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Citations for New England Biolabs :
1601 - 1650 of 6017 citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... The latter was prepared using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB, Ipswitch, MA, USA). Briefly ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesized from 500 ng of total RNA using ProtoScript II Reverse Transcriptase according to manufacturer’s instructions (New England Biolabs). SYBR Green I master mix was used for quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We prepared DNA whole genome sequencing libraries using NEBNext UltraTM II DNA Library Prep Kit (Cat. E7645L, NEB Inc.). We quantified DNA on QubitTM 3.0 fluorometer using Qubit High sensitivity dsDNA Assay (#Q32854 ...
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bioRxiv - Molecular Biology 2021Quote: ... libraries were made using the NEBNext Ultra II Directional RNA Library Prep Kit following the polyA workflow (NEB, E7760). A custom protocol was used at Novogene for libraries preparation ...
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bioRxiv - Microbiology 2020Quote: ... regions of ~600 bp containing the sgRNA sequence were amplified using NEBNext Ultra II Q5 Master Mix (NEB #M0544S) in 25 amplification cycles ...
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bioRxiv - Microbiology 2020Quote: ... cDNA libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were generated using NEBNext UltraTM II DNA Library Prep Kit for Illumina (New England Biolabs, MA, USA), and the library quality was assessed on Qubit 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were then prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) according to the manufacture’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Directional cDNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, NEB). The libraries were sequenced using a HiSeq 1500 or 3000 system in single-read mode for 100 or 150 cycles ...
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bioRxiv - Microbiology 2021Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... pET28a-6xHis-MBP-Tev cleavage site-mGFP-FUS-TIS-Strep-tag II was transformed into BL21 E.coli (New England Biolabs). Two fresh colonies were cultivated overnight in 2 × 50 ml SOB medium at 37 °C ...
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bioRxiv - Genomics 2021Quote: ... libraries were generated with New England BioLabs NEBNext® Ultra™ II FS DNA Library Prep (NEB, Ipswich, MA). Samples were sequenced at the Duke Center for Genomic and Computational Biology ...
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bioRxiv - Genomics 2021Quote: Libraries were prepared using an NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs, Inc) with a fragmentation size incubation time of 15 min at 37°C ...
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bioRxiv - Genomics 2021Quote: ... a ChIP-seq library was made using the NEBNext Ultra II DNA Library Prep Kit for Illumina kit (NEB).
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bioRxiv - Molecular Biology 2021Quote: ... NEBNext Ultra II DNA Library Prep Kit for Illumina was used to make the DNA libraries (NEB, #E7645S/L), which were sequenced on the Illumina NovaSeq (PE-150 ...
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bioRxiv - Bioengineering 2020Quote: ... followed by library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Sequencing was performed using a NextSeq 500 (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... RNA passing quality control was converted to a sequencing ready library using the NEBNext Ultra II Directional RNA library kit with polyA selection as per the manufacturer’s instructions (New England Biolabs). The cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for Illumina library quantitation (Kapa Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries for RNA-seq were made with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... then processed for RNA-seq library prep with the NEBNext Ultra II Directional RNA Library Prep Kit (NEB, E7530L), and finally sequenced on an Illumina NextSeq 500 ...
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bioRxiv - Cell Biology 2022Quote: ... The RNA-Seq library was prepared by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced with the NextSeq 500/550 sequencing platform (performed at the NYUAD Sequencing Center) ...
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bioRxiv - Molecular Biology 2022Quote: ... prior to library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) with poly(A ...
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bioRxiv - Molecular Biology 2022Quote: ... ChIP samples were analyzed by qPCR or subjected to library preparation according to the manufacturer’s protocol (NEBNext Ultra II DNA Library Prep Kit for Illumina, NEB) using Dual Index Primers (NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... Library preparation was performed with the NEB Next Ultra II DNA Library Prep Kit for Illumina (NEB, Cat #E7645S) using 25 μL of CUT&RUN DNA as input ...
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bioRxiv - Cell Biology 2022Quote: ... The amplicons (200 fmol) were first end-repaired with the NEBNext Ultra II End repair kit (New England Biolabs), then purified using AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Genomics 2022Quote: ... ChIP samples were processed for library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instruction and sequenced on a Next-Seq 500 Sequencer (Illumina ...
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bioRxiv - Genomics 2022Quote: ... TruSeq-barcoded RNAseq libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Each library was quantified with a Qubit 2.0 (dsDNA HS kit ...
