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Citations for New England Biolabs :
1001 - 1050 of 2232 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... 750 ng of transcribed plasmids were then incubated in the CutSmart Buffer (New England Biolabs) containing appropriate restriction enzymes and 2 U of RNase H ...
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bioRxiv - Microbiology 2019Quote: ... The plasmid was cut and de-phosphorylated simultaneously using 1 U Shrimp Alkaline Phosphatase (NEB) and 20 U EcoRV-HF (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids are based on pFB001(50) and constructed by User Cloning™(New England Biolabs) in a three-way ligation method ...
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bioRxiv - Biochemistry 2019Quote: ... 4 μg of plasmid was treated with 1U Klenow Fragment (3’ to 5’ exo-, NEB) for 10 min at 37°C to fill any remaining gapped plasmid 12 ...
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bioRxiv - Molecular Biology 2020Quote: All restriction endonuclease digests of plasmids were performed according to manufacturer’s instructions (New England Biolabs).
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bioRxiv - Molecular Biology 2020Quote: ... This TRE-LSL plasmid was then opened up using restriction enzymes XbaI and EcroI (NEB). After which the remaining three inserts ...
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bioRxiv - Cell Biology 2020Quote: ... Digested plasmids were gel purified and ligated together using T4 DNA ligase (New England Biolabs). Plasmids were transfected into NEB5α bacteria and positive clones screened by PCR and verified by Sanger sequencing ...
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bioRxiv - Microbiology 2021Quote: ... The pGEMT amyE plasmid and the cat-gfp amplicon were both digested by KasI (NEB) and the two linear fragments with compatible 5’ overhangs were ligated together to obtain the PGEMT amyEup-cat-gfp-amyEdw plasmid ...
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bioRxiv - Biochemistry 2021Quote: Plasmids were constructed by NEBuilder HiFi DNA assembly (New England Biolabs, Frankfurt am Main, Germany) or by ligation using T4 DNA (New England Biolabs ...
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bioRxiv - Genomics 2019Quote: ... Inserts were cloned into the pGL4.23c plasmid using NEBuilder HiFi DNA Assembly Cloning Kit (NEB), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... the ends of the NcoI/BsrGI-digested plasmid were filled with T4 DNA polymerase (NEB) according to the manufacturer’s protocol and blunt ends were re-ligated ...
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bioRxiv - Neuroscience 2020Quote: ... The purified plasmid DNA was linearized via the restriction enzyme NheI (New England Biolabs (Genesearch), Arundel ...
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bioRxiv - Biochemistry 2021Quote: ... 0.5 μg of R-loop-containing plasmid was incubated with 0.5 U RNase H (NEB) in 75 mM KCl / 50 mM Tris-HCl pH 7.5 / 3 mM MgCl2 / 10 mM DTT in a 20 μL reaction volume and incubated at 37 °C for 30 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... plasmid DNA was treated using the EpiMark® Bisulfite Conversion Kit (E3318S, New England Biolabs). The protocol was modified to allow non-denaturing deamination ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA was cut by restriction digest using NotI or BspQI enzymes (New England Biolabs), respectively ...
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bioRxiv - Immunology 2020Quote: ... LeGO-iT2 and LeGO-iT2puro plasmids were cut by NotI-HF (New England Biolabs (NEB), USA ...
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bioRxiv - Immunology 2020Quote: ... LeGO-iT2 and LeGO-iT2puro plasmids were cut by NotI-HF (New England Biolabs (NEB), USA ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid vector was digested by restriction digestion using KpnI-HiFi and XbaI (New England BioLabs) at 37 °C for 2 hrs ...
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bioRxiv - Developmental Biology 2022Quote: ... and subcloned into pCAGGS_Myc plasmid using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). Individual ASO-targeted sequences were mutated by inverse PCR with PrimeSTAR GXL DNA polymerase (TaKaRa ...
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bioRxiv - Molecular Biology 2022Quote: ... pooled plasmids were end-repaired using 1uL (5U) DNA Polymerase I Klenow Fragment (NEB M0210S) with 33 μM each dNTP and 1x NEB CutSmart buffer per 1000ng DNA pool ...
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bioRxiv - Cancer Biology 2022Quote: ... we PCR amplified a mCMV from a plasmid using primers XhoI_attL1_mCMV_FW and SalI_mCMV_RV followed by T7 ligation (NEB) of both PCR products ...
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bioRxiv - Biochemistry 2022Quote: ... wild-type CSF3R MIG plasmid and insert were digested with SfiI (New England Biolabs R0123S), ligated overnight with a 3:1 molar ratio ...
