Labshake search
Citations for New England Biolabs :
2151 - 2200 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... pUltra-Hot-ZFAND6 was generated by subcloning a ZFAND6 PCR product digested with Xba1 (New England Biolabs; R0145S) and BamH1-HF (New England Biolabs ...
-
bioRxiv - Microbiology 2024Quote: ... All PCR products were amplified using Q5-High Fidelity DNA polymerase according to the manufacturer (New England Biolabs).
-
bioRxiv - Cancer Biology 2024Quote: ... The resulting PCR products then underwent end-repair and A-tail addition followed by New England Biolabs (NEB) adapter ligation ...
-
bioRxiv - Bioengineering 2023Quote: ... The upstream and downstream PCR products were digested by BssSI restriction enzyme (New England Biolabs, Ipswich, MA, USA). After another round of agarose electrophoresis and gel purification ...
-
bioRxiv - Neuroscience 2022Quote: ... PCR product was then restriction enzyme digested at 37° C for 1 hour with MseI (New England Biolabs) and then run on a 1.5% Agarose gel ...
-
bioRxiv - Genetics 2023Quote: ... PCR products were purified after agarose gel electrophoresis using the Monarch DNA Gel Extraction Kit (New England Biolabs) and submitted for Sanger sequencing to Eurofins Genomics ...
-
bioRxiv - Biochemistry 2023Quote: ... Purified PCR products were ligated using the HiFi DNA Assembly master mix (New England Biolabs, catalog no. E2621S) into a Blasticidin-resistance encoding lentiviral vector (Addgene ...
-
bioRxiv - Genomics 2023Quote: ... The recombination products were then electroporated into electrocompetent cells (NEB 10-beta, New England BioLabs, cat. No. C3020K) and plated onto Carbenicillin plates ...
-
bioRxiv - Genomics 2023Quote: ... the products of PCR1 were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, no. T1030L) and deep sequenced at the MGH DNA Core ...
-
bioRxiv - Cell Biology 2023Quote: ... ERK7-BioID2-3xHA was created by transfecting the RHΔku80Δhxgprt strain (66) with 50 μL of a PCR product using Q5 polymerase (NEB) with 500 bp homology arms flanking a BioID2-3xHA tag together with 5 μg of a Cas9 plasmid that had been modified to express HXGPRT and also a gRNA targeting the C-terminus of ERK7 (37) ...
-
bioRxiv - Microbiology 2023Quote: ... Purified PCR products were incubated together in a thermocycler with 1X Hi-Fi DNA Assembly master mix (NEB) at 50°C for one hour ...
-
bioRxiv - Cancer Biology 2023Quote: ... and the cleavage products were evaluated using a Reverse Mass DNA Ladder in Gel Loading Dye Orange (NEB).
-
bioRxiv - Plant Biology 2023Quote: ... Amplified PCR products with appropriate expected sizes were purified with the Monarch® DNA Gel Extraction Kit (NEB). Purified PCR products were cloned into the pMiniT 2.0 vector (NEB ...
-
bioRxiv - Bioengineering 2023Quote: ... The PCR products were digested using ClaI and BamHI in 1X Cutsmart buffer (New England Biolabs, Ipswich, MA) and ligated into the pCW57 backbone using T4 DNA ligase ...
-
bioRxiv - Cell Biology 2023Quote: ... The resulting product was transformed into then transformed into competent cells (C2987, New England BioLabs, Ipswich, MA, USA). Individual colonies were grown overnight under selection and DNA was extracted by miniprep according to the manufacturer’s instructions (FERK0503 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... the RCA product was resolved to linear monomers by restriction using the endonuclease Fsp1 (New England Biolabs, R0135L) under the similar conditions.
-
bioRxiv - Systems Biology 2023Quote: ... The concentrated ligated products were then transformed into NEB 10-beta Electrocompetent E.coli (New England Biolabs, Ipswitch, MA). Three transformations were performed to increase the yield ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5 μL of the polyC-tailed products were used with 1X Taq Mg-free Buffer (New England Biolabs), 500 nM of each primer (Table 2) ...
-
bioRxiv - Genomics 2023Quote: ... The product was converted to double stranded DNA using the NEBNext second strand synthesis kit (New England Biolabs). Library preparation was carried out using a KAPA LTP kit (KAPA Biosystems) ...
-
bioRxiv - Cell Biology 2023Quote: ... The resulting product was transformed into then transformed into competent cells (C2987, New England BioLabs, Ipswich, MA, USA). Individual colonies were grown overnight under selection and DNA was extracted by miniprep according to the manufacturer’s instructions (FERK0503 ...
-
bioRxiv - Genomics 2023Quote: ... The purified products were subjected to dA tailing with 2 μL of 10X NEBuffer 2 (New England BioLabs), 0.1 μL of 100 mM dATP ...
-
bioRxiv - Biochemistry 2024Quote: ... PCR products were analyzed by agarose gel electrophoresis and purified with Monarch PCR and DNA Cleanup Kit (NEB). On a passivated glass coverslip ...
-
bioRxiv - Synthetic Biology 2024Quote: ... extracted PCR product with 2 μl of NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2024Quote: ... the reaction products were nicked to remove supercoiling by incubation at 37°C with Nt.BsmAI or Nb.BssSI nicking endonucleases (NEB) within the existing reaction mixture ...