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bioRxiv - Plant Biology 2022Quote: ... RNA Libraries were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced as single end reads using the NextSeq500 platform (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared according to the NEBNext Ultra RNA II directional Library Prep kit (New England Biolabs, MA, USA) after NEBNext rRNA depletion (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... Deglycosylation of purified protein was performed in non-denaturing conditions according to manufacturer’s protocol (Protein Deglycosylation Mix II, NEB).
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bioRxiv - Microbiology 2022Quote: ... Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® was used (New England Biolabs, MA, USA). For sequencing ...
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bioRxiv - Systems Biology 2022Quote: ... PCRs for screens with all other libraries were performed using the NEBNext Ultra II Q5 Master Mix (NEB #M0544L) with 21 to 24 cycles ...
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bioRxiv - Genomics 2022Quote: ... Purified genomic DNA was directly amplified by 22 cycles of PCR using NEBNext Ultra II Q5 PCR MasterMix (NEB). Sequencing was performed on a NovaSeq 6000 (Illumina ...
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bioRxiv - Physiology 2022Quote: ... cDNA was prepared following the NEBNext® Ultra™ II Directional RNA Library Prep Kit protocol (New England Biolabs). The libraries were sequenced using paired-end 150 bp sequencing on a HiSeq 4000 System (Illumina) ...
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bioRxiv - Neuroscience 2022Quote: ... The last two PCR rounds were performed using NEBNext® Ultra™ II Q5® Master Mix (NEB #M0544). Samples were purified using AMPure XP beads (Beckman Coulter #A63880) ...
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bioRxiv - Molecular Biology 2022Quote: ... Bulk mRNA sequencing of triplicate samples was performed using NEBNext® Ultra™ II RNA Library preparation (NEB #E7775) with PolyA selection on the Illumina HiSeq4000 using the standard protocol for Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... ≤500 ng DNase-treated RNA was used to generate cDNA using Protoscript II Reverse Transcriptase (New England Biolabs #M0368) and the standard first-strand cDNA synthesis protocol ...
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bioRxiv - Neuroscience 2022Quote: ... ChIP-Seq libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on a NovaSeq 6000 ...
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bioRxiv - Microbiology 2022Quote: ... The RNA sequencing library was prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using the manufacturer’s instructions (New England Biolabs). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: Sequencing libraries were prepared from 52.5 μg gDNA using two-step PCR strategy with NEBNext Ultra II Q5 Polymerase (NEB). First the gRNA regions were enriched from the genome ...
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bioRxiv - Cancer Biology 2022Quote: ... and ChIP libraries were amplified and barcoded using the NEBNext Ultra II DNA library preparation kit (New England Biolabs) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared using the NEB Next Ultra II DNA library prep kit for Illumina (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: Purified rRic-8A-452 and NCS-1ΔH10/rRic-8A-452 were phosphorylated with casein kinase II (CK2, New England Biolabs) as previously described by McClelland et al ...
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bioRxiv - Molecular Biology 2023Quote: Libraries were prepared for high-throughput sequencing according to manufacturer’s recommendations using the Ultra II DNA Library Prep kit (NEB). Samples were multiplexed and paired-end sequencing was performed using either a MiniSeq or NovaSeq 6000 (Illumina).
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bioRxiv - Microbiology 2022Quote: ... EE62/63AA and the 2_87 double serine phospho-mimetic SE53/57EE or “SS-EE”) using Phusion-II polymerase (New England Biolabs) and standard protocols ...
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bioRxiv - Microbiology 2022Quote: ... A constitutively active version (SS177,181EE) was generated by quick-change site-directed mutagenesis using Phusion-II polymerase (New England Biolabs) and standard protocols ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sheared DNA was then adapter-ligated (AL) with NEBnext Ultra ii Library Preparation kit (New England Biolabs, Ipswich, MA). AL-DNA quality was again confirmed by Bioanalyzer high sensitivity DNA kit (Agilent ...
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bioRxiv - Molecular Biology 2022Quote: ... Sequencing libraries were prepared using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB #E7760) coupled with AMPure XP bead purification (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... Samples were pooled into a library using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) and subsequently sequenced on Illumina NextSeq500 platform (75 bp ...
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bioRxiv - Developmental Biology 2023Quote: ... mRNA Magnetic Isolation Module and the NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina according to the manufacturer’s instructions (New England Biolabs). Libraries were QC-checked on a Bioanalyzer 2100 (Agilent ...