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Citations for New England Biolabs :
1651 - 1700 of 4746 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Standard Q5 Hot Start High-Fidelity Master Mix (New England Biolabs M0494S) was used to amplify the pBBR1 backbone from the pBHV-KanR plasmid (P1 ...
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bioRxiv - Cell Biology 2021Quote: ... using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Cat# E2621L) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... and Phusion HF Master Mix (New England BioLabs, Inc., Ipswich, MA, USA) with the following protocol ...
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bioRxiv - Microbiology 2020Quote: ... PCR (NEBNext Ultra II Q5 ® Master Mix, NEB, Ipswich, MA, USA) was performed with a gene-specific forward primer designed 700-nt upstream of the apparent boundary between the SARS-CoV-2 genome body and the putative poly-A tail ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were indexed using NEBNext High-Fidelity PCR Master Mix (NEB M0541S) for 11 cycles (size-matched input ...
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bioRxiv - Microbiology 2021Quote: ... All cloning was completed using Gibson Assembly Master Mix (New England Biolabs) for isothermal DNA assembly (57) ...
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bioRxiv - Genetics 2021Quote: ... This fused cassette was obtained using Gibson Assembly® Master Mix (NEB) following the manufacturer’s instructions and the primers used to generate the assembly fragments were designed using NEBuilder Assembly Tool (https://nebuilder.neb.com) ...
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bioRxiv - Genetics 2021Quote: ... pGAD-rad55-S2,8,14A was generated using Gibson Assembly® Master Mix (NEB) following the manufacturer’s instructions and the primers used to generate the assembly fragments were designed using NEBuilder Assembly Tool (https://nebuilder.neb.com) ...
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bioRxiv - Genetics 2020Quote: ... and mixed with 25 μl blunt/TA ligase master mix (NEB M0367). The mixture was incubated at room temperature for 15 min ...
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bioRxiv - Systems Biology 2021Quote: ... the commercial OneTaq™ master mix (New England BioLabs; Ipswich, MA, USA) was used according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... PCR amplifications were carried out using 2 x Q5 Master Mix (NEB), with cycling times and temperatures according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Amplicons were generated using Q5 PCR Master Mix (NEB, Ipswich, MA, USA), and the resulting PCR products were cleaned using a NucleoSpin Gel and PCR Clean-Up kit (Macherey-Nagel ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... PCR amplification was performed using Q5 hotstart high fidelity master mix (NEB) with respective primers and 2 ng of Plasmid pKR72 as template DNA ...
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bioRxiv - Immunology 2022Quote: ... Gibson assembly was performed using a Gibson master mix (New England Biolabs), 50ng of vector ...
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bioRxiv - Genomics 2022Quote: ... 25 μl NEBNext® Ultra™ II Q5® Master Mix (NEB) and the whole purified DNA sample (5 min 72°C ...
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bioRxiv - Genetics 2022Quote: ... Eluted cDNA was amplified 5-cycles (NEBNextµltra II Q5 master mix (NEB) with Illumina TruSeq PCR primers RP-1 and RPI-X ...
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bioRxiv - Cell Biology 2021Quote: ... and NEBNext High-Fidelity 2xPCR Master Mix (New England Biolabs, Ipswich, Massachusetts) for 5 cycles ...
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bioRxiv - Microbiology 2020Quote: ... were assembled together using Gibson Assembly® Master Mix (New England Biolabs) at 50 °C for two hours ...
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bioRxiv - Genetics 2022Quote: ... 25 µL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 20 µL nuclease-free water ...
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bioRxiv - Genomics 2022Quote: ... Eluted DNA was amplified using NEBNext Ultra II PCR Master Mix (NEB) and purified using AMPure XP beads ...
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bioRxiv - Immunology 2022Quote: ... a quantitative PCR reaction (10 µl 2 x PCR master mix (NEB), 1 x SYBR green (Thermo Fisher) ...
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bioRxiv - Cell Biology 2022Quote: Cloning was performed with the Gibson Assembly Master Mix (New England Biolabs). PCR products were produced with the Q5 High-Fidelity 2x Master Mix (New England Biolabs) ...
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bioRxiv - Genomics 2022Quote: ... we used NEB HiFi DNA Assembly Master Mix (New England Biolabs E2621) to insert the U6 promoter between the two copies of the cBCs where it drives expression of the downstream copy of the cBC ...
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bioRxiv - Immunology 2022Quote: ... and cloning was performed using the Gibson Assembly 2× Master Mix (NEB) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: FastPfu High-Fidelity Master Mix was purchased from New England BioLabs (NEB). TIAN Prep Rapid Mini Plasmid Kit was purchased from TIANGEN ...
