Labshake search
Citations for New England Biolabs :
8801 - 8850 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Libraries for next-generation sequencing (NGS) were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipwich, MA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Genes of interest were cloned into the pLenti CMVTRE3G eGFP Neo (w821-1) plasmid using a Gibson Assembly Master Mix kit (Cat# E2611S, New England Biolabs, Ipswich, MA). pLenti CMV rtTA3 Blast (w756-1 ...
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bioRxiv - Microbiology 2020Quote: ... native barcode ligation using the EXP-NBD103 barcode kit (Oxford Nanopore Technologies, Oxford, UK) and Blunt/TA Ligase master mix (New England Biolabs, Ipswich, MA) was performed as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Fragmentation and library construction of the resulting PCR products were performed using an NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... mRNA Magnetic Isolation Module (E7490S) and library preparation was completed using NEBNext® Ultra Directional RNA Library Prep Kit for Illumina® (E7420L, New England Biolabs), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the purified cDNA was PCR amplified and barcoded using ONT’s PCR Barcoding Expansion 1-12 (EXP-PBC001) or PCR Barcoding Expansion 1-96 (EXP-PBC096) kits with LongAmp Taq 2x Mastermix (NEB, Ipswich, MA).
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bioRxiv - Microbiology 2021Quote: ... were cloned into the pCR-Blunt-II vector using the PCR Blunt Topo kit according to the manufacturer’s instructions and transformed into 10-beta CaCl2-chemically competent bacteria (originally NEB, generated in-house). Following amplifications of colonies and purification of plasmids using a QiAprep Mini kit (Qiagen) ...
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bioRxiv - Systems Biology 2021Quote: ... RNA-seq libraries were constructed with poly(A) mRNA enrichment and rRNA depletion processes using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA). The indexed libraries were multiplex-sequenced in duplicate using two flow cells ...
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bioRxiv - Microbiology 2021Quote: ... where RNA metagenomic libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). Libraries were pair-end sequenced (2×150 bp ...
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bioRxiv - Plant Biology 2021Quote: ... mRNA was isolated from the total RNA with an NEB Next poly(A) mRNA Magnetic Isolation Module Kit (New England Biolabs, MA, USA). RNA-seq libraries were synthesized using an NEB Next Ultra RNA Library Prep Kit for Illumina and an NEB Next Adaptor for Illumina Kit (New England Biolabs ...
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bioRxiv - Plant Biology 2021Quote: ... The digestion products were run on agarose gel and purified with Monarch® DNA Gel Extraction Kit (New England Biolabs® Inc.). The asRNA genes were ligated into pLSU-1.1 with T4 DNA Ligase (New England Biolabs® Inc. ...
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bioRxiv - Plant Biology 2020Quote: ... Libraries were constructed from 100 ng and 1 μg total RNA using the NEBNext UltraTM Directional RNA Library Prep Kit for Illumina for use with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Hitchin, UK). mRNA was isolated ...
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bioRxiv - Plant Biology 2020Quote: ... Site-directed mutagenesis was performed to change Arg106 to a histidine (His) using Q5® Site-Directed Mutagenesis Kit (New England Biolabs Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... ATG-free versions of the truncated OpIE2 promoter sequence (tIE2 promoter with no ATG triplet in its sequence) were created by Q5 Site-Directed Mutagenesis (SDM) Kit (New England Biolabs-NEB, USA). Primers used to generate mutations were designed using the NEBaseChanger tool (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries for sequencing were made from high quality RNA that were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: ... Isolated mRNAs were reverse-transcribed into double stranded cDNA and subjected to sequencing library construction using the NEBNext Ultra™ II RNA Library Prep Kit for Illumina (NEB, E7770) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Site directed mutagenesis of the selectivity filter region of EhEUKCATB1 was performed using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Hitchin, UK). Primer sequences (SDM1_F-TGACCGGAGAcTCCTGGTCTG ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-seq libraries were prepared using 1 µg of RNA and the NEBNext Ultra™ II RNA Library Prep Kit (NEB, E7775) with rRNA depletion following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... The PCR products were cloned into the BsmBI-digested optimized sgRNA lentiviral expression vector LRG2.1T using a Gibson assembly kit (New England Biolabs; Cat. No. E2611). The LRG2.1T vector was derived from a lentiviral U6-sgRNA-EFS-GFP expression vector (Addgene ...
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bioRxiv - Cell Biology 2021Quote: ... mutant (GFP-FIP3-I738E-ABD) were engineered from the McCaffrey plasmids GFP-FIP3 and GFP-FIP3I738E using the Q5 Mutagenesis Kit (New England Biolabs, Ipswich, MA) following manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: A total of 1 μg of RNA was used for cDNA library construction at Novogene using an NEBNext Ultra 2 RNA Library Prep Kit for Illumina (New England Biolabs; Cat.# E7775) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid encoding for Spike G614 variant was generated using the pTwist EF1alpha nCoV-2019 S 2xStrep plasmid and the Q5 site-directed mutagenesis kit (New England BioLabs, Evry, France) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Genomic (g)DNA was extracted from muscle biopsies (from n=27 participants) and isolated using the Monarch kit for DNA isolation (New England Biolabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Libraries were prepared with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina according to the supplier recommendations (NEB). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Immunology 2022Quote: ... The sgRNA templates were generated by PCR (26) and used for synthesis of sgRNAs using NEB HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, E2050S). The sgRNAs purified using MEGAclear Kit (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... at −80 °C prior to RNA extraction and library preparation (performed by Novogene Company Limited with NEB Next® Ultra™ RNA Library Prep Kit (NEB)) prior to RNA sequencing (paired-end 150 bp ...
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bioRxiv - Cancer Biology 2022Quote: ... The digested constructs were then purified with Ampure XP Beads and then ligated with the Quick Ligation Kit (NEB, Cat. No. M2200S).
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bioRxiv - Genomics 2022Quote: ... NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® and NEBNext® UltraTM II DNA Library Prep Kit for Illumina® according to the manufacturer’s instructions (New England Biolabs). Paired-end 100 bp reads were generated.
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bioRxiv - Microbiology 2022Quote: ... Reverse-transcription polymerase chain reaction (RT-PCR) was performed using the Luna Universal Probe One-Step RT-qPCR kit (New England BioLabs; https://www.neb.ca). The 5 ‘ untranslated region (UTR ...
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bioRxiv - Microbiology 2022Quote: Complementary DNA (cDNA) libraries were constructed with the Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). cDNA concentrations were measured using a Qubit fluorometer with a dsDNA BR/HS kit (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was transcribed to full-length cDNA using the SMART-Seq253 protocol and RNA-sequencing libraries were generated using the NEBNext® Ultra™ II FS DNA library preparation kit (New England Biolabs) according the manufacturer’s protocol with DNA input below 100 ng ...
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bioRxiv - Neuroscience 2022Quote: The 16S rRNA V4 region was amplified using the following primers 515f-Y: GTGYCAGCMGCCGCGGTA and 806r-N: GGACTACNVGGGTWTCTAAT and The Q5® high-fidelity DNA polymerase kit (New England BioLabs, UK). 2μl of extracted DNA was added to a PCR reaction mix prepared by mixing a final concentration of 1X Q5 reaction buffer ...
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bioRxiv - Immunology 2022Quote: B cells were cultured for 48 hours before total RNA was extracted using Quick-RNA™ Microprep Kit (ZymoResearch) and mRNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation (New England BioLabs). Stranded Illumina libraries were prepared with Swift Rapid RNA Library Kit according to the manufacturer’s instructions (Swift Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... The pACYC184::attI1 recipient plasmid was created by assembling the attI1 sequence (from R388) into the pACYC184 plasmid backbone using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, United States). The PCR products required for the assembly were generated using the attI1_fw/attI1_rev and pACYC184_backbone_F/pACYC184_backbone_R primer pairs ...
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bioRxiv - Neuroscience 2022Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... 150 ng of RNA per sample was employed in the generation of libraries using the NEB Next Ultra Pure RNA library kit for Illumina (NEB, cat # E7770S) per manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... and a PCR-free library was obtained with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs). Approximately 200 million of 150 bases pair-end reads were generated using the HiSeq X System (Illumina) ...
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bioRxiv - Genetics 2022Quote: ... the truncated versions of cwr-1 and the histidine mutant plasmids were prepared using the Q5 Site-Directed Mutagenesis Kit (E0552S NEB, Ipswich, MA). Gibson assembly (E2611S NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... The library for RNA-Seq was prepared by using NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs, Ipswich, MA). During the second cDNA synthesis dUTP was incorporated to maintain strand specificity ...
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bioRxiv - Cell Biology 2022Quote: ... The sample preparation was performed according to the protocol “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (NEB #E7760S/L). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA libraries were constructed using the NEBNext® Ultra(tm) II Directional RNA Library Prep Kit (New England Biolabs, Inc., Massachusetts, USA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: One microgram of total RNA from each sample was subjected to cDNA library construction using a NEBNext® Ultra non-directional RNA Library Prep Kit for Illumina® (New England Biolabs). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by column purification and on-column DNase digestion according to the TRIzol two-step protocol from the Monarch total RNA miniprep kit (NEB, cat. T2010S) or PureLink RNA mini kit (Ambion ...
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bioRxiv - Molecular Biology 2020Quote: ... The libraries of input fractions from Figure S8 were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for illumina (NEB).
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bioRxiv - Neuroscience 2020Quote: ... Sequencing libraries were prepared from a starting amount of 100ng total RNA using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina (E7370L, New England Biolabs) using ribosomal depletion as a selection method ...
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bioRxiv - Microbiology 2020Quote: ... 1-3 μg RNA was used to generate sequencing libraries with NEBNext Ultra™ RNA Library Prep Kit for Illumina (#E7530L, NEB, USA) with poly(A ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg total RNA with RIN value above 7 was used for library preparation by Genewiz using NEBNext® UltraTM RNA Library Prep Kit for Illumina® and NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Size selection of Adaptor-ligated DNA was then performed using AxyPrep Mag PCR Clean-up (Axygen) ...
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bioRxiv - Plant Biology 2021Quote: ... Library concentration was further quantified by qPCR using the NEBNext® Library Quant Kit for Illumina® (New England BioLabs®, Inc.). Libraries were sequenced on one lane of the Illumina® HiSeq4000 platform to generate 150-bp paired-end reads.
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bioRxiv - Molecular Biology 2020Quote: ... sgRNA target site mutation in mouse Thap1 cDNA was generated by Q5 Site-Directed Mutagenesis Kit (NEB, see Table S3 for primers). WT or Thap1-/-Brca1Δ11 MEFs (1 × 106 ...