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Citations for New England Biolabs :
1051 - 1100 of 2416 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Repair template plasmids were assembled using the NEBuilder Hifi DNA Assembly Kit (New England BioLabs) to incorporate homology arms into the loxP + Pmyo-2∷GFP∷unc-54 3’UTR + Prps-27∷neoR∷unc-54 3’UTR + loxP dual-marker selection cassette vector (provided by Dr ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids are based on pFB001(31) and constructed by User Cloning™(New England Biolabs) in a three-way ligation method ...
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bioRxiv - Cell Biology 2019Quote: Plasmids were constructed using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, MA). All plasmids used in this study are described in Supplementary Table 2.
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bioRxiv - Bioengineering 2019Quote: ... The 3.8 kb pRS413 vector backbone was PCR amplified from plasmid pRS413 (New England Biolabs) using primer pair RS413-Fw/RS413-Rev for all yeast assembly reactions ...
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bioRxiv - Genomics 2020Quote: ... We digested the Lenti-gRNA-Puro plasmid with BsmBI restriction enzyme (New England Biolabs, NEB) for 12 h at 55 °C ...
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bioRxiv - Genomics 2020Quote: ... We digested the Lenti-gRNA-Puro plasmid with BsmBI restriction enzyme (New England Biolabs, NEB) for 12 h at 55 °C ...
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bioRxiv - Cell Biology 2019Quote: ... and cloned into the pL4440 plasmid using the T4 DNA Ligase (New England Biolabs, M0202). The cloned plasmid was transformed to E ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli strain DH5α was used for plasmid construction while SHuffle T7 Express (New England Biolabs) 20 was used for cyclonal expression and library selections ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR products and digested plasmids were purified with the Monarch PCR & DNA Cleanup Kit (NEB). Plasmids were purified using the QIAprep Spin Miniprep Kit (QIAGEN).
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bioRxiv - Biophysics 2021Quote: A 2.5 kbp DNA fragment was PCR amplified from the pUC19 plasmid (New England Biolabs) with a double digoxigenin-labeled primer (Biomers GmbH ...
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bioRxiv - Neuroscience 2020Quote: ... The Ps2Ex3 pDR274 plasmid was linearized with HindIII-HF ® (NEB, Ipswich, Massachusetts, USA, R3104S), and then used as a template for synthesis of Ps2Ex3 sgRNA with the MAXIscript™ T7 Transcription Kit (Ambion ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids were transformed and grown in high efficiency DH5α competent cells (New England Biolabs C2987) before extraction by Miniprep (QIAGEN #27104) ...
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bioRxiv - Molecular Biology 2021Quote: ... and cloned into either BbsI-digested or Esp3I-digested plasmid by T4 DNA ligase (NEB) at 16°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... 30ug of transposon-containing genomic plasmid was digested with NotI-HF (New England Biolabs, USA) for 3 hours to remove the transposon body ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated from the pHH21-NA flag plasmids with Q5 Hot-Start PCR (NEB). This cDNA product was then cloned into the mammalian expression vector pCAGGS for transient transfection experiments as previously described [51].
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bioRxiv - Microbiology 2021Quote: The pCDNA3.1-hACE2-FLAG plasmid and a Q5 Site-Directed Mutagenesis Kit (New England Biolabs) were used according to the manufacturer’s specifications to generate recombinant plasmids that expressed hACE2 N-glycosylation mutants ...
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bioRxiv - Microbiology 2020Quote: ... The digested plasmid was then incubated twice with Antarctic Phosphatase (New England Biolabs, Ipswich, MA) to prevent self-ligation of cut plasmid ...
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bioRxiv - Cancer Biology 2020Quote: ... Plasmids were digested with BsmBI and BamHI in Cut Smart Buffer (New England BioLabs, Inc.) at 37°C for 1 hour and then analyzed by 1% agarose gel ...
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bioRxiv - Biophysics 2021Quote: ... plasmid containing the ctSTIM1 insert was transformed to BL21 competent cells (New England Biolabs #C25271) for pET28a plasmids ...
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bioRxiv - Biophysics 2020Quote: ... 2.5 kbp and 1.3 kbp DNA tethers were generated by PCR from pUC19 plasmid (NEB) with a double digoxigenin- or biotin-labeled primer on one side and a phosphoprimer on the other side ...
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bioRxiv - Neuroscience 2022Quote: ... The plasmids were used to amplify the UTRs with Phusion High-Fidelity DNA polymerase (NEB) using corresponding primers carrying SfiI and NheI restriction sites to allow the inserts between SV40 promoter and the reading frame of hRluc ...
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bioRxiv - Cell Biology 2022Quote: ... the same plasmid was linearised with ApaI and transcribed with SP6 RNA polymerase (both NEB). ‘Full length’ tert cDNA was amplified using primers FW ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant plasmid was generated by a NEBuilder® HiFi DNA Assembly (New England Biolabs) reaction ...