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bioRxiv - Developmental Biology 2020Quote: ... and amplification was performed in 1 × next PCR master mix (NEB, M0541S) and 1.25 μM of custom Nextera PCR primers 1 and 2 with the following PCR conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... The reactions were performed using NEBuilder HiFi DNA Assembly Master Mix (NEB) by incubation at 50 °C for 15 minutes ...
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bioRxiv - Synthetic Biology 2020Quote: ... Each reporter was assembled with Gibson Assembly® Master Mix (NEB #E2611S) using 30 bp overlaps between the plasmid pLibAcceptorV2 ...
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bioRxiv - Molecular Biology 2020Quote: ... T4 DNA ligase and Gibson Assembly Master Mix were purchased from NEB, and digests and ligations were performed according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, MA), followed by allelic exchange ...
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bioRxiv - Genomics 2021Quote: ... 12.5 µL of NEBNext Ultra II Q5 master mix (NEB, Cat #M0544L), and H2O to a total 25 µL reaction volume ...
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bioRxiv - Cell Biology 2021Quote: ... by Gibson assembly using the Gibson Assembly Master Mix (New England Biolabs).The linker between Tau and the fluorescent protein was composed of 21 amino acids in all cases (YPYGILQSTVPRARDPPVATA/M for YFP and mTurquoise2 plasmids ...
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bioRxiv - Cell Biology 2021Quote: ... Ligation master mix (398 μL of water, 50 μL of 10 × NEB T4 DNA ligase buffer ...
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bioRxiv - Cell Biology 2020Quote: All plasmids were constructed using NEBuilder HiFi DNA assembly master mix (NEB) with PCR products ...
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bioRxiv - Molecular Biology 2021Quote: ... A PCR cocktail consisting of NEBNext Ultra II Q5 master mix (NEB) and Illumina TruSeq PCR primers (RP-1 ...
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bioRxiv - Genomics 2021Quote: ... we used NEBNext® Ultra™ II Q5® Master Mix (NEB). This master mix contains Q5® High Fidelity DNA Polymerase ...
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bioRxiv - Genomics 2021Quote: ... For Smartseq3xpress with NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB), PCR mastermix consisted of (1x NEBNext Q5 Hot Start HiFi PCR Master Mix ...
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bioRxiv - Molecular Biology 2020Quote: ... vector by Gibson assembly (NEBuilder® HiFi DNA Assembly Master Mix; NEB). Gli protein constructs were tagged with the N-terminal 3xHA tag in tandem with the TEV protease target sequence ...
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bioRxiv - Synthetic Biology 2022Quote: ... The Golden Gate Assembly Master Mix BsaI-HF v1 and v2 (NEB) was used to construct Level 1 plasmids.
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bioRxiv - Genomics 2022Quote: NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (E6240L, NEB)
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bioRxiv - Genomics 2022Quote: ... using NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (NEB) and NEBNext Ultra DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Genomics 2022Quote: ... Eluted DNA was amplified using NEBNext Ultra II PCR Master Mix (NEB) and purified using AMPure XP beads ...
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bioRxiv - Synthetic Biology 2022Quote: ... Gibson assembly was performed using NEBuilder HiFi DNA Assembly Master Mix (NEB) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were subsequently PCR amplified using Phusion HF PCR Master Mix (NEB) with the following PCR program ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmid assembly was performed using NEBuilder HiFi DNA Assembly Master Mix (NEB), followed by transformation into NEB 5-alpha competent E ...
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bioRxiv - Molecular Biology 2022Quote: ... by adding 25 μl of NEBNext Ultra II Q5 Master Mix (NEB), 11 μl Nuclease free water and 2 μl of RP1 and RPIx primers (10 μM) ...
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bioRxiv - Microbiology 2023Quote: ... and assembly was performed using the HiFi DNA assembly master mix (NEB). The digested assembly reaction was amplified by rolling circle amplification using the Repli-g mini kit (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... A polymerase chain reaction (PCR) amplification using OneTaq Master Mix (NEB, M0482) was performed on C ...
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bioRxiv - Developmental Biology 2023Quote: ... 2uL 10uM uniquely barcoded i7 primer and 25uL PCR master mix (NEB Phusion® High-Fidelity PCR Master Mix with HF Buffer ...
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bioRxiv - Biochemistry 2022Quote: ... prepared in Phusion high-fidelity master mix with high-fidelity buffer (NEB), were run and then phosphorylated and re-ligated using T4 polynucleotide kinase (NEB ...