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Citations for New England Biolabs :
1401 - 1450 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... We mixed 43μL of the second step PCR product with 2μL of ApaLI (NEB #R0507L) and 5μL of 10X Cutsmart and incubated at 37°C for at least 2 hours (up to overnight) ...
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bioRxiv - Immunology 2020Quote: ... The cDNA product was end-repaired using the NEBNext End Repair Module (New England Biolabs). RNA-Seq libraries were prepared using 1 μg of end-repaired cDNA using the TruSeq Stranded RNA Kit (Illumina) ...
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bioRxiv - Genetics 2021Quote: DJ-PCR products were produced with Q5® High-Fidelity DNA Polymerase (New England Biolabs) via the method of Yu et al ...
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bioRxiv - Cell Biology 2021Quote: ... Each PCR product was amplified using EpiMark Hot Start Taq DNA Polymerase (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Both PCR products were then assembled using NEBuilder® HiFi DNA Assembly Master Mix (NEB), resulting in VHp693.
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bioRxiv - Cell Biology 2020Quote: ... a pair of overlapping PCR products were generated with Phusion high fidelity DNA polymerase (NEB) using pUAS-Abl:GFP (Fox and Peifer 2007 ...
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bioRxiv - Genetics 2021Quote: ... PCR products spanning the gRNA-targeted site were digested with BfaI (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified products were ligated using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA). The ligated product was transformed into Escherichia coli (E ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA products were purified on gel and amplified by PCR using the Phusion polymerase (NEB). Libraries were sequenced at the iGE3 Genomic Platform (UNIGE ...
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bioRxiv - Immunology 2020Quote: ... The obtained bead-bound cDNA product then underwent Exonuclease I treatment (New England Biolabs; M0293M) to remove excess primer before proceeding with second strand synthesis.
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bioRxiv - Cell Biology 2021Quote: ... PCR products were used directly as templates for IVT using HiScribe T7 transcription kit (NEB). Resulting RNA was purified using RNAClean XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... and Phusion DNA polymerase after 20 cycles of amplification (1.2×10−5) (NEB, product literature). Because the error rate from the RNA polymerase was much higher than the RT and PCR steps ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were purified using a Monarch PCR & DNA Cleanup Kit (5 μg) from NEB and then visualized on an 0.8 to 1.2 % percent agarose gel resolved in Tris-acetate-EDTA electrophoresis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... PCR products were purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and submitted to the Massachusetts General Hospital DNA Core ...
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bioRxiv - Cell Biology 2022Quote: ... gRNA was phosphorylated by a T4 polynucleotide kinase according to product instructions (New England Biolabs). For the amplification of phosphorylated sgRNA it was incubated with 1 μl of forward and reverse primers (100 μM ...
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bioRxiv - Genomics 2022Quote: ... The PCR products were used as the template for in vitro transcription (NEB, Cat. E2040S) followed by reverse transcription (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... We used the product of Gibson assembly for transformation into DH5α competent cells (NEB: C2987H). Transformed cells were cultured directly in LB liquid ...
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bioRxiv - Immunology 2022Quote: ... Both the PCR product and the recipient plasmid were digested with XhoI and BamHI (NEB) according to manufacturer’s protocols ...
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bioRxiv - Genetics 2022Quote: ... PCR product sizes were determined by comparison with 50 bp DNA ladder (New England Biolabs) using ImageLabTM software (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... the PCR product was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (E2040S; NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... PCR product sizes were determined by comparison with 50 bp DNA ladder (New England Biolabs) using ImageLab™ software (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the desired product was agarose gel purified using a Monarch gel extraction kit (NEB) and sequenced on an Illumina MiSeq.
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bioRxiv - Biochemistry 2024Quote: ... Purified products were again transformed into either NEB 10-beta (New England Biolabs, Ipswich, MA) or SIG10 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... The purified PCR product was digested by KpnI and XhoI (NEB, underlined in primer sequences) and repurified ...
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bioRxiv - Physiology 2024Quote: ... After the PCR 20ul of the PCR product were restriction digested with BamH1 (NEB #xxx) for 1 hr at 37C ...
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bioRxiv - Genomics 2024Quote: Libraries were prepared using the NEBNext DNA Modules Products (New England Biolabs, Ipswich, MA, USA) with an ‘on bead’ protocol developed by Genoscope ...
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bioRxiv - Molecular Biology 2024Quote: ... Robust synthesis of expected RNA product was comparable to commercially available T7 RNA polymerase (NEB).
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bioRxiv - Molecular Biology 2024Quote: ... Annealed products were ligated to the cleaved px330 plasmid using T4 DNA ligase (NEB, M0202T) and specific buffer (NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were purified with enzymes ExoI and rSAP (New England Biolabs, Ipswich, MA, USA), Sanger sequenced (Macrogen ...
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bioRxiv - Microbiology 2024Quote: ... PCR products and the respective antibiotic resistance gene were used for Gibson assembly (NEB, USA) (38) ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified using Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA). DNA concentration was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and PCR products were assembled using the NEBuilder HiFi DNA Assembly kit (New England Biolabs) to generate the final construct pET788 ...
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bioRxiv - Immunology 2024Quote: ... Purified round 2 PCR products were cloned using the NEB PCR Cloning Kit (NEB, #E1202) and sequenced with Sanger sequencing.
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bioRxiv - Biochemistry 2024Quote: ... and the PCR products were subsequently 5’ phosphorylated using T4 polynucleotide kinase (New England Biolabs) and self-ligated by T4 DNA ligase (New England Biolabs ...
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bioRxiv - Bioengineering 2024Quote: ... The resultant PCR product was reassembled into a tdTomato-containing vector via Gibson assembly (NEB) with a tdTomato insert (sequence ordered as a codon-optimized gBlock from IDT ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was mixed with the vector fragment and assembled with HiFi assembly (NEB). This strategy maintains variant barcode matching from residues for the library up until L290 ...
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bioRxiv - Microbiology 2022Quote: ... All PCR products were gel purified by using the Monarch DNA Gel Extraction Kit (NEB) and eluted in 20 μL nuclease-free water ...
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bioRxiv - Molecular Biology 2023Quote: ... and the desired products were agarose gel purified using a Monarch gel extraction kit (NEB) and sequenced on an Illumina HiSeq.
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bioRxiv - Developmental Biology 2023Quote: ... The protein products were purified using the NEBExpress Ni Spin Columns (New England Biolabs, S1427S). The concentration of the purified products was quantified using the Qubit protein assay kit (Thermo Fisher ...
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bioRxiv - Genomics 2023Quote: ... The purified products were digested with Lambda Exonuclease (0.5U/ul) in 1x buffer (NEB M0262L) for 2 hours and purified with Zymo ssDNA/RNA clean & concentrator kit ...
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bioRxiv - Cell Biology 2023Quote: ... Purified PCR products and pAcGFP1-N1 were incubated with XhoI and BamHI (New England Biolabs) under 37 °C for 4 hrs in 1X buffer 3 supplemented with 1% BSA ...
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bioRxiv - Genetics 2023Quote: ... PCR products were visualized on 1% agarose gels with a 1 kb plus ladder (NEB).
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bioRxiv - Developmental Biology 2023Quote: ... Homology arms (PCR products from genomic DNA) were inserted with Gibson assembly (New England Biolabs) into pDD287 (Addgene #70685) ...
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bioRxiv - Genomics 2022Quote: ... Original vector backbone and intermediate backbone product were designed for digestion with Esp3I (BsmBI, NEB), inserts were designed for digestion with BsaI (NEB).
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bioRxiv - Plant Biology 2023Quote: ... The ligation product was introduced into NEB 5α competent Escherichia coli cells (NEB, Evry, France), transformants selected on kanamycin (50 µg.mL-1 ...
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bioRxiv - Biophysics 2023Quote: ... KODextreme polymerase was used for amplification and the product was ligated (KLD enzyme mix, NEB) and transformed into NEB® 5-alpha Competent cells ...
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bioRxiv - Cell Biology 2023Quote: ... and mutant Pacer RH domain PCR product were mixed with Gibson Assembly master mix (NEB) in a 1:3:3 molar ratio prior to incubation at 50 °C for 15 minutes and transformation into XL10 E ...
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bioRxiv - Immunology 2023Quote: ... IVT products were purified with Monarch RNA purification columns (NEW ENGLAND BioLabs Inc. MA, USA) and resuspended in a TE buffer at a desired concentration ...
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bioRxiv - Genomics 2023Quote: ... The PCR product was gel purified using the Monarch DNA Gel Extraction Kit (NEB T2010) and used for IVT reaction using the NEB HiScribe T7 ARCA mRNA Kit (E2065S).
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bioRxiv - Cell Biology 2023Quote: ... Subsequent PCR product and pEF.CMV.RFP was digested with EcoRV according to the manufacturer’s protocol (NEB). These were then ligated together using T4 ligase (NEB ...