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bioRxiv - Genetics 2022Quote: ... We digested backbone plasmid BFA022425 with the restriction enzymes HpaI and BsmBI (New England Biolabs) to remove the backbone vector’s existing gRNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmids were constructed by Gibson Assembly using NEBuilder® HiFi DNA Assembly Master Mix (NEB) or ligation using T4 DNA ligase (NEB) ...
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bioRxiv - Genomics 2022Quote: ... the PCUP1-Sup35N-Aß42 plasmid was linearised by PCR (Q5 high-fidelity DNA polymerase, NEB) with primers that remove the WT Aß42 sequence (primers MS_03-04 ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasmids containing NHEJ or HR reporter cassettes were linearized by I-SceI restriction enzymes (NEB) and purified using GeneJET PCR purification kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... Any modifications to plasmid sequences were generated by Q5 site-directed mutagenesis (New England BioLabs) and confirmed by sequencing ...
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bioRxiv - Bioengineering 2020Quote: The pGRA-AP2IX1-V5 plasmid for AP2IX1 transient expression was created using Gibson assembly (NEB) from the pGRA-V567 ...
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bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... 750 ng of transcribed plasmids were then incubated in the CutSmart Buffer (New England Biolabs) containing appropriate restriction enzymes and 2 U of RNase H ...
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bioRxiv - Microbiology 2019Quote: ... The plasmid was cut and de-phosphorylated simultaneously using 1 U Shrimp Alkaline Phosphatase (NEB) and 20 U EcoRV-HF (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids are based on pFB001(50) and constructed by User Cloning™(New England Biolabs) in a three-way ligation method ...
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bioRxiv - Biochemistry 2019Quote: ... 4 μg of plasmid was treated with 1U Klenow Fragment (3’ to 5’ exo-, NEB) for 10 min at 37°C to fill any remaining gapped plasmid 12 ...
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bioRxiv - Molecular Biology 2020Quote: All restriction endonuclease digests of plasmids were performed according to manufacturer’s instructions (New England Biolabs).
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bioRxiv - Molecular Biology 2020Quote: ... This TRE-LSL plasmid was then opened up using restriction enzymes XbaI and EcroI (NEB). After which the remaining three inserts ...
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bioRxiv - Cell Biology 2020Quote: ... Digested plasmids were gel purified and ligated together using T4 DNA ligase (New England Biolabs). Plasmids were transfected into NEB5α bacteria and positive clones screened by PCR and verified by Sanger sequencing ...
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bioRxiv - Microbiology 2021Quote: ... The pGEMT amyE plasmid and the cat-gfp amplicon were both digested by KasI (NEB) and the two linear fragments with compatible 5’ overhangs were ligated together to obtain the PGEMT amyEup-cat-gfp-amyEdw plasmid ...
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bioRxiv - Biochemistry 2021Quote: Plasmids were constructed by NEBuilder HiFi DNA assembly (New England Biolabs, Frankfurt am Main, Germany) or by ligation using T4 DNA (New England Biolabs ...
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bioRxiv - Genomics 2019Quote: ... Inserts were cloned into the pGL4.23c plasmid using NEBuilder HiFi DNA Assembly Cloning Kit (NEB), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... the ends of the NcoI/BsrGI-digested plasmid were filled with T4 DNA polymerase (NEB) according to the manufacturer’s protocol and blunt ends were re-ligated ...
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bioRxiv - Neuroscience 2020Quote: ... The purified plasmid DNA was linearized via the restriction enzyme NheI (New England Biolabs (Genesearch), Arundel ...
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bioRxiv - Biochemistry 2021Quote: ... 0.5 μg of R-loop-containing plasmid was incubated with 0.5 U RNase H (NEB) in 75 mM KCl / 50 mM Tris-HCl pH 7.5 / 3 mM MgCl2 / 10 mM DTT in a 20 μL reaction volume and incubated at 37 °C for 30 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... plasmid DNA was treated using the EpiMark® Bisulfite Conversion Kit (E3318S, New England Biolabs). The protocol was modified to allow non-denaturing deamination ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA was cut by restriction digest using NotI or BspQI enzymes (New England Biolabs), respectively ...
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bioRxiv - Immunology 2020Quote: ... LeGO-iT2 and LeGO-iT2puro plasmids were cut by NotI-HF (New England Biolabs (NEB), USA ...
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bioRxiv - Immunology 2020Quote: ... LeGO-iT2 and LeGO-iT2puro plasmids were cut by NotI-HF (New England Biolabs (NEB), USA ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid vector was digested by restriction digestion using KpnI-HiFi and XbaI (New England BioLabs) at 37 °C for 2 hrs ...
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bioRxiv - Developmental Biology 2022Quote: ... and subcloned into pCAGGS_Myc plasmid using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). Individual ASO-targeted sequences were mutated by inverse PCR with PrimeSTAR GXL DNA polymerase (TaKaRa ...