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Citations for New England Biolabs :
8451 - 8500 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: B cells were cultured for 48 hours before total RNA was extracted using Quick-RNA™ Microprep Kit (ZymoResearch) and mRNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation (New England BioLabs). Stranded Illumina libraries were prepared with Swift Rapid RNA Library Kit according to the manufacturer’s instructions (Swift Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... The pACYC184::attI1 recipient plasmid was created by assembling the attI1 sequence (from R388) into the pACYC184 plasmid backbone using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, United States). The PCR products required for the assembly were generated using the attI1_fw/attI1_rev and pACYC184_backbone_F/pACYC184_backbone_R primer pairs ...
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bioRxiv - Neuroscience 2022Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... 150 ng of RNA per sample was employed in the generation of libraries using the NEB Next Ultra Pure RNA library kit for Illumina (NEB, cat # E7770S) per manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... and a PCR-free library was obtained with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs). Approximately 200 million of 150 bases pair-end reads were generated using the HiSeq X System (Illumina) ...
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bioRxiv - Genetics 2022Quote: ... the truncated versions of cwr-1 and the histidine mutant plasmids were prepared using the Q5 Site-Directed Mutagenesis Kit (E0552S NEB, Ipswich, MA). Gibson assembly (E2611S NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... The library for RNA-Seq was prepared by using NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs, Ipswich, MA). During the second cDNA synthesis dUTP was incorporated to maintain strand specificity ...
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bioRxiv - Cell Biology 2022Quote: ... The sample preparation was performed according to the protocol “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (NEB #E7760S/L). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA libraries were constructed using the NEBNext® Ultra(tm) II Directional RNA Library Prep Kit (New England Biolabs, Inc., Massachusetts, USA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: One microgram of total RNA from each sample was subjected to cDNA library construction using a NEBNext® Ultra non-directional RNA Library Prep Kit for Illumina® (New England Biolabs). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by column purification and on-column DNase digestion according to the TRIzol two-step protocol from the Monarch total RNA miniprep kit (NEB, cat. T2010S) or PureLink RNA mini kit (Ambion ...
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bioRxiv - Molecular Biology 2020Quote: ... The libraries of input fractions from Figure S8 were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for illumina (NEB).
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bioRxiv - Neuroscience 2020Quote: ... Sequencing libraries were prepared from a starting amount of 100ng total RNA using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina (E7370L, New England Biolabs) using ribosomal depletion as a selection method ...
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bioRxiv - Microbiology 2020Quote: ... 1-3 μg RNA was used to generate sequencing libraries with NEBNext Ultra™ RNA Library Prep Kit for Illumina (#E7530L, NEB, USA) with poly(A ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg total RNA with RIN value above 7 was used for library preparation by Genewiz using NEBNext® UltraTM RNA Library Prep Kit for Illumina® and NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Size selection of Adaptor-ligated DNA was then performed using AxyPrep Mag PCR Clean-up (Axygen) ...
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bioRxiv - Plant Biology 2021Quote: ... Library concentration was further quantified by qPCR using the NEBNext® Library Quant Kit for Illumina® (New England BioLabs®, Inc.). Libraries were sequenced on one lane of the Illumina® HiSeq4000 platform to generate 150-bp paired-end reads.
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bioRxiv - Molecular Biology 2020Quote: ... sgRNA target site mutation in mouse Thap1 cDNA was generated by Q5 Site-Directed Mutagenesis Kit (NEB, see Table S3 for primers). WT or Thap1-/-Brca1Δ11 MEFs (1 × 106 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 3 μL of factor mix (with RNA polymerase, and transcription/translation factors in 10 mM Mg2+) from the PURExpress® Δ Ribosome Kit (New England Biolabs). The reaction buffer was based on Shimizu et al ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries for sequencing were prepared using NEBnext Ultra II directional RNA library prep kit for Illumina (New England Biolabs, Cat. No E7760L). Libraries were sequenced on an Illumina HiSeq 1500 instrument at the Laboratory of Functional Genomic Analysis (LAFUGA ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA libraries were generated using 10ng purified ChIP-DNA and the NEBNext®Ultra IIDNALibrary Prep Kit for Illumina® (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and NEBNext Ultra II FS DNA library kit for Illumina with 384-unique indexes (New England Biolabs, Massachusetts, USA; Cat. No: E6617L). Libraries were sequenced using the HiSeq X10 with 150 bp paired-end chemistry (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... while libraries for Illumina sequencing were prepared by using a NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The synthesized oligo pool of CRISPRa library was PCR amplified with primers (table S5) including the BsmBI recognition sites using Phusion®High-Fidelity PCR Kit (NEB, E0553L). After purification with DNA Clean & Concentrator-25 (Zymo Research Corporation ...
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bioRxiv - Microbiology 2021Quote: ... resulting in ∼1 ml of turbid bacterial culture which was subsequently used as input for plasmid purification by miniprep kit (New England Biolabs, cat#T1010S). The pooled plasmid library DNA for each library was eluted in 35 μl of elution buffer and quantified ...
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bioRxiv - Microbiology 2021Quote: ... and ∼ 90 ng was used for one-step RT-qPCR using Luna® Universal one-step RT-qPCR kit (New England BioLabs Inc.) [10 min ...
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bioRxiv - Immunology 2021Quote: ... The linearized vector and the synthesized fragment in a 1:2 molar ratio were assembled with the NEBuilder HiFi DNA Assembly Kit (NEB, Ipswich, USA) at 50°C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: A total amount of 0.4 ug of RNA was used for cDNA library construction at Novogene using an NEBNext® Ultra 2 RNA Library Prep Kit for Illumina® (cat NEB #E7775 ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNAs were reverse transcribed to cDNA using ProtoScript II reverse transcriptase with oligo dT and random primers (First Strand cDNA Synthesis kit New England Biolabs, Ipswich, MA). RT-PCRs specific for the Cas9 or actin gene of B ...
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bioRxiv - Microbiology 2020Quote: ... and one fragment of about 320 bp of the 3’-terminal region by 3’ RACE were amplified using Phusion High-Fidelity PCR Kit (New England Biolabs, MA, USA) under the following conditions [98°C ...
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bioRxiv - Plant Biology 2021Quote: ... mRNA was enriched with oligo(dT)-beads and cDNA was synthesized with random hexamer primers with the NEB Next Ultra RNA Library Prep Kit (NEB, Ipswich, USA). All libraries were sequenced using the Illumina NovaSeq6000 platform in paired-end mode with a read length of 150 bp.
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bioRxiv - Microbiology 2020Quote: ... The products of this reaction were prepared for Illumina sequencing using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was performed in a two-step manner using a ProtoScript First Strand cDNA synthesis kit (New England Biolabs, Cat#E6300S) and iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting single stranded cDNA was further In-vitro transcribed (IVT) using HiScribe T7 Quick High Yield RNA Synthesis Kit according to the manufacturer’s protocol (New England BioLabs Inc, NEB #E2050). The amplified RNA was than purified using Monarch RNA Cleanup Kit (New England BioLabs Inc. ...
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bioRxiv - Microbiology 2021Quote: ... samples were harvested and RNA was isolated and reverse trancripted in cDNA following protocol manufacturer (LunaScript™RTSuperMix Kit, New England Biolabs Inc). The 20 μL reaction consisted in 4 μL of RT Mix completed with 16 μL of water with 100 ng of RNA ...
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bioRxiv - Microbiology 2021Quote: ... Samples were treated with chloroform and the aqueous phase was used in the Monarch® RNA Cleanup Kit (New England Biolabs Inc).
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bioRxiv - Cell Biology 2020Quote: ... of full-length SEC16A were generated by mutating 10 nucleotides in the silencer-select Sec16A siRNA (5’-CGGAGCUUCUGUUACGAGATT-3’, siRNA id: s19236, Thermo-Fischer Scientific) binding site using Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) following manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... pBGC was constructed by joining the amplified pBGT backbone and the gfp terminator region using the Gibson Assembly Cloning Kit (New England BioLabs, MA, USA). Resulting reaction was transformed by heat shock into NEB 5-alpha Competent E ...
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bioRxiv - Microbiology 2020Quote: ... we prepared a fragmented DNA library using a NEBNext Ultra II FS DNA Library Prep Kit for Illumina according to the manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Sequencing of a paired-end 2 × 150 bp mode on a HiSeq X system (Illumina ...
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bioRxiv - Immunology 2019Quote: Plate based scRNA-seq was performed with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs Inc, E6420L). Cells from donor 364b were snap frozen in 10% DMSO in 90% BSA ...
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bioRxiv - Microbiology 2021Quote: ... were prepared using NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina modules E7810L and E7595L (New England BioLabs) following the manufacturer protocol for use with inputs ≥ 100 ng with modifications to eliminate PCR-amplification steps.
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bioRxiv - Immunology 2021Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Bioengineering 2021Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Microbiology 2021Quote: The DNA library was prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions and sequenced using the Illumina MiSeq platform with a read length of 2 × 150 bp (Illumina ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... <200nt isolates were immediately frozen at -80⍰°C while >200nt isolates were used as template for cDNA synthesis (LunaScript RT Mastermix Kit, New England Biolabs, MA, USA) and subsequent doublesex sexing PCR as previously described (25) ...
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bioRxiv - Microbiology 2022Quote: ... The rRNA-depleted total RNA was subjected to library preparation using NEBNext® Ultra™ II RNA Library Prep Kit (E7700, NEB) and barcoded with NEBNext Multiplex Oligos for Illumina (E7730 ...
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bioRxiv - Microbiology 2022Quote: ... All capped viral RNA was in vitro transcribed from the corresponding linearized plasmid using the HiScribe T7 antireverse cap analog (ARCA) mRNA kit (New England Biolabs, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: ... total RNA libraries were built using the NEBNext Ultra II library prep kit for Illumina (New England BioLabs inc., product code: E7775) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... the plasmid library was linearized by BsmbI digestion and ligated with TmU fragments with Quick ligation™ kit (New England BioLabs, M2200S) for 5 minutes at room temperature ...
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bioRxiv - Genomics 2022Quote: Poly-A libraries (figure 1 and related data) were constructed with the NEBNext Ultra RNA library prep kit for Illumina (New England Biolabs, Ipswich, USA), following the manufacturer’s instructions ...