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Citations for New England Biolabs :
1251 - 1300 of 2894 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... coli BL-21 (DE3) (New England Biolabs) cells and 5mL starter cultures were grown overnight at 37C in Lysogeny Broth (LB ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli XL-1 Blue (New England Biolabs) and all sequences were confirmed by colony PCR and whole plasmid sequencing (Primordium Labs ...
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bioRxiv - Microbiology 2023Quote: ... coli C2566 pRARE2 cells (New England Biolabs). Proteins were expressed overnight at 17°C in TB (with 25 µg/mL Kanamycin and 17 µg/mL Chloramphenicol) ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21(DE3) pLysY (New England Biolabs) were transformed with the various plasmids ...
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bioRxiv - Microbiology 2023Quote: ... coli strain BL21(DE3) (New England Biolabs) grown in either LB media ...
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bioRxiv - Molecular Biology 2023Quote: ... coli T7 Express strain (New England Biolabs) as described33.
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bioRxiv - Developmental Biology 2023Quote: ... coli DNA polymerase I (NEB, cat# M0209S), 50μM dATP ...
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bioRxiv - Biophysics 2023Quote: ... coli DH5alpha cells (New England Biolabs, C2987I). Transformants were grown to saturation in 5-8mL of LB media supplemented with ampicillin (100µg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... coli T7 shuffle cells (New England Biolabs) after induction of protein expression by addition of isopropyl-β-D-thiogalacto-pyranoside (IPTG ...
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bioRxiv - Molecular Biology 2023Quote: ... coli poly(A) polymerase (New England Biolabs). The final GFP mRNA was purified with the Monarch RNA purification kit.
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bioRxiv - Cell Biology 2023Quote: ... coli SHuffle cells (New England Biolabs, C3026J) for disulfide bond formation and higher stability (Lobstein et al. ...
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bioRxiv - Biochemistry 2023Quote: Escherichia coli BL21(DE3) cells (NEB, USA) were transformed with pET-21b plasmid encoding for N-terminally His-tagged NanoLuc gene ...
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bioRxiv - Genetics 2024Quote: ... coli 10β cells (New England BioLabs, C3019I). Following heat-shock ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strain NEB10β (New England Biolabs, MA) was used for all cloning experiments ...
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bioRxiv - Cell Biology 2024Quote: ... coli BL21 (DE3) (C2527I, New England Biolabs) and grown in 1 L of Terrific Broth (TB ...
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bioRxiv - Biophysics 2024Quote: ... coli BL21(DE3)T1R (New England Biolabs) was used to express full-length NadA variant 3 following transformation with pET21-NadA ...
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bioRxiv - Biophysics 2024Quote: ... coli SHuffle® T7 (New England Biolabs) as described (63) ...
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bioRxiv - Biophysics 2023Quote: Escherichia coli BL21 (DE3) cells (#C2527, NEB) were transformed with the appropriate plasmid and grown at 37°C to an OD600 of 0.8 - 1.0 in MOPS-buffered Terrific broth (TB) ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 NICO DE3 (New England Biolabs) chemically competent cells for expression ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL-21 cells (New England Biolabs) were used for the transformation of pET-28b(+ ...
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bioRxiv - Biochemistry 2024Quote: ... coli NEB C2566 cells (New England Biolabs). The cells were grown at 37°C in TB medium for HINT1 ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) cells (New England BioLabs).
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bioRxiv - Biophysics 2024Quote: ... coli chemically-competent cells (New England BioLabs). Single colonies of transformed cells from agar plates containing 100 μg/mL ampicillin and grown at 37 °C were used for starter cultures using 50 mL LB media in 250 mL shake flasks containing 100 μg/mL ampicillin ...
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bioRxiv - Cell Biology 2024Quote: ... coli Poly(A) Polymerase (New England Biolabs), following the manufacturers’ protocol ...
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bioRxiv - Bioengineering 2023Quote: ... coli BL21 (DE3) (New England Biolabs C2527H) was transformed with pRSET B-GA-MatryoshCaMP6s ...
