Labshake search
Citations for New England Biolabs :
8501 - 8550 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... RNA integrity was validated by gel electrophoresis and 800 ng were reverse transcribed using the LunaScript® RT SuperMix Kit according to manufacturer’s instructions (E3010, New England Biolabs, Ipswich, MA). Real-time PCR was performed using the Blue ŚGreen qPCR Kit (F416 ...
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bioRxiv - Genetics 2022Quote: ... The tRNAs were re-phosphorylated and converted to small RNA sequencing libraries using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs). Size selection of 140-155 bp PCR amplified fragments (corresponding to 19-35 nt tRNA fragments size range ...
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bioRxiv - Physiology 2022Quote: ... 3-10 ng of immunoprecipitated DNA was used to generate adapter-ligated DNA libraries using the NEBNext® Ultra DNA library kit for Illumina (New England Biolabs, E7370L) and indexed multiplex primers for Illumina sequencing (New England Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: ... 100bp strand-specific whole transcriptome libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, Herfordshire, UK), following rRNA depletion with NEBNext rRNA Depletion Kit or Ribo-Zero Gold ...
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bioRxiv - Cell Biology 2022Quote: ... mutated FN constructs were PCR amplified and ligated into BamH1+Xho1 cut NSPI vector using NEBuilder® HiFi DNA Assembly kit (BioLabs, #E5520).
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bioRxiv - Genomics 2022Quote: ... wild-type and mutant NEAT1 RNA fragments were transcribed in vitro using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, #E2040S) and labelled with Biotin using Biotin RNA Labelling Mix (Roche ...
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bioRxiv - Synthetic Biology 2019Quote: ... The three backbone fragments were combined together with the synthesized CggR cis-regulatory element using the NEB Gibson assembly kit (New England Biolabs, MA, US) according to the manufacturer instructions ...
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bioRxiv - Genomics 2019Quote: ... sequencing libraries were constructed with the NEBNext® Ultra™RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, USA), and Illumina PE (150 bp x 2 ...
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bioRxiv - Developmental Biology 2019Quote: ... pOI191 was derived from this mScarlet clone (pOI186) by mutating a single nucleotide to convert mScarlet to mScarlet-I using the Q5 Site-Directed Mutagenesis kit (NEB Cat#E0554). Primers used for cloning:
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bioRxiv - Plant Biology 2019Quote: ... Illumina paired-end libraries were constructed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA), before being sequenced using Illumina HiSeq 2500 platform (Hokkaido System Co ...
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bioRxiv - Molecular Biology 2020Quote: Thermocycler was used to denature and anneal 5 µl of PCR product as recommended by manufacturer (EnGen™ Mutation Detection Kit, NEB, E3321S). Thermocycler was adjusted for heteroduplex formation as following ...
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bioRxiv - Microbiology 2019Quote: ... each of the amplicons were diluted to 0.5 nM and amplified using barcoding primers (1D PCR Barcoding Amplicon Kit, Oxford Nanopore Technologies, [ONT], UK) and LongAmp Taq 2X Master Mix (New England Biolabs, MA, USA) with the following conditions ...
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bioRxiv - Cancer Biology 2019Quote: ... and then performed library preparation using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... as previously described[7] Phage DNA libraries were prepared using NEBNext Ultra II FS library Prep and Kit for Illumina (New England Biolabs, Ipswich, USA). The sequencing was performed on the Illumina iSeq platform as part of a flowcell (2 x 151 ...
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bioRxiv - Genomics 2019Quote: ... cDNA was generated using random hexamer priming and the Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA). We amplified cDNA using the ZikaAsian V1 ZIKV-specific primer scheme [20] ...
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bioRxiv - Genomics 2019Quote: ... Library preparation for sequencing was performed using a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (E7645, New England Biolabs NEB). Samples were sequenced paired-end ...
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bioRxiv - Microbiology 2019Quote: ... The RNAseq libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, Ipswick MA), following manufacture’s recommendation ...
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bioRxiv - Genetics 2019Quote: ... After preparing libraries from these samples according to the manufacturer’s protocols using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA), WGS was performed using a NextSeq500 (Illumina ...
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bioRxiv - Synthetic Biology 2020Quote: ... were PCR amplified from genomic DNA of yeast strain BY474129 and cloned together with the bidirectional inducible Gal1-10 promoter into the PCR amplified pRSII42B backbone with OZL14 and OZL15 via Gibson assembly30 using NEBuilder Kit (NEB, catalog E2621). MMR-DN mutant genes were subsequently generated via site-directed mutagenesis using either Q5 Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Genomics 2020Quote: ... paired-end libraries with insert size of ~300 bp were also constructed using NEB Next® Ultra DNA Library Prep Kit (NEB, USA), with the standard protocol provided by Illumina (San Diego ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and cDNA libraries were made from each sample using the NEBNext RNA Ultra II Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). Barcoded samples were sequenced using the Illumina NextSeq 500 (Illumina ...
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bioRxiv - Genomics 2019Quote: ... the DNA was then converted into double indexed Illumina libraries using the NEBNext® Ultra™ II DNA Library Prep kit (New England Biolabs® ...
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bioRxiv - Genomics 2020Quote: ... and 10-12 day adult TRAP libraries were prepared with the NEBNext Ultra RNA library Prep Kit for Illumina (NEB, product E7530S). ChIP-seq libraries were prepared using the NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... Library quality was assessed by Bioanalyzer and the concentration was determined using the NEBNextv Library Quant Kit for Illumina® (New England BioLabs, E7630). Libraries were pooled and sequenced on the HiSeq4000 machine (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... plasmid encoding GFP-labeled dominant negative mutant version of human Rab5B isoform was introduced to plasmid #1008 using Q5 Site-Directed Mutagenisis kit (NEB, Cat# E0554S) according to manufacturer’s instructions using forward primer #1554 (AGTGGGAAAGaacAGCCTGGTATTAC ...
