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Citations for New England Biolabs :
1801 - 1850 of 6017 citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... RNA-seq libraries were prepared from the mRNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Cluster generation was performed with the resulting libraries using the Illumina TruSeq PE Cluster Kit v4 reagents and sequenced on the Illumina HiSeq 2500 using TruSeq SBS Kit v4 reagents (Illumina) ...
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bioRxiv - Molecular Biology 2019Quote: Libraries were prepared with NEBNEXT rRNA Depletion kit (human/mouse/rat) and NEBNEXT Ultra II Directional RNA Library Prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... ChIP enrichment was determined by RT-qPCR (Supplementary Table 5) prior to addition of sequencing adaptors using NEBNext Ultra II DNA Library Prep kit for Illumina (New England Biolabs). ATAC-seq was performed as previously described76 ...
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bioRxiv - Genetics 2020Quote: ... all wells from a single plate were pooled and prepared for sequencing with the NEBNext Ultra II DNA Library Prep kit for Illumina (New England Biolabs). Plates were multiplexed and sequenced on the MiSeq platform (Illumina ...
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bioRxiv - Genomics 2019Quote: ... or 100 ng of gDNA was assembled into paired-end libraries using the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs). Approximately 60 libraries were pooled per lane prior to sequencing on a HiSeq 4000 machine to a target depth of 0.5×.
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bioRxiv - Microbiology 2019Quote: ... Insert and dual indexed libraries were prepared using a modified NEBNext Ultra DNA II dual indexing kit (New England Biolabs) without size selection ...
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bioRxiv - Genomics 2019Quote: ... Immunoprecipitated and input material was phenol-chloroform purified and ChIP-seq libraries were prepared using the NEBNext Ultra II DNA library kit (NEB) according to the manufacturer’s instructions with 35ng of DNA of each sample ...
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bioRxiv - Genomics 2019Quote: ... RNA-seq libraries were prepared using NEB’s NEBNext Ultra II Directional RNA Library Prep Kit for Illumina Sequencing (NEB EE7760S) and following the instructions for the poly(A ...
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bioRxiv - Genomics 2020Quote: ... NEBNext Ultra II End-prep reaction buffer (7 μl) and NEBNext Ultra II End-prep enzyme mix (3 μl, both from New England Biolabs) were added to each sample ...
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bioRxiv - Genetics 2020Quote: ... CUT&RUN dsDNA samples were quantified with a Qubit III and 5-10ng used as input for library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (#E7645S, NEB). Libraries were prepared using the following PCR program ...
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bioRxiv - Genomics 2021Quote: Wild-type HAP1 samples were used for standard ChIP-seq library preparation with NEBNext Ultra II DNA Library Prep kit (New England Biolabs), followed by sequencing on NovaSeq 6000 ...
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bioRxiv - Genomics 2021Quote: ... Shotgun sequencing was performed using a PCR-free DNA library preparation (NEBNext Ultra II DNA Library Prep Kit, New England Biolabs). Libraries were paired end 150 bp sequenced with Illumina HiSeqX ...
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bioRxiv - Genetics 2019Quote: ... and used to construct DNA sequencing libraries using NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs E7760). DNA libraries were processed on a Illumina NextSeq machine for paired-end 42-nt sequencing ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and prepared DNA-Seq libraries from 100 ng DNA using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs; 12 minutes enzymatic shearing ...
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bioRxiv - Genomics 2021Quote: ... or the NEBNext Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (both New England Biolabs).
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bioRxiv - Cancer Biology 2021Quote: ... Qualifying samples (RNA Integrity Number ≥ 9) were then prepped following the standard protocol for the NEB next Ultra ii Stranded mRNA (New England Biolabs). Sequencing was performed on the Illumina NextSeq 500 with Paired End 42bp × 42bp reads ...
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bioRxiv - Microbiology 2021Quote: ... and TruSeq-barcoded RNAseq libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Sequencing was performed at the Cornell University Transcriptional Regulation and Expression Facility on a NextSeq500 instrument (Illumina ...
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bioRxiv - Physiology 2021Quote: ... Three mRNA-seq libraries per condition were prepared using the NEBNext Ultra II directional RNA library Prep kit for Illumina (New England Biolabs), and read in paired-end 40-cycle sequencing runs on the NextSeq 500 system (Illumina) ...
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bioRxiv - Cell Biology 2020Quote: ... Second-strand cDNA synthesis was performed using NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs) and amplified by in vitro transcription (HiScribe ...
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bioRxiv - Microbiology 2020Quote: ... metagenomic libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit (New England Biolabs, Cat. No. E7645) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A library with 300-400 bp insert size for each pool was prepared using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs). Libraries were then sequenced with 150 bp paired-end on the HiSeq X Ten platform.
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bioRxiv - Biochemistry 2020Quote: DNA fragments containing sgRNA sequences were amplified from isolated genomic DNA by two rounds of PCR using NEBNext Ultra II Q5 master mix (NEB). For the first round of PCR ...
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bioRxiv - Biochemistry 2021Quote: ... End-repair and dA tailing were performed using NEBNext® Ultra− II End Repair/dA-Tailing Module (New England Biolabs) and the DNA ligated with a dT tailed chloramphenicol cassette ...
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bioRxiv - Biochemistry 2020Quote: ... Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA). Paired-end 100bp reads were performed on an Illumina HiSeq4000 ...
