no #P0702) enzyme in glyco-buffer 3 for one hour at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from the heads of 20 male adults or 17 female adults using a Monarch Total RNA Miniprep Kit (NEB). 3 replicates for male and 3 replicates for female flies were done for each genotype ...
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bioRxiv - Microbiology 2020Quote: ... Circularised product is subjected to two rounds of rRNA depletion using biotinylated oligos [17] and streptavidin-coated magnetic beads (NEB) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The rRNA-subtracted circular product is subjected to a final round of PCR amplification with Illumina adaptor primers [17] using Phusion polymerase (NEB). All the libraries were then quantified ...
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bioRxiv - Cell Biology 2023Quote: ... sgRNA cassettes were isolated by two rounds of PCR using a previously published strategy (17) with NEBNext Ultra II Q5 Master Mix (New England Biolabs) and primer pairs listed in Extended Data Table 2.
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bioRxiv - Biophysics 2020Quote: The cell-free transcription/translation mix used in this study is a customized version of the PURE system (40) without ribosomes (PUREexpress Δ ribosomes, New England Biolabs) and the amino acid histidine ...
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bioRxiv - Synthetic Biology 2022Quote: ... and eluted in 10 μL of Ultra Pure™ DNase/RNase free distilled water: the purified amplicon and the 1kb DNA ladder (NEB) were run on 1% agarose gel in 1x TBE for 1 h 30 min at 110 V ...
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bioRxiv - Synthetic Biology 2022Quote: ... and eluted in 10 μL of Ultra Pure™ DNase/RNase free distilled water: the purified amplicon and the low molecular weight DNA ladder (NEB) were run on 2% agarose gel in 1x TBE for 1 h 40 min at 100 V ...
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bioRxiv - Microbiology 2023Quote: ... 4% DMSO (Biolabs), 200 nM of each primer kdpAB(37) ...
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bioRxiv - Biochemistry 2023Quote: ... buffer 4 (NEB, identical composition to rCutsmart buffer ...
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bioRxiv - Biophysics 2022Quote: ... the sample was ligated overnight at 16°C using T4 DNA ligase (New England BioLabs).
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bioRxiv - Biochemistry 2019Quote: ... The cDNA was amplified for 6-16 cycles (primers below) with Phusion Polymerase (NEB, #E0553L) and gel-purified with ZymoClean Gel DNA Recovery Kit (Genesee Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... for 16 cycles annealing at 67 °C was mixed with 1 μL of ExoI (NEB) digested mixture as the template ...
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bioRxiv - Neuroscience 2019Quote: ... exon 16 of APP was amplified and initially screened by restriction digest with Xba1 (NEB). Sanger sequencing was used to confirm the mutation ...
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bioRxiv - Molecular Biology 2021Quote: ... digested genomic DNA by O/N incubation at 16°C with T4 DNA ligase (NEB). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... and 16s rRNA transcripts by the Luna Universal One-Step RT-qPCR kit (NEB, E3005), according to the manufacturer’s instructions on an Applied Biosystems 7500 Fast Real-Time PCR System ...
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bioRxiv - Molecular Biology 2022Quote: ... and ligated over night at 16°C using T4 DNA ligase (New England Biolabs, UK). The ligation mix was transformed into E ...
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bioRxiv - Bioengineering 2021Quote: ... dsDNA fragments were inserted into PCR-amplified (primers 17-18 in Table S3) and purified pRSET vector fragment by Gibson Assembly (NEB, USA) to add sequence parts necessary for cDNA display generation to the DNA library ...
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bioRxiv - Microbiology 2021Quote: ... the sgRNA-encoding region was amplified from 500 ng genomic DNA with 17 cycles of PCR using NEBNext Ultra II Q5 master Mix (NEB #M0544L). Each PCR reaction contained a pool of forward primers (0.5 μM final concentration ...
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bioRxiv - Microbiology 2022Quote: ... The sgRNA-encoding region was amplified from 500ng genomic DNA with 17 cycles of PCR using NEBNext Ultra II Q5 master mix (NEB M0544L) as described in ...
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bioRxiv - Genetics 2019Quote: ... DBT1 loci were first amplified with 17 cycles of PCR using a touchdown protocol and the NEBnext 2x master mix (New England Biolabs M0541). The resulting product served as input to a second PCR ...
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bioRxiv - Physiology 2020Quote: ... Mutagenesis of Ser125 to Arg in GPT and Ser153 to Arg in GPT2 17 was performed with the Q5 site-directed mutagenesis kit from NEB (#E0554). Adeno-associated viruses (AAV ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μL GlycoBuffer 2 10X (NEB), 2 μL NP-40 ...
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bioRxiv - Neuroscience 2022Quote: ... 150 ng of RNA per sample was employed in the generation of libraries using the NEB Next Ultra Pure RNA library kit for Illumina (NEB, cat # E7770S) per manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... 500nM gene specific or 65nM 16S rRNA primer and 0.5 units Hot Start Taq Polymerase (NEB), and 10ng of template cDNA ...
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bioRxiv - Genomics 2019Quote: ... then incubated with 16 µg of TET2 enzyme (EM-seq component E7130A, NEB, Ipswich MA, USA) for 30 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... 500nM gene specific or 65nM 16S rRNA primer and 0.5 units Hot Start Taq Polymerase (NEB), and 10ng of template cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... and were ligated over night at 16 °C using T4 DNA ligase (New England Biolabs, UK). The ligation mix was transformed into E ...
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bioRxiv - Molecular Biology 2022Quote: ... and were ligated over night at 16 °C using T4 DNA ligase (New England Biolabs, UK). The ligation mix was transformed into E ...
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bioRxiv - Plant Biology 2020Quote: ... A total of 16 µg genomic DNA was digested with HindIII restriction endonuclease (NEB Biolabs, UAS). Digested DNA was separated by electrophoresis on a 0.8 % agarose gel and then transferred onto Hybond N+ membrane (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... A total of 16 µg genomic DNA was digested with HindIII restriction endonuclease (NEB Biolabs, UAS). Digested DNA was separated by electrophoresis on a 0.8 % agarose gel and then transferred onto Hybond N+ membrane (GE Healthcare ...
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bioRxiv - Genomics 2021Quote: ... followed by an overnight incubation at 16°C with 3 µL T4 DNA ligase (NEB, M0202). Samples were purified with phenol-chloroform and used as 3C templates for Taqman-qPCR ...
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bioRxiv - Cell Biology 2021Quote: ... Lis1 was first covalently coupled to 16 μL of SNAP-Capture Magnetic Beads (New England Biolabs) in 2 mL Protein Lo Bind Tubes (Eppendorf ...
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bioRxiv - Microbiology 2020Quote: 16S ribosomal DNA templates (∼1,465 bp) were amplified using Q5 high fidelity PCR (New England Biolabs) with the universal primer set 27F (5’-AGAGTTTGATCCTGGCTCAG-3’ ...