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Citations for New England Biolabs :
1251 - 1300 of 6947 citations for hsa mir 940 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... All PCRs in this section were carried out using Q5 PCR Mix (NEB), 5 ng template and appropriate primers in 20 μl reactions ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the Monarch PCR and DNA Cleanup Kit (NEB) and sequenced using the Sanger method with M13F or M13R primers.
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bioRxiv - Microbiology 2020Quote: PCR amplification was performed using high-fidelity Q5 Polymerase PCR (New England Biolabs) with the following primers sets ...
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bioRxiv - Bioengineering 2021Quote: ... PCR products were purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs). RT ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR amplifications were column-purified using the Monarch PCR & DNA Cleanup Kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... purified PCR products were cloned using NEB PCR Cloning Kit (New England BioLabs). Clones were Sanger sequenced at the University of Minnesota Genomics Center.
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR product was purified using Monarch® PCR & DNA Cleanup kit (NEB) and eluted in 10 μL of Ultra Pure™ DNase/RNase free distilled water ...
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR product was purified using Monarch® PCR & DNA Cleanup kit (NEB) and eluted in 10 μL of Ultra Pure™ DNase/RNase free distilled water ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR amplifications were column-purified using the Monarch PCR & DNA Cleanup Kit (NEB), and assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Genetics 2023Quote: ... PCR products were purified using the Monarch PCR & DNA Cleanup Kit (NEB T1030). Plasmids were transformed into electrocompetent E ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR-amplified or digested products were purified (Monarch PCR & DNA Cleanup Kit, NEB). Then ...
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bioRxiv - Genomics 2023Quote: ... Secondary PCRs were purified with the Monarch PCR and DNA Cleanup Kit (NEB), ligated into the pCR2.1-TOPO TA cloning vector (Invitrogen) ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR products were purified using a Monarch PCR Cleanup Kit (New England Biolabs) and prepared for nanopore long read sequencing using the Ligation Sequencing Kit (Oxford Nanopore Technologies ...
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bioRxiv - Genetics 2023Quote: ... PCR-1 and PCR-2 amplicons were digested with DpnI (NEB Cat#R0176S) for 2 hours at 37°C to remove the YCp50-WT_PKR template ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified using the Monarch PCR & DNA Cleanup Kit (NEB) and sent for Sanger sequencing (GENEWIZ).
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bioRxiv - Microbiology 2024Quote: ... PCR products were cleaned using the Monarch PCR & DNA cleanup kit (NEB, T1030L). VSG sequences were determined by amplicon nanopore sequencing performed by Plasmidsaurus using Oxford Nanopore Technology with custom analysis and annotation or Sanger sequencing with the sequencing primer (5’-AGAGAATACTAAGCTAGTTGGC-3’ ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μL RT Adapter (RTA)(SQK-RNA002) and 2 μL T4 DNA Ligase (NEB) were mixed together and incubated under 25 °C for 10 min ...
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bioRxiv - Bioengineering 2020Quote: ... the RT reaction mixture was diluted 40x into a HotStart Taq (New England Biolabs) PCR reaction (1 mM MgCl2 ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed with Luna Universal qPCR Master Mix (New England Biolabs #M3003) per the manufactures instructions on a CFX Connect instrument (Bio-Rad #1855200) ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was generated from RNA (Omicron-BA.1; EPI_ISL_7062525) by LunaScript RT SuperMix (NEB). PCR reactions using Q5® High-Fidelity DNA Polymerase were performed with the primers and templates described in Supplementary Information Table 3 ...
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bioRxiv - Cancer Biology 2022Quote: ... First-strand cDNA synthesis was performed using LunaScript RT SuperMix Kit (NEB, cat. E3010S). qPCR was performed on a QuantStudio 3 or StepOnePlus real-time PCR machine using TaqMan Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was reverse transcribed into cDNA using a LunaScript RT SuperMix Kit (NEB, E3010L) by following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... 5’ RACE products were generated with Template Switching RT Enzyme Mix (New England Biolabs) using anchored poly(dT)23 and TSO (GCT AAT CAT TGC AAG CAG TGG TAT CAA CGC AGA GTA CAT rGrGrG ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by annealing at RT for 2h and subsequently ligation with T4 ligase (NEB) into the BsmBI-digested (Fermantas ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg RNA was reverse transcribed to cDNA using LunaScript RT SuperMix Kit (NEB). qPCR was performed in 20 μl reactions containing diluted cDNA ...
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bioRxiv - Microbiology 2023Quote: ... RNA immunoprecipitations and inputs were converted to cDNA using LunaScript RT SuperMix Kit (NEB). m6A-immunoprecipitated samples were normalised to their respective input samples and m6A content at a particular region calculated relative to an unmodified control region within the same transcript.
