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Citations for New England Biolabs :
1151 - 1200 of 2232 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The three fragments were cloned into the pMBsacB plasmid using 2x Hifi Master Mix (M5520AA; NEB) according to manufacturer’s instructions creating the plasmid pLT001.
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bioRxiv - Synthetic Biology 2022Quote: ... The target gene sequences were PCR amplified from the purchased plasmids using either Phusion (NEB, M0531S) or Q5 High Fidelity (NEB ...
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bioRxiv - Plant Biology 2022Quote: ... Plasmids were also confirmed through digestion with the restriction enzyme EcoRI (New England Biolabs; Ref.: R3101S) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Transfection plasmids were constructed by Gibson assembly using NEB Gibson Assembly Master Mix (New England Biolabs). Supplementary Table 1 provides the sequences of all primers used in this study.
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bioRxiv - Systems Biology 2023Quote: ... we pelleted each liquid culture by centrifugation and used the Monarch Plasmid Miniprep Kit (NEB #T1010L) to isolate plasmid according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Plasmids for the trans-Tango components were generated using the Hi-Fi DNA Assembly (NEB #E5520S), BP Gateway Cloning (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid pAR128 was generated by digesting pPL5920 with MluI-HF and NsiI-HF (New England Biolabs), PCR amplifying the UME6 locus (chrIV ...
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bioRxiv - Molecular Biology 2023Quote: ... CX plasmid (Bukhari et al., 2016) and 5′UTR containing amplicon was digested with NheI (NEB) followed by ligation with T4DNA ligase (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... pDONR/Zeo plasmid was linearized via Polymerase Chain Reaction (PCR) with the Q5 DNA polymerase (NEB) and a pair of primers for each Cac1 mutant that anneal to the sequences flanking the section to be modified (Supplementary Table 2) ...
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bioRxiv - Developmental Biology 2023Quote: ... the pUC18 plasmid was subjected to digestion with the EcoRI-HF restriction enzyme (New England Biolabs), followed by a standard ligation reaction ...
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bioRxiv - Bioengineering 2023Quote: ... Gene inserts were first assembled into the part plasmid pYTK001 using Esp3I (NEB, Ipswich, MA, USA) unless mentioned otherwise (Lee et al ...
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bioRxiv - Microbiology 2023Quote: ... to remove residual plasmid DNA and then purified using Monarch RNA cleanup kit (New England Biolabs). RNA was reverse transcribed using SuperScript IV (Invitrogen Life Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... The purified PCR product and pET28a plasmid were incubated with NheI and BamHI restriction enzymes (NEB) following the manufacturer’s protocol for 3 hours ...
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bioRxiv - Cell Biology 2023Quote: ... The Xlone-GFP backbone plasmid was linearized by treating with restriction enzymes SpeI-HF (NEB R3133L) and KpnI-HF (NEB R3142L) ...
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bioRxiv - Genetics 2023Quote: ... the 10XUAS-DSCP promoter was amplified from plasmid pNP with Q5 High-Fidelity DNA Polymerase (NEB) using primers UP0394 and UP0395 ...
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bioRxiv - Neuroscience 2023Quote: ... Antisense RNAs were synthesized from linearized plasmids using T3 RNA polymerase (New England Biolabs, Ipswich, MA), [α-35S]-CTP ...
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bioRxiv - Synthetic Biology 2023Quote: All PCR reactions used for plasmid assembly were done using the 2x Phusion mix from NEB, in a 50 µL volume ...
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bioRxiv - Developmental Biology 2023Quote: ... The amplicon was subcloned into pN2-CMV:RFP plasmid using XhoI/EcoRI restriction enzyme digestion-ligation (NEB) to generate pN2-CMV:AmRps14-RFP plasmids and sequence verified ...
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bioRxiv - Immunology 2023Quote: ... All fragments were assembled into a pLIX plasmid linearized by NheI and SalI (New England Biolabs) using HiFi DNA Assembly Kit (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... digested plasmid and amplicon were assembled using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: DNA templates for the transformation assays were prepared by PCR amplification from the pUC19 plasmid (NEB), after which the PCR fragments were Dpn1-treated ...
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bioRxiv - Biochemistry 2023Quote: PCR mutagenesis was performed by whole plasmid PCR with Q5 high-fidelity polymerase (New England Biolabs) followed by PCR purification (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: All plasmids were generated by using some combination of PCR amplification using Q5 DNA polymerase (NEB), HiFi Assembly (NEB ...
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bioRxiv - Cell Biology 2023Quote: All plasmids were assembled via Gibson assembly using the HiFi DNA Assembly mix (New England Biolabs), unless stated otherwise ...
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bioRxiv - Cell Biology 2023Quote: ... The blasticidin resistance gene was isolated from a plasmid pKM808 using XhoI/SalI (New England Biolabs) and treated with a Quick Blunting Kit (New England Biolabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli were used for cloning plasmids smaller than 10 kb and NEBStable (NEB cat no. C3040I) competent E ...