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bioRxiv - Genetics 2022Quote: ... then the insert was isolated by digesting approximately 10ug of plasmid with NotI (NEB, #R0189) for 2hrs at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Both the PCR product and the recipient plasmid were digested with XhoI and BamHI (NEB) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid pACYC184 (pACYCev; empty vector) was linearized by BamHI and HindIII (New England Biolabs). The gmhB locus ...
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bioRxiv - Microbiology 2022Quote: ... Open circular plasmid control was obtained by incubation with the Nt.BspQI nickase (New England Biolabs), and linear plasmid by cleavage with BamHI.
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bioRxiv - Neuroscience 2023Quote: ... and those involving AAV plasmids were transformed into NEB Stable competent cells (New England Biolabs).
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bioRxiv - Biophysics 2022Quote: Plasmids expressing stdMCP-fusion proteins were generated using the NEBuilder HiFi DNA Assembly kit (NEB). To obtain plasmids carrying an stdGFP-tagged stdMCP gene ...
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bioRxiv - Biophysics 2022Quote: The pcDNA3-HaloTag7-Sam68 plasmid was prepared using the NEBuilder HiFi DNA Assembly kit (NEB). The vector backbone and the HaloTag7 insert were amplified by PCR using the pcDNA3-HA-Sam68-WT vector (Addgene ...
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bioRxiv - Synthetic Biology 2023Quote: ... 25 µg of pRS193 lentiviral cloning backbone plasmid was digested by EcoRI-HF (NEB #R3101S) and XbaI (NEB #R0145 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The barcode plasmid libraries were used to transform T7 Express chemically competent cells (NEB #C2566I) to establish barcoded E ...
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bioRxiv - Microbiology 2023Quote: ... the pABA plasmid (from E. coli INV110) was methylated with Dam methylase (New England BioLabs), following the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: Miniprep plasmid DNA was digested with restriction enzyme KpnI following manufacturer’s instructions (New England Biolabs). After two hours at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmids encoding for GAF2 mutants were prepared by Q5 site-directed PCR mutagenesis (NEB) of pET28a-GAF2 using the primers listed in Table S1.
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bioRxiv - Bioengineering 2023Quote: ... The PCR-linearized plasmid was then circularized by the T4 ligase (NEB, Ipswich, MA, USA) to create pTargetF_araA ...
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bioRxiv - Biophysics 2023Quote: ... Calmodulin and myosin-5 expression plasmids were prepared from E.coli 5α cells (New England Biolabs), transformed with the each of plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... and ligated with the digested cutting plasmid with T4 DNA ligase (New England Biolabs; #M0202). The ligated fragments were transformed into DH5α E ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmids were edited via site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB, Cat #E0554).
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bioRxiv - Microbiology 2023Quote: ... Plasmids were constructed by Gibson Assembly using NEBuilder® HiFi DNA Assembly Master Mix (NEB) or ligation using T4 DNA ligase (NEB) ...
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bioRxiv - Zoology 2023Quote: ... The amplified fragment and UAS plasmid (pSL*attB:YFP:Gyp:UAS14i:Gyp:attB) (10) were ligated following EcoRI and XhoI (NEB) digestion and purification ...
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bioRxiv - Synthetic Biology 2023Quote: ... All enzymes used in this for plasmid construction were purchased from New England Biolabs (NEB). Primers were ordered from Oligomer Inc ...
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bioRxiv - Molecular Biology 2023Quote: ... The assembled plasmid was introduced into Escherichia coli strain NEB 5-alpha (New England Biolabs) by heat shocking ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmid pAM5825 were created by digesting pAM4950 with SwaI and performing a Gibson Assembly (NEB) between the linearized product and a cotA gBlocks Gene Fragment synthesized by Integrated DNA Technologies (IDT) ...
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bioRxiv - Cancer Biology 2022Quote: Mutations were introduced into RARα-encoding plasmids using Q5 Site-Directed Mutagenesis Kit (NEB E0554). Mutations were confirmed by sequencing of the resulting plasmids.
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bioRxiv - Microbiology 2023Quote: ... Mutagenesis plasmids were generated by the isothermal assembly method using the HiFi system (NEB, UK). In brief ...
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bioRxiv - Biochemistry 2023Quote: ... was cloned into pMIG-GFP plasmid by restriction digestion method using Bgl2 and EcoR1 (NEB). Primers used for cloning (Forward primer ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were amplified in chemically competent 5-alpha F’Iq Escherichia coli cells (New England Biolabs) after 42°C heat shock transformation and extracted using the GenElute HP Plasmid Miniprep Kit (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... plasmids were chemically transformed into DH5-alpha cells (cat no. C2987, New England Biolabs Inc.) and plated on agar plates containing appropriate antibiotics ...
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bioRxiv - Microbiology 2023Quote: ... the drop-out plasmid pHC8 was generated by Golden Gate Assembly kit (New England BioLabs), and the basic genetic parts are from Marburg collection (65) ...