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bioRxiv - Microbiology 2021Quote: ... Fragmented poly-A RNA samples were converted to cDNA by random primed synthesis using ProtoScript II reverse transcriptase (New England Biolabs). After second strand synthesis ...
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bioRxiv - Genomics 2021Quote: ... The repaired DNA was end-prepped with the NEBNext Ultra II End Repair/dA-Tailling Module (NEB, Ipswich, Ma, USA), for 30 min at 20°C and 30 min at 65 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... ChIP and input DNA libraries were prepared for amplification by using the NEBNext Ultra II ChIP-seq Library Prep Master Mix Set for Illumina (NEB) following manufacturer’s protocols with no modifications ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples with RIN value higher than 8 were processed using the NEBNext poly(A) mRNA Magnetic Isolation kit and the NEBNext Ultra II RNA Library Prep kit for Illumina (New England Biolabs), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The procedure of the complementary DNA (cDNA) libraries was carried out with NEBNext Ultra II RNA library Prep kit (NEB) and NEBNextplex Oligos for Illumina following a previously described method (Kohno et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... WGS libraries were amplified using NEBNext® Ultra™ II Q5® Master Mix and index primers (New England Biolabs). Library purification was performed with TIANgel Midi Purification Kits (TIANGEN Biotech) ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were generated using a NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Cat No. E7645), including fragmentation with a Covaris S220 sonic disruptor ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were then prepared from 500 ng genomic DNA using the NEBNext Ultra II FS DNA library prep kit for Illumina (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... About 10ng of the purified CUT&RUN DNA was used for preparation of multiplexed libraries with the NEB Ultra II DNA Library Prep Kit per manufacturer’s instruction (NEB #E7103). Sequencing was conducted using an Illumina NextSeq 500 Sequencing System (available from the core facility of UNC Pharmacology Department).
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bioRxiv - Neuroscience 2021Quote: RT-PCR was conducted with 15ng cDNA input in a 100ul reaction using NEBNext Ultra II Q5 Master Mix (New England Biolabs), with resulting products visualized on a 1.5% TAE gel ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were constructed with the NEBNext® Ultra™ II DNA Library Prep kit (New England Biolabs, MA, USA), and sequenced by Illumina-MiSeq at the Pittsburgh Bacteriophage Institute to an approximate shotgun coverage of 3538x ...
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bioRxiv - Plant Biology 2020Quote: Sequencing libraries were generated from poly(A)-enriched RNA using the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Systems Biology 2020Quote: ... Library preparation was performed using the NEBNext®Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs), and library quantification was performed using the KAPA library quantification kit (Kapa Biosystems) ...
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bioRxiv - Plant Biology 2021Quote: ... The 35S:RAM1 cassette from pK7WG2R was amplified using the primer pair listed in Table S1 and cloned into pK7GWIWG2(II)-RedRoot at the Kpn1 and Xho1 sites using T4 DNA ligase (NEB). The vectors were transformed into M ...
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bioRxiv - Plant Biology 2021Quote: ... and the purified mRNA was used to build sequencing libraries using the Ultra II Directional RNA library Prep Kit for Illumina (NEB). Single end sequencing of 76 bp were performed on NextSeq instrument (Illumina) ...
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bioRxiv - Microbiology 2020Quote: ... by PCR amplification of cDNA generated using reverse transcribed from total cellular RNA isolated from C636 Aedes albopictus cells using Protoscript II RT (NEB) and oligo-dT primers (IDT) ...
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bioRxiv - Genomics 2020Quote: ... Samples were prepared for Illumina sequencing with NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (NEB #E7775) accompanied by NEBNext Poly(A ...
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Lessons from the meiotic recombination landscape of the ZMM deficient budding yeast Lachancea waltiibioRxiv - Genetics 2021Quote: ... DNA libraries were prepared from 5 ng of total genomic DNA using the NEBNext Ultra II FS DNA Library kit for Illumina (New England Biolabs). All volumes specified in the manufacturer’s protocol were divided by four ...
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bioRxiv - Neuroscience 2020Quote: ... Sequencing libraries were created using the NEBNext Ultra II DNA Library Prep kit for Illumina sequencing (New England Biolabs E7645L). All cDNA and library clean-up was done using SPRIselect beads (Beckman Coulter B23317).
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bioRxiv - Microbiology 2020Quote: ... double stranded DNA (dsDNA) was generated by use of NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB), purified using PureLink PCR Micro Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from high-titer phage lysates and sequencing libraries were prepared using the Ultra II FS Kit (New England Biolabs) or for ARB14 and ARB25 ...
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bioRxiv - Plant Biology 2021Quote: ... the DNA of the two parents that generated the cross was extracted from flash-frozen young leaves using NEBNext Ultra II kit (NEB) and sequenced to ~40x coverage as 150 bp reads on an Illumina MiSeq at the Biological Nanostructures Lab in the California NanoSystem Institute at UC Santa Barbara ...
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bioRxiv - Plant Biology 2021Quote: ... RNA-Seq libraries were prepared from 1μg high quality RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs), according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2020Quote: ... One microgram of RNA was then converted to cDNA by using random nonamers and ProtoScript II Reverse Transcriptase (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The mRNA libraries were generated using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L, New England Biolabs) and NEBNext Poly(A ...