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bioRxiv - Microbiology 2024Quote: ... coli DNA polymerase I (NEB, cat.m0209, USA), RNase H (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... coli inorganic PPase (New England Biolabs, Inc). All reagents were purchased from Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... coli (High Efficiency) (New England Biolabs C2984H) and purified using the NucleoBond Xtra Maxi EF kit (Machery Nagel 740424).
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bioRxiv - Microbiology 2023Quote: ... was amplified from JE2 using the primer pair 3810/9647 and the snap-tag coding sequence amplified from pSNAP-tag (T7)-2 (New England Biolabs, NEB; Supplementary Information) using the primer pair 9631/9632 ...
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bioRxiv - Zoology 2020Quote: ... Moloney murine leukemia virus reverse transcriptase (New England Biolabs, Evry, France) and random hexamer primers were used to transcribe 1 μg RNA in a 20 μl reaction into cDNA.
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bioRxiv - Developmental Biology 2021Quote: ... All oligos were radioactively capped using Vaccinia virus capping system (NEB) and [α-32P]-GTP (Perkin-Elmer) ...
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bioRxiv - Molecular Biology 2020Quote: ... with mCherry-tag by Gibson Assembly (New England Biolabs).
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bioRxiv - Biophysics 2022Quote: ... and 0.12 µM SiR-SNAP-Tag ligand (NEB, S9102S) for 30 min at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit polyclonal anti-SNAP-tag (New England BioLabs, P9310S), mouse monoclonal anti-HSP90 (BD Transduction Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... anti-SNAP-tag antibody (New England Biolabs, Ref. P9310S), anti-clathrin heavy chain antibody (BD Bioscience ...
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bioRxiv - Microbiology 2023Quote: ... was amplified from JE2 using the primer pair 3810/9647 and the snap-tag coding sequence amplified from pSNAP-tag (T7)-2 (New England Biolabs, NEB; Supplementary Information) using the primer pair 9631/9632 ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... MBP-CENP-E was cloned into pMal-C2X (NEB). Bacterially-expressed constructs of GST-CENP-E were cloned in pET-3aTr (Tan ...
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bioRxiv - Microbiology 2019Quote: ... or Bam HI and Xho I (New England BioLabs, MA). The column isolated DNA fragments were ligated into our modified pFastBac1 plasmid having 6xHis tag at C-terminus ...
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bioRxiv - Molecular Biology 2020Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 (Illumina).
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bioRxiv - Microbiology 2021Quote: ... Followed by subcloning the PCR amplicon in Bam-HI (NEB) restriction digested pCW57-tGFP-2A-MCS plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... using NEBuilder Hi-Fi Assembly (New England Biolabs, Cat. #E2621). The pMB1052 construct was subsequently cut using BspDI and EcoRV (New England Biolabs) ...
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bioRxiv - Genomics 2022Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... The Hi-Scribe T7 High Yield RNA synthesis kit (NEB) was used to generate RNA transcripts at 37°C for 16 hours ...
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bioRxiv - Systems Biology 2024Quote: ... using the NEBuilder Hi-Fi Assembly kit (New England Biolabs). To ensure representation of all variants in the population ...
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bioRxiv - Cancer Biology 2023Quote: ... cut with hi-fidelity EcoRI and BamHI (New England Biolabs), and gel purified ...
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bioRxiv - Genomics 2021Quote: ... coli BL21 DE3 cells (C2527, New England Biolabs) using the DNEasy Blood and Tissue kit (69504 ...
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bioRxiv - Microbiology 2021Quote: ... coli strain MG1655 (ATCC 47076) and DH5α (NEB) were grown in LB or MMB (LB supplemented with 0.1 mM MnCl2 and 5 mM MgCl2 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli Stbl (New England Biolabs, Ipswich, MA, USA) for in vivo cloning as previously described27 ...
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bioRxiv - Biophysics 2022Quote: ... coli BL21 (DE3)-pLyse S (NEB, Catalogue # C3010I) cells ...
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bioRxiv - Biochemistry 2020Quote: ... Escherichia coli strain NiCo21(DE3) (New England Biolabs) was transformed with the pLysS plasmid (Novagen ...