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bioRxiv - Genetics 2019Quote: We constructed individually barcoded whole genome shotgun libraries using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) and 6-bp custom indexed primers ...
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bioRxiv - Molecular Biology 2019Quote: ... IVT reactions were performed using the HiScribe T7 High Yield RNA Synthesis Kit using 300 ng of PCR product as template (NEB Cat. #E2040S). After an incubation for 16 hours at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA libraries for Illumina sequencing were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, MA, USA) following the manufacturer’s protocol in 0.5 of the recommended volume (due to low RNA quantity in such samples as shoot apical meristem) ...
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bioRxiv - Developmental Biology 2019Quote: RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Illumina paired-end libraries were constructed with the NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... plus 280 nM for each primer and the standard reagents in the Phusion High-Fidelity PCR Kit (New England BioLabs, Ipswich, MA). Samples were then cycled under the following qPCR conditions ...
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bioRxiv - Microbiology 2020Quote: ... Five hundred nanograms total RNA was then reverse-transcribed in 20 μl reaction volume using the LunaScript RT SuperMix Kit (E3010, New England Biolabs, MA, USA). Quantitative PCR was performed by transferring 2 μl of the RT mix to the qPCR mix prepared with Luna Universal qPCR Master Mix (M3003 ...
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bioRxiv - Microbiology 2021Quote: ... and library construction were done using NEBNext® µLtra™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswitch, MA, USA) following the manufacturer’s recommendation and starting with 3 μg of RNA per sample ...
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bioRxiv - Microbiology 2021Quote: ... HA tag sequences were added to the 3’ end of the hACE2 transgenic cDNA using a Q5® Site-Directed Mutagenesis Kit (NEB#E0554S) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Libraries were made using polyadenylated mRNA isolation and employing NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). Three samples had lower RNA amounts and only a higher efficiency bead capture was employed for those ...
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bioRxiv - Biochemistry 2020Quote: ... Mutations were introduced into the ATP11A ORF using a The Q5® Sit-direct mutagenesis kit (New England Biolabs, Ipswich, MA, USA) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... Up to 10 ng of cDNA was used for the Illumina sequencing library construction using NEBNext® Ultra™ DNA Library Prep Kit (NEB). Paired ends sequencing was performed on NextSeq 500 (Illumina ...
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bioRxiv - Physiology 2019Quote: ... Systematic promotor truncations were generated using the Q5 Site-Directed Mutagenesis Kit according to manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Plasmid constructs were introduced via Neon electroporation (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... The sample preparation was performed according to the protocol “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (NEB #E7760S/L). Briefly ...
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bioRxiv - Genomics 2020Quote: ... A cDNA library with insert sizes of 350-400 bp was prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, MA, USA) and paired-end sequenced on the HiSeq X ten platform (Illumina Inc. ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was synthesized from extracted RNA using the ProtoScript First Strand cDNA Synthesis Kit (#E6300, New England Biolabs Inc, Beverly, MA, USA). CDKN1A expression levels were measured using quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2019Quote: ... Measurement of differential gene expression was conducted using a qTower 2.2 (Analytik Jena, Jena, Germany) with the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswitch, USA). Primer pairs used for the analysis are listed in Table S3 D ...
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bioRxiv - Microbiology 2019Quote: ... truncation mutations in both bacterial and mammalian expression vectors were generated using the NEB Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA). All constructs were sequence-verified using universal primers specific to the construct backbone (GENEWIZ ...
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bioRxiv - Plant Biology 2019Quote: ... The fragmented gDNA was used for the generation of libraries with the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, MA, USA). We used different combinations of primers as barcodes to pool two different libraries into one SMRT cell ...
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bioRxiv - Genomics 2019Quote: ... expressing ATG7 isoform 2 was a gift from Toren Finkel (Addgene plasmid #24921).31 The plasmid pCMV-myc-Atg7(1) expressing ATG7 isoform 1 was derived from pCMV-myc-Atg7(2) using the Q5 site-directed mutagenesis kit (NEB, Ipswich, MA). Subsequently ...
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bioRxiv - Neuroscience 2019Quote: ... and libraries were constructed in technical triplicate using NEBNext Poly(A) Magnetic Isolation Module and NEBNext UltraII RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA), with size selection by AMPure XP beads (Beckman Coulter ...
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bioRxiv - Biochemistry 2019Quote: ... The gene was cloned into the first open reading frame of a pCDF-Duet vector using Gibson Assembly Kit (NEB, Ipswich, MA).[23 ...
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bioRxiv - Genomics 2019Quote: RNA libaries were prepared using both E7530L and E&335L NEBNext® Ultra™ RNA Library Prep Kits (New England Biolabs, USA) to generate fragment sizes of 351-371 bp ...
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bioRxiv - Genomics 2021Quote: ... One microgram of mRNA was used as a template for first-strand cDNA synthesis using NEBNext® Poly(A) kit (NEB #E7490, New England Biolabs, Inc.) and NEBNext® ultra™ II directional RNA library prep kit for Illumina (NEB #E7760 ...
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bioRxiv - Microbiology 2021Quote: ... This PCR product was cloned into pBHM2403digested with NotI and SacII by Gibson assembly using the NEB Hifi DNA Assembly Kit (New England Biolabs, Ipswich, MA). To generate a sgRNA expression cassette ...