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bioRxiv - Synthetic Biology 2023Quote: ... Assembled plasmids were transformed in the commercial Escherichia coli DH5α competent cells (New England Biolabs (NEB), USA) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Assembled plasmids were transformed in the commercial Escherichia coli DH5α competent cells (New England Biolabs (NEB), USA) ...
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bioRxiv - Molecular Biology 2023Quote: ... the plasmid containing the origin DNA sequence was digested with HindIII and EcoRI (New England Biolabs) according to manufacturer’s protocol (Supplemental Figure S2A ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid constructs were created by Gibson cloning with NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621L) or Gibson Assembly Master Mix (NEB E2611L) ...
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bioRxiv - Bioengineering 2023Quote: ... plasmid pJC1-trpE (7749 bp) was amplified by PCR using oligonucleotides with degenerated (NNK) codons (NEB Q5 Site-Directed-Mutagenesis Kit ...
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bioRxiv - Biochemistry 2023Quote: Plasmids containing the α and β genes were freshly transformed in E.Coli BL21-DE3 cells (NEB). Transformed cells were plated on LB agar plates containing 100ug/ml Carbenicillin and grown overnight at 370C overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... small insertions or mutations were inserted into plasmids using Q5 site-directed mutagenesis (NEB CN# E0554S) according to manufacturer’s protocols and transforming chemically competent NEB 10-beta cells (NEB CN# C3019) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Template plasmid was then removed by addition of 1 μL DpnI (New England BioLabs, Cat.#R0176), 5 μL 10X FastDigest Buffer (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... FlucWT-HA-GFP11 and FlucDM-HA-GFP11 plasmids were constructed using site-directed mutagenesis kit (NEB) based on FlucSM-HA-GFP11 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the pAY24 plasmid digested with PstI and SmaI (New England BioLabs, #R0140 and #R0141, respectively). The sequence of all constructs was confirmed by Sanger sequencing at the Genomic Core Facility.
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bioRxiv - Cell Biology 2023Quote: ... mCh-Rab7A was cloned into a piggyBac plasmid using the NEB HIFI DNA Assembly Kit (NEB) and primers listed in Supp ...
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bioRxiv - Bioengineering 2023Quote: ... All plasmid construction was done by Gibson Assembly (HiFi DNA Assembly Master Mix, New England Biolabs) and validated by colony PCR and sequencing ...
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bioRxiv - Genomics 2024Quote: ... 4 μg of the barcoded plasmid library was linearized by digestion with NruI-HF (NEB #R3192) at 37°C for 16 hours ...
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bioRxiv - Microbiology 2024Quote: ... Chimeras were cloned in plasmid pSHU1234 using restriction digestion with NdeI and PstI (New England Biolabs). Plasmids carrying chimeric constructs were used as templates to generate the double-stranded donor DNA used to replace the motA and motB gene on the RP437 chromosome with pomA and potD variants ...
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bioRxiv - Genomics 2024Quote: ... Plasmid libraries were amplified using 1X NEBNext Ultra II Q5 Master Mix (New England Biolabs M0544), 1X EvaGreen dye (Biotium 31000) ...
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bioRxiv - Immunology 2023Quote: Plasmids with CAR constructs were linearized by digestion with the restriction enzyme SalI (New England Biolabs). mRNA was produced using the mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit ( AMB13455 ...
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bioRxiv - Genomics 2023Quote: ... Twist BioScience) and then assembled into pNL3.3 enhancer reporter plasmids using Gibson Assembly (New England Biolabs). Each cloned reporter plasmid was verified by Sanger sequencing (Quintara Bio) ...
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bioRxiv - Genomics 2023Quote: ... then ligated into a PB-CRISPRia expression plasmid linearized with BstXI and BlpI (New England Biolabs). Chemically competent Stable E ...
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bioRxiv - Synthetic Biology 2024Quote: The plasmid library containing static barcodes was generated by transforming high-efficiency competent cells (NEB C3019), then plating them onto a large surface area of LB-agar (∼30 10-cm petri dishes ...
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bioRxiv - Synthetic Biology 2024Quote: ... Purified plasmids were first amplified and linearized using Q5 Hot Start High-Fidelity DNA Polymerase (NEB); forward and reverse primers are listed in Table S5 ...
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bioRxiv - Genomics 2023Quote: ... then cloned into the EagI and EcoRI sites of the plasmid pJK03 with T4 ligase (NEB). The libraries were transformed into 5-alpha electrocompetent cells (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... We assembled the plasmids through Gibson Assembly using the NEBuilder HiFi DNA Assembly Master Mix (NEB) and followed the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: 10µg of a plasmid containing 807bp of Pcdh10 exon 1 sequence was linearized with SacII (NEB) in 1x CutSmart buffer (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... the linear plasmid obtained by PCR was ligated using the KLD enzyme mix (NEB cat # M0554S) according to the manufacturer’s protocol before transformation ...