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Citations for New England Biolabs :
1301 - 1350 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The reaction mixture contains Luna Universal qPCR Master Mix (New England Biolabs), 200 nM each of the primers ...
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bioRxiv - Microbiology 2024Quote: All PCR reactions were performed using Phusion HF polymerase (New England Biolabs). All oligonucleotides were purchased from Integrated DNA Technologies (IDT) ...
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bioRxiv - Developmental Biology 2024Quote: ... A primer extension reaction was performed using Phusion DNA polymerase (M0530L, NEB) followed by QIAquick purification (28106 ...
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bioRxiv - Genomics 2024Quote: ... then ligated to reporter gene inserts in multiple reactions (NEB T4 ligase). Ligation products were transformed into electrocompetent cells and grown in liquid culture from which we prepared plasmid DNA.
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bioRxiv - Evolutionary Biology 2023Quote: ... using 1/8 reaction of NEBNext dsDNA Fragmentase (#M0348, New England Biolabs) incubated at 37°C for 5 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... and reaction samples were loaded onto 1 ml of amylose resin (NEB). Proteins in the flow-through fractions were further purified by gel filtration chromatography (Superdex 200pg ...
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bioRxiv - Molecular Biology 2024Quote: ... Q5® High-Fidelity DNA Polymerase and its recommended reaction buffer (NEB) were utilized for the amplification process.
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Constitutive and conditional epitope-tagging of endogenous G protein coupled receptors in DrosophilabioRxiv - Neuroscience 2023Quote: ... and combined in an assembly reaction (NEB, Ipswitch, MA, Cat. No. 2621) to create a donor vector for genomic modification ...
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bioRxiv - Immunology 2024Quote: ... TAP-PCR reaction was performed using 5 μL of Q5 polymerase (NEB), 5 μL of GC Enhancer (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... a NEBNext Quick Ligation Reaction Buffer (New England Biolabs, cat. no. B6058) and a NEBNext Companion Module for Oxford Nanopore Technologies (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... and RNase H in NEBNext® Second Strand Synthesis Reaction Buffer (NEB) at 16 ºC for three hours ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Ligation reactions were performed using T4 DNA ligase (New England Biolabs, M0202S) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... TET2 reaction was terminated by addition of 0.6 μL stop solution (NEB) and incubation at 37°C for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reactions were mixed with Gel Loading Dye Blue (New England BioLabs Inc.) and ran on a 2% agarose gel with 1X SYBR Safe DNA gel stain (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... The reaction system was prepared according to the kit’s requirements (Yugong Biolabs) and fluorescence quantitative analysis was performed using the ABI 7500 PCR system (Applied Biosystems) ...
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bioRxiv - Synthetic Biology 2024Quote: ... were used with 1 µL T4 DNA Ligase Reaction Buffer (NEB, 10x). We found that using these enzymes resulted in higher efficiency and fidelity for the cloning of mismatched gRNA libraries ...
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bioRxiv - Molecular Biology 2023Quote: ... All assembly reactions were done using NEBuilder HiFi DNA assembly mix (NEB). The resulting plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... All assembly reactions were done using NEBuilder HiFi DNA assembly mix (NEB). The constructs were digested with XhoI and introduced into the BTU1 locus of the new MAC of conjugating two SPT6 KO homozygous heterokaryon strains at 10 hpm by electroporation ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were purified using the Monarch PCR Purification Kit (NEB # T1030L) and eluted in 10µl elution buffer.
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bioRxiv - Microbiology 2023Quote: ... cDNA reactions were purified using the Monarch PCR Purification Kit (NEB #T1030L) and eluted in 10µl elution buffer ...
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bioRxiv - Microbiology 2023Quote: ... All PCR reactions were conducted using the Q5 polymerase (New England BioLabs) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... the reactions were treated with 1 μl of DpnI (New England BioLabs) for 1 hour at 37 °C before gel purification ...
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bioRxiv - Bioengineering 2023Quote: ... the reactions were treated with 1 μl of DpnI (New England BioLabs) for 1 hour at 37 °C before gel purification ...
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bioRxiv - Cancer Biology 2023Quote: ... All PCR reactions were done with Q5 high-fidelity DNA polymerase (NEB), and PCR products were subsequently purified with DNA clean-up after each PCR and restriction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Ligation reactions were performed using T4 DNA ligase (New England Biolabs, M0202S) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The Assembly reactions were transformed into DH-10b electro-competent cell (NEB) and split into 2% and 98% before spreading on Agar plates containing 50ng/ul Kanamycin ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR was carried out in 50 μl reactions using Q5 polymerase (NEB) according to manufacturer’s recommendations with 1 ng of the RGR plasmid as template and 500 nM of the following primers (lower case indicating nucleotides added for cloning purposes and restriction site underlined) ...
