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Citations for New England Biolabs :
1101 - 1150 of 6947 citations for hsa mir 940 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Next the 3’ primer regions were truncated using the BciVI restriction endonuclease (NEB), followed by 2.0x AMPure XP cleanup ...
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bioRxiv - Genetics 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique index primer pairs) (E6440, NEB). We used 13 min fragmentation time and 9 cycles of PCR enrichment on 1000 ng of total RNA input determined by preliminary library preparation ...
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bioRxiv - Physiology 2023Quote: ... using the first strand cDNA synthesis standard protocol with random primers (S1330, NEB), 2.5 mM dNTPs (N0446 ...
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bioRxiv - Bioengineering 2023Quote: ... The mutated samples were addressed to amplification using primers and Q5U from NEB following the PCR setting of annealing temperature of 63 °C and an extension of 72 °C at 45 seconds ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µM of primers were combined with OneTaq QuickLoad Master Mix (NEB #M0486) and run in a thermocycler as follows ...
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bioRxiv - Synthetic Biology 2023Quote: ... The same primers were used to guide RCA with Phi29 polymerase (NEB M0269). Following PCR or RCA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and 0.3 μM Dual Index primers of NEBNext Multiplex Oligos for Illumina (NEB). Three independent PCR reactions from the same library were combined after PCR and purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Immunology 2023Quote: ... and excess primers were removed with an Exonuclease I digestion (New England Biolabs). Whole transcriptome amplification (WTA ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR were performed using Q5 PCR master mix and One-Taq quick load master mix for colony PCR (NEB), primers were purchased from IDT (Louvain ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR were performed using Q5 PCR master mix and One-Taq quick load master mix for colony PCR (NEB), primers were purchased from IDT (Louvain ...
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bioRxiv - Plant Biology 2019Quote: ... All sRNA libraries were made using the NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs) following the manufacturer instructions and using gel-enriched sRNAs as described in Martinez et al (2016).
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bioRxiv - Molecular Biology 2019Quote: ... or prepared for sequencing using the NEBNext DNA Library Prep Master Mix Set for Illumina (New England BioLabs). ChIP-seq data are available on the NCBI GEO database (GSE128317).
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bioRxiv - Microbiology 2019Quote: ... genomic DNA libraries were prepared using NEBnext DNA Library Prep Master Mix Set for Illumina (New England Biolabs) following the instructions for 400 bp insert libraries ...
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bioRxiv - Microbiology 2021Quote: ... Small RNA libraries were prepared using NEB Next® Small RNA Library Prep Set for Illumina (NEB, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Small RNA libraries were constructed using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs). mRNA and small RNA libraries were sequenced on Illumina HiSeq2500 instrument ...
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bioRxiv - Molecular Biology 2022Quote: miRNA libraries were constructed using Multiplex Small RNA Library Prep Set for Illumina (E7508L, New England Biolabs, Netherlands). ssDNA was captured in Illumina flow cell and used for in situ amplification ...
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bioRxiv - Molecular Biology 2020Quote: Libraries were made from the above protected fragments using the NEBNext ChIP-Seq MasterMix Set (New England Biolabs) with the following modifications ...
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bioRxiv - Evolutionary Biology 2020Quote: Sequencing libraries were built using NEBNext DNA Library Prep Master Mix Set for 454 (E6070, New England Biolabs) and Illumina-specific adaptors66 following established protocols66–68 ...
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bioRxiv - Genetics 2019Quote: ... and used for library construction using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: The sRNA-seq libraries were constructed with NEB Next Multiplex Small RNA Library Prep Set for Illumina (NEB). After agarose gel electrophoresis ...
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bioRxiv - Molecular Biology 2022Quote: Small RNA libraries were prepared with the NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs) following the protocol provided by the manufacturer ...
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bioRxiv - Bioengineering 2023Quote: All qPCR reactions were set up using 7.5 µl of 2 x Luna Universal qPCR master mix (NEB), 0.375 pmol forward primer ...
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bioRxiv - Cell Biology 2023Quote: ChIP libraries were prepared using NEBNext ChIP-seq DNA Sample Prep Master Mix Set for Illumina (NEB E6240) and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Library preparation was performed on beads using NEBNext ChIP-Sequencing Library Prep Master Mix Set for Illumina (NEB). The manufacturer’s instructions were followed ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were prepared using the NEBNext® Small RNA Library Prep Set for Illumina® (New England BioLabs), with the Universal miRNA Cloning Linker (New England BioLabs ...
