Labshake search
Citations for New England Biolabs :
8551 - 8600 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... The purified product was used as template for dsRNA synthesis using HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs) with subsequent purification with MEGAclear™ Transcription Clean-Up Kit (Ambion) ...
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bioRxiv - Genetics 2023Quote: ... Then the sequencing library was generated by using the NEBNext® Ultra II DNA Library Prep Kit (New England Biolabs; E7103S) and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Index Primers Set 1 ...
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bioRxiv - Developmental Biology 2023Quote: ... using Custom Nextera PCR primer and the NEBNext High-Fidelity 2x PCR Master Mix (Nextera DNA Library Preparation Kit (New England BioLabs, E7530L) and purified with the Qiagen MinElute Kit (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... pCS2-dKif6 (1-501)-EGFP was generated from the pCS2-dKif6-EGFP plasmid by Gibson assembly (NEB HiFi DNA Assembly Kit) using a GeneArt gene fragment encoding amino acids 1-501 (Thermo Fisher).
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bioRxiv - Immunology 2023Quote: ... Three independent sequencing libraries for each genotype were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA). Illumina NovaSeq 6000 was used for sequencing ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: 500 ng of RNA was processed using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB) with prior enrichment of mRNA by using Oligo dT beads from the NEBNext Poly(A ...
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bioRxiv - Genomics 2023Quote: ... E7490) and NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Inc., Cat. No. E7420). All libraries were dual-indexed using 12 cycles of PCR amplification using NEBNext® Multiplex Oligos for Illumina® Dual Index Primer Set (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... DNA was used to prepare a short reads library using NEBNext Ultra II DNA library preparation kit for Illumina (New England Biolabs, England) and sequenced on Illumina HiSeq X instrument (Illumina Inc. ...
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bioRxiv - Genomics 2023Quote: ... 125 ng of fragmented DNA was used to prepare each DNA library following the NEBNext Ultra II library prep kit for Illumina (NEB, Inc.) until the loop-adaptor ligation step ...
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bioRxiv - Biophysics 2023Quote: ... PUS1D134A lacking the N-terminal HIS-tag was subcloned into expression vector pET21d (EMD Biosciences) using a Gibson Assembly cloning kit and protocol (NEB # E5510S) and sequence verified ...
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bioRxiv - Developmental Biology 2023Quote: ... was combined with either MEP-1 or Mi-2 PCR-amplified coding sequences in a Gibson assembly reaction using NEBuilder® HiFi DNA Assembly kit (NEB) following manufacturers protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... All mRNAs were transcribed at 30°C for 2 hrs using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040S) and were co-transcriptionally capped (8:1 cap analog to GTP for ∼90% capping efficiency ...
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bioRxiv - Genomics 2023Quote: The template genomic DNA was fragmented by sonication to an insert size of 350 bp using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample and sequenced using an Illumina Hiseq 2500 PE150.
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bioRxiv - Genomics 2022Quote: ... Library preparation on samples was conducted using manufacturer’s protocols for NEBNext Ultra II Dna Library Prep Kit (New England BioLabs, CAT#E7645) and NEB Multiplex Dual Index oligos (New England BioLabs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were created using 1000ng of total RNA with the NEBNext Ultra 2 Directional RNA Library Kit following the Poly(A) mRNA Magnetic Isolation module (NEB #E7490). Libraries were sent to Novogene for Illumina Hi-Seq ...
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bioRxiv - Genomics 2023Quote: Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, cat. No E7645S and E7335S) and amplified using 14 PCR cycles following the manufacturer’s instructions with slight modifications as previously described by us in detail elsewhere25 ...
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bioRxiv - Developmental Biology 2023Quote: ... This plasmid was modified to include a C-terminal V5 tag using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, #E0554S). For arnt1-myc ...
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bioRxiv - Biochemistry 2023Quote: ... A linear expression cassette of pD912-PGAP-Hp AMPD-His10 was assembled using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520S). Primers were designed using the NEBuilder Assembly Tool ...
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bioRxiv - Genetics 2023Quote: ... cDNA and sequencing libraries were generated from 500 ng of fresh RNA samples with 10 cycles of PCR with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #7760). After quality checking using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was normalized for cDNA conversion using ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, Massachusetts, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 500 ng of total RNA was used as a template for cDNA synthesis with the LunaScript® RT SuperMix Kit (#E3010, New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... using a minimum of 20 bp overlapping regions between DNA fragments with custom made kit Plasmid selection and verification after the transformation were checked using the OneTaq® 2X Master Mix with Standard Buffer (NEB). Plasmids were purified using the Qiagen plasmid purification kit according to manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... was modified to introduce BsaI cut site and single sgRNAs were cloned into it by using Golden Gate Assembly kit (BsmBI-v2, New England Biolabs #E1602). All single sgRNAs used in this study are listed in Supplementary Table 5 ...
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bioRxiv - Cell Biology 2022Quote: ... Library preparation was done according to NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (#E7760L, New England Biolabs) including polyA enrichment and addition the of ERCC RNA spike-ins ...
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bioRxiv - Microbiology 2023Quote: High molecular weight DNA was extracted from isolate OI25 and its revertants using the Monarch® HMW DNA Extraction Kit for Tissue (New England Biolabs) according to the manufacturer’s protocol with the addition of 50 μg/ml lysozyme (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The 2X FLAG sequence was introduced via PCR as an oligonucleotide primer along with a reverse primer that produced the 3’ CNA1 UTR and cloned by use of the Gibson Assembly Cloning Kit (NEB #E5510S). The identity of the vector pCnat-CNA1-2X FLAG was also confirmed by sequencing.
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bioRxiv - Microbiology 2023Quote: ... Sequence libraries were prepared with the NEBNext Ultra II FS DNA Library Prep 136 kit (New England Biolabs GmbH, Frankfurt, Germany) and sequenced with the NovaSeq6000 (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was used to prepare poly-A selected directional libraries using the NEB Next Ultra Directional RNA Library Prep Kit (New England BioLabs, USA) and libraries were sequenced utilizing the Illumina Hiseq2000 platform at the sequencing core facility of the Max Planck Institute of Immunology and epigenetics (Freiburg ...
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bioRxiv - Molecular Biology 2023Quote: ... peptide sequences specific to an aMino sdAb were generated using the Ph.D.™-C7C Phage Display Peptide Library Kit (New England BioLabs, E8120S), a combinatorial library consisting of randomized display peptides with a disulphide constrained loop (AC-XXXXXXX-CGGGS ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... The annealed inserts were digested with EcoRI/AgeI for PLKO.1 and BsmBI for LentiCRISPRv2 and ligated using the Quick Ligation Kit (NEB M2200S).
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bioRxiv - Genetics 2023Quote: ... Index primers set 1 and 2 from the NEBNext Multiplex Oligos for Illumina kit (New England Biolabs, E7335S/L E7500S/L) were incorporated using Herculase II Fusion Polymerase Kit (Agilent ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... prior to mRNA library preparation using the Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs). Libraries were sequenced on an Illumina HiSeq 2500 V4 system ...
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bioRxiv - Molecular Biology 2023Quote: ... In vitro mutagenesis was carried out with the Q5 site-directed mutagenesis kit (Cat. No. E0554S, New England Biolabs, Ipswich, MA). Primers specific for the desired mutations were designed with the NEBaseChanger on the NEB website ...
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bioRxiv - Plant Biology 2023Quote: ... DNA libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs) and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs).
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA was quality checked using Bioanalyser (RIN>9) and libraries generated using NEBNext Ultra II Library Prep Kit (NEB, E7770) as per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: EM-seq libraries were prepared from genomic DNA as previously (78) using the NEB Enzymatic Methyl-seq kit (New England BioLabs; P7120L). In brief ...
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bioRxiv - Genomics 2024Quote: ... The directional RNA-seq libraries were constructed using the NEBNext Ultra II Directional RNA Library prep for Illumina kit (NEB, E7760L) utilising the NEBNext Poly(A ...
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bioRxiv - Physiology 2024Quote: ... High-quality RNA samples were used to construct sequencing libraries with NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA), and the libraries were sequenced using Illumina HiSeq X ten/NovaSeq 6000 system (2 × 150 bp read length ...
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bioRxiv - Systems Biology 2024Quote: ... Approximately 100 ng of each DNA sample was used to create Illumina sequencing libraries using a NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs (NEB), E7805S) ...
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bioRxiv - Cell Biology 2024Quote: ... Purified RNAs were used for Illumina RNA-seq library preparation with NEBNext Ultra II Directional RNA Library Prep Kit (NEB, E7765), and a minimum of 20 million raw reads were obtained ...
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bioRxiv - Synthetic Biology 2024Quote: ... Purification of PCR products for downstream applications was accomplished using either phenol/chloroform ethanol precipitation or the Monarch® PCR & DNA Cleanup Kit (New England Biolabs) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from 107 hybridoma cells using the MonarchTM total RNA extraction kit (New England BioLabs, Ipswich, MA, USA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from three pooled killing assay spots (as detailed in the previous section) utilizing the Monarch® Genomic DNA Purification Kit (NEB). Quantification of genomic DNA copies was targeted at the kdpAB gene and carried out with the Naica® Crystal Digital PCR System using Sapphire chips (Stilla Technologies).
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bioRxiv - Neuroscience 2023Quote: ... was used to construct sequencing libraries using the NEBNext Ultra II DNA library preparation kit for Illumina (New England Biolabs; #E7760). Libraries were multiplexed using NEBNext Multiplex Oligos for Illumina (dual index primers ...
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bioRxiv - Microbiology 2023Quote: ... The NGS library preparation was carried out using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB, USA), which performs fragmentation ...
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bioRxiv - Molecular Biology 2023Quote: ... adaptor ligation and PCR indexing was performed on the denatured amplicons using NEB Next Ultra II DNA library prep kit for Illumina (New England Biolabs, UK). The resulting FASTQ files from RNP treated samples for each of the off target amplicons were analysed for indels through CRISPResso2 webtool [45] by comparing them with untreated samples.
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bioRxiv - Immunology 2023Quote: ... Base editors were cloned into the IVT template vector using NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, #E5520S) (Extended Data Table 5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting fragment was then inserted into XhoI and NdeI digested pET21a vector by NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520). As a result ...
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bioRxiv - Molecular Biology 2023Quote: ... were grown out in LB medium with 50 µg/mL Kanamycin and then pelleted before plasmids were isolated using the Monarch Plasmid Miniprep Kit (NEB, T1010L). Plasmids were sequenced (Sanger ...