-
bioRxiv - Neuroscience 2024Quote: ... One PCR product from a successful KO was gel extracted using Monarch DNA Extraction Kit (NEB Cat # T1020) and set for sequencing ...
-
bioRxiv - Microbiology 2024Quote: Enriched RNA underwent library preparation using the NEBNext multiplex small RNA library prep kit (NEB product number E7300S) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... We purified the PCR-product with 1X AMPure XP beads and used Gibson Master mix (New England Biolabs) assemble the previously prepared vector backbone and purified Blasticidin PCR-amplified insert following Gibson assembly recommendations ...
-
bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted from each samples using the Monarch total RNA miniprep kit (NEB product number T2010S) TRIzol extraction protocol with on-column DNase treatment ...
-
bioRxiv - Molecular Biology 2024Quote: ... The product was purified again with Zymo columns and digested ON at 37°C with BcoDI (NEB, R0542). The digested product was resolved on a 1.8% agarose gel and the seed was gel purified (∼ 130bp product) ...
-
bioRxiv - Biophysics 2024Quote: ... Template digestion and PCR product ligation were completed using the KLD Enzyme Mix standard protocol (New England Biolabs). The PCR products were transformed into DH5α competent cells and incubated overnight on LB-agar plates containing 100μM ampicillin ...
-
bioRxiv - Bioengineering 2024Quote: ... The pNL4.3 and partial envelope PCR products were then fused using NEBuilder HiFi DNA Assembly (NEB, Ipswich MA) per the manufacturer’s recommendations to produce an NL4.3-env.ecto chimeric HIV infectious molecular clone (Env-IMC ...
-
bioRxiv - Cell Biology 2024Quote: ... PCR products harbouring PP1α87B coding sequence were cloned into StuI/XbaI sites of pMal-c2 (New England Biolabs) vector ...
-
bioRxiv - Cell Biology 2024Quote: ... The PCR products were cloned into each lentiviral vector overnight by classical ligation with T4 ligase (NEB, M0202S). The resulting ligation product was transformed in 25 µl of DH5α (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2024Quote: ... The 235 bp PCR product was digested with restriction enzyme Hpy188III (New England Biolabs, Ipswich, Massachusetts, cat# R0622S), The WT sequence remains uncut ...
-
bioRxiv - Cancer Biology 2024Quote: ... 20 µl of each PCR product were digested with 0.5 µl MslI (for Tp53R270H/+; New England BioLabs, R0571L) in a final reaction volume of 30 µl ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The PCR products were incubated at 37°C with 1ml of DpnI (R0176S, New England BioLabs, Ipswich, MA) and then purified with NucleoFast 96 PCR Plates (743100.10 ...
-
bioRxiv - Biophysics 2021Quote: ... The PCR1 reactions were run using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2021Quote: ... The GRB2 mutagenesis plasmid (pGJJ057) was assembled by a ligation reaction (T4 Ligase, New England Biolabs) following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... by individual mutagenic PCR reactions combined with NEBuilder® HiFi Assembly (New England Biolabs, Ipswich, MA). Plasmid pSM3393 was the template for all reactions ...
-
bioRxiv - Cell Biology 2020Quote: ... and circularized by an intramolecular ligation reaction using the Quick Ligation™ Kit (New England Biolabs)
-
bioRxiv - Microbiology 2021Quote: ... Each sample was amplified separately by two PCR reactions using Q5 high-fidelity polymerase (NEB, M0149S) and zone-specific PCR primers ...
-
bioRxiv - Developmental Biology 2021Quote: ... The 20 μl of the A-tailing reaction were mixed with Quick Ligase buffer 2X (NEB), 3000 U of Quick Ligase (NEB ...
-
bioRxiv - Genomics 2020Quote: The MDA reaction mixture was prepared with phi29 DNA Polymerase (New England BioLabs catalog no. M0269L) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1µg per sample of Trizol extracted RNA were treated with 0.15µl/reaction of DNase I (NEB) and 1µl 10x DNase buffer in a 10µl reaction for 30mins at 37 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... mutation of SSL2 (ssl2*) was accomplished by standard PCR reactions using Taq polymerase (New England Biolabs). ssl2* PCR products were then transformed into yeast along with a linearized pRS315 SSL2 LEU2 plasmid with most of the wild-type SSL2 sequence removed by restriction digest ...
-
bioRxiv - Cell Biology 2020Quote: ... 4µl of synthesized cDNA mixtures were used in 25µl reactions with Taq polymerase (New England Biolabs). For normalization ...
-
bioRxiv - Cancer Biology 2020Quote: ... 20 μl reaction mixture composed by 10 μl of Phusion High-Fidelity Master Mix 2X (NEB), 2 μl of Betaine 5M (Sigma-Aldrich) ...
-
bioRxiv - Physiology 2021Quote: ... RNA 3’ end dephosphorylation reaction consisted of T4 PNK (20 U/10 μL sample, NEB M0201S), SuperaseIn in 1X T4 PNK buffer without ATP for 60 min at 37 °C ...
-
bioRxiv - Genomics 2020Quote: ... This involved digesting 250ng of DNA per sample in a 50ul reaction with PstI-HF (NEB) for 1 hour at 37C ...
-
bioRxiv - Systems Biology 2020Quote: ... was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen ...