Labshake search
Citations for New England Biolabs :
1501 - 1550 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2022Quote: ... qPCR reactions were conducted using Luna Universal Probe qPCR mastermix (New England Biolabs). Reactions were set up in a total volume of 10 ml containing 0.4 μM of each probe ...
-
bioRxiv - Biochemistry 2022Quote: ... Reactions were stopped by the addition of 1 μL of Proteinase K (NEB) and incubated at room temperature for 15 minutes ...
-
bioRxiv - Biochemistry 2022Quote: ... and resultant DNA was used for ligation reactions with T4 DNA Ligase (NEB). Ligated product was transformed directly into chemically competent NEB5α cells (NEB ...
-
bioRxiv - Molecular Biology 2022Quote: ... each RT reaction was treated with 5000units of RNAse H (New England Biolabs) for 30 minutes at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... The Golden Gate assembly reaction was carried out using BsaI (New England Biolabs) following standard protocols (Lee et al. ...
-
bioRxiv - Molecular Biology 2020Quote: ... 15 µL of labeling mix (4 µL 10X ThermoPol Reaction buffer (NEB, B9004S), 2 µL of 5 mM aa-dUTP/dNTP mix (5 mM each dATP ...
-
bioRxiv - Neuroscience 2021Quote: ... The PCR reaction was DpnI digested (New England Biolabs, 37°C, 1 h) and column purified (Macherey-Nagel ...
-
bioRxiv - Microbiology 2021Quote: ... PCR reactions were performed using Phusion High Fidelity DNA polymerase (New England Biolabs), according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2021Quote: ... The reactions were quenched using 1x purple gel loading dye (New England Biolabs). The reaction products were heated at 95 °C/5 min and separated on 1% agarose gel in Tris/Acetic acid/EDTA (TAE ...
-
bioRxiv - Microbiology 2022Quote: ... All reactions were supplemented with 0.8 U/µL RNase Inhibitor Murine (NEB, M0314S). Purified CapRelSJ46 protein was used at a final concentration of 250 nM ...
-
bioRxiv - Systems Biology 2022Quote: ... with the exception of using the commercial φ29 reaction buffer from NEB (B0269L). Amplification products were cleaned and concentrated using the E.Z.N.A.® Cycle-Pure Kit from Omega BioTek (D6492-02 ...
-
bioRxiv - Genetics 2022Quote: ... Each reaction contained 20 μL and used Q5 polymerase (New England Biolabs, #M0491). The reagents were distributed into each well of a 96 well plate as follows ...
-
bioRxiv - Molecular Biology 2022Quote: ... 3 ng of DNA was used per qPCR reaction (qPCR Master Mix, NEB). PCR was performed using an Applied Biosystems 7500 instrument (software version 2.3) ...
-
bioRxiv - Microbiology 2020Quote: Polymerase chain reaction (PCR) was performed using Q5 DNA polymerase (New England Biolabs). For cloning ...
-
bioRxiv - Synthetic Biology 2020Quote: DNA was pooled and blunt-ended by NEBNext End Repair Reaction Module (NEB), incubating at 20°C for 30 minutes ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and added in equimolar amounts to GGA assembly reactions with BSAI-HF (NEB), T4 ligase (Promega ...
-
bioRxiv - Microbiology 2021Quote: ... The RT reaction was treated with 1 μl RNase H (New England Biolabs) and incubated at 37°C for 20 min to remove RNA ...
-
bioRxiv - Microbiology 2021Quote: ... The reaction mixture was then incubated with 5 units of Antarctic Phosphatase (NEB) for 1 h at 22 °C to hydrolyze unreacted GTP ...
-
bioRxiv - Molecular Biology 2020Quote: ... The ligation reaction was then performed by adding diluted 1.25 μl adaptors (NEB), 15 μl Ligation master mix ...
-
bioRxiv - Genomics 2021Quote: ... Three-primer PCR reactions were performed with OneTaq DNA Polymerase (New England Biolabs) in a 25 µl reaction ...
-
bioRxiv - Genomics 2021Quote: ... the PCR reactions were treated with 200 U/ml of Exonuclease I (NEB) for 1 h at 37°C ...
-
bioRxiv - Genomics 2021Quote: ... the PCR reactions were treated with 200 U/mL of Exonuclease I (NEB) for 1 h at 37°C ...
-
bioRxiv - Biochemistry 2020Quote: ... Reactions were quenched on ice and by addition of 1x Stop Solution (NEB). Immediate isopropanol-based precipitation recovered 75- 95% of input mRNA as mRNA fragments in water.
-
bioRxiv - Physiology 2020Quote: ... Mutagenesis reactions were then digested with Dpn I (New England Biolabs, Ipswich, MA) and transformed into sub-cloning efficiency competent cells (New England Biolabs ...
-
bioRxiv - Microbiology 2021Quote: ... PCR reactions were carried out using Q5 High-Fidelity polymerase (New England Biolabs) and oligomers used for amplifications listed in Table S1 ...
-
bioRxiv - Biochemistry 2021Quote: ... The reaction was stopped by addition of 1 μL proteinase K (NEB, P8107S), followed by 15 min incubation at room temperature and addition of 20% formaldehyde) ...
-
bioRxiv - Biochemistry 2020Quote: ... Low-complexity ssDNA was synthesized in phi29 DNA polymerase reaction buffer (NEB M0269S), 500 μM dCTP and dTTP (NEB N0446S) ...
-
bioRxiv - Genomics 2020Quote: ... The reaction mix included 1 μl of 10x poly(A) polymerase buffer (NEB), 0.1 mM of ATP ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR reaction was performed with Q5 High-Fidelity Polymerase Master Mix (NEB) and the product column purified with a QIAquick PCR purification kit (Qiagen ...
-
bioRxiv - Molecular Biology 2020Quote: ... the 12.5 μl reaction mixture contained 1X OneTaq Hot Start Master Mix (NEB), 0.2 μM of each DHPV-U 1622F and DHPV-U 1887R primers and l μl of PCR product from the first-step PCR reaction ...
-
bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were performed with Taq 2X Master Mix (New England BioLabs, M0270L). The thermocycler conditions for Mouse Chk1 and GAPDH PCR product were as follows ...
-
Glucocorticoid receptor collaborates with pioneer factors and AP-1 to execute genome-wide regulationbioRxiv - Genomics 2021Quote: ... 1.35 μL MNase (New England Biolabs, diluted 1:10 in MNase reaction buffer) was added to the resuspended chromatin and incubated at 37°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... The reactions were further processed and digested with P1 Nuclease (Cat#: M0660S, NEB). In vitro-transcribed 4B mRNA (firefly luciferase reporter (Bukhari et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... The PCR reaction was performed using Phusion High-Fidelity DNA Polymerase (NEB #M0530) with 10 µM of each primer (synthesized by IDT ...
-
bioRxiv - Synthetic Biology 2023Quote: ... In the second Type IIS DNA assembly reaction using BbsI (New England Biolabs), these transcriptional unit constructs were assembled into the backbone plasmid pML3001 (Lebovich and Andrews 2022 ...
-
bioRxiv - Biophysics 2023Quote: ... 163 and 71 using a BsaI-HFv2 golden gate reaction mix (NEB E1601). The intermediate vectors were made using traditional cloning techniques (in details described in Table S1).
-
bioRxiv - Molecular Biology 2023Quote: ... Reaction was set up using ARCA (NEB, S1411L or Trilink, N-7003-5) and N1-Methylpseudouridine-5’-Triphosphate (Trilink ...
-
bioRxiv - Neuroscience 2023Quote: ... In-Fusion reactions were transformed into NEB Stable competent cells (New England Biolabs). Insert-containing clones were subjected to restriction digest analysis and sequence verification prior to subsequent cloning steps or packaging into viral particles ...
-
bioRxiv - Microbiology 2023Quote: ... The PCR reactions were performed as recommended by the manufacturer (New England Biolabs) with optimized annealing temperatures as indicated in Supplementary Table 2 ...
-
bioRxiv - Biophysics 2023Quote: ... An in vitro translation reaction was then assembled using the PURExpress system (NEB) with the following ...
-
bioRxiv - Microbiology 2023Quote: ... and reactions were carried out in accordance with the manufacturer’s instructions (NEB, M5510A) to generate new plasmids ...
-
bioRxiv - Biophysics 2023Quote: The polymerization reactions were performed with Bst 2.0 DNA Polymerase (New England BioLabs). The conditions were according to the protocol provided by the manufacturer ...
-
bioRxiv - Cancer Biology 2023Quote: ... The reaction was transformed into chemically competent NEB Stable cells (NEB Cat#C3040H) and the plasmid was verified by restriction enzyme digestion and Sanger sequencing ...
-
bioRxiv - Biochemistry 2024Quote: ... For the in-vitro transcription and translation reactions in PURExpress (New England Biolabs), nuclease PCR products were added at 5 nM final concentration and expressed at 37°C for 2 hours ...
-
bioRxiv - Bioengineering 2024Quote: ... Transcription reactions were purified using the Monarch RNA cleanup kit (New England Biolabs). mRNA concentration was quantified by nanodrop spectrophotometer ...
-
bioRxiv - Biochemistry 2024Quote: ... reactions were quenched on ice with STOP Buffer (0.4 U proteinase K (NEB), 18 mM EDTA ...
-
bioRxiv - Microbiology 2023Quote: ... and then an assembly reaction using the NEBuilder HiFi DNA Assembly Kit (NEB) was performed to insert the Ultramers into the linearized vector and re-circularize ...
-
Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... All PCR reactions were performed using the Q5 High-Fidelity DNA Polymerase (NEB) kit following the instructions enclosed ...
-
bioRxiv - Microbiology 2024Quote: ... a ligation reaction was conducted using the T4 DNA ligase (New England Biolabs) with a vector-to-insert ratio of 1:3 ...
-
bioRxiv - Immunology 2024Quote: ... Second round nested-PCR reactions were performed using Phusion proof reading polymerase (NEB). A third round of PCR was performed with HotStar Taq DNA polymerase master mix (Qiagen ...