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bioRxiv - Biochemistry 2022Quote: ... purified cDNA was added to an NEBNext second-strand synthesis reaction (NEB) at 16 °C for 150 min ...
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bioRxiv - Cancer Biology 2022Quote: ... 1x ThermoPol reaction buffer (New England Biolabs; referred to as FEN1 buffer); and the mixed buffer reactions contained final concentrations of 1x CutSmart buffer (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... This reaction was performed for 22 cycles using Q5 (New England Biolabs). These Illumina sequences were then used as the template for a second of PCR to add indexes and the p5 and p7 Illumina adapter sequence to make finished sequencing libraries ...
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bioRxiv - Developmental Biology 2022Quote: ... mRNA synthesis reactions were cleaned using Monarch RNA Cleanup Kit (NEB: #T2040L) and visualized using agarose gel-electrophoresis ...
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bioRxiv - Cell Biology 2022Quote: ... All PTet plasmids were generated using Gibson reactions (NEB HiFi Assembly Mix) from PCR products ...
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bioRxiv - Microbiology 2022Quote: ... T5 exonuclease diluted 1:5 with 5X reaction buffer (New England BioLabs) (0.01 units/µL) ...
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bioRxiv - Microbiology 2022Quote: ... Six PCR reactions using the Q5® High-Fidelity DNA Polymerase (NEB) were performed to replace WU-Fragment 9 and 10 to create the SARS-CoV-2S-Delta ...
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bioRxiv - Microbiology 2022Quote: ... Six PCR reactions using the Q5® High-Fidelity DNA Polymerase (NEB) were performed.
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bioRxiv - Cell Biology 2022Quote: ... and Illumina adaptor ligation mix [0.075x End repair reaction buffer (E6050, NEB), 0.32× Ligation master mix (E7595 ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was stopped by adding 6x Purple DNA-Loading dye (NEB) and boiled at 90°C for 5min ...
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bioRxiv - Molecular Biology 2023Quote: ... then resuspended in 1X T4 PNK Reaction Buffer (New England Biolabs B0201). Samples were incubated at 37°C for 1 h after the addition of T4 PNK (New England Biolabs M0201 ...
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bioRxiv - Microbiology 2022Quote: ... 20 μL NEBNext 5× Quick Ligation Reaction Buffer (New England Biolabs, UK), and 10 μL of Quick T4 DNA Ligase ...
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bioRxiv - Molecular Biology 2023Quote: ... All PCR reactions were done using Q5 high-fidelity polymerases (M0493S, NEB), and all constructions and intermediates were generated using a NEBuilder HiFi DNA assembly cloning kit (E5520S ...
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bioRxiv - Genomics 2023Quote: ... The inactivated reactions were purified with the Monarch PCR purification kit (NEB).
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bioRxiv - Cell Biology 2023Quote: ... Kinase reactions were performed with 1 μL commercial CK1δ (New England Biolabs) in 1x PMP buffer supplemented with 100 μM unlabeled ATP ...
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bioRxiv - Microbiology 2023Quote: ... T5 exonuclease diluted 1:5 with 5X reaction buffer (New England BioLabs) (0.01 units/μL) ...
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bioRxiv - Biophysics 2023Quote: ... Reactions were stopped by adding 0.8 units of Proteinase K (NEB # P8107S) followed by incubation for 10 min at 37°C ...
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bioRxiv - Genetics 2024Quote: ... PCR reactions were performed with Q5 High-Fidelity DNA Polymerase (NEB, UK). Genomic integration of both constructs were confirmed through PCR and Sanger sequencing ...
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bioRxiv - Cell Biology 2024Quote: For PCR reaction: Taq 2x Master Mix (New England Biolabs Inc, M0270L).
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bioRxiv - Synthetic Biology 2024Quote: ... 50uL reactions were prepared using 1x Q5 Hotstart Master mix (NEB M0494S), 0.5uM Forward primer ...
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bioRxiv - Biochemistry 2024Quote: The reactions were stopped by adding 1 µL of Proteinase K (NEB) to the sample and incubation at 37°C for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... each reaction included 1X Phusion High-Fidelity master mix (New England Biolabs), 200 nM primers ...
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bioRxiv - Microbiology 2024Quote: ... We performed polymerase chain reactions using Q5 high-fidelity DNA polymerase (NEB). Chemical ingredients to make buffers and growth media were purchased from Sigma or VWR.