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Citations for New England Biolabs :
1201 - 1250 of 6947 citations for hsa mir 940 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... cDNA was prepared from viral RNA using random primer mix (NEB, Massachusetts, United States), and LunaScript® RT SuperMix Kit (Cat# E3010G) ...
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bioRxiv - Cell Biology 2022Quote: ... and Calnexin (membrane) using qPCR primers (IDT) with Q5 polymerase (New England Biolabs, M0492) was measured with a highly sensitive dsDNA detection dye (Lumiprobe ...
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bioRxiv - Developmental Biology 2022Quote: ... with SP6 and T3 primers and Phusion High Fidelity DNA polymerase (New England, BioLabs). The purified product was used as a template for the transcription reaction using the mMessage mMachine SP6 RNA transcription kit (AM1340 ...
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bioRxiv - Microbiology 2022Quote: ... These primer pairs were assembled by heat-denaturation and annealing in Buffer 3.1 (NEB), then cloned into BbsI-linearized pX458 (62 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The DNA template for mega primer generation was amplified using Q5 DNA polymerase (NEB) and the T7 and T7term primer from EOY4D2.1@pet21(+) ...
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bioRxiv - Biochemistry 2022Quote: ... using the Q5 site-directed mutagenesis kit and primer design tools (New England BioLabs) with the following primer pair:
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bioRxiv - Microbiology 2023Quote: ... 0.5 µM of each primer and 1.25 U Taq DNA polymerase (New England Biolabs). DNA amplification was performed in a MyCycler thermal cycler (BioRad ...
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bioRxiv - Genetics 2023Quote: ... 200 nM target primers and 10 U of Hind III–HF restriction enzyme (NEB) in a final volume of 20 μL ...
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bioRxiv - Cancer Biology 2023Quote: ... amplification of extracted gDNA by PCR using a barcoded forward primer (CC_fwd1 to CC_fwd20) and a barcoded reverse primer (CC_Cri_a_rev1 to CC_Cri_a_rev20) using Q5 polymerase (NEB M0491L). Pooled libraries were sequenced on an Illumina NextSeq 500 platform using a 75 bp single read on a high output flow cell with a 2–5% PhiX spike-in ...
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bioRxiv - Molecular Biology 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB #E6440S). Library cleanup was performed prior to and after PCR amplification using 0.8X Kapa Hyperpure beads (Roche #08963851001) ...
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bioRxiv - Cell Biology 2023Quote: ... using primers synthesized by IDT and a Q5® Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Neuroscience 2023Quote: ... 10 µM of each primer was combined with OneTaq QuickLoad Master Mix (NEB #M0486) and run in a thermocycler as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... the adaptor complementary primer was labeled with [γ-32P]-ATP and T4 PNK (NEB) using the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... The annealed primers were extended to fully double stranded DNA using Klenow polymerase (NEB). The barcode was purified by phenol-chloroform extraction ...
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bioRxiv - Molecular Biology 2023Quote: ... Excess primers were degraded by adding 0.5 μL of thermolabile exonuclease I (NEB m0568) at 37 °C for 15 minutes ...
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bioRxiv - Genomics 2023Quote: ... Excessive primers were digested with the addition of 0.5 μL Exonuclease I (NEB M0293S), and incubation at 37°C for 30 min and 72°C for 20 min ...
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bioRxiv - Biochemistry 2023Quote: ... reactions were performed with mutagenesis primers and the Q5 DNA polymerase (New England Biolabs). Afterward ...
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bioRxiv - Neuroscience 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB E6440S). Library cleanup was performed prior to and after PCR amplification using 0.8X Kapa Hyperpure beads (Roche 08963851001) ...
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bioRxiv - Biochemistry 2023Quote: ... Primers for generating mutations for MsbA were designed using the online tool NEBaseChanger (NEB) and carried out using the KLD enzyme mix (NEB ...
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bioRxiv - Plant Biology 2019Quote: Library preparation was performed with the NEBNext® Multiplex Small RNA Library Prep Set for Illumina (New England BioLabs) according to the manufacturer’s instructions with few deviations ...
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bioRxiv - Evolutionary Biology 2020Quote: ... library preparation was carried out using NEBNext Multiplex Small RNA Library Prep Set for Illumina kit (New England Biolabs) with modified protocol23 for improving low-input samples and sequenced with NextSeq500 (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... Small RNA libraries were constructed using NEBNext® Small RNA Library Prep Set for Illumina® (Cat. # E7330L, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Small RNA libraries were prepared according to the manufacturer’s protocol of the NEBNext Multiplex Small RNA Sample Prep Set for Illumina-Library Preparation (New England Biolabs). NEBNext Multiplex Oligos for Illumina Index Primers were used for library preparation (New England Biolabs) ...
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bioRxiv - Developmental Biology 2019Quote: ... This was used as input for the NEBNext Multiplex Small RNA Library Prep Set for Illumina kit (NEB #E7300) according to the manufacturer’s recommendations with the following modifications ...
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bioRxiv - Biochemistry 2021Quote: ChIP-seq library construction was performed using NEBNext ChIP-Seq Library Prep Master Mix Set (E6240, NEB, Ipswich, MA) following the manufacturer’s instruction with modifications ...
