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Citations for New England Biolabs :
1051 - 1100 of 7433 citations for hsa mir 940 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... using the Luna Universal One-Step RT-qPCR Kit (NEB, #E3005). No template and genomic DNA controls were included in all experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... Luna® Universal One-Step RT-qPCR Kit (NEB, Ipswich, Massachusetts) was used to quantify the RNA samples per the manufacturer’s guidelines ...
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Sphingosine induction of the Pseudomonas aeruginosa hemolytic phospholipase C/sphingomyelinase, PlcHbioRxiv - Microbiology 2023Quote: ... cDNA was generated using the Induro RT reverse transcriptase system (NEB). PCRs were conducted using Q5 DNA polymerase (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The Luna Universal One-Step RT-qPCR Kit (New England Biolabs) was used for the detection of viral genomes in the heat-inactivated samples performed through reverse transcription quantitative polymerase chain reaction (RT-qPCR) ...
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bioRxiv - Genomics 2023Quote: Each library was converted to cDNA with Induro RT (NEB M0681S) using a 57-mer ssDNA as a primer ...
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was performed with Luna Universal qPCR Master Mix (Biolabs) using the Step-One Real-time PCR system (Life Technologies) ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA synthesis was performed using LunaScript® RT SuperMix Kit (NEB) following provider recommendation ...
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bioRxiv - Genetics 2021Quote: ... The forward primer was phosphorylated with T4 polynucleotide kinase (New England Biolabs) and used in a high fidelity PCR reaction (see below ...
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bioRxiv - Developmental Biology 2020Quote: ... by using primers with BamHI-HF (New England Biolabs, 20U/ml, #R3136S) and XbaI (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... single primer site-directed mutagenesis was performed with Q5 DNA polymerase (NEB) and primer #8964 to change the Cys21 codon (TGT ...
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bioRxiv - Genomics 2019Quote: ... Universal and index primers from NEBNext Multiplex Oligos for Illumina (NEB #E7335S) were incorporated by a 12 cycle PCR ...
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bioRxiv - Molecular Biology 2019Quote: ... and 1 μL of Index (X) Primer (25 mmol) (NEB, Ipswich, USA). The reaction conditions were as follows ...
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bioRxiv - Systems Biology 2019Quote: ... DNA samples were amplified using Illumina indexing primers (NEB Catalog no. 7600L). PCR reactions were performed in 16 separate 20 μl aliquots on beads involving 8 cycles of amplification (98°C for 90 seconds followed by 98°C for 15s ...
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bioRxiv - Systems Biology 2019Quote: ... 72°C for 30s) using Illumina indexing primers (NEB Catalog No. 7500L). DNA was purified using Ampure XP beads and sequenced on Illumina HiSeq 2500 to generate 75 bp paired-end reads ...
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bioRxiv - Biophysics 2021Quote: ... Primers were designed using the NEBuilder tool (New England Biolabs, Ipswitch, MA), inserts were synthesized as gBlocks and purchased from Integrated DNA Technologies (Integrated DNA Technologies ...
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bioRxiv - Systems Biology 2021Quote: ... with either single index or dual index primers also obtained from NEB. We followed the manufacturer’s instructions except for the following variations:
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bioRxiv - Microbiology 2021Quote: ... The primers were obtained using the NEBuilder Assembly Tool (New England Biolabs). After digestion by DpnI (New England Biolabs ...
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bioRxiv - Synthetic Biology 2021Quote: ... the forward primers were phosphorylated using T4 polynucleotide kinase (New England Biolabs) to facilitate T4 ligation later ...
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bioRxiv - Cell Biology 2021Quote: ... from pSJ1256 using primers containing the PacI and AscI (New England BioLabs) restriction sites ...
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bioRxiv - Microbiology 2022Quote: ... excessive primers were removed with an enzymatic protocol using exonuclease I (NEB) and quick CIP (NEB).
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bioRxiv - Developmental Biology 2022Quote: ... we reverse-transcribed cDNA with a random primer mix (NEB, MA, USA) and GoScript™ Reverse Transcriptase (Promega ...
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bioRxiv - Developmental Biology 2019Quote: ... Ligated fragments were amplified using dual-indexed primers for Illumina (NEB #E7600S) for 8-10 PCR cycles ...
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bioRxiv - Genetics 2020Quote: ... used dual index primers (from NEBNext Multiplex Oligos for Illumina, NEB E7600S) for multiplexing ...
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bioRxiv - Pathology 2021Quote: ... using custom synthesized complementary primers (IDT) and the Gibson assembly kit (NEB). After verifying the DNA sequence of the pcDNA3-4 fused with the cDNA for ace2 1-618-ddc-abd ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was generated using oligo (dT) 18 primer (New England Biolabs, 513165) and SuperScript II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: The NNS primers were phosphorylated with T4 polynucleotide kinase (NEB, cat#M0201S). 20 μL phosphorylations was prepared according to the following recipe ...
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bioRxiv - Genomics 2021Quote: ... excess primers were removed using an Exonuclease I digestion (New England Biolabs), second strand synthesis was performed ...
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bioRxiv - Genomics 2021Quote: Purified RNA (5 ug) was reverse transcribed using Random Primer 9 (NEB) and SuperScript II reverse transcriptase under error prone conditions as described Smola et al. ...
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bioRxiv - Genomics 2021Quote: Purified RNA (9 μg) was reverse transcribed using Random Primer 9 (NEB) and SuperScript II reverse transcriptase under error prone conditions as described in Smola et al. ...
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bioRxiv - Immunology 2020Quote: ... RNA and primer dimers were digested using RNAseA and Lambda Exonuclease (NEB) after which cDNA was amplified using the Kapa Biosystems HiFi HotStart ReadyMix (2X ...
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bioRxiv - Immunology 2020Quote: ... Beads were then treated with ExoI to digest unoccupied primer sites (NEB).
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bioRxiv - Genomics 2022Quote: ... specific primers (Supplementary Table S1) were radiolabelled with T4 Polynucleotide kinase (NEB) and [?-32P]ATP (6,000 Ci/mmol) ...
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bioRxiv - Plant Biology 2022Quote: ... and ZmCYP71Z16 using gene-specific primers and Phusion polymerase (New England BioLabs) according to manufacturer instructions ...
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bioRxiv - Genomics 2022Quote: ... 20μM random primers (Thermo, SO181) and 1mM dNTP (NEB, N0446S and N0459S), then they were incubated at 98°C for 3min and immediately cooled down at 4°C for 20min ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized using a random primer mix (New England Biolabs, S1330S) and ProtoScript® II reverse transcriptase (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... was mutated using Q5 site directed mutagenesis kit (NEB, primers: SP (RSV) AP2 (hTFAP2A)-CRISPR RES-fw and SP (RSV ...
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bioRxiv - Developmental Biology 2024Quote: ... A primer extension reaction was performed using Phusion DNA polymerase (M0530L, NEB) followed by QIAquick purification (28106 ...
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bioRxiv - Biochemistry 2024Quote: ... followed by cDNA synthesis with oligoT primers (ProtoScript II, New England Biolabs). The TaPOR2 gene lacking the part coding a transit peptide and pET15b plasmid (Novagen ...
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bioRxiv - Molecular Biology 2023Quote: ... dinucleotide primers were 5’-labeled using T4 polynucleotide kinase (New England Biolabs) and added at 20 µM without pre-annealing to the template ...
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bioRxiv - Bioengineering 2024Quote: ... Primers for SDM (see Supplementary Table S3) were designed using NEBaseChanger (NEB) and synthesized as standard DNA oligos (IDT) ...
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bioRxiv - Cell Biology 2024Quote: ... using primers flanked by ClaI and XbaI restriction enzymes (New England Biolabs), digested and inserted into pcDNA3-TTP-mycHis vector backbone digested with the same restriction enzymes ...
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bioRxiv - Molecular Biology 2021Quote: PCR was performed with Taq polymerase PCR (New England Biolabs) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... PCR clean-up (Monarch PCR and DNA Cleanup Kit, NEB) and gel extraction (Monarch Gel Extraction Kit ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were purified (Monarch PCR Cleanup Kit (NEB, #T1030S)) and restriction digested with unique 5’ SpeI and 3’ PspXI sites ...
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bioRxiv - Microbiology 2020Quote: ... PCR was done using LongAmp® Taq PCR Kit (NEB) from 40 ng of QMA0248 S ...
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bioRxiv - Genetics 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Developmental Biology 2023Quote: ... before PCR using NEB 2xphusion PCR mastermix (NEB, cat #M0531S).
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bioRxiv - Synthetic Biology 2024Quote: ... PCR were performed using 2X Q5 PCR master mix (NEB) and GoTaq master mix for colony PCR and genotyping (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by a library preparation with the NEBNext Fast DNA Library Prep Set for Ion Torrent (NEB). cDNA libraries were sequenced on an Ion Torrent Proton.