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Citations for New England Biolabs :
151 - 200 of 2243 citations for Estriol 2 4 16 17 D4 98% 95% Pure since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Plasmid and insert were then ligated overnight at 16 °C (T4 DNA ligase, New England Biolabs). After transformation into chemically competent E.coli and subsequent plasmid purification ...
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bioRxiv - Microbiology 2023Quote: ... Vector and insert were ligated overnight at 16°C using T4 DNA ligase (New England Biolabs) and transformed into E ...
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bioRxiv - Cell Biology 2022Quote: ... End-repair/A-tailing was performed on 17 μL of ChIPed DNA using NEBNext® Ultra™ II End Repair/dA-Tailing Module (New England BioLabs), followed by ligation (MNase_F/MNase_R ...
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bioRxiv - Microbiology 2020Quote: ... pCrPV-1A-DcDV was linearized by inverse PCR with primers 17 and 18 and the linearized plasmid was circularized by blunt end ligation with T4 DNA ligase (New England Biolabs, Ipswich, Massachusetts) according to the manufacturer’s instructions to create pCrPV-1A-DcDV-1A ...
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bioRxiv - Bioengineering 2022Quote: ... End-repair/A-tailing was performed on 17 μL of ChIPed DNA using NEBNext® Ultra™ II End Repair/dA-Tailing Module (New England BioLabs), followed by ligation (MNase_F/MNase_R ...
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bioRxiv - Molecular Biology 2022Quote: ... end-repair/A-tailing was performed on 17 µL of ChIPed DNA using NEBNext® Ultra™ II End Repair/dA-Tailing Module (New England BioLabs), followed by adapter ligation using T4 DNA Ligase (New England BioLabs) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 4: 4°C hold) using NEB Next High-Fidelity master mix (NEB) in 25 µl reaction volume using RAD-Marker for/ RAD-Marker rev primers (25 nM each ...
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bioRxiv - Cell Biology 2020Quote: ... Oct-4 (NEB, D7O5Z), Sox2 (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 ml ScaI (BioLabs), followed by 3 h of incubation with a fresh portion ...
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bioRxiv - Biochemistry 2021Quote: ... and one plasmid was randomly selected from those plasmids and used for expression with a PURE system (PURExpress In Vitro Protein Synthesis Kit, New England BioLabs, Ipswich, MA, USA).
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bioRxiv - Microbiology 2020Quote: ... PCR protocols entailed an initial phase of 95° C for 3 min using Hot Start Taq polymerase (New England BioLabs Inc., Ipswich, MA, USA), followed by 35 cycles at 50°C and 52°C annealing temperatures for 16S and ITS amplicons respectively ...
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bioRxiv - Microbiology 2021Quote: ... Ligation to each splint was performed overnight at 16°C using T4 DNA ligase (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... Digested chromatin was then ligated at 16 °C with 10000 U of T4 DNA ligase (NEB, M0202) in ligase buffer supplemented with 10% Triton-100 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... 16 μg of sonicated DNA was subjected to RNase H (NEB, 1.5 U per μg of DNA) treatment or RNase H buffer only for 4 hr at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Digested chromatin was then diluted and ligated overnight at 16°C with T4 DNA ligase (NEB, M0202M). Reversal of crosslinks ...
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bioRxiv - Synthetic Biology 2022Quote: ... Restriction digested chunks were ligated over night at 16°C using T4 DNA ligase (New England Biolabs).
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bioRxiv - Genomics 2023Quote: ... This 17 µl of DNA solution was incubated with 3 µl digestion mixture containing 1 µl BciVI (New England Biolabs, cat. no. R0596S) and 2 µl CutSmart buffer (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μL of 2% SDS and 2 μL of proteinase K (New England Biolabs) were added and the solution was incubated at 37°C for 1 hour before purification with the Qiagen DNA clean up kit ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 μl 10X NEBuffer 2 (New England Biolabs) and nuclease-free water (total of 20 μl ...
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bioRxiv - Genomics 2023Quote: ... nuclei from 2 million MCF-7/NA12878 cells at a viability of ∼95% were resuspended in 200 μl of reaction buffer (1× NEB CutSmart buffer, 0.3 M sucrose). Nuclei were then treated with EcoGII by adding 200 U of EcoGII (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... SSS reaction products (16 μL) were combined with 24 μL HiScribe T7 High Yield RNA Synthesis Kit (NEB) reagent mix containing 4 μL T7 Buffer ...
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bioRxiv - Microbiology 2019Quote: ... For the 16S enrichment we performed a PCR with Q5® High-Fidelity DNA Polymerase (New England Biolabs), the forward primer 341F and the reverse primer 806R to cover the V3/V4 region ...
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bioRxiv - Molecular Biology 2020Quote: ... Around 500ng of RNA were ligated overnight at 16°C using 20units of T4 RNA ligase 1 (NEB) in the presence of 10% PEG 8000 to favour intramolecular ligations ...
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bioRxiv - Molecular Biology 2020Quote: ... Around 500ng of RNA were ligated overnight at 16°C using 20units of T4 RNA ligase 1 (NEB) in the presence of 10% PEG 8000 to favour intramolecular ligations ...
