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Citations for New England Biolabs :
101 - 150 of 1933 citations for 26S Proteasome Non ATPase Regulatory Subunit 10 PSMD10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... Biotin- and digoxygenin-labeled handles were amplified with a non-proofreading Taq DNA polymerase (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2024Quote: ... non incorporated primers were removed using the Monarch PCR and DNA clean up kit (New England Biolabs, T1030), using a binding buffer ratio to DNA of 3:1 ...
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bioRxiv - Biochemistry 2023Quote: ... second strand synthesis was performed with the NEBNext Ultra II Non-Directional Second Strand Synthesis Module (NEB E6111S) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and AAV9-based rep-cap plasmids incorporating individual heptameric peptides (EC1-10) (pACG2-[EC1-10], pACG2-QuadYF+TV-[EC1-10], and pACG9-[EC1-10]) were generated through site-directed mutagenesis (NEB Q5 site-directed mutagenesis kit ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mg of cell lysate was cleared with protein A magnetic beads to remove non-specific interactions (S1425S, NEB) and incubated overnight with anti-ZAP antibody (Proteintech 16820-1-AP ...
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bioRxiv - Microbiology 2019Quote: ... Each RNA was subjected to radioactive end-labeling or non-radioactive phosphorylation by T4 Polynucleotide Kinase (New England Biolabs). Each RNA was subjected to phosphorylation with equimolar concentrations of either 32P-γ-ATP or ATP ...
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bioRxiv - Cancer Biology 2019Quote: ... Biotin was removed from non-ligated restriction fragment ends by incubating 40μg of DNA with T4 DNA polymerase (NEB) for 4 hours at 20°C in the presence of dATP and dGTP ...
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bioRxiv - Genomics 2021Quote: ... non integrated plasmids were cut by 2 h digestion at 37 °C with I-CeuI restriction enzyme (NEB, R0699S) in a total volume of 70 ul followed by 20 minutes heat inactivation at 65 °C ...
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bioRxiv - Biophysics 2020Quote: The bare (non-methylated) PCR constructs were also methylated enzymatically by CpG methyltransferase M.SssI (New England Biolabs, Ipswich, MA). To ensure complete methylation ...
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bioRxiv - Developmental Biology 2022Quote: ... truSeq-barcoded RNAseq libraries were generated with the Ultra II RNA Library Prep Kit (non-directional) (New England Biolabs). Each library was quantified with a Qubit 2.0 (dsDNA HS kit ...
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bioRxiv - Genomics 2020Quote: A modified non-directional NEBNext Ultra II First and Second Strand (#E7771 and #E6111, New England Biolabs, Ipswich, MA) protocol was used to generate long fragments of double-stranded cDNA as input material for the Nextera DNA Flex Enrichment with respiratory virus panel ...
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bioRxiv - Biochemistry 2021Quote: To generate circular DNA with a single nick phagemid DNA (~2,6 μg) with or without non-denaturing bisulfite treatment was treated with 20 Units of Nt.BstNBI (New England Biolabs) for 1 hour at 37°C in 50 μl of 1X NEBuffer 3.1 followed by deactivation at 70°C for twenty minutes.
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bioRxiv - Microbiology 2022Quote: ... Deglycosylation of purified protein was performed in non-denaturing conditions according to manufacturer’s protocol (Protein Deglycosylation Mix II, NEB).
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg non-methylated NEAT1_1 IVT product was radio-labelled with radioactive labelling mix (1 µL 10x PNK buffer, NEB, 1 µL NEAT1_1 IVT product ...
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bioRxiv - Molecular Biology 2023Quote: For DNA-RNA hybrid IP (DRIP) non-crosslinked lysates were incubated (1 h, 37°C) with 10U RNaseH (NEB) prior to immunoselection ...
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bioRxiv - Microbiology 2023Quote: ... NEBNext® UltraTM II Non-Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., MA, USA) was used for the remaining steps of library construction ...
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bioRxiv - Microbiology 2023Quote: ... Non-β-lactamase genes used as controls were cloned into the pBAD18 using the Gibson Assembly Cloning Kit (NEB). pBAD (RBS)_fwd/rev primer pairs were used to amplify the plasmid backbone ...
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bioRxiv - Genetics 2019Quote: ... and non-directional high-throughput sequencing libraries were prepared using NEBNext Ultra DNA Library Prep Kit (New England Biolabs, E7370). Libraries were processed on the Illumina HiSeq platform for single-end 50-nt sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... Second-strand cDNA synthesis was performed using NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs) and amplified by in vitro transcription (HiScribe ...
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bioRxiv - Microbiology 2020Quote: ... double stranded DNA (dsDNA) was generated by use of NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB), purified using PureLink PCR Micro Kit (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2021Quote: ... and the NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs, Ipswich, MA, USA). After conversion into cDNA ...
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bioRxiv - Genetics 2022Quote: ... L1 elements were then reconstructed by combination of non-mutated fragments from different clones using restriction enzymes (New England Biolabs) cutting within the L1 sequence.
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bioRxiv - Genomics 2019Quote: ... The second strand synthesis reaction mix was assembled as follows (NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module: E6111S, NEB): 20 µl of first strand cDNA ...
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bioRxiv - Molecular Biology 2019Quote: 100µM of the synthesized phosphopeptides (Aapptec) or non-phosphorylated peptides were incubated with 350units of recombinant GSK3β (New England Biolabs) and cold kinase buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The tested interference substrates of either target strand (TS) or non-target strand (NTS) were 5′-radiolabeled with T4 PNK (NEB) in the presence of gamma 32P-ATP ...
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bioRxiv - Molecular Biology 2021Quote: ... Non-binding double mutant R1226L/W1289A and non-binding single mutant W1289A were generated by Q5 site-directed mutagenesis (New England Biolabs). Constructs were sequence verified.
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bioRxiv - Genomics 2021Quote: ... The isolated mRNA was then used to prepare sequencing libraries using NEBNext Ultra II Non-directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (Index sets 1 and 2) (NEB). Next ...
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bioRxiv - Molecular Biology 2022Quote: ... The products were analyzed by electrophoresis in a non-denaturing 12% polyacrylamide gel against a 5’-labeled Low Molecular Weight DNA Ladder (New England Biolabs) and quantitated with a phosphorimager (Typhoon FLA 9500 ...
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bioRxiv - Molecular Biology 2022Quote: ... and analyzed by electrophoresis in a non-denaturing 12% polyacrylamide gel with 5’-labeled Low Molecular Weight DNA Ladder (New England Biolabs) run in a parallel lane ...
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bioRxiv - Plant Biology 2022Quote: ... by PCR based site-directed mutagenesis (SDM) using non-overlapping primers listed in Supplementary Table S1 and Phusion High-Fidelity DNA Polymerase (New England Biolabs). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids in which multiple cdk5 phosphorylation sites in the DCX C-terminus were replaced with non-phosphorylatable or phosphomimetic residues were cloned by Gibson assembly (New England Biolabs) of a synthetic DNA fragment carrying these changes (synthesized by Twist Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... Part Number 18080044) and NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs, Part Number E6111L) as per the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... The gene fragments were individually cloned into sensor plasmid backbones identical to those used in the previous identification of non-functional TetR(B) mutants following the manufacturers protocol for Gibson Assembly (New England Biolabs). The newly constructed plasmids carrying each of the combined mutants were then transformed into E ...
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bioRxiv - Molecular Biology 2022Quote: ... and then to double-stranded cDNA using the NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs). The double-stranded cDNA was then purified and concentrated using Agencourt Ampure XP beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... were made by non-overlapping mutagenesis following the procedure described in the Q5 site-directed mutagenesis kit (New England Biolabs), using the pSS393 as template and divergent primers ...
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bioRxiv - Cell Biology 2022Quote: ... Non-directional sequencing libraries were completed with the “NEBNext Ultra II FS DNA Library Prep Kit for Illumina” (NEB #E7805) and subsequently analyzed on an Illumina NextSeq 550 with v2.5 reagent kits following manufacturer’s protocols.
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bioRxiv - Molecular Biology 2023Quote: ... Non-IRES mRNAs were capped the 3’-O-Me-m7G(5’)ppp(5’)G anti-reverse cap analog (NEB # S1411L). IRES mRNAs were capped with the A(5’)ppp(5’)G cap analog (NEB # S1406L) ...
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bioRxiv - Immunology 2023Quote: RNA of the carriers and non-carriers were constructed into transcriptome libraries using NEBNext Single Cell/Low Input RNA Library Prep Kit (New England BioLabs) and sequenced using NextSeq2000 (Illumina ...
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bioRxiv - Genomics 2024Quote: ... TruSeq-barcoded RNAseq libraries were generated with the NEBNext Ultra II Non-Directional RNA Library Prep Kit (New England Biolabs). Each library was quantified with a Qubit (dsDNA HS kit ...
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bioRxiv - Microbiology 2024Quote: ... the complete reaction was used for 2nd strand synthesis with the NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs), followed by DNA fragmentation with a Covaris M220 and library preparation with a GeneRead DNA Library L Core kit (QIAGEN ...
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bioRxiv - Microbiology 2024Quote: ... SINV-REP construct derived from pToto64 clone encoding SINV non-structural proteins and a reporter luciferase tag was linearized using SacI (New England Biolabs), followed by in vitro transcription using SP6 RNA polymerase (New England Biolabs) ...
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bioRxiv - Genomics 2019Quote: ... followed by adding 10 µL PNK (10 U/µL, NEB). The mixture was incubated for 30 min at 37 °C with shaking at 800 rpm ...
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bioRxiv - Genetics 2024Quote: ... 10 µL 10 mg/mL BSA (NEB cat no. B9000s), 840 μL DNA grade H2O (Invitrogen Waltham ...
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bioRxiv - Genomics 2021Quote: 10 μg genomic DNA from transfected mESCs were digested using 10 μl 10 U/μL NlaIII (NEB, #R0125S) in a 50 μL final volume for 3 hours at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer, 0.5 μl 10 μM NEB index primer ...
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bioRxiv - Molecular Biology 2021Quote: ... in 50 μl of total reaction volume (10 μl 5X KAPA buffer, 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer ...
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bioRxiv - Molecular Biology 2022Quote: ... in 50 μl of total reaction volume (10 μl 5X KAPA buffer, 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer, 0.5 μl 10 μM NEB index primer ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer, 0.5 μl 10 μM NEB index primer ...
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bioRxiv - Molecular Biology 2022Quote: ... in 50 μl of total reaction volume (10 μl 5X KAPA buffer, 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer ...