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Citations for New England Biolabs :
1101 - 1150 of 2232 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... The amplicons and the linearized plasmid were assembled using an NEBuilder HiFi DNA assembly kit (NEB). The assembled plasmid was named pLenti-NG-PE2-BSD.
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bioRxiv - Biochemistry 2020Quote: ... The 186 bp fragment was excised from the plasmid by restriction digest with EcoRV (NEB: R0195) and EcoRI (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... the amplified DNA and the P4H-TM isoform 1 plasmid were both digested with BlpI (NEB) and XbaI (NEB ...
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bioRxiv - Neuroscience 2021Quote: ... Human α-synuclein cDNA from a pcDNA3.1 plasmid was restriction digested with EcoRI and HindIII (NEB), purified and ligated in the same sites of the pAAV2.5-THP backbone to generate the pAAV2.5-TH-α-syn plasmid ...
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bioRxiv - Synthetic Biology 2021Quote: ... Backbone plasmids were purified from gel after digestion using the Monarch DNA Gel Extraction Kit (NEB). Ligations were carried out in 96-well PCR plates by mixing 0.2 μL of digested plasmid (3-9 ng μL−1 ...
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bioRxiv - Genetics 2021Quote: All plasmids were constructed using Gibson-cloning with the NEBuilder HiFi DNA Assembly master mix (NEB). For the construction of the N-terminal part of the split NG-ABEmax-encoding vector (AAV-NT-ABEmax) ...
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bioRxiv - Microbiology 2021Quote: ... the plasmid-Safe DNase-treated DNA was amplified using phi29 DNA polymerase (New England Biolabs, USA) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... The pST76-A plasmid was cut with fast digest BsaI (New England Biolabs, Ipswitch, MA, USA) followed by phosphatase treatment (Fast AP ...
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bioRxiv - Biophysics 2021Quote: ... the agarose-gel purified assembled insert and plasmid backbone were digested with BamHI and XhoI (NEB) and the insert ligated with T4 ligase ...
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bioRxiv - Biophysics 2021Quote: Purified plasmids were digested at the 3’ end of the insert sequence with EcoRI-HF (NEB) to linearize the template with a 5’ overhang for in vitro transcription ...
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bioRxiv - Cell Biology 2022Quote: Transforming plasmids were linearized by digestion with either ScaI-HF® or AseI restriction enzymes (NEB), which cut within the ampicillin resistance gene ...
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bioRxiv - Biophysics 2022Quote: ... 2.5 kilo base pair DNA tethers were PCR-amplified from the pUC19 plasmid (New England Biolabs) with a 5’-biotinylated primer on one side and a 5’-phosphoprimer on the other side ...
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bioRxiv - Developmental Biology 2022Quote: ... into the linearized PacI-AvrII pUASt-attB plasmid using ligation T4 strategy (New England Biolabs, Inc.).
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bioRxiv - Microbiology 2021Quote: ... the entire pGE450 plasmid was amplified by inverse PCR using Q5 High Fidelity DNA polymerase (NEB) with oligonucleotide TGD4981 and TGD4982 ...
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bioRxiv - Microbiology 2021Quote: ... the entire pGE190 plasmid was amplified by inverse PCR using Q5 High Fidelity DNA polymerase (NEB) with oligonucleotides TGD4006 and TGD5162 ...
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bioRxiv - Microbiology 2021Quote: Complementation plasmid pMQ70_lasA was generated using the NEBuilder HiFi DNA assembly cloning kit (New England BioLabs). P ...
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bioRxiv - Neuroscience 2020Quote: cDNA of tau 151-254 was inserted into a pMCSG17 plasmid using HiFi assembly (NEB #E2621S). The resulting vector encoded a protein consisting of a hexahistidine tag followed tobacco etch virus (TEV ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmids were isolated from positive colonies using Monarch Miniprep kit (New England Biolabs, Ipswich, MA, US) and then FastDigest XbaI or XhoI separately (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The 15.6-Kb ANEp8_Cas9_LIC1 plasmid was initially digested with the NotI restriction enzyme (New England Biolabs) to liberate a 5.3-Kb fragment containing the AMA1 cassette (purified by gel extraction using an Invitrogen PureLink Quick Gel Extraction Kit ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids constructed in this study were obtained by using NEBuilder HiFi DNA assembly (New England Biolabs) method ...
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bioRxiv - Genomics 2022Quote: ... and ligated with the aforementioned triple-digested plasmid pool using T4 DNA ligase (New England Biolabs) at 16°C overnight with a insert:vector molarity ratio of 20:1 ...
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bioRxiv - Cancer Biology 2022Quote: ... was sub-cloned via restriction digest of the plasmid with NsiI-HF (NEB, Cat. No. R3127) and SnaBI (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmid pl21 (TriAltus) was truncated by digestion with restriction enzymes XbaI and XhoI (New England Biolabs). To complete the recombinant assembly reaction NEBuilder® HiFi DNA Assembly kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... or GFP plasmid respectively were digested with XhoI for 1hr (New England Biolabs, Ipswich, MA, USA) for linearization ...
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bioRxiv - Bioengineering 2019Quote: ... ligation products enclosing fragments of non-integrated transposon donor plasmid DNA were digested with DpnI (NEB) in 50 µl overnight ...
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bioRxiv - Synthetic Biology 2019Quote: ... and inserts and plasmid were ligated at a 1:1 molar ratio with Quick Ligase (NEB) for 20 min at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... a mini-CTX-lux (TcR) plasmid (38) was digested with EcoRI and BamHI (New England BioLabs). The promoter region of mexAB-oprM operon was amplified with EcoRI_PmexAB_Fw and BamHI_PmexAB_Rv primers ...
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bioRxiv - Zoology 2019Quote: ... The pCMV-BI empty plasmid was digested with DraIII-HF (Cat. Nr. R3510, New England Biolabs) and NcoI-HF (Cat ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were assembled using NEBuilder® HiFi DNA Assembly Master Mix following manufacturer’s protocol (NEB, MA). All plasmid sequences were confirmed by DNA sequencing (Eton Bioscience ...
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bioRxiv - Synthetic Biology 2019Quote: ... Expression plasmids were cloned using Gibson Assembly (NEB Gibson Assembly Master Mix, New England Biolabs #E2611) into a pET-28c plasmid backbone ...
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bioRxiv - Synthetic Biology 2021Quote: ... plasmid backbone DNA (1 μg) was digested using EcoRI-HF (20 units; R3101S, New England Biolabs) and SpeI-HF (20 units ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were constructed via isothermal DNA assembly (IDA; all enzymes and dNTPs from New England Biolabs). PCR products and plasmids were cleaned up using Qiagen kits and sequenced using Eurofins Genomics ...
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bioRxiv - Genetics 2020Quote: The pVG344 plasmid was cloned using the NEBuilder HIFI DNA Assembly Cloning Kit (New England Biolabs) to assemble four amplified fragments ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting plasmid library was then electroporated into electrocompetent NEB10β cells (New England Biolabs, Waltham, MA), which were then grown in liquid culture prior to purification of the plasmid library using a ZymoPure II Endotoxin-Free Midiprep kit (Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids with mutated Marcksl1a and Marcksl1b were generated using Q5® Site-Directed mutagenesis kit (NEB). shRNA containing vectors were constructed by ligation of shRNA sequence between the EcoRI and PmeI sites downstream of the U6 promoter of modified pEGFP-U6 vector 36 (a gift from Zuoshang Xu ...
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bioRxiv - Developmental Biology 2021Quote: ... and integrated into a BmtI and EcoRI-HF-digested pOEMGED plasmid via Gibson assembly method (NEB), with 1:3:3 plasmid to amplicon ratio ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids were generated by Gibson assembly of PCR fragments using the NEBuilder HiFi Master Mix (NEB). Fragments were created by PCR with the relevant primers (listed in Supplementary Table 2 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Linear plasmids were subjected to bisulfite conversion using the EpiMark Bisulfite Conversion Kit (New England Biolabs), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were purified using the Monarch PCR & DNA Cleanup Kit (New England BioLabs., Ipswich, MA, USA). Linearized plasmids ...
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bioRxiv - Genomics 2020Quote: ... The resulting linearised plasmid and double stranded oligonucleotides were ligated using Gibson Assembly Master Mix (NEB) as per manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Mutations into these plasmids were introduced using a Q5 site-directed mutagenesis kit (New England Biolabs).
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bioRxiv - Molecular Biology 2020Quote: ... and then inserted into pU6-2-gRNA plasmids using T4 DNA ligase (New England Biolabs # M0202S).
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bioRxiv - Microbiology 2021Quote: All plasmids generated in this study were created by Q5 Site-Directed Mutagenesis (New England Biolabs) of existing plasmids or HiFi Gibson Assembly (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... as per manufacturer recommendations and the assembled plasmids transformed into NEB5a cells (New England Biolabs; C3019). DNA sequencing was performed to verify the integrity of the construct.
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bioRxiv - Cell Biology 2021Quote: ... This was ligated into the plasmid pMT-FMR1-V5 (29) using the restriction enzymes XhoI (NEB) and PmeI (NEB) ...
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bioRxiv - Developmental Biology 2022Quote: ... The plasmid was linearized via EcoRV restriction enzyme digestion using EcoRV-HF (New England Biolabs #R3195S) before Gibson assembly.
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bioRxiv - Neuroscience 2022Quote: ... Plasmid and insert were then ligated overnight at 16 °C (T4 DNA ligase, New England Biolabs). After transformation into chemically competent E.coli and subsequent plasmid purification ...
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bioRxiv - Cancer Biology 2022Quote: All the plasmid constructs were generated using NEBuilder® HiFi DNA Assembly Master Mix (NEB, E2621L) as per the vendor’s instructions with some modifications ...
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bioRxiv - Cancer Biology 2022Quote: ... were cloned by using ClaI and EcoRI restriction enzymes into the pSNAPm plasmid (New England Biolabs), with the SNAP tag in the C-terminus of the insert ...
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bioRxiv - Cell Biology 2022Quote: ... and the PCR product was ligated into the plasmid using T4 DNA ligase (New England Biolabs), which yield pDRF1-GW AKAR3-EV.