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Citations for New England Biolabs :
51 - 100 of 1832 citations for 26S Proteasome Non ATPase Regulatory Subunit 10 PSMD10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... full-length CTPS1 and CTPS2 cDNAs were obtained by PCR as previously described [26] using the Q5® High-Fidelity DNA Polymerase (New England Biolabs). For CTPS1 ...
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bioRxiv - Immunology 2021Quote: ... Non-isotopic methylated RNA fragments were prepared using cold SAM (Biolabs, 0991410) and in vitro transcribed RNA fragments under similar conditions.
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bioRxiv - Genomics 2019Quote: ... Non-circularized molecules were degraded using Exonuclease III and Exonuclease I (NEB). Circularized FlcDNAs was fragmented by sonication using a Covaris S220 (4 min ...
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bioRxiv - Genomics 2021Quote: ... a non-templated dA tail was added using Exo(−) Klenow (NEB, #M0212S) to 3’-P presenting in the RNA that was dephosphorylated using T4 PNK (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... cells were quenched with a non-fluorescent bromothenylpteridine (BTP; New England Biolabs) at 2 µM final concentration ...
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bioRxiv - Genetics 2020Quote: ... non-crosslinked chromatin of 15,000 mESCs was digested using MNase (NEB M0247) for 5-15 minutes at 20°C ...
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bioRxiv - Immunology 2022Quote: ... The sgRNA templates were generated by PCR (26) and used for synthesis of sgRNAs using NEB HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, E2050S). The sgRNAs purified using MEGAclear Kit (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... GGGMcm3 (in association with the other Mcm2-7 subunits) was cleaved off the resin with 500 units of 7×His-TEV protease (NEB; rotation overnight at 4°C). The flow-through was collected and applied to 0.4 mL volume Ni-NTA Agarose resin (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... anti-glutaryl-lysine (Cell Signaling Technologies, non-commercial #14943MF and PTM-Biolabs #1151), anti-succinyl-lysine (Cell Signaling Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... anti-succinyl-lysine (Cell Signaling Technologies, non-commercial # 13599 and PTM-Biolabs #401), anti-malonyl-lysine (Cell Signaling Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... purified genomic DNA was digested with the non-palindromic restriction enzyme BssS1 (NEB) and the 4 base restriction enzyme Mse1 (NEB ...
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bioRxiv - Plant Biology 2023Quote: ... Preparation of RNA library (non-directional 250∼300 bp insert cDNA library (NEB)) and transcriptome sequencing (Illumina Novaseq 6000 paired-end 150 ...
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bioRxiv - Molecular Biology 2023Quote: ... Non-soluble chromatin pellet was washed in 100 µL 1x MNase buffer (NEB), centrifuged and digested (2 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Non-binding mutant F211A was generated by Q5 site-directed mutagenesis (New England Biolabs)
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bioRxiv - Microbiology 2023Quote: ... followed by the second strand cDNA synthesis using dTTP for non-directional library (NEB Next® Ultra RNA Library Prep Kit for Illumina®) ...
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bioRxiv - Genomics 2021Quote: ... the NEBNext Ultra II Non-directional RNA Second Strand Synthesis Buffer and Reagents (NEB Biolabs) was used for the second strand synthesis.
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bioRxiv - Genomics 2021Quote: ... the NEBNext Ultra II Non-directional RNA Second Strand Synthesis Buffer and Reagents (NEB Biolabs) was used for the second strand synthesis.
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bioRxiv - Developmental Biology 2023Quote: ... CNCCs were re-suspended in DEPC-PBS containing 0.04% non-acetylated BSA (New England Biolabs). Cell viability was determined to be >95% by Trypan Blue staining ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.6) overnight at 37 °C in the presence of non-tritiated SAM (New England Biolabs). Samples were resolved via SDS-PAGE ...
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bioRxiv - Biophysics 2021Quote: ... all amplicons were digested with the non-palindromic restriction enzyme BsaI-HF (New England Biolabs, UK). The ligation of the fragments was carried out using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Biophysics 2021Quote: ... all amplicons were digested with the non-palindromic restriction enzyme BsaI-HFv2 (New England Biolabs, UK). The ligation of the DIG-handles and biotinylated DNA fragments was carried out overnight using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... in vitro synthesized and yeast non-poly(A) RNA were polyadenylated using E.coli polyA polymerase (NEB) at 37 °C for 15 min ...
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bioRxiv - Bioengineering 2019Quote: ... ligation products enclosing fragments of non-integrated transposon donor plasmid DNA were digested with DpnI (NEB) in 50 µl overnight ...
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bioRxiv - Microbiology 2021Quote: ... and the NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs, Germany). Afterwards ...
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bioRxiv - Evolutionary Biology 2021Quote: ... NEBNext Ultra II First Strand and Non-Directional RNA Second Strand Synthesis Module (NEB, Ipswich, Massachusetts) were used to convert RNA into cDNA ...
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bioRxiv - Genomics 2020Quote: ... NEBNext Ultra II Non-directional RNA Second strand synthesis module (New England BioLabs, Ipswich, MA, USA) enzyme mix was used for generating double strand DNA from first strand cDNA after incubation at 16°C for 1 hour.
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bioRxiv - Microbiology 2020Quote: ... followed by an NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs), to produce double-stranded cDNA from our RNA extracts ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein and non-incorporated nucleotides were removed using the Monarch RNA Clean up kit (NEB, T2040), before quantification by nanodrop.
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bioRxiv - Microbiology 2022Quote: ... and the NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs, Germany) were used ...
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bioRxiv - Molecular Biology 2023Quote: ... the non-mutated sequences were cleaved using Q5 KLD enzyme (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... 10 μL GlycoBuffer 2 (10 ×) (NEB), 10 μL 10% NP-40 (NEB) ...
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bioRxiv - Bioengineering 2023Quote: ... 10 μL 10% NP-40 (NEB), 10 μL PNGase F (NEB ...
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bioRxiv - Microbiology 2020Quote: ... and NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs, Part Number E6111L). Samples were further processed using the Kapa LTP Library Preparation Kit for Illumina Platforms (Kapa Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared using NEBNext Ultra II non-directional RNA Library Prep kit (New England BioLabs, # E7770S). Library quality and concentration was assessed with Labchip and qPCR ...
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bioRxiv - Biophysics 2022Quote: ... Non-fluorescent RNA was transcribed using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, #E2040S). Following in vitro transcription by either kit ...
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bioRxiv - Genomics 2023Quote: ... followed by second strand synthesis using NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB). Libraries were prepared according to the Nextera XT (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... and either stored at −20°C or 10 μg used for immunoblots with an Anti-IkBa antibody (New England Biolabs) (54).
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bioRxiv - Cell Biology 2020Quote: ... MNase (non-specific DNA digestion) used MNase buffer and 2 μL of enzyme (20 units/μL) PvuII (NEB), AluI (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... and immediately introduced into a second strand synthesis reaction using mRNA Non-Directional Second Strand Synthesis module (NEB). Double stranded DNA was then cleaned up using Ampure XP purification beads and eluted in 30 uL of nuclease-free water ...
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bioRxiv - Cancer Biology 2022Quote: ... Library preparation was performed with the NEBNext Ultra II Non-directional Synthesis Module (New England Biolabs, Ipswich, MA). Samples were sequenced on Illumina NextSeq High Output sequencer and aligned to combined genome from hg38 (human) ...
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bioRxiv - Molecular Biology 2019Quote: ... Non-overlapping forward and reverse primers containing the desired mutation were phosphorylated by polynucleotide kinase (New England Biolabs) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... and the NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs, Ipswich, MA, USA). Subsequently ...
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bioRxiv - Biophysics 2019Quote: ... Biotin- and digoxygenin-labeled handles were amplified with a non-proofreading Taq DNA polymerase (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biophysics 2019Quote: ... Biotin- and digoxygenin-labeled handles were amplified with a non-proofreading Taq DNA polymerase (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biochemistry 2023Quote: ... second strand synthesis was performed with the NEBNext Ultra II Non-Directional Second Strand Synthesis Module (NEB E6111S) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... non incorporated primers were removed using the Monarch PCR and DNA clean up kit (New England Biolabs, T1030), using a binding buffer ratio to DNA of 3:1 ...
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bioRxiv - Molecular Biology 2023Quote: ... and AAV9-based rep-cap plasmids incorporating individual heptameric peptides (EC1-10) (pACG2-[EC1-10], pACG2-QuadYF+TV-[EC1-10], and pACG9-[EC1-10]) were generated through site-directed mutagenesis (NEB Q5 site-directed mutagenesis kit ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mg of cell lysate was cleared with protein A magnetic beads to remove non-specific interactions (S1425S, NEB) and incubated overnight with anti-ZAP antibody (Proteintech 16820-1-AP ...
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bioRxiv - Microbiology 2019Quote: ... Each RNA was subjected to radioactive end-labeling or non-radioactive phosphorylation by T4 Polynucleotide Kinase (New England Biolabs). Each RNA was subjected to phosphorylation with equimolar concentrations of either 32P-γ-ATP or ATP ...
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bioRxiv - Cancer Biology 2019Quote: ... Biotin was removed from non-ligated restriction fragment ends by incubating 40μg of DNA with T4 DNA polymerase (NEB) for 4 hours at 20°C in the presence of dATP and dGTP ...