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Citations for New England Biolabs :
8701 - 8750 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Nuclear RNA-seq libraries were prepared from DNA-free nuclear RNA using NEBNext® UltraTM II Directional RNA Library Prep Kit (NEB) after first depleting rRNA using the NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) ...
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bioRxiv - Neuroscience 2024Quote: ... was used to construct sequencing libraries using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs, NEB). Libraries were multiplexed using NEBNext Multiplex Oligos for Illumina (dual index primers ...
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bioRxiv - Neuroscience 2024Quote: ... 5 ng of ChIP and Input DNA was used to generate ChIP-seq libraries using the NEBNext Ultra DNA library prep kit for Illumina (New England Biolabs, NEB).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Reverse transcription (1 µg total RNA or 500 ng at lower concentrations) was performed using the ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560L), resulting in cDNA concentrations of 50 and 25 ng/µL ...
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bioRxiv - Microbiology 2020Quote: ... bacilliformis total RNA (for each 25 µl reaction) collected from various conditions using the Luna Universal One-Step RT-qPCR kit (New England BioLabs; Ipswich, MA) according to the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids from non-motile isolates with straightened flagella were purified using the NEB monarch DNA mini prep kit (NEB, Ipswich, MA) and sequenced by Sanger sequencing using the primers (5-GGCACGAATTCCGAGCTGACGACCGTTCAG-3’ ...
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bioRxiv - Plant Biology 2020Quote: ... All mutated YFP-CESA6 constructs used the verified YFP-CESA6 plasmid as the template and were obtained by Q5 Site Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA) with primers listed in Supplement Table1 ...
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bioRxiv - Plant Biology 2020Quote: ... Libraries for next generation sequencing were generated using the NEBNext Ultra II gDNA prep kit with the NEBNext Multiplex Oligos for Illumina (New England Biolabs, Ipswich, U.S.A.). Prior to library generation ...
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bioRxiv - Cancer Biology 2021Quote: ... ChIP-seq libraries were prepared from 3-5ng ChIPed DNA using NEBNext® Ultra™ II DNA Library Prep Kit (NEB, E7645S), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The resulting polyA-selected RNA was used to prepare RNA-seq libraries using the NEBNext® Ultra(tm) II Directional RNA Library Prep Kit for Illumina® (cat. #E7600, New England Biolabs), NEBNext® Multiplex Oligos for Illumina® Dual Index Primers Set 1 (cat ...
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bioRxiv - Cancer Biology 2021Quote: ... A 3’ 3x FLAG tag was then inserted by site directed mutagenesis using a Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) and the primers PPARGC1A_FLAG_SDM_F and PPARGC1A_FLAG_SDM_R (see Supplementary Table 1) ...
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bioRxiv - Microbiology 2020Quote: Stranded RNA libraries were prepared from poly(A)-selected RNA using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced using a NextSeq 550 ...
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bioRxiv - Microbiology 2020Quote: ... The T7-3M-eGFP-5M minigenome was made by replacing the Renilla luciferase gene in the T7-3M-Luc-5M minigenome with an eGFP gene through assembly of PCR fragments using the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs, MA, USA). A modified version of this minigenome ...
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bioRxiv - Cell Biology 2020Quote: ... as per the manufacturer’s protocol and reverse-transcribed to cDNA using a Protoscript first strand synthesis kit (New England Biolabs, cat. no. E6560S). Quantitative PCR was performed using SYBR Green (Biorad ...
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bioRxiv - Cell Biology 2020Quote: ... The targeting sequence was inserted into the sgRNA cassette of pDD162 using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs, Inc.), resulting in identical plasmids (pTS11 and pTS12).
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bioRxiv - Cell Biology 2020Quote: ... 3-4-points were induced in the miR-29a seed binding site using the Q5 Site Directed Mutagenesis Kit (NEB, Cat#E0554S). 20 ng of recombinant dual-luciferase plasmid and 6 pmol of either miR-29a mimic or scrambled control were mixed with 100 μL Opti-MEM containing 1 μL lipofectamine RNAiMax (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... using the LTP library preparation kit and employing NEBNext multiplex oligos for Illumina index primer pairs set 1 (New England Biolabs, Ipswich, Massachusetts). Sequencing was performed using a NextSeq 500/550 mid-output v2.5 300 cycle cartridge (Illumina ...
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bioRxiv - Genetics 2021Quote: ... 1 μg of total RNA per sample was used as input for library preparation using the NEBNext Ultra Directional RNA Library Prep kit for Illumina (NEB, cat. # E7420) with the PolyA selection with the NEBNext Poly(A ...
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bioRxiv - Plant Biology 2021Quote: ... 1.0 μg total RNA (0.1-0.5 μg for ovules) was used to generate mRNA-seq libraries using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs, Cat# E7775). Sera-Mag Magnetic SpeedBeads (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA sample libraries were then prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645L) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified T7 PCR products were confirmed by sequencing and transcribed into dsRNA using the T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). The dsRNA produced was purified via ethanol precipitation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA-seq libraries were prepared from 150 ng total RNA using NEBNext® Ultra™ II Directional RNA library Prep Kit for Illumina (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA-seq libraries were prepared from 500 ng total RNA using the NEBNext® Ultra™ II directional RNA library Prep Kit for Illumina (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... barcoded libraries with a targeted insert size of 480 bp were prepared using the NEBNext Ultra II DNA Library Prep Kit (E7645L, New England Biolabs, Ipswich, MA) and sequenced on a HiSeq 2500 using a 2×125 bp paired-end protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Barcoded libraries with a mean insert size of 480 bp were prepared using the NEBNext Ultra II DNA Library Prep Kit (E7645L, New England Biolabs, Ipswich, MA) and sequenced on a HiSeq 2500 using a 2 x 125 bp paired-end protocol.
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bioRxiv - Neuroscience 2020Quote: ... was used in the preparation of sequencing libraries using the NEB Ultra II Directional RNA Library Prep Kit (NEB catalogue number E7760), following the polyA mRNA workflow ...
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bioRxiv - Genomics 2020Quote: ... to a final volume of 25 μl for Illumina library preparation using the NEBNext ultra II DNA library prep kit for Illumina (New England Biolabs, UK; E7645). Following 7 cycles of PCR enrichment ...
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bioRxiv - Microbiology 2022Quote: ... The sequencing library for total RNA-sequencing was prepared using NEB Next Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat# E7530). Paired-end ...
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bioRxiv - Plant Biology 2022Quote: ... Subsequent library preparation was performed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2022Quote: ... RAD-seq library preparation was performed with the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, Massachusetts, USA) with the PstI restriction enzyme ...
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bioRxiv - Genomics 2022Quote: Libraries were prepared using the NEBNext® Ultra DNA Library Preparation Kit for NovaSeq and NEBNext® Ultra II FS DNA Library Preparation Kit for MiSeq sequencing following the manufacturer’s recommendations (New England Biolabs, Ipswich, MA). For the validation experiments ...
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bioRxiv - Systems Biology 2022Quote: ... strand-specific total-transcriptome RNA sequencing libraries were prepared by incorporating dUTPs in the second-strand synthesis step with NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB). Finally ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNAs were subjected to sequencing library construction using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB, E7645L), and were deep sequenced on a NextSeq 550 platform using 40nt/40nt pair-ended mode ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA synthesis was performed from 1 µg total RNA using the ProtoScript® First Strand cDNA Synthesis Kit (New England Biolabs, NEB) with the provided random primer mix according to the suppliers’ instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... was used to remove rRNA from Escherichia coli total RNA and RNA-seq libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA E7760L). Libraries were recovered with AMPure XP beads A63880 (Beckman Coulter ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was generated from 1 μg of total RNA using ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, #E6560).
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bioRxiv - Molecular Biology 2020Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770S, New England Biolabs, Ipswich, MA). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... Mutants selected for cloning were also analysed via T7 endonuclease I-based (T7E1) heteroduplex assay according to the manufacturers protocol (EnGen® Mutation Detection Kit, NEB #E3321), and the length of digested and undigested fragments were visually compared by gel electrophoresis (Fig ...
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bioRxiv - Molecular Biology 2021Quote: ... Input and CHART-seq libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB) according to kit instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The final pool was run on an Agilent Bioanalyzer dsDNA High Sensitivity chip and quantified using NEBNext Library Quant Kit for Illumina (cat # E7630L, New England Biolabs, Ipswich, USA).”
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bioRxiv - Molecular Biology 2021Quote: ... mRNA-Seq libraries were prepared from total RNA using the NEBNext Ultra II Directional RNA-Seq Kit (NEW ENGLAND BioLabs, Inc., UK). The isolation of mRNA was performed using the Poly(A ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was checked on a fragment analyzer and 50 ng was used for library preparation with the NEBNext® Ultra™ II DNA Library Prep Kit (NEB) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Sonicated DNA was subjected to end-repair and adapter ligation using the NEBNext® Ultra™ II DNA Library Prep Kit (NEB) following the NEB protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Sequencing libraries were prepared by using 10 ng of purified DNA (averaged size 250-300 bp) with the NEBNext® Ultra™ II Library Prep Kit for Illumina (New England Biolabs) using the application note for “Low input ChIP-seq.” ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, New England BioLabs, Ipswich, MA). The library preps were started with 92-1000 ng of total RNA ...
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bioRxiv - Genomics 2019Quote: ... were used to PCR amplify both wild type and RRM3 Mt cDNA inserts for ligation into a pET28b vector containing an N-terminal 10xHis-SUMO tag using the Quick Ligation kit (NEB, Ipswich, MA). Plasmids containing the cDNA of interest were verified by Sanger sequencing prior to expression (Quintara Biosciences ...
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bioRxiv - Genomics 2020Quote: ... In vitro transcription was done using the HiScribe T7 High Yield RNA Synthesis Kit according to the manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA).
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bioRxiv - Genomics 2020Quote: ... a library without the enrichment method was also processed using NEBNext® Ultra™ II FS DNA Library Prep kit following the manufacturer’s recommendations (New England Biolabs, USA). The library preparation for D ...
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bioRxiv - Microbiology 2019Quote: ... and also quantified using NEB Luna Universal qPCR Master Mix and Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA) for qPCR and RT-qPCR test ...
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bioRxiv - Molecular Biology 2019Quote: M6A and m1A immunoprecipitationwere adapted from the protocols provided in the EpiMark® N6-Methyladenosine Enrichment Kit (NEB #E1610S, New England BioLabs Inc) and that previously described (28) ...