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bioRxiv - Genomics 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1) (New England BioLabs E7416). DNA libraries were quality checked with DNA 1000 assay (Aligent 5067-1504 ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by end-repair and dA-tailing using ChIP-Seq Library Prep Reagent Set for Illumina (NEB, E6200L). DNA was incubated for 3 min at 95℃ and returned to room temperature for kinetic enrichment for single-stranded DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... Small RNA libraries were constructed using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted DNA fragments were then amplified by PCR with Nextera compatible indexed sequencing i5 and i7 adapters using NEBNext 2x PCR Master Mix PCR kit (M0541, NEB). The amplified DNA library was fragment size selected from 200bp to 800bp using Ampure XP beads (A63880 ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was PCR amplified (primers hFUR Exon 1-3 PCR For and hFUR Exon 1-3 PCR Rev) and purified (Monarch PCR & DNA cleanup kit, NEB). The resulting PCR product was sequenced using the hFur Exon 1 Seq primer.
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bioRxiv - Evolutionary Biology 2023Quote: ... The eluted DNA fragments were then amplified by PCR with Nextera compatible indexed sequencing i5 and i7 adapters using NEBNext 2x PCR Master Mix PCR kit (M0541, NEB). The amplified DNA library was fragment size selected from 200bp to 800bp using Ampure XP beads (A63880 ...
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bioRxiv - Immunology 2023Quote: PCR was performed in a 40 μl PCR reaction containing 1x Q5 HotStart Hifi PCR master mix (New England Biolabs), 0.4 μM of Illumina adapter PCR primers (ILM_p5_PCR ...
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bioRxiv - Immunology 2022Quote: ... Sample index PCR: 2 µl tagging PCR product was mixed with 18 µl PCR mix (4 µl 5x phusion HF buffer (NEB); 0.4 µl Phusion DNA Polymerase (NEB) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... twelve-cycle PCR reactions (Phusion High Fidelity PCR kit, New England Biolabs), using primers that added Flow Cell binding sequences ...
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bioRxiv - Biochemistry 2022Quote: ... Following which the PCR products were purified by PCR cleanup kit (NEB) and a second PCR was done with primers containing the READ1 ...
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bioRxiv - Immunology 2022Quote: ... a quantitative PCR reaction (10 µl 2 x PCR master mix (NEB), 1 x SYBR green (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR reactions were purified with Monarch PCR & DNA Cleanup Kit (NEB, T1030L). For the hHBB-Fluc control mRNA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were generated using the 2x Q5 PCR Master Mix (NEB) in accordance with manufacturer instructions ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... Resulting PCR product was purified using Monarch PCR purification kit (NEB, USA) and were cloned into pCS2+ vector using restriction enzymes BamH1 and SnaB1 based cloning to generate pCS2-Satb2 ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR product was purified using Monarch PCR & Cleanup Kit (NEB T1030S) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR products were cleaned (Monarch PCR & DNA Cleanup Kit, NEB cat. T1030S) and Sanger-sequenced ...
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bioRxiv - Microbiology 2022Quote: ... Products were PCR cleaned up with Monarch PCR & DNA cleanup kit (NEB), ligated into pCR2.1-TOPO vector (Thermo fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were subsequently PCR amplified using Phusion HF PCR Master Mix (NEB) with the following PCR program ...
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bioRxiv - Genetics 2023Quote: ... and PCR products were then purified by PCR & DNA Cleanup Kit (NEB). STR PCR products were ligated with the pClone007 simple vector (Tsingke) ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were purified using the Monarch PCR Purification Kit (NEB # T1030L) and eluted in 10µl elution buffer.
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Genomic dissection and mutation-specific target discovery for breast cancer PIK3CA hotspot mutationsbioRxiv - Genomics 2024Quote: ... PCR was performed using Phusion High-Fidelity PCR Master Mix (NEB, M0531) and the enhancer deletion validation primer set (Table S5) ...
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bioRxiv - Microbiology 2020Quote: ... and reverse-transcripted to cDNA using LunaScript RT SuperMix kit (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: cDNA synthesis reactions were carried out using the Lunascript RT Supermix kit (NEB) and 500 ng of RNA was used for each reaction.
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bioRxiv - Immunology 2021Quote: ... Luna Universal Probe One-step RT-qPCR kit (New England Biolabs, Ipswich, MA) was used to amplify the envelope (E ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was prepared using LunaScript™ RT SuperMix Kit (New England Biolabs, E3010S). Real-time PCR was performed using Luna® Universal qPCR Master Mix (New England Biolabs ...