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bioRxiv - Plant Biology 2020Quote: Flax small RNA libraries were constructed using the NEBNext Small RNA Library Preparation set for Illumina (NEB, cat # E7300); for columbine and rose ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA libraries were constructed using the NEBNext mRNA Library PrepReagent Set for Illumina (New England Biolabs, Ipswich, MA USA) following the manufacturer’s protocol and were subjected to Illumina sequencing ...
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bioRxiv - Neuroscience 2021Quote: Twelve small RNA libraries representing 2 biological and 3 technical replicates from control- or BDNF-treated cortical neurons and corresponding EVs were prepared using NEBNext_Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) as per manufacturer’s instructions and sequenced in Illumina HiSeq2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... deep sequencing libraries were generated using the NEBNext Small RNA Library Prep Set for Illumina (E7560, New England Biolabs) and sequenced on an Illumina Hiseq4000 ...
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bioRxiv - Cell Biology 2022Quote: ... Complementary DNA libraries were then prepared using a range of mitochondrial RNA inputs (final input range 1.8-120 ng) using the NEBNext Multiplex Small RNA Library Prep Set (New England Biolabs.). To investigate the efficiency of small RNA library preparation across a range of mitochondrial RNA inputs ...
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bioRxiv - Genomics 2022Quote: ... ChIP-seq libraries were prepared using NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (New England BioLabs). Rabbit polyclonal antibody for Rad21 has been described in39 ...
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bioRxiv - Cancer Biology 2022Quote: The same sets of RNA samples were reverse transcribed using Protoscript II First Strand cDNA Synthesis Kit (NEB #E6560S). cDNA was measured using a QuantStudio 7 Flex Real-time PCR machine (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... The sequencing library was generated by NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA), with added index codes to attribute sequences of each sample as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2020Quote: ... Sequencing libraries were generated using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Illumina libraries were then prepared using the NEBNext DNA Sample Prep Master Mix Set (New England Biolabs, MA, USA) and DNA fragments were PCR-amplified using Platinum Pfx DNA polymerase (Invitrogen ...
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bioRxiv - Genetics 2020Quote: ... PCR analysis was carried out with different primer sets (Supplementary Table 1, Fig. 2B) using Phusion High-Fidelity DNA Polymerase following the manufacturer’s instructions (New England Biolabs).
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bioRxiv - Genomics 2021Quote: ... DNA libraries were constructed using the NEBNext DNA Library Prep Master Mix Set for Illumina (NEB E6040, M0543L, E7335S). To elute DNA ...
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bioRxiv - Cell Biology 2022Quote: ... Small RNA libraries were prepared according to the manufacturer’s protocol of the NEBNext Multiplex Small RNA Sample Prep Set for Illumina-Library Preparation (New England Biolabs). The required number of PCR cycles to amplify library is determined by running PCR for 10-15 cycles and analyzing PCR products on a 6% PAGE gel ...
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bioRxiv - Immunology 2022Quote: ... Sequencing libraries were prepared using NEBNext® Fast DNA Library Prep Set for Ion Torrent™ (New England BioLabs), diluted to the same concentration and pooled ...
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bioRxiv - Neuroscience 2022Quote: ... and libraries were prepared using the NEBNext Fast DNA Library Prep Set for Ion Torrent (New England BioLabs, UK) and the Ion Xpress Barcode Adapters 1-96 Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... We pooled the second set of eBlocks at equal molar ratio and used Esp3I Type IIs restriction enzyme (NEB), NEBridge Ligase Master Mix ...
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bioRxiv - Genetics 2023Quote: ... Small RNA libraries were prepared according to the manufacturer’s protocol of the NEBNext Multiplex Small RNA Sample Prep Set for Illumina-Library Preparation (New England Biolabs). NEBNext Multiplex Oligos for Illumina Index Primers were used for library preparation (New England Biolabs) ...
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bioRxiv - Genetics 2023Quote: Libraries were prepared according to the manufacturer’s protocol of the NEBNext Multiplex Small RNA Sample Prep Set for Illumina-Library Preparation (New England Biolabs). NEBNext Multiplex Oligos for Illumina Index Primers were used for library preparation (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... Reactions were set up as per manufacturer’s instructions with the addition of 4 units of Murine RNase Inhibitor (NEB) and 5% DMSO ...
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bioRxiv - Plant Biology 2023Quote: ... This was followed by two sets of enzymatic conversion steps using the Enzymatic Methyl-seq kit (NEB, Cat# E7120L) to differentiate cytosines from 5mC and 5hmC ...
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bioRxiv - Molecular Biology 2023Quote: ... sequencing libraries were generated using a NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2024Quote: ... The PCR reaction was set up with 1 µL of the supernatant in a 12.5 µL reaction with Taq polymerase (NEB), according to manufacturer’s instruction.
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bioRxiv - Microbiology 2019Quote: ... PCR was done with the Phusion High-Fidelity PCR kit (New England BioLabs) with 40ng of gDNA ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were cleaned up using the Monarch PCR Cleanup kit (T1030L, NEB). A double digest using PacI and AscI was done on the PCR products and the pcs2+MCS-P2A-sfGFP plasmid ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA was PCR amplified with Phusion High-fidelity PCR mastermix (NEB M0530) and Truseq PCR primers ...