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bioRxiv - Genetics 2023Quote: ... Reactions were performed using the Luna Universal One-Step RT-qPCR Kit (NEB, E3005L) and a CFX Opus 384 Real-Time PCR System (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... The Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Cat # E3005X) and reaction protocol (cycle variations ...
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A laboratory framework for ongoing optimisation of amplification based genomic surveillance programsbioRxiv - Genomics 2023Quote: ... cDNA was generated for all samples using LunaScript RT SuperMix Kit (New England BioLabs). Sufficient volume was prepared to perform serial dilutions (10−2 to 10 -7 ...
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bioRxiv - Biochemistry 2023Quote: ... both the Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB M3019) and the Luna Universal Probe One-Step RT-qPCR Kit (NEB E3006 ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was converted to cDNA using LunaScript RT SuperMix Kit (NEB, cat. no. E3010) according to manufacturer specifications ...
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bioRxiv - Cancer Biology 2022Quote: ... All ligations were carried out at RT using T4 DNA ligase (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... RNA (200 ng) was reverse transcribed into cDNA using LunaScript®RT SuperMix (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... We performed cDNA synthesis from RNA using LunaScript RT Supermix Kit (New England Biolabs) beginning with 500ng of RNA ...
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bioRxiv - Genomics 2023Quote: ... 0.4 μL RNase Inhibitor and 2 μL Induro RT (200 U/μL; NEB M0681S) were incubated for 15 minutes at 60°C ...
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bioRxiv - Systems Biology 2023Quote: ... The reverse transcription reaction mix (1x RevertAid RT buffer, 250 μM dNTPs (NEB N0447L), 0.2 mg/mL recombinant albumin (NEB B9200S) ...
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bioRxiv - Genomics 2024Quote: ... Reverse transcription was performed with dT priming using the ProtoScript II RT (NEB M0368L) as described by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA (>300 ng) was reverse transcribed using the Lunascript RT Supermix Kit (NEB, E3010).
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bioRxiv - Cell Biology 2023Quote: The novel entry modules created for this study were cloned by insertion of PCR-amplified sequences in pJet1.3 or pMiniT 2.0 following manufacturer’s instructions (CloneJET PCR Cloning Kit, Thermo Scientific; NEB® PCR Cloning Kit, NEB). BsaI recognition sites followed by module-specific overhangs were added to each primer used to amplify entry sequences ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were column-purified (Monarch PCR & DNA Clean-up Kit, New England BioLabs) and submitted for Sanger sequencing (Genewiz ...
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bioRxiv - Immunology 2020Quote: ... Each PCR mix contained of 25µl of NEB 2x PCR Mix (New England Biolabs), 2.5µl of 25µM forward primer (Primer Ad1_noMX) ...
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bioRxiv - Bioengineering 2021Quote: ... Double stranded preamplifier synthesis template was synthesized by PCR using LongAmp PCR polymerase (NEB), non-phosphorylated forward primer ...
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bioRxiv - Immunology 2022Quote: ... and PCR amplified and barcoded with NEBNext High-Fidelity PCR Mix (New England Biolabs) and unique dual indexes (Illumina) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR indexing was performed using NEBNext High-Fidelity 2x PCR Master Mix (M0541S, NEB) and sequenced using a NextSeq platform (Illumina) ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were amplified by PCR using NEBNext High-Fidelity 2x PCR Master Mix (NEB) using PCR primers Ad1_noMX and Ad2_Barcode (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified (Monarch® PCR & DNA Cleanup Kit 5µg; New England BioLabs) and sequenced from both sides with Eurofins Genomics ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR was performed on plasmid DNA with Phusion High Fidelity PCR Kit (NEB). PCR products were converted to RNA using the T7 RNA Transcription Kit (NEB ...
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bioRxiv - Physiology 2020Quote: ... was amplified by PCR (Phusion High-Fidelity PCR Master Mix, NEB, Hitchin, Hertfordshire, UK) using specific oligonucleotide primers (5’-TCGCTACTGTTTCTCTCGCA-3’ and 5’-AAAGGCGTCAACAACTGCTT-3’) ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR product was purified using the Monarch PCR & DNA clean-up kit (NEB). All library preparation and sequencing was carried out at the Vanderbilt Technologies for Advanced Genomics (VANTAGE ...
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bioRxiv - Cell Biology 2022Quote: ... sgRNA inserts were PCR-amplified using NEBNext High Fidelity PCR Master Mix (NEB, M0541). The resulting PCR amplicons were purified using Ampure XP Beads and sequenced using the HiSeq 2500 system (Illumina ...