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bioRxiv - Genetics 2020Quote: ... Pairs of annealed oligos and the digested vector were ligated overnight at 16°C using T4 ligase (NEB). Following transformation ...
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bioRxiv - Developmental Biology 2020Quote: ... 16 ng/uL single-stranded pooled oligos were amplified with NEBNext High-Fidelity 2X PCR Master Mix (NEB) with the following PCR protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... We performed the isolation of 16 random clones and triple restriction enzyme digestion using BsrGI-HF (NEB #R3575S), ClaI (NEB #R0197S) ...
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bioRxiv - Microbiology 2022Quote: ... Inserts were ligated into the linearized plasmid overnight at 16°C using T4 DNA ligase (New England Biolabs) following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: ... The linearized vector and the sgRNAs heteroduplexes were ligated overnight at 16°C using T4 DNA ligase (NEB), and the ligation product was used to transform One Shot™ Stbl3™ cells (Thermo Fisher) ...
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bioRxiv - Genomics 2023Quote: ... The vectors and insert guides were annealed for 16 hrs overnight using T4 ligase (New England Biolabs, M0202L) at a 1:2 molar ratio of vector to insert ...
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bioRxiv - Systems Biology 2024Quote: ... at 16 °C overnight, followed by heat-inactivation (65°C, 15 min) and linearization using SbfI-HF (NEB) at 37°C for 2 h ...
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bioRxiv - Microbiology 2023Quote: All the 16S rRNA used for nanopore sequencing were treated with polyA polymerase (New England Biolabs, Beverly, MA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Ligations into the respective backbone were performed overnight at 16°C using T4 DNA Ligase Kit (NEB, #M0202L). Ligations were transformed into NEB Stable E ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μL of 2 U L-1 ExoI (NEB) was added and incubated at 37 °C for 30 minutes then 80 °C for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μL of GlycoBuffer 2 (NEB Cat # B0701S, 10X), 2 μL of 10% NP-40 (NEB Cat # B0701S ...
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bioRxiv - Systems Biology 2024Quote: ... 2 µl of NEBuffer 2 (New England Biolabs B7002) and 1 µl of Klenow large fragment DNA polymerase (New England Biolabs M0210 ...
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bioRxiv - Genomics 2019Quote: ... and 4 U MmeI (NEB) for 2 h at 37 °C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 1×Buffer 4 (NEB). The reaction was stopped (6 mM EGTA ...
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bioRxiv - Genomics 2022Quote: ... 6.25x NEBuffer 4 (NEB, B7004S)) was added to each well ...
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bioRxiv - Molecular Biology 2023Quote: ... and 4 mM SAM (NEB). RNAs were then purified with the RNA Clean and Concentrator-5 Kit (Zymo) ...
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bioRxiv - Genomics 2023Quote: ... 1.5 μL NEBuffer 4 (NEB), 0.75 μL T4 Phage β-glucosyltransferase (NEB M0357S) ...
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bioRxiv - Genomics 2020Quote: ... nuclei were centrifuged 5min at 3,000 rpm and ligation was performed 4h at 16°C using 10,000 cohesive end units of T4 DNA ligase (NEB) in 1.2ml of ligation buffer (120μl of 10X T4 DNA Ligase Buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... SSS reaction products (16 μl) were combined with 24 μl T7 High Yield RNA Synthesis Kit (NEB, Cat #E2040) reagent mix containing 4 μl T7 Buffer ...
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bioRxiv - Genomics 2019Quote: ... 16 libraries for each histone mark) using NEBNext Ultra II DNA Library Prep kit for Illumina (NEB, cat. # E7645). NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Microbiology 2020Quote: ... 16S amplicon from all samples was prepared for sequencing using the NEBNext Illumina library preparation kit (New England Biolabs), and sequenced with 400 forward cycles of v2 Miseq chemistry.
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bioRxiv - Plant Biology 2021Quote: ... Digested DNA was ligated during 5 h incubation at 16 °C with 100 U of T4 DNA ligase (NEB). DNA was recovered after reverse crosslinking and Proteinase K treatment (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial 16S rRNA V4 fragments were PCR amplified using NEBNext Q5 Hot Start HiFi PCR master mix (NEB, U.K.) with universal 16S rRNA V4 primers [564F (TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-AYTGGGYDTAAAGNG ...
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bioRxiv - Systems Biology 2019Quote: ... the beads were incubated 16h at 16°C in 1xPNK buffer with 40U T4 RNA ligase I (NEB, M0204L), and 1 μl 100 μM L5 adapter (Supplementary Table 5) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 8 μL of solution A and 6 μL of solution B were mixed with 16 U RNase Inhibitor (NEB), gBlock templates (2 nM each ...
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bioRxiv - Developmental Biology 2023Quote: ... 3 μL of Round1 barcode mix (1x T4 DNA ligase buffer, 16 M/μL T4 DNA ligase (M0202L, NEB), 0.25 M/μL RNase Inhibitor ...