Labshake search
Citations for New England Biolabs :
8501 - 8550 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: RNA was used as template to detect and quantify viral genomes by duplex reverse transcriptase (RT) quantitative polymerase chain reaction (RT-qPCR) using a Luna Universal Probe one-step RT-qPCR kit (New England Biolabs, E3006E). SARS-CoV-2-specific RNAs were detected by targeting the ORF1ab gene using the following set of primers and probes ...
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bioRxiv - Microbiology 2023Quote: ... the strand-specific cDNA libraries were constructed with a NEB Next Ultra II Directional RNA library preparation kit for Illumina and the NEB Next Multiplex Oligos for Illumina (96) (New England BioLabs, Germany). To assess quality and size of the libraries ...
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bioRxiv - Physiology 2023Quote: ... which were then used to replace the corresponding regions in the wildtype plasmids with NEBuilder® HiFi DNA Assembly Cloning Kit (New England BioLabs). All plasmids were fully sequenced ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNAs were subcloned downstream of a tetracycline response element (TRE) using the Gibson Assembly Cloning kit (New England Biolabs, # E5510S). Mutations were introduced by in vitro mutagenesis using Q5 Site-directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: ... RNA extraction was done with TriZol from the 12 fractions collected and then samples were cleaned up using the Monarch® RNA Cleanup Kit (50 μg) from New England Biolabs (NEB). Optical densities (OD260 ...
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bioRxiv - Synthetic Biology 2023Quote: ... we constructed the genetic sequence of the major shell protein of 50nmGVs using Q5 DNA polymerase and Gibson assembly kits (New England Biolabs Inc.)65 ...
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bioRxiv - Plant Biology 2023Quote: Constructs of MBP-DspE and MBP-DspEΔβ-barrel were made by the infusion cloning methods using NEBuilder HiFi® DNA assembly kit (New England Biolabs) with the following primer sets:
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bioRxiv - Plant Biology 2023Quote: ... The purified total RNA was used to synthesize cDNA using LunaScript® RT SuperMix Kit (New England Biolabs, Catalog number E3010). The synthesized cDNA was analyzed using qPCR using Luna® Universal qPCR Master Mix (NEB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... or NEBNext Ultra FS DNA library kits (paired-end 150bp reads, average insert size 155 ± 105 bp, New England Biolabs, MA)) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... the BRE-Citrine was replaced with CAGA12-mCherry using the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, E5520S). For ease of readability these reporters are referred to as BRE-YFP and SBE-RFP in the manuscript.
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bioRxiv - Developmental Biology 2023Quote: ... A short version of enhD1 luciferase vector was mutated with use of a gBlock (IDT) and NEBuilder HiFi DNA Assembly kit (NEB, E2621). Luciferase reporter vectors were co-transfected with pNL1.1.PGK[Nluc/PGK] (N1441 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sequencing libraries were generated with a NEBNext Ultra Directional RNA Library Prep kit according to the supplier’s directions (New England Biolabs, Beverly, MA). Sequencing was performed as previously described on a NovaSeq 600 instrument (Illumina ...
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bioRxiv - Genetics 2023Quote: ... strand-specific mRNA libraries were generated using the NEBNext Ultra II Directional RNA library prep Kit for Illumina (New England BioLabs #E7760), and mRNA was isolated using Poly(A ...
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bioRxiv - Genetics 2023Quote: These three parts were assembled together with the pJmp3 backbone using the NEBuilder HiFi DNA Assembly cloning kit (NEB cat # E5520S) and transformed to DH5α competent cells ...
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bioRxiv - Microbiology 2023Quote: All parasite materials were centrifuged at 1,000 g for 2 min and total genomic DNA was isolated from the resulting pellets using the Monarch Genomic DNA Purification kit (New England Biolabs, Australia). For the ovine ...
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bioRxiv - Genetics 2023Quote: ... 100ng of each mRNA sample was used to prepare libraries with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760S) according to the manual ...
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bioRxiv - Genomics 2023Quote: ... with the exception that elution of the Tn5 treated DNA was performed using the Monarch PCR and DNA clean-up kit (NEB T1030S) following a 5:1 ratio of Binding Buffer:Sample ...
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bioRxiv - Genomics 2023Quote: ... the samples were incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was sonicated using Qsonica sonicator (Q8000R) and the sonicated DNA was used to prepare libraries using the NEBNext UltraII DNA library prep kit (NEB #E7645L). Paired end sequencing of the libraries was performed using Illumina NextSeq 550 platform and at least 3 million paired end reads were obtained for each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... truncations were then constructed from this plasmid by site-directed deletion of the appropriate regions of the SFPQ ORF using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The mammalian expression construct encoding GFP-tagged full-length SFPQ was described previously (Huang et al ...
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bioRxiv - Cancer Biology 2023Quote: ... The U6 promoter was deleted and replaced with an XbaI site using NEB Q5 site directed mutagenesis kit (NEB Cat# E0554S) with primers forward ...
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bioRxiv - Genetics 2023Quote: ... and inserted to AatII site on pHACK-GAL4>nlsLexA::GADfl (v1) using the HiFi DNA Assembly Cloning Kit (New England BioLabs, E5520S). The resulting construct carrying both 3xP3-RFP and yellow transgene markers ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the pML104-GAL1 vector has a guide sequence 5’- CTCTTAAATTATAGTTGGTT-3’ introduced by Q5® Site-Directed Mutagenesis Kit (New England Biolabs) (Hu et al. ...
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bioRxiv - Microbiology 2023Quote: ... AOB and AOA amoA genes were created using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, MA) according to the manufacturer’s guideline ...
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bioRxiv - Plant Biology 2023Quote: ... The DNA fragment was introduced into pGreen0179_YFP-(AauI)::35St with EXP7a promoter by using a NEBuilder HiFi DNA Assembly kit (NEB, MA, USA), and the resulting construct was transformed into Arabidopsis Col-0 by floral dip (Clough and Bent ...
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries for genome wide DNA methylation profiles were generated from 200 ng of genomic DNA using NEBNext® Enzymatic Methyl-seq Kit (New England Biolabs). These libraries were sequenced on an Illumina NextSeq 2000 to generate 100 bp paired end reads.
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bioRxiv - Microbiology 2023Quote: All clonings were carried on in a Gibson assembly-like reaction with the NEBuilder® HiFi DNA Assembly kit (NEB, E2621) and into plasmids derived from pMM704 or pMM731 (17) ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA libraries from at least three replicate individuals per sex for each lineage (Table S1) were prepared from gametes using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs). Mature gametophytes release gametes just after the medium renewal ...
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bioRxiv - Molecular Biology 2023Quote: ... A PCR-amplified DNA fragment of pcDNA3-DNMT1 was generated using Q5 Site-Directed Mutagenesis Kit (E0554S, NEB, Ipswich, MA, USA). The primers used in this process are described in Supporting Information ...
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bioRxiv - Microbiology 2022Quote: ... Residues that failed this method were substituted by re-cloning the gene as two overlapping fragments using NEBuilder® HiFi DNA Assembly Cloning Kit (NEB) according to supplier protocol and recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... The APOBEC3A and BSA materials used were purchased from the NEBNext© Enzymatic Methyl-seq Conversion Module kit from New England Biolabs (NEB). The reaction volume was then scaled down to 15.5 uL and modified from the original deamination protocol from the kit ...
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bioRxiv - Biochemistry 2023Quote: ... The mutant and wild-type target RNA were subsequently amplified using either the NEB Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB E3019), Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB M3019 ...
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bioRxiv - Immunology 2023Quote: ... 250 ng of input DNA was prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, #E7645S) and sequencing adaptors were ligated using NEBNext Multiplex Oligos for Illumina (#E7500S ...
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bioRxiv - Bioengineering 2023Quote: ... and a gBlock (purchased from Integrated DNA Technologies [IDT]) containing an anhydrotetracycline (aTc) inducible promoter (pLTetO-1) were inserted via HiFi Assembly (HiFi Assembly Kit (NEB#E5520S)) into a PCR-ed fragment of plasmid pBbA2c-RFP (Addgene #35326 ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng of DNA was used for library preparation according to NEBNext® Ultra II DNA Library Prep Kit (NEB, E7645S). Purity and size distribution of the libraries were estimated using Fragment Analyzer ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng of DNA was used for library preparation according to NEBNext® Ultra II DNA Library Prep Kit (NEB, E7645S). Purity and size distribution of the libraries were estimated using Fragment Analyzer ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng of DNA was used for library preparation according to NEBNext® Ultra II DNA Library Prep Kit (NEB, E7645S). Purity and size distribution of the libraries were estimated using Fragment Analyzer ...
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bioRxiv - Developmental Biology 2022Quote: 50mg of muscle tissue were obtained from a euthanized adult female sugar glider and high-molecular weight DNA was extracted using the Qiagen MagAttract HMW kit (NEB 67563). DNA was quantified using the Qubit DNA HS kit (Thermofisher Q32851 ...
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bioRxiv - Genomics 2022Quote: ... Libraries were prepared by the Cornell TREx facility using NEBNext Ultra II Directional Library Prep Kit (New England Biolabs, Ipswich, MA) with ribosomal RNA depletion ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We prepared genomic DNA libraries for the 16 samples using NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, Inc). We quantified the quality of the genomic DNA library using the Agilent 2100 Bioanalyzer system located at the Genome Analysis Core Laboratories at Georgia Institute of Technology (Agilent Technologies ...
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bioRxiv - Genomics 2023Quote: ... RNA-seq library preparation was performed with the NEBNext Ultra II RNA Library Prep Kit and sequenced with the Illumina platform (NEB E7770S). Ribosomal RNAs (rRNAs ...
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bioRxiv - Cancer Biology 2023Quote: ... and sequencing libraries were prepared from 5 ng of the purified amplicon using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, E7370L) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... MNase activity was stopped and chromatin fragments released into supernatant were purified using the NEB Monarch DNA Cleanup Kit (NEB, T1030) per manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: DNA from in vitro and in vivo experiments was sent to Novogene (Sacramento, CA) and library preparation was performed using the NEBNext® DNA Library Prep Kit (New England Biolabs) following the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2023Quote: ... while Icelandic and eastern Greenland rock ptarmigan DNA was extracted using Monach™ Genomic DNA Purification Kit (New England BioLabs, USA). DNA was checked for concentration and purity using a NanoDrop® 2000 spectrophotometer and Qubit® 3.0 fluorometer Quantitation Kit (Invitrogen™) ...
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bioRxiv - Genomics 2022Quote: ... mScarlet and HPRT (housekeeping gene for normalization) levels were then measured by qPCR using the Luna Universal One-Step RT-qPCR Kit (NEB #E3005) with 100ng total RNA per reaction ...
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bioRxiv - Genomics 2022Quote: The extracted genomic DNA was used for library construction using NEBNext® DNA Library Prep Kit (New England Biolabs, MA, USA), and sequencing (150 bp paired end ...
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bioRxiv - Genetics 2023Quote: ... Libraries for sequencing were prepared with a modified version of the Next Ultra II DNA Library Prep Kit for Illumina (NEB, #E7645S). Sequencing was performed on an Illumina HiSeq 3000 ...
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bioRxiv - Genetics 2023Quote: ... Subtraction of poly-A RNA using NEBNext Ultra II with a Poly-A Selection kit (New England Biolabs, Ipswich, MA, USA) and poly-A RNA sequencing were performed at Admera Health (South Plainfield ...
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bioRxiv - Genetics 2022Quote: ... Libraries were prepared from 500ng of the total-RNA sample using the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB #7760) with Poly-A mRNA magnetic isolation (NEB #E7490 ...