Labshake search
Citations for New England Biolabs :
1751 - 1800 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... All PCR reactions were performed using Phusion High-Fidelity DNA Polymerase (New England Biolabs). MISSION shRNA constructs were obtained from Sigma in pLKO.1-puro vectors ...
-
bioRxiv - Synthetic Biology 2021Quote: Total RNA was extracted from cell-free expression reactions with a kit (NEB #T2010), following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... and 10 pmol of each used in a 4-fragment Gibson assembly reaction (NEB) to generate GT10 5’_pSY45_pDS66_GT10cHA3 Flox_GT10 3’_pUC19 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... PCR reactions were conducted using Taq DNA Polymerase (New England BioLabs, Ipswich, MA, USA) with the primers listed in supplementary table S1 ...
-
bioRxiv - Genetics 2021Quote: ... All PCR reactions were performed using Q5 High-Fidelity DNA polymerase (New England Biolabs).
-
bioRxiv - Genetics 2021Quote: ... All components were combined using a Gibson assembly reaction (New England Biolabs ref # E2611L) following the manufacturer’s protocol and transformed into XL1 blue competent cells ...
-
bioRxiv - Molecular Biology 2021Quote: ... The reaction was performed at 37° C in Smart buffer (New England Biolabs, USA) in a Real-time PCR machine (Rotor-Gene Q ...
-
bioRxiv - Immunology 2021Quote: ... Second PCR reactions were carried out using Phusion® High-Fidelity DNA Polymerase (NEB) with the following cycling conditions ...
-
bioRxiv - Genomics 2020Quote: ... and 1µl cDNA in 25µL reactions with One Taq DNA polymerase (New England Biolabs). Amplicons were examined under UV light on 1% agarose gels stained with ethidium bromide ...
-
bioRxiv - Molecular Biology 2023Quote: ... The reaction with T4 RNA ligase 2 was performed in 1x T4 Rnl2 (NEB) supplemented with PEG 8000 (10% ...
-
bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed using Phusion 5X Master mix (New England Biolabs GmbH, M0492S). For a 25 µl reaction ...
-
bioRxiv - Biophysics 2023Quote: ... For this we performed two subsequent KLD cloning reactions (M0554, New England Biolabs, UK). In the first reaction we made a PCR-fragment with Q5 DNA polymerase (M0491 ...
-
bioRxiv - Immunology 2023Quote: ... GoldenGate reaction was run according to NEBridge protocol and transformed into NEB10β cells (NEB), and recovered in 50mL of TB media with Carbenicillin overnight at 37°C shaking incubator ...
-
bioRxiv - Genetics 2023Quote: ... Reactions were performed using the Luna Universal One-Step RT-qPCR Kit (NEB, E3005L) and a CFX Opus 384 Real-Time PCR System (Bio-Rad).
-
bioRxiv - Molecular Biology 2023Quote: ... The reverse-transcription (RT) reactions were performed with 2X LunaScript RT SuperMix Kit (NEB) with 5 μL of RNA input (250 ng of RNA total ...
-
Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... These reactions were cleaned using the NEB Monarch RNA Cleanup protocol (NEB, Cat# T2040L) and used as anti-sense RNA probes for fluorescent in situ hybridization.
-
bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed utilizing the Phusion High-Fidelity PCR Master Mix (NEB, M0531L) to prevent any mutations ...
-
bioRxiv - Genomics 2023Quote: ... and resuspended in MNase reaction with 1mM CaCl2 and 25U/μL MNase (NEB, M0247S). The reaction was incubated at 16°C for 12min ...
-
bioRxiv - Microbiology 2023Quote: ... used for Polymerase Chain Reaction (PCR) and Golden gate cloning kit (New England Biolabs) were carried out according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... The restriction and ligation reactions were done using BsaI-HF and T4 ligase (NEB), respectively ...
-
bioRxiv - Bioengineering 2023Quote: ... After a 30 cycles PCR reaction with Q5 hot-start high-fidelity polymerase (NEB) following recommended vendor protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... and resuspended in 1X DNase I reaction buffer with 1 µL DNase I (NEB) for 15 minutes at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... Secondary amplification reactions were performed using NEBNext high-fidelity 2 PCR master mix (NEB) in 50 μl reactions (26 μl of 2X mix ...
-
bioRxiv - Cell Biology 2022Quote: ... All PCR reactions were carried out with Q5 High-Fidelity DNA Polymerase (NEB #M0491). The cDNA of Pkd2 was amplified from the plasmid Pkd2-EGFP-N1 (Lab stock ...
-
bioRxiv - Cell Biology 2022Quote: ... were amplified by polymerase chain reaction (PCR) using Q5 High-Fidelity DNA polymerase (NEB) and digested using NotI ...
-
bioRxiv - Biophysics 2022Quote: ... diluted to ∼1 nM in 1x T4 DNA ligase reaction buffer (New England Biolabs), and treated with T4 DNA ligase to circularize the DNA ...
-
bioRxiv - Synthetic Biology 2024Quote: ... while Gibson assembly reactions used the NEBuilder(R) HiFi DNA Assembly Cloning Kit (NEB). Transformed bacteria were grown for 24h at 32°C ...
-
bioRxiv - Bioengineering 2024Quote: ... qPCR reactions were set up using the Luna Universal qPCR Master Mix (NEB M3003S). Reactions included 6 µL of Luna Mastermix ...
-
bioRxiv - Bioengineering 2024Quote: ... All A-packaged effector plasmids were generated using an isothermal assembly reaction (NEB M5520AA) (13).
-
bioRxiv - Molecular Biology 2023Quote: ... Reactions were performed as technical triplicates using Luna® Universal qPCR Master Mix (NEB). Primers listed in Tab S9 were used for amplification of mitochondrial cob ...
-
bioRxiv - Molecular Biology 2024Quote: ... 20 µL of reactions were treated with 2 µL DNase I (NEB, Cat # M0303S) in a total volume of 100 µL at 37°C for ∼ 20 min before further analysis and treatment.
-
bioRxiv - Systems Biology 2023Quote: ... The reverse transcription reaction mix (1x RevertAid RT buffer, 250 μM dNTPs (NEB N0447L), 0.2 mg/mL recombinant albumin (NEB B9200S) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 10 pmol of each used in a 4-fragment Gibson assembly reaction (NEB) to generate TbGT8 5’_BSDr_TbGT8 3’_pUC19 ...
-
bioRxiv - Genomics 2024Quote: ... Two 50 μl PCR reactions were performed per sample with Q5 Polymerase (NEB M0492S) with real-time tracking using SYBR green dye with the following cycling parameters 98°C - 3 min ...
-
bioRxiv - Genomics 2024Quote: ... Four 50 μl PCR reactions were performed per sample with Q5 Polymerase (NEB M0492S) with the following cycling parameters ...
-
bioRxiv - Biochemistry 2024Quote: ... PCR reactions were performed using Q5 High-Fidelity DNA polymerase (New England Biolabs, USA) and purified using DNA Clean and Concentrator 5 kit (Zymo) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... PCR reactions contained 1X Phusion High-Fidelity PCR Master Mix (New England BioLabs Inc.), 0.5 μM primers (forward ...
-
bioRxiv - Molecular Biology 2023Quote: ... in a reaction mix containing 1× T4 RNA Ligase Buffer (New England Biolabs, M0437M), 1U SUPERase•In RNase inhibitor (Invitrogen ...
-
bioRxiv - Immunology 2024Quote: ... Amplification reactions consisted of 50 μL LongAmp Taq 2x Master Mix (New England Biolabs), 1 μL 10 μM forward primer ...
-
bioRxiv - Synthetic Biology 2024Quote: ... the backbone PCR reaction was digested overnight with DpnI (New England Biolabs, Ipswich, MA) at 37°C ...
-
bioRxiv - Genomics 2023Quote: ... The 5′ UTR and coding sequence were amplified in Q5 polymerase reactions (NEB, M0491) with 10 ng HsCD00617865 template and 500 nM forward and reverse primers for 25 cycles using manufacturer’s recommendations with 55 °C annealing temperature ...
-
bioRxiv - Microbiology 2023Quote: ... The qPCR reaction was performed with Taq DNA polymerase (New England Biolabs, Beverly, MA) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Polymerase chain reaction (PCR) was performed with Q5-HF polymerase (New England Biolabs, USA) or with CloneAmp Hifi polymerase (Takara Bio ...
-
bioRxiv - Biochemistry 2023Quote: ... The reactions were adjusted to 80 μL containing 1x NEBuffer r1.1 (New England Biolabs) and a final concentration of 1.4 nM of hRNase 4 and 0.15 U/μL of polynucleotide kinase (New England Biolabs) ...
-
bioRxiv - Biophysics 2023Quote: ... Capping reactions were then purified using Monarch RNA clean-up columns (New England Biolabs).
-
bioRxiv - Synthetic Biology 2023Quote: Polymerase Chain Reaction (PCR) was performed using Q5 High Fidelity 2X Master Mix (NEB) with primers synthesized by Integrated DNA Technologies (IDT ...
-
bioRxiv - Microbiology 2023Quote: ... the PCR reaction was purified using the Monarch PCR Cleanup Kit (New England Biolabs). DNA was sent for Sanger sequencing ...
-
bioRxiv - Biochemistry 2023Quote: ... reactions were performed with mutagenesis primers and the Q5 DNA polymerase (New England Biolabs). Afterward ...
-
bioRxiv - Cell Biology 2023Quote: ... All PCR reactions were performed using the Phusion high fidelity DNA polymerase (NEB, M0530S). All the PCR-amplified fusion genes and epitope tags were cloned into the pSKP32 vector (Kaeser-Pebernard et al. ...
-
bioRxiv - Microbiology 2023Quote: ... along with NEBNext Quick Ligation Reaction Buffer and T4 DNA ligase (both from NEB). The reaction incubated for 10